LOCUS       NZ_CP056218             4805 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00982 plasmid pRHBSTW-00982_3,
            complete sequence.
ACCESSION   NZ_CP056218
VERSION     NZ_CP056218.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148760
            Assembly: GCF_013783245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00982
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57.5439619664551x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013783245.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:07:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,275
            CDSs (total)                      :: 5,152
            Genes (coding)                    :: 5,070
            CDSs (with protein)               :: 5,070
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 40 of 82
            Pseudo Genes (incomplete)         :: 41 of 82
            Pseudo Genes (internal stop)      :: 16 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056218.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00982"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00982_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4631..4805,1..287))
                     /locus_tag="HV343_RS26200"
                     /old_locus_tag="HV343_26200"
     CDS             complement(join(4631..4805,1..287))
                     /locus_tag="HV343_RS26200"
                     /old_locus_tag="HV343_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181586210.1"
                     /translation="MSKQNYIQLRSLSDQFYADNKGLQEALDGSNDGKVRGYGIVVIDL
                     NGLMFGIPLRSNLNHKFGFISERSEGNKKGLDYTKALLIQKEEYVSRAYKIPAPEFTHL
                     NDNKEKIQKEFKKFIAKYIDAHNKNDTNILRNYRYSTLKNYHKELGIED"
                     /name="hypothetical protein"
     gene            complement(676..837)
                     /locus_tag="HV343_RS26575"
     CDS             complement(676..837)
                     /locus_tag="HV343_RS26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_346011481.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHAKAPLAAATGKQDVREMYL
                     EFLKWWPN"
                     /name="replication initiation protein"
     gene            1252..1443
                     /locus_tag="HV343_RS26205"
                     /old_locus_tag="HV343_26205"
     CDS             1252..1443
                     /locus_tag="HV343_RS26205"
                     /old_locus_tag="HV343_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042948710.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLTSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1443..1844
                     /locus_tag="HV343_RS26210"
                     /old_locus_tag="HV343_26210"
     CDS             1443..1844
                     /locus_tag="HV343_RS26210"
                     /old_locus_tag="HV343_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_181586211.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLRGEHHSGQVSEQRDTASADQSEQVIRYLKQEIEHLNTLLLSKDSHIDS
                     LKHAMLLLENHQEKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2687..2896)
                     /locus_tag="HV343_RS26215"
                     /old_locus_tag="HV343_26215"
     CDS             complement(2687..2896)
                     /locus_tag="HV343_RS26215"
                     /old_locus_tag="HV343_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_042948706.1"
                     /translation="MKSIKTFVVVTALSMMSFGAFAQSISATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            3372..3782
                     /locus_tag="HV343_RS26220"
                     /old_locus_tag="HV343_26220"
     CDS             3372..3782
                     /locus_tag="HV343_RS26220"
                     /old_locus_tag="HV343_26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042948707.1"
                     /translation="MAFISLSNLAAKTGRIIQSQHVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEDENKKLLSDDSFTQLSEGVIIFKLDDE"
                     /name="hypothetical protein"
     gene            4039..4602
                     /locus_tag="HV343_RS26225"
                     /old_locus_tag="HV343_26225"
     CDS             4039..4602
                     /locus_tag="HV343_RS26225"
                     /old_locus_tag="HV343_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181586209.1"
                     /translation="MRALSVPPSVARANLASKFSSHLKAIALFNTMQDSQVVILSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTVQTVYFIE
                     PEHIEQLYADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIDERFTHMLDELNAA"
                     /name="hypothetical protein"
     Typing          complement(729..1131)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="99.0"
                     /evalue="1.66e-166"
ORIGIN
        1 atcttatacg cccttgatac atactcttct ttctgaatta acagcgcctt agtgtaatcc
       61 aaacctttct tattcccctc tgaacgttca gaaatgaacc caaatttatg attcaaattg
      121 cttcgcaagg gaattccgaa catcaacccg ttgagatcaa taactacgat cccgtaacct
      181 cttacttttc catcattact gccgtcgaga gcctcttgta ggcccttgtt gtcagcgtag
      241 aactggtcgg aaagagacct taactgtatg taattttgtt tgctcatctt taatatccat
      301 tggcattttc tttttaccaa tgtacaaaaa aagcaaagcc ctctgtaaga gagggctttg
      361 cttttctttc gttgagtctt ggatttttta cgtgtggcga actctaccgc acatctttct
      421 ttggaagctt gtttttttta cgtggggctc attcctaccg ccacttcttg agttctttat
      481 atcaaaattt ataggggaag tcaatttagt ggctgttctt gactggactt acttcaccag
      541 cgtaatcttc tcctgaaacg aaaaaaccac cctggcaggt ggtttttcga aggttattta
      601 atcctggcag attatttaac cgtggtaaca gtcttgtgcg agacgtgtca ccaaatccgt
      661 ccttccagtg tagcctcagt taggccacca cttcaagaac tctaaataca tctctcgcac
      721 atcctgttta ccagtggccg ctgccaatgg cgcttttgca tgtcttaccg ggttggactc
      781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcataca
      841 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac
      901 gcggccataa cagcggaatg acaccggtaa accgaatggc aggaacagga gagcgcacga
      961 gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccgccact
     1021 gatttgagcg tcaaattctg tgatgcttgt caggggggcg gagcctatgg aaaaacggcc
     1081 gcgtggcggc ctcctctttt cccctacctt gatcgctcgg tcttctcgag cttttatatg
     1141 catgctcctg ccgctcgccg cagccgaacg accggagcgt agcgactgag tgagcgagga
     1201 agcggaaaag agactggttt tacattgagc actgacgctc tgaggcttct tatgacgaaa
     1261 caacagaaaa cagcgctgaa catggctaaa ttcatccagc atcagtctct gctcctgctg
     1321 gagaagctta acgagcttga tcttgatgct gaggctgacc tttgtgagaa actccacgat
     1381 gatgcggagc atcttttccg tacactgacc tcgagactgg atgcattaca ggatggtaac
     1441 tgatggccag agtgtctatt agtgaggctg cccgactgac gggtaagagt agaacaacac
     1501 tgcacaggct aattaaagca ggtgacctgt ccacctgtag cggtgaacgt aatgcgaaaa
     1561 tggtagatac ttcagagctt ttacgtgtct ttggtccctt tgaacaactg aggggtgaac
     1621 accattctgg acaggtctct gaacagcgtg atacagcctc tgctgaccag agtgaacagg
     1681 tgattcgtta tctgaaacag gagattgaac acctgaacac cttacttttg tccaaggatt
     1741 cgcacattga cagcctgaaa cacgccatgc tccttcttga aaatcaccag gaaaaattaa
     1801 ctgaatcccc ggcgccatgg tggaaattct ggaaaaaatc ctgaccccgt catgcttcag
     1861 ttttcctctc cggccatctc ggggagttcc tgaaccttcc caaaaatcgt cagatctctg
     1921 aacgctcctc accactaaaa atacccacac cccacgcaaa attaaaattt tgaagataac
     1981 tcattaatat cagttagttg tgggttatta aattacgcag tattactttc tgtaaggtga
     2041 atccaagtcg tcggcgattc gtgtacgact tgccgacgac tcgtcataga gtcatcgacg
     2101 actcacttaa gactatcagt aacttatgac gacttgactc tagagtcgaa tcgtcatttt
     2161 ggtgtttttt tgaccttgac gactctagag tcaagtcacg agtcgaatcg ccggtgaatc
     2221 gtgtggtgag tcgtccggcg acttgccata acgtgattgc cggccgtcga cgaacccgat
     2281 tcctgatcgt gctgaaaacc ggttttccgg ctgcgccggc aacgcttcac ggccacgcaa
     2341 taaattgcgt ccccgttcac cgttttcgga taggaagaac gccggaaaca ggacttaccc
     2401 tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg catactcatg catgccgtaa
     2461 aaacagagcc tgcgcgtttc tggcggattt tcgggtggtt tgttgcctgt tttaccggtt
     2521 tcccgtcaga aacgccctga ggccgtttta gcggtgcgta caatcaaggc tttatgttaa
     2581 atgacgaacg caaaaaagcc cccgaagggg ccagagggtt ttcctgttaa ggggcgaaac
     2641 gggccgcctt ccggcgacct gttcggcagc tccctctgcg accatcttat ttggtcagtt
     2701 cagccgtcat gtggacacgg ttattgaact gcgcgctggt gattttgtat gacgcaccct
     2761 gttcagcggc ctgtgcggca atttttgctt cagcacgatc caaggtggag gcggtagcgc
     2821 tgatgctctg agcaaaggca ccgaaagaca tcatggagag agcagtaact acaacaaaag
     2881 ttttgatgga tttcatggtc atattcctta agtcattttg ttctgtatgt ggctttgttg
     2941 ccctgatgtg ataaatgata ggacgaccat gagctgatta aaatcgaaac aatttgcaga
     3001 tgtcattcaa aaaaaaagaa agaaagatag aacgtctccg atctctggtt cttatctacc
     3061 ggccatcgac tagatgccta cgactgaggc ccgatatgaa ggatgtttca tggctattta
     3121 gtggtttttc tcgatgttta cgcattgagt gtttttaaat ctaaaagtga aaatagtctt
     3181 tactaacata aagatcgatc tcgaatcgta ccttgacgag tgttgatacg agcataaatt
     3241 ctgttttttc tgtttttgat gtttttttgt tggatctcga ttgggagaaa atacgaaatt
     3301 tcttgaagat cgagtgggtc cctgggtaca ctatcgatta agaactgatc gacgtctaaa
     3361 cggattgcat tatggctttt atatctctat cgaatttggc agcgaagact ggaaggatca
     3421 ttcagtcgca gcatgttacg gccacgcaaa cgaaaaaaag cactggcgcc gtagagttag
     3481 gcttccgctt aagcaaaagc gctatggacg agaccggaat caccattggg gatcgagtcg
     3541 atattcttta cgacaaagaa gaaaatcagt ggatgattaa gaaaattagt gaaggtggct
     3601 ttggtgtgtc tgggcaaaaa aacaagcacg gtggttactc atcatgtgct gttaggttga
     3661 ccttacgccc tggatttcct cgcctttcgg aggatgagga tgagaacaaa aagttacttt
     3721 ctgatgattc attcactcag ctaagtgaag gtgtaatcat tttcaagctg gatgatgaat
     3781 aggaaagacc aaaaggaaag gcagagttag tgagcgtcgg caaacaatca actaactccg
     3841 ccttctggtc aacacatcac tacctttgga agggtggcgt gttatgggcg gcattatgtc
     3901 cataaacttg cgttctaata aagaagaaat cgtttctttt gaggggggca aacacgacat
     3961 tttctattct gttaggtgcg gtgctattgg ctttggtctc ttaacgttat gtttttaaac
     4021 acatatgaga ctatagatat gagagcatta tcagtaccac cttcagttgc tagggctaac
     4081 ctggcaagta aattctcatc tcatttgaaa gctattgcgc ttttcaacac gatgcaggac
     4141 tcccaagttg tgatcttgtc atcgctgatc gagggacatc acctcaccag ttctggtaac
     4201 agtgtcaaag ctgactttga agtcactcgc ttaccagcca tcattgagat gttagagaaa
     4261 aaatattttt tccctattcg tcatctaaat gtctctgtta aatctgtaac aacaggaaga
     4321 atgactgtac aaactgtcta tttcatagag cctgaacata tcgagcaact ttacgctgat
     4381 cctgagcttg tctttgctaa tcaagaaagg agccttttct ttcgttctct cgaaaaggaa
     4441 ggaaagaatc ttggtaagct tatcgagaag aaaggcagtg tttctcaggc agttctaagt
     4501 cttctacatc atgcttacaa agacaaacct ctttctgacg agatgtggaa agagatagat
     4561 gagaggttca ctcatatgtt ggatgagttg aatgcagcgt gaaggtaagg gcggtctttc
     4621 gaccgccttt ttagtcctct atccctaatt ctttgtgata gtttttcagc gtagagtaac
     4681 gatagtttcg caggatgttt gtatcgtttt tgttgtgagc gtctatgtat ttagctatga
     4741 attttttgaa ttctttttgt attttttctt tgttatcatt gagatgagtg aactctggtg
     4801 ctggt
//
