LOCUS       NZ_CP056207             2520 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Citrobacter sp. RHBSTW-00986 plasmid pRHBSTW-00986_8, complete
            sequence.
ACCESSION   NZ_CP056207
VERSION     NZ_CP056207.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148762
            Assembly: GCF_013783065.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00986
  ORGANISM  Citrobacter sp. RHBSTW-00986
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Shaw,L.P.
  TITLE     REHAB project genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00986
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.3024685258756x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/05/2023 04:44:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,156
            Genes (coding)                    :: 5,003
            CDSs (with protein)               :: 5,003
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 55 of 153
            Pseudo Genes (incomplete)         :: 101 of 153
            Pseudo Genes (internal stop)      :: 29 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            Pseudo Genes (short protein)      :: 1 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056207.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Citrobacter sp. RHBSTW-00986"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00986"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742675"
                     /plasmid="pRHBSTW-00986_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            569..1174
                     /locus_tag="HV345_RS26185"
                     /old_locus_tag="HV345_26205"
     CDS             569..1174
                     /locus_tag="HV345_RS26185"
                     /old_locus_tag="HV345_26205"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012564911.1"
                     /translation="MKKNKLVNKENYSILETLPEDPLFENKSTLEIDLNQFDLFNRIAN
                     ETVEELIIKEVNDPNDRSDKSNGVNLNAKVYVEKEKKTSLKKDFVITFVDNLEALAKLN
                     LKPNEFRIIVEIVKVMEYGNLINLSQSTIAKNLNLAKSNVSYYFKNLKKKNILVEKDGH
                     VFMNSNIFSKGLAHRLDEEKRKNLKSAQVEDDNFKNSF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1215..2387)
                     /gene="mobV"
                     /locus_tag="HV345_RS26190"
                     /old_locus_tag="HV345_26210"
     CDS             complement(1215..2387)
                     /gene="mobV"
                     /locus_tag="HV345_RS26190"
                     /old_locus_tag="HV345_26210"
                     /inference="COORDINATES: protein motif:HMM:NF013258.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_181636742.1"
                     /translation="MAYAILRVEKTKNTSIAGKNSHNMRLRKTHNADPNLKSQNRILIG
                     SGDLRTDINARFQATNVKARNSTSVICNELILTASPEFFENNKKLEDWVKVQMEYLQNE
                     FGENAINAVLHLDEQTPHIHAFITGIEYKNGEYKLNNKSYMKKYETMQDIYFKYNKPLG
                     LIRGVKKEVSNAKYKEVKEFYSDIKNIKNKTNLEIENNKIEKIRVVETEEKKKLFRDPE
                     VVPKHYTGAEVNRYIRKALKPYKNERKPLIARLNGFKSRLEHSEAELSDLRQNFNRRVQ
                     ERVQQEQKLAVAKATAEQEQIIQNKNRKIKDMELEIIEYKRQLKENASISQRYEKYKIN
                     SDVLDLIQEHKPAEFKQLFNAAIAAADAEYSQKQQNNNYTPQNKPKALKL"
                     /name="mobV"
     Typing          complement(1216..1412)
                     /name="Col(IMGS31)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.99"
                     /coverage="100.0"
                     /evalue="1.05e-95"
     Typing          complement(1219..2387)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.667"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaacaaaa gtcaacggat caatccctcg caaggggaaa ttaaaattcc cccttactca
       61 cgatttccaa aaaaaagaat gagcctataa cgctgagcaa gtcaaaattt taattttggc
      121 ttgtgaaggg ttgaccaagc gaagcgcggt agggaaatct gcgcagattg cttatgtatt
      181 gcccggaacg ggaaacgtct gttgtagcgg tagcgaaaac acatctcccg gaagggggtt
      241 ttcttttgcg tagcctggta agttctgctc gatctggagg tttgcaccgt ttactctctt
      301 actttcttat tgttttaaat cttacatacc cctccagccc ttgctattac tgacttaaat
      361 caaaaaaaag ttatagattc ctataaccta aaagttatag atttctataa ccccagttat
      421 agattcctat aaccccccta agttggtcat tcgaccaact tcttataact aagttataaa
      481 aagttgtaat catgtattga ctagttgtat attttgttta taactgagtt ataaatactt
      541 ataacttaat tattaatggg gttttaatat gaaaaaaaat aaattagtaa ataaagaaaa
      601 ttactcaata ttagagactt tgccggaaga tccattattt gaaaataaat cgactttaga
      661 aattgattta aatcaattcg atttatttaa tagaattgca aacgaaactg tagaagaact
      721 tataataaaa gaagttaacg atcctaatga ccgaagcgat aaaagcaatg gtgttaattt
      781 aaatgcaaaa gtttatgtag aaaaagaaaa aaagacttca ttaaaaaaag attttgttat
      841 tacatttgta gataatcttg aggctttagc aaaattaaat ttaaaaccta atgagtttag
      901 aattatcgtc gagattgtaa aggttatgga atacggaaat ctaattaacc tttcacaatc
      961 tacaattgcg aaaaatttaa atcttgcaaa atcaaatgta agttattatt ttaaaaacct
     1021 taaaaagaaa aatatattag tagaaaaaga cggacacgtc tttatgaata gtaatatttt
     1081 ttctaaagga ttagcccatc gtttggacga agaaaaaaga aaaaatttga aatccgcaca
     1141 agtcgaagac gataatttta aaaactcatt ttaaaaccca acgggaaatt tttcacagtt
     1201 tcccgttccg ggctttataa ttttaaagcc tttggcttat tctggggtgt gtagttatta
     1261 ttttgctgtt tctgtgaata ttcggcatct gctgctgcaa tagcagcatt gaagagttgt
     1321 ttaaattctg ccggtttatg ctcttgtatt agatctaaaa catcactgtt gattttatat
     1381 ttttcatatc tctgagagat tgaagcattt tcctttaact gccttttgta ttctattatt
     1441 tccaactcca tatcttttat ttttctgttt ttattttgaa tgatttgttc ttgctcagcc
     1501 gtggctttgg ccactgctaa cttttgctct tgttgtaccc gttcctggac tcttctgttg
     1561 aagttctgac ggagatcaga caactcagcc tctgaatgct ctaaacggct cttaaacccg
     1621 tttagacgag ctataagggg ctttcgttcg ttcttgtaag gttttagagc ctttcgtatg
     1681 taacgattaa cctcagctcc tgtgtagtgc ttaggcacta cctccggatc tctaaataat
     1741 tttttctttt cttctgtttc aacaactcta attttttcaa ttttattatt ttctatttct
     1801 aagttggttt tatttttaat gtttttaatg tcgctgtaaa attccttaac ttctttatat
     1861 tttgcattag aaacttcttt tttaacccca cgaattaaac ccaatggttt gttatattta
     1921 aaataaatat cctgcattgt ttcgtatttt ttcatataag atttattgtt taatttatat
     1981 tccccgtttt tatattcgat tccagttata aaagcgtgaa tatgtggggt ttgctcgtcc
     2041 aagtgtaaaa ctgcattaat tgcattttct ccaaattcat tttgcaaata ttccatttga
     2101 acttttaccc aatcctctaa ttttttgtta ttttcaaaaa attcagggga agcagttaaa
     2161 attaattcat tacaaataac agaagtagaa ttacgagctt taacattggt tgcttgaaac
     2221 cttgcattaa tatcagtcct taaatcacca gaaccgatta aaattcggtt ttgggattta
     2281 aggttaggat ctgcattatg tgttttcctc aatcgcatat tgtgagaatt tttcccggcg
     2341 attgaagtat tttttgtttt ttccactctt aaaattgcat aagccatatt cgaaaacctc
     2401 ccgttaaaag cagtaaggtt tttttctttt tggcccctgc caggctcaca ccgagatttc
     2461 tcggtatagt gagtatacct ttctgcaaaa ttgaaaatct ataaatacat ctacaataaa
//
