LOCUS       NZ_CP056262             9294 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Klebsiella aerogenes strain RHBSTW-00898 plasmid pRHBSTW-00898_3,
            complete sequence.
ACCESSION   NZ_CP056262
VERSION     NZ_CP056262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148733
            Assembly: GCF_013727635.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T3-00898
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.0111481093368x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013727635.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 05:03:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,270
            CDSs (total)                      :: 5,148
            Genes (coding)                    :: 5,071
            CDSs (with protein)               :: 5,071
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 36 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 11 of 77
            Pseudo Genes (multiple problems)  :: 9 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056262.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00898"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:548"
                     /plasmid="pRHBSTW-00898_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(8370..9294,1..761)
                     /locus_tag="HV316_RS26240"
                     /old_locus_tag="HV316_26255"
     CDS             join(8370..9294,1..761)
                     /locus_tag="HV316_RS26240"
                     /old_locus_tag="HV316_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            771..1028
                     /locus_tag="HV316_RS26245"
                     /old_locus_tag="HV316_26260"
     CDS             771..1028
                     /locus_tag="HV316_RS26245"
                     /old_locus_tag="HV316_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            1113..1262
                     /locus_tag="HV316_RS26250"
                     /old_locus_tag="HV316_26265"
     CDS             1113..1262
                     /locus_tag="HV316_RS26250"
                     /old_locus_tag="HV316_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2743..4707
                     /locus_tag="HV316_RS26255"
                     /old_locus_tag="HV316_26270"
     CDS             2743..4707
                     /locus_tag="HV316_RS26255"
                     /old_locus_tag="HV316_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            4707..5438
                     /locus_tag="HV316_RS26260"
                     /old_locus_tag="HV316_26275"
     CDS             4707..5438
                     /locus_tag="HV316_RS26260"
                     /old_locus_tag="HV316_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            5445..5975
                     /locus_tag="HV316_RS26265"
                     /old_locus_tag="HV316_26280"
     CDS             5445..5975
                     /locus_tag="HV316_RS26265"
                     /old_locus_tag="HV316_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181482081.1"
                     /translation="MALYMVFIEDAENRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6003..6182)
                     /locus_tag="HV316_RS26270"
                     /old_locus_tag="HV316_26285"
     CDS             complement(6003..6182)
                     /locus_tag="HV316_RS26270"
                     /old_locus_tag="HV316_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6251..6628
                     /locus_tag="HV316_RS26275"
                     /old_locus_tag="HV316_26290"
     CDS             6251..6628
                     /locus_tag="HV316_RS26275"
                     /old_locus_tag="HV316_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(6690..6989)
                     /locus_tag="HV316_RS26280"
                     /old_locus_tag="HV316_26295"
     CDS             complement(6690..6989)
                     /locus_tag="HV316_RS26280"
                     /old_locus_tag="HV316_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6979..7242)
                     /locus_tag="HV316_RS26285"
                     /old_locus_tag="HV316_26300"
     CDS             complement(6979..7242)
                     /locus_tag="HV316_RS26285"
                     /old_locus_tag="HV316_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(7548..7967)
                     /locus_tag="HV316_RS26290"
                     /old_locus_tag="HV316_26305"
     CDS             complement(7548..7967)
                     /locus_tag="HV316_RS26290"
                     /old_locus_tag="HV316_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     BGC             3370..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1366..1944)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4708..5435
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 aggaagaaaa gcgtcgccag caggcatggg acgctgctca cccggaagag gggctgaaaa
       61 gagagtatga taaagcgaaa gctgagctgg atgctgaaga taaaaatatt gcgaccttaa
      121 acagtcgcat tgcatcgaca gagaaggcga tccccggtgc aagggctgct gttcaggaag
      181 ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga ttttgtgact tataacccgc
      241 ctcatgaata tggctccggg tggcaggatc aggttcgcta tcttgataag gatattcaga
      301 atcagaatga gaaattaaaa gctgcccaga catctttaaa cgaaatgaat gaatccttat
      361 ccagggataa ggctgcgctt tccggggcga tggagagccg gaaacaaaag gagaaaaaag
      421 cgaaggatgc agaaaataaa ttaaatgagg aaaagaaaaa acctcgtaag ggaactaaag
      481 attacggcca tgattatttt cctgatccca agactgaaga tattaagggg ttgggagagt
      541 tgaaagaggg taaacctaaa acccctaaac aaggtggtgg tggtaagcgg gcccgatggt
      601 atggtgataa aaagcgtaaa atttatgaat gggattccca gcacggtgag cttgaagggt
      661 accgcgccag tgatggcgaa cacctcgggg cattcgatcc aaaaacgggt aagcaggtta
      721 aagggccgga tccaaaacga aacattaaaa aatatcttta agaggtaaat atggggctta
      781 aattacatat tcattggttt gataagaaaa ccgaagagtt taaaggtggt gaatactcaa
      841 aagacttcgg tgatgatggt tctgtcattg aaagtctggg gatgccttta aaggataata
      901 ttaataatgg ttggtttgat gttgaaaaat catgggtttc gatattacag ccacacttta
      961 aaaatgtaat cgatattagt aaatttgatt actttgtatc ctttgtttac cgggatggta
     1021 actggtaagc aaaagttatc aaggatgatt tgaaatacag acttccggtg ttcagggatg
     1081 aataccgggg tttttaaata cagggaaggt atatgaaaaa ggtaaaggtt gtttttcttt
     1141 ttatattgat tttttcaggc tttcttctgg tcgcatgtca ggcaaactat atccgggatg
     1201 ttcagggtgg aacggtggca ccatcgtcct cctctgaact gacggggatc gcggttcagt
     1261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct tttgccctgt
     1321 aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt ggggaactgc
     1381 ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc
     1441 cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa
     1501 ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gcttttccgg gttggactca
     1561 agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggggttc ttgcttacag
     1621 cccagcttgg agcgaacgac ctacaccgag ccgagatacc agagtgtgag ctatgagaaa
     1681 gcgccacact tcccgcaagg gagaaaggcg gaacaggtat ccgggaaacg gcagggttgg
     1741 aacaggagag cgcaagaggg agcgattcat cggaaacggt gatgatcttt aagtcctgtc
     1801 gggtttcgcc actcctgact gattcatggt tgagccaccg gctcccacag atgcaccgaa
     1861 aaagcgtctg tttatgtgaa ctcagtcagg agggcggagc ctatggaaaa acgccacctg
     1921 cgtggccttg ccgttttgcc tcacatgtta gtcccctgct tattcacgga atctgtgggt
     1981 aactttgcat gtgtccgcag cgcccgccgc agtctcacgg ccggagcgta gcgaccgagt
     2041 gagcgaggag gcgcaatttc cccggcacct acaagacgga caccggacgc gctgatgtgg
     2101 catattgtcc cctgattaag tctttatatt ccctgataat aaattaccag gataggtgaa
     2161 tcatatcggt ttttcgctga acattttacg ttaacctatc tctgttgctt ttttctcctt
     2221 acggagaaat acaggtcatc cccgcgaggg cgtggctgac ctcaggggga tatcccctgc
     2281 ttacgctggc tggacgccac cgacgcacgg tctctccccc tctttctggc tccgtccggc
     2341 tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc tgaccaccca
     2401 caaaaatcca cggtgctgga cgcacctggc gaagcagcaa agctgctcct gattttgcgg
     2461 gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga aaagaccgca
     2521 ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct ccggggctgc
     2581 gccttttccg catggggcgg aaagaggctg gcctccgtgg tgaaagctga acgcaatcac
     2641 cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc
     2701 tgcacatcac gccggcaaac catcgtctga aggagagaga acatgtttaa tacggattcg
     2761 cttgcctgga agtggcacat tccggagctg gcccggcaga tttatccgtg ggtcagctgg
     2821 ctgtaccagg agtattacgg tctggccggg gctttcctag ttatcggcct gtttttcggt
     2881 ctggctgaac tttgggccgg acggatgcgt tttgtggggc tgagtcataa ggagcgacgg
     2941 gagtatgccg atcgctataa gcgcgataat aaacggccag cgccattttc ggtgctggcc
     3001 tcgctggatt cgcccgggcg gatggtcagg gcacgtcgac atgtctggaa ctacgcgtac
     3061 tggctgaccg gcgcggccgg tctggccctt ctgggggggc tggttgtcgc cgtgtttatc
     3121 gagagctggg gagaaaccgt cctgcgtccg gacctctacg caaacgcaga ttattacctg
     3181 ctgcagccgg ctaacctgtt cgggattgcc tggtgcgcgg gcgggctggc tgtcgggatt
     3241 gtgttggcgc ggctgctggc gttcctgctg cttgacggtc attttgcggc tgctgacgag
     3301 gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac aggagagatg
     3361 accgacgtgc gccacctgca tttcggggag ccggtaccgg ttaatgcgct ggccgatttt
     3421 tcctcggagc aggcccggca ggagcaggcg gtgtttctgg gtaaggatga gcagggcaag
     3481 ccggtgctgg ttccgcgtga tacctggcgc aagaccaaca ttcagatcct cggcctgccc
     3541 gggagcggca agagcgtgat ggccaccaac gcgctcattc gctgcgtacg cgatttcggg
     3601 gacgcggtgg tgtacttcga ccccaaaggg gacgcatggg cgccgcacgt cttccgggcg
     3661 cactgtccgg acttcaccct gctggatctg cggccgggga aaccggcgca gctgaatctg
     3721 ttccgcgatc tcgaccagta cgcgctgaaa aacctgctga ttgccgggct taacctgggc
     3781 gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc gaagctgatt
     3841 gcggagcagt ttccgcaggg ggcaaacatt cagcaggtgc tggcggcggc gtacgcgctg
     3901 ccggaggccc tgaaaaagga cgtgaagggg ttgatcacta ggctggaaaa cgtcgctgac
     3961 ctgagcgtcc tgcagacgga cagtggcatc gatgtggcgg ggattatcaa cggcggcggc
     4021 tgcctgtacg ttatcggctc gatggacgac gaggcggtga tccgtgtgca aaaaatgctg
     4081 tttgcccgct gcgcgcaaat catcattgcg cgggacgagt tccgggagtg gccacacgtc
     4141 agcgtgatgc tcgatgaaat taaatatctg ctgtcgaagt atgtactgaa cgcactgggg
     4201 acgctgcgca gccgggactg caatctgttg ctcgcgcacc agtcgctggg cgatttcggg
     4261 cagtgcgggc aggatctgcc cgctgaattt gtgaaaacca cggtactgga taacacgccg
     4321 atccgctggt tttaccgggc ggccagcctg gagtccgcgc agtgggcggc ggggcagacc
     4381 ggggaaatca gggtggacgt ggagcgccgc cgtgccagcc gggaggcggg gaacgtggag
     4441 catatcagcg gggacagctt catccagaag gaggcgcggc cgctgtttga cgtgaacacc
     4501 ctgcaacacc tgccggacgg gttcgcggtc atgaccgggc tgggtgtggc ccggatggcg
     4561 ttcagcagtc cgctgcgggt tgagcgccgg gaaatcccgt tgcagcgttt cccggtgctg
     4621 gcgaaaaccg atcccctggc ggagttccgg cccccggccg acggcggcgg gccgaaagcg
     4681 gatgatgatt ttgcggggat ttactgatgg cgctggagct gtacaacgtc agccacgcga
     4741 aacgacaggc gcggaacgcg gagaaaaccc ggctgaccct gcgctggttg cgggaagagc
     4801 tgtgttccac ggcggagctg gttgcgcggc ggctggggat tgcggcggtg cagccggtct
     4861 accgcttcct cgacagcctg gtggcgaaag ggctgctggt ccggaccaaa tatccggtgg
     4921 acggccgtca ggtcagcgtg tgggggctga cgccgcatgg cgtggcgttc tcgttcgacg
     4981 aggacgaacc gctggcggat gttattccgt ttcagccgtc aagggtgtcg gcagcacatc
     5041 tgccgcaccg gctggccgtg caatccctgc gccttgatat ggaagccaga ggggccaccg
     5101 gctggcgcat tctgcaccgt ctgagtctta aaggcatgaa agtgccggac gccctggcgg
     5161 agctggacgg taaaacggtg gcgtttgagg tggagcgcac cgtcaagagc cgtcgccggt
     5221 atcaggaagt ggtgtccggg tatctgttca accgcaaagc taacggcatc gatgaaatct
     5281 ggtacatctg cccggaccgg gcgacgcagg tcagggtgca gcgggcaatc ctgtcggtgg
     5341 atgaaatcgt gaacccacag accggcgagg cccgcaaaac ggcagagctg gatcgtgaac
     5401 ggttgtttgc gtgctttaaa tttatgacaa cggagtgagg gattatggcg ctgtacatgg
     5461 tatttattga agacgcggag aaccggaacg gcgagcgctg catcatgccg accagcgagc
     5521 atctggaaga tatggtggtg ctcgcctggc agaccggggc gtatgttttc tgttttgtcg
     5581 accgggccca gcatctggcg atgatgaaat ccatcgggat gtatgacgag cggctcaaca
     5641 gcgacgaaaa agccacgtac tttttcactg gtaaaaatcc gcaatgggaa cggaacaaac
     5701 ccacaggaat gaagctgtgt gattacctct atcgcaaggt ggagcggttc gccatcgaga
     5761 acggtgtgca ggtggttgat gccgggaagt gctcaccgga gcagattaac gcgatgattg
     5821 acgtggtgag tgagcggcac ggggaaagcg cggtgaacga gaagctgaaa gcggagctga
     5881 tgcgggaata taacagccgc tattttaacg tgccggtacc tgaactgccg gaggacaaac
     5941 ctgcccgccg gtggtggccg ttcggcaaaa aataaatgcc agcaccggcc atgccggtgc
     6001 tgttattcct ccccgagctt atcccgcatc acctgccaga gtgtttccgc atcctcgtgt
     6061 agccgctcgc acatgtcggc cggggtatcc atatccagct cattcagttt atcgagcaat
     6121 agcagcgact gctccttaat aaatttagcc atattgaggg ctgttttttc ctgttttgtc
     6181 atgattcttt ccttccgggc atggtgtgcg cctgtcaggt atactgtctc aaaaatagcg
     6241 gggtgagaca atggcgtggg tgacggtgag acaggcgacg gaactgacgg gtaaagcccg
     6301 aagttccctg tatcgggata tggcgaaagg ccgcgtatca taccgtacgg aagctgatgg
     6361 cggccgggtg gtggatacca gcgagcttat ccgggtgtac ggtgagttaa gacaggatga
     6421 gactcacgag cgggacgagt tgagactgtc gggtgagact gaaaaaatgg cctccgacgc
     6481 gttgatcgcg gagataaaag cactcaggga ggaggtggcc gggctacggc aggaaatgca
     6541 gtccatgaga ctgctggaac acaaaccgga gacagcagag accccggccg gaacaccgcg
     6601 ccggtggtgg ccgtggggaa aacgctgacg cgctgtgctt gtccaaccgg cgacggccac
     6661 gcaatgaatt gcgtccccgt caccggttat tagagaacaa tatggggatt ctggcgctgg
     6721 ccgaccacgg ccagaacccg gacttttctg gtctggtcat caacatcata gaggacatgc
     6781 tgtcccatca gactgatccg gcgaacgtcc tcgccatagc gtgagtccac ttcatagagt
     6841 cgcgggaagt cggccagttt gtccgccgca gcgagaaccc ggtcggtaac gttctgcgca
     6901 tcccacaggg atgaattttc cgcgatatag tcaaacagcg tcgttacatc ctcgatggct
     6961 ccctgcttcc attccacgtc atacggcatc gcgcagttcc tgataacggc catgcaggcg
     7021 ctggcggagt tgttccgtgg tcatacccgg acgcgtgtca gcacgggcgg cggtcacttt
     7081 ggcgcgaacc tgatcttcat actctgcctg ggccagttct tcgttagcca gtcgctgcaa
     7141 cagcgcagct ttgtccgggc caccttccgg cagtatgcgg ggcaggcttt cagccagttc
     7201 ccgaagaaag ttggcgtttt cgtagtgtgt agtgttgctc attgcggagc ctcccgggtt
     7261 aatgcggcaa tatgaaggaa gtttagcagg gggcggtctg aaagaatatg gtcgtgtcag
     7321 tcaggctgat gaagcagaaa gtcggtaaaa aagggcttac ggcatccatt ttacgtcaaa
     7381 acatatgcca tgcagttgcg tgcataggca tgcattagga taaaatttat cgggcgcgtt
     7441 tccggcagtt ttccgggggg gttgttgcct gttttatccc gtagccgccg gaaacgccct
     7501 gagcccgtct gagcggtgcg cgtaatgacg cgttatggta aatccactta ctgtctgcct
     7561 tcatagccat cgagataaac agctgaaatg gtgtcagcca gtagccttga ttttttcggt
     7621 gtgaggtctt tgctgcggta atcggtaccc gcaaattgtg ttgtgccttg gagtgagttt
     7681 aaaaattcct gactgtgaaa ctgctgaatg cgttgctgca catcactccg tgattttccg
     7741 gtcgctttat cctttgcgcc catatcccag aactgccgga aaaaaggcac catcattcgt
     7801 gttgtgtcag ctttctgggt ggaaaaattg ggatcaaaac cgtcatctga agcatatacg
     7861 taatgccggg tatgctgttc cacgctgccc tttggagcac aggcagtgag agttgctgtc
     7921 gctgttatca tggcgataag acacagtttg ctgccggaac gtctcatgag cgtctcctta
     7981 cctttaattc ttttttctca ttgagttata tatccgcagg ctatcttatc atcagtttct
     8041 gaccgtgttc agtgaccgaa caacccgaac cagacccccc gtttcttttc ctgtcccgga
     8101 gggggctttc tggtatctca tataatgatt tcagtaaaac atactgaggc cggagaacgg
     8161 cgccgtttgt gctggttttt gttgcatctg gaggggattg cgcatggtgt gagtggctgt
     8221 ttttttgatc attttcacaa aaacgcaaat ttacagactt gacatgaaaa acaaggcagc
     8281 gtaattttta tactgtatat aaaaccagtg gttatatgta cagtatttta ttttagttta
     8341 ttgttttaaa agtcaaagag gaaaagtata tgagtggcgg agacggacga ggtccgggta
     8401 attcaggtct gggacacaat ggtggtcagg ccagtgggaa tgtgaacggt acatcaggta
     8461 aaggtggccc ttcatcaggt ggtggtacgg atccaaacag cgggccgggc tggggtacga
     8521 cgcatacacc taacggggat attcataact ataacccggg ggagtttggt aacggcggga
     8581 gtaaacccgg tggtaatggc ggtaacagcg gcaatcatag cgggagctct ggtggcggac
     8641 agtcttcggc caccgcgatg gccttcggtc tgcctgcctt ggccactccg ggggctgaag
     8701 gactggcttt atccgtttcc ggcgatgcgt tgtcggccgc tgttgctgat gtgctggctg
     8761 ccctgaaagg tccgtttaag tttggtctgt gggggattgc gatttacgga gtgctgcctt
     8821 ctgagatagc aaaagatgat ccgaaaatga tgtcaaaaat cgtgacgtca ttgccggcag
     8881 acacggtaac ggagacgccg gtcagctccc tgccgctgga ccaggcgacg gtcagcgtca
     8941 ctaaacgtgt ggcggatatt gtgaaggacg agcggcagca tattgcggtt gtcaccggcc
     9001 ggccaatgag tgttcctgtg gtggatgcga aaccgacaaa acgtccgggg gtattcagtg
     9061 tgtcgattcc gggtctcccg tctctgcagg tgagcgtacc taaaggtgtt ccggccgcga
     9121 aagccccgcc aaaaggcatt attgctgaaa aaggtgattc acgtccggct ggttttacgg
     9181 ccggtggtaa ctcccgtgaa gccgttattc gtttcccgaa agagagcgga cagaaaccgg
     9241 tctatgtgtc ggtgacggat gttcttactc cggcacaggt aaaacagcgt ctgg
//
