LOCUS       NZ_CP056255             1934 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Citrobacter sp. RHBSTW-00903 plasmid pRHBSTW-00903_5, complete
            sequence.
ACCESSION   NZ_CP056255
VERSION     NZ_CP056255.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148737
            Assembly: GCF_013727235.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00903
  ORGANISM  Citrobacter sp. RHBSTW-00903
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T3-00903
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 71.0847053236501x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013727235.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:55:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,583
            CDSs (total)                      :: 5,467
            Genes (coding)                    :: 5,308
            CDSs (with protein)               :: 5,308
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 45 of 159
            Pseudo Genes (incomplete)         :: 120 of 159
            Pseudo Genes (internal stop)      :: 30 of 159
            Pseudo Genes (multiple problems)  :: 31 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056255.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Citrobacter sp. RHBSTW-00903"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00903"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742670"
                     /plasmid="pRHBSTW-00903_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(1339..1934,1..121))
                     /locus_tag="HV320_RS27740"
                     /old_locus_tag="HV320_27745"
     CDS             complement(join(1339..1934,1..121))
                     /locus_tag="HV320_RS27740"
                     /old_locus_tag="HV320_27745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_064761962.1"
                     /translation="MWQARFYQSLPRIVADYPDARWMFLTLTVRNCAIGELGETLNRMN
                     MAFQRLKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHDRWVE
                     LWRECLRVNYDPNVDVRAVKPRKPKDGESLASATAELVRGAVAETLKYSTKPADMVADP
                     EWFLELTKQTHKRRFVATGGALKDILKLDQETDADMVIGDDIPEGDDDGSRIAFEWKTE
                     SKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            complement(378..680)
                     /locus_tag="HV320_RS27745"
                     /old_locus_tag="HV320_27750"
     CDS             complement(378..680)
                     /locus_tag="HV320_RS27745"
                     /old_locus_tag="HV320_27750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     Typing          1422..1613
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.601"
                     /coverage="100.0"
                     /evalue="1.39e-59"
ORIGIN
        1 gtgtctcacc caattccccg atcgcgcagt ttcgcactgt gagggtcagg aacatccagc
       61 gtgcatctgg ataatccgca acaatacgcg gcagggactg ataaaaacgg gcttgccaca
      121 tcagggatcg cctccactgg cagacaggac agtgccggac acggcagaaa tgcgcctcac
      181 gtagcctaag gcgagtttct ccagtttctt tgagggtaga ccaaccaaac ctcagtaaac
      241 cgccacagga agccatacgc gccccgtagc gctcatattc ggctgcgaga aggtaaatcc
      301 cacctacgtc gtcagaaacg cttttgtgag cgtcccaggg cgcgtctgag ggggagtaat
      361 ccgtgaggtt ctggagttta gggttttgca tgcacaccgt cccatgctgg gcgatggctc
      421 atttgacgtg ttatcattgc cgtatcacct gttggttgtc gcttcgaatc gaatttctgc
      481 aggtggtcac acccccgaac gatggccgtc gttgcggggg tcgcttttaa ggcgaccact
      541 cccaacagaa tacttccaaa accttctaca acgcaaataa gccccgaagc cttggtacgc
      601 aaggcttgat gccatttttt atcgccagag ttatccctta agtataaata caaggatcgc
      661 gcgcggatcg aaccagccat tcaacgccca cccgccacca aaaactgcca caacgccagg
      721 agcgaaacag caacgctcaa cgcactatca acatgattaa gaacctcaaa aaacataaca
      781 acctcactcg aatggaattg ggtaaatcta tttagtctgt gatttgcgaa cccatacttt
      841 ttcgcatcac gacagaacca actccattcg ttctggttgt atccggtacc agacaattaa
      901 aaaccgtctt ccaggtggtt ggcctcactt cgtgaatcca accacctcag ccaatttttc
      961 aatcagcaat gtcgtcaatc actaaatcgg aacccactgg caagccagcg ggaccccacg
     1021 atttagagtt tgacgatttc ggaaacggcg agcgctggca gattttaatc tgactgcgtg
     1081 aggtgttggc agggtgtcgg ggcgcagccc tgacgaatcg tttttggcac cgacatcgtg
     1141 atggcggtgt accaaaaaca catcttgtcc caactgagtt cggaaaccga actcagtaat
     1201 tgagtgacgt cactttgcgg cgttagccct atcctgcatc ggagtagaac caataggaca
     1261 agatgtgttt ttggagtacc gccgacaagc ggcgtcctct caaaaacgtc gtggtcaggg
     1321 ctgcgccccg acaccctgtc agtctgattc agctttatct tttgacggtg agcgacggta
     1381 ctttttggat tcggttttcc actcaaaagc aatgcgtgaa ccatcatcat cgccttcggg
     1441 aatatcatcc ccgattacca tatcggcatc agtttcctga tcgagtttca gtatgtcttt
     1501 gagtgcgcca ccggtagcca caaatcggcg cttatgtgtc tgcttagtca attcgaggaa
     1561 ccattcagga tcagcaacca tgtcagccgg cttcgtggaa tatttcaggg tttcagccac
     1621 tgcgccccgt acaagttccg cagttgcaga agccaatgat tctccgtcct taggcttgcg
     1681 gggttttact gctcgaacat caacattcgg atcgtaattc acacgcaaac actctcgcca
     1741 aagctctacc cagcggtcat gtttaacgta atctcgcgtg aacattgagg gaggaaccat
     1801 catcagagtg tgaaagtgcg ggtgcgcact accgtcactt ccacgtgtaa cttccgtggt
     1861 gcggatccag ccctgaacag gtcgaaactc tttgcgatcc ttaagtcgct ggaaggccat
     1921 gttcattcgg ttaa
//
