LOCUS       NZ_CP056227             3223 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00973 plasmid pRHBSTW-00973_6,
            complete sequence.
ACCESSION   NZ_CP056227
VERSION     NZ_CP056227.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148755
            Assembly: GCF_013724605.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00973
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 44.9275864910522x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013724605.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 03:04:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,752
            CDSs (total)                      :: 5,628
            Genes (coding)                    :: 5,483
            CDSs (with protein)               :: 5,483
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 68 of 145
            Pseudo Genes (incomplete)         :: 81 of 145
            Pseudo Genes (internal stop)      :: 33 of 145
            Pseudo Genes (multiple problems)  :: 31 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00973"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00973_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            210..695
                     /locus_tag="HV338_RS28605"
                     /old_locus_tag="HV338_28610"
     CDS             210..695
                     /locus_tag="HV338_RS28605"
                     /old_locus_tag="HV338_28610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            699..914
                     /locus_tag="HV338_RS28610"
                     /old_locus_tag="HV338_28615"
     CDS             699..914
                     /locus_tag="HV338_RS28610"
                     /old_locus_tag="HV338_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1082..1414
                     /locus_tag="HV338_RS28615"
                     /old_locus_tag="HV338_28620"
     CDS             1082..1414
                     /locus_tag="HV338_RS28615"
                     /old_locus_tag="HV338_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            2432..2755
                     /locus_tag="HV338_RS28620"
                     /old_locus_tag="HV338_28625"
     CDS             2432..2755
                     /locus_tag="HV338_RS28620"
                     /old_locus_tag="HV338_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2145..2336
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          complement(1682..2162)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          3..1012
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.265"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2746..3221
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.226"
                     /coverage="99.0"
                     /evalue="5.06e-98"
ORIGIN
        1 ttccgcgctg ccggaagcga agcaggaagc cgcccaggcg attacgagcg gcctgcttgc
       61 ccttgcctca tccggggagc tgaaaacgcg tcaggacgtc actgaggcgc tggaaagcgc
      121 aggttttgag gtggtgcgca ccacaaaaag cagcatcagc attgccgacc cggacggggg
      181 gcgaaacatc cgactgaagg gagccatcta tgaacagtct tttaacgctg gcgaaggact
      241 tagagcagaa atcgaaagcg cagcagcaga ctaccggcga gatgctgaaa gccgcattca
      301 gcgagcacga gaagtctgtc agagcggaac tgagcgaaag cgagaagaga atcagcgccg
      361 ccatcctcga ccacgaccgg aagctgtcct cagccatgag ccagcgcacg aaagggatgc
      421 tgcgcatggt cagccagacg tggctgacca tcgttctggt ctccgcgctg ctgatagcgt
      481 cgagcgcggg cattctgtgg tggcaggggc agcagatact cgagaactat acgaccatcc
      541 gggagcagaa gagcacgcag gccatgctgt cagagaggaa cagcggcgta cagctctcga
      601 cctgcggcga gcagagacgc cgctgcgtga gggtgaaccc ggaagcggga cagttcggag
      661 aggactcgag ctggatgata ctggcgggga aatagcacat gacggagctg gaaaaacagt
      721 tgctgagcgc attagagcag ctacagcagg actactcgaa aaggctggac gagtgggaga
      781 acgccttcgc ggaatggcgg acgatgtctg gtcttattca acgggagaac gcggcgctga
      841 acgagcgcgt cacggtcttg agcaggcagg tgcagagttt gagcgagcag ctgcgccggt
      901 tgtcgaaagg ctgaacgcga tagaaactca ccggcagcag gagcgggcgg tgcagcacca
      961 gaaagcgctg gagctggaac gatcgcagtg gcagcatcat ggtccttcgc tatgataagc
     1021 cgggttctat taattagttg ttatgtaaag atatttaaat ttttcaacat gaggtatttt
     1081 tatgaataca tatattttcc tgggaggggc tattgtggct gaagtcatag gcaccacctt
     1141 tatgaaatat tcagaaggat ttacacgcct gtggccgtct gtaggaactg caataagtta
     1201 ttgcgctgcc ttttatctgc tttcccaaac attggaaaat atacctaccg gtattgctta
     1261 cgcaatttgg tctggtgcgg gaattgtact aataagccta gctggttggt tggttagcgg
     1321 tcaaaaactt gattggcccg caattgcagg aatgttcctt atttgcgttg gagttttggt
     1381 tattaatatt ttttcaaaat cttctgcgca ttaacttttt accgaagcat attggccaaa
     1441 gcaaaaggat ttccttgaga tcctttcttt ctgcgcgtaa tctttttccc tgtaaacaaa
     1501 aaaaccaccg ctaccaacgg tggttcgttt gccggaattc agagctacca actctttgtt
     1561 ccgatggtaa ctggctttca cggagcgcag atacctaaac tgtcctttca gtgtagccgc
     1621 actcaggctt tcacttcaag aactctgtag gcattctcta caatctctgc tccgtgaacc
     1681 cagttaccaa tggctaccgc cactggcgat ttgtcgtgtc tttccgggtt ggactcaagt
     1741 tgatagttac cggaacgggc gtggtagtcg gactgaacgg ggggttcgtg catacagtcc
     1801 atcctggagc gaactgccta cccggaaccg agtttcaggc gtggaatgag aaaccgcggc
     1861 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag
     1921 ccaccagggg gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cgctgatttg
     1981 agcgtcattt tcgtgatact cgtcaggggg gcggagccta tggaaaaacg gcaactccgc
     2041 atccttttct tggcctttcc tgcttcgagc ggcatttgct tatcccgtaa tctgtgagct
     2101 ggtaacgccc gccgcagtct cacggcagga gcgtagcgac cgagtgagcg aggaggcgtc
     2161 atttcatcac cacctgcatt ttgcacttac gcccggcagc cgcatttttc ccctgtcaca
     2221 tgaagcactt tcataaatta ccactccgtc ataacatgtt tagccagtat acactccgct
     2281 agcgctacgt gactggttca gggctgcgcc ccgaaacccg ctaaaaccac tgccgcgtct
     2341 gcggcttgtc caacaccgtg ccgaccggga aaagatttcc cgtccgtccc ggcgttatca
     2401 ggaggccgga ttggcagaca aacgcagcaa aatgctcacc atgtgggtga ctgaggacga
     2461 gcaccggcgt ctgctggagc gctgcgaggg taagcaactc gcggcctgga tgcggcagac
     2521 gtgcctggac gagaagcccg cacgcgccgg caaacttccg tcgatctcgc cggcgctgct
     2581 tcgtcagctt gccggcatgg ggaacaacct taaccagatt gcccgccagg ttaacgccgg
     2641 tggtggaagc ggacacgacc gcgtgcagat tgtcgccgcg ctgatggcca tcgatgccgg
     2701 actcgagcgg ttgcggcatg ccgtactgga aaagggtgct gatgatgatc gttaagtttc
     2761 atcctcgggg gcgcggcggc ggcggcgggc cggtcgatta tctgctgggt aaagatcgcc
     2821 agcgcgacgg cgccagcgtt ctgcagggga agccggatga agtccgggag cttatcgacg
     2881 cctcgcccta cgccaaaaag tacacttccg gcgttctgtc ttttgccgaa caggatttac
     2941 cgcccggcca gcgcgaaaag ctgatggcga gtttcgagcg ggttctgatg cccggactcg
     3001 ataaagacca gtacagcgtg ctgtgggttg agcaccggga caaggggcgg ctggagctga
     3061 acttcctgat cccgaacacg gagcttctga ccggcaagcg gctacagccg tattacgacc
     3121 gcgccgaccg tccgcgcatc gatgcctggc agaccatagt gaacggcagg ctggggctgc
     3181 atgacccgaa cgcgccggag aaccggcggg tgctggtctc gcc
//
