LOCUS       NZ_CP056206             3078 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Citrobacter sp. RHBSTW-00986 plasmid pRHBSTW-00986_7, complete
            sequence.
ACCESSION   NZ_CP056206
VERSION     NZ_CP056206.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148762
            Assembly: GCF_013783065.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00986
  ORGANISM  Citrobacter sp. RHBSTW-00986
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Shaw,L.P.
  TITLE     REHAB project genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00986
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.3024685258756x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/05/2023 04:44:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,156
            Genes (coding)                    :: 5,003
            CDSs (with protein)               :: 5,003
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 55 of 153
            Pseudo Genes (incomplete)         :: 101 of 153
            Pseudo Genes (internal stop)      :: 29 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            Pseudo Genes (short protein)      :: 1 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056206.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Citrobacter sp. RHBSTW-00986"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00986"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742675"
                     /plasmid="pRHBSTW-00986_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2974..3078,1..237))
                     /locus_tag="HV345_RS26165"
                     /old_locus_tag="HV345_26185"
     CDS             complement(join(2974..3078,1..237))
                     /locus_tag="HV345_RS26165"
                     /old_locus_tag="HV345_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001973.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_049592808.1"
                     /translation="MFTLQKPMHPGEFIREVYLEPLELQIGTFAEKLGVDTSTVSRLVN
                     QKADLSYEMALRLSKALGRSADSWMKMQIAYGLYRAQDKVNLEEVEVVYRPRNLDAPEK
                     QVHEFYENI"
                     /name="HigA family addiction module antitoxin"
     gene            508..783
                     /locus_tag="HV345_RS26170"
                     /old_locus_tag="HV345_26190"
     CDS             508..783
                     /locus_tag="HV345_RS26170"
                     /old_locus_tag="HV345_26190"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094544860.1"
                     /translation="MIKNFKSKKLKQFNSGDTSKVAQAHKKKIEIILNALDTATSINDL
                     DLPGLDLHSYAEYTPTRWSLKVSANYRIFFEWDGANVRNVDYDDPH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2074..2301
                     /locus_tag="HV345_RS26175"
                     /old_locus_tag="HV345_26195"
     CDS             2074..2301
                     /locus_tag="HV345_RS26175"
                     /old_locus_tag="HV345_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(2349..2537)
                     /locus_tag="HV345_RS26180"
                     /old_locus_tag="HV345_26200"
     CDS             complement(2349..2537)
                     /locus_tag="HV345_RS26180"
                     /old_locus_tag="HV345_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1051..1601)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.203"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 atataaacca tatgctattt gcattttcat ccaagagtca gcagaacgcc ctaaggcttt
       61 tgataggcgt aatgccattt cataagaaag gtcagctttt tggtttacta accggctaac
      121 ggttgatgta tcaaccccaa gcttttctgc aaacgttcct atttgaagct ctaacggctc
      181 taaatatact tcgcggataa actcgccggg gtgcataggt ttttgcaatg taaacatgca
      241 acctcctaaa tactctgatt ttgtgccttg cgcaacgcgc aaacctattg acataagcaa
      301 tccatttgtg ataaaatgga ttagttaagg gtaatttttt tcttaacgtt aacagtttgg
      361 ttcttctgtt ttgttcttgt agtgactatc tggcagttct aaagcttcgc aggcgtagaa
      421 tactacctat aaatagaaca cagtcaagaa actttactaa acaagaagaa cttctaaaac
      481 ataaaaattt tcatatggag aagttcaatg attaaaaatt ttaaatccaa aaaactaaaa
      541 caatttaaca gtggtgatac aagtaaggta gcacaagcgc ataaaaagaa aatagagatt
      601 atcttaaacg ctttagatac cgctacttca ataaatgatc ttgaccttcc tggtttggat
      661 cttcattcgt atgctgaata taccccaacc cgctggtcat taaaggtgtc ggcgaactat
      721 cgtattttct ttgagtggga tggtgccaac gtccgtaatg tggattacga cgatccacat
      781 taatgtcagt taaatcaacg acttaggtca tgggcttgaa tcactaaagt gattcaagcc
      841 catgaggcta ttatatggtt tttttttatt aagtcaatgg cttggtaaag ataatttctt
      901 gagattgagt gggacgcaga gtatctttaa tcctgaaacg aaaaaaccac ctggggaggt
      961 ggtttttcga aggttcagca agttggggaa cttctgaacc gtggtaacag ggtgtacaag
     1021 accgctgcca ccaaatacgt ccttccagtt tagccgtagt taggctgata cttcaagaac
     1081 tctgcattag taatctcttg tacaccctct taccagtggc cgctgccagt ggcgataagt
     1141 cgtgcctttc cgggttggac tcaagacgat agttaccgga aaaggcgcag cggtcggact
     1201 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     1261 tcaggcgtgg aatgagataa acgcggccat aacagcggag tgacaccggt aaaccgaaag
     1321 gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt
     1381 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     1441 cgaagcctat ggaaaaacgg cttcgccgtg gccttcttgc ttccctgtta agcttcttcc
     1501 tggcatcttc caggaaattt ccgccccgcc agtaagccgg taccgctcgc cgcagccgaa
     1561 cgaccgagcg gagcgagtca gtgagcgagg aagcggaata tctccgggct tatctttggc
     1621 accgtacgcg ccatagctgc attttttaat acgatgcagg atagggctaa cgccgcaaaa
     1681 tgacgtcatc cgacatcatt ccgccgcgtt agcccagtga cctacagtcg gctctggggt
     1741 gtcggggctg tgccctgacc aggcgttttg gagtatcgcc accgtggtgg cggtgctaca
     1801 aaacatatcc tgtcccttcc ttggcatctc tgaagtttag cctgtataca ctccgctagc
     1861 gctacgtgac tggttcaggg ctgcgccccg acacccgcta aaagcactga cgcgcctgcg
     1921 gcttgcccaa ccccgcgccg tccgggaaag aatttcccga tcgacccgac gtttctgttt
     1981 acctgtgatg ttaacgtttg gatctttttc ccgggtttac ctattttctt ctaatgaagc
     2041 gttcacagct gacgttaata gaggggtgct ttcatgtttg gtttttcacc aggagaactt
     2101 atgaaccttt gccccgatga acgtctgctc tttgtgcgga tgatttccgc tatgcttcgc
     2161 cgttcgggcg gtgatgcggg tgctgttatg tttgaggcgt atcgccatat tgtgtccgat
     2221 accaatcaag ctcgtcgttc ctacatgctc gacctgctcg aaagcgtcag gcatgactat
     2281 gtacacggtg gatatacata gttgccctgg atacgctttt cgtctaagtg agcgttcttt
     2341 tcgtctcttt attcgccgtc ctgtataccg aagcgtgcgc tcagcttctg ataaagcgct
     2401 tctgcctgtt cgtgcattcg ctcacagtcg gctgcctcgt catcaagatc gagcacgtcg
     2461 agtttttcga gcagcagcag cgactgcgcc cggataaatt ttgccatgtt cagtgcggct
     2521 gattcctgtt ttgtcatacg gctttttccc ggttttatgg tgatcgcaga ctgaaatctt
     2581 acttcattca cttgagtgca tagccatgca ggtacgcaga aatcatcagg ggcgtttttt
     2641 ctcgatatgg gcacagaaag gtgtatgttt caggccggag tcctcagaaa cgccctgagg
     2701 gcgcctgagg tggtgcgggc aacggatgtt atggtaaatt ctctaggtaa gcatagaagg
     2761 gggctggcta tgaagtttgg ggttagtgaa gaggatatac agatcactat caatggttga
     2821 tacaggctca acacccgctg tttaagcatc ttcaggaata acgatgctgc cgcctctgcg
     2881 agtgcaagtt aggtcttcac ataaatacac aggtgtagag acaaaaagcc cctatggtta
     2941 ggagcttttc tttacctaaa tgaaatatcg tttttagata ttttcataaa attcatggac
     3001 ctgtttttca ggagcatcca agttccgtgg gcggtaaacc acctcaactt cttcaagatt
     3061 tactttatct tgagcacg
//
