LOCUS       NZ_CP056205             4183 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Citrobacter sp. RHBSTW-00986 plasmid pRHBSTW-00986_6, complete
            sequence.
ACCESSION   NZ_CP056205
VERSION     NZ_CP056205.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148762
            Assembly: GCF_013783065.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00986
  ORGANISM  Citrobacter sp. RHBSTW-00986
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Shaw,L.P.
  TITLE     REHAB project genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00986
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.3024685258756x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/05/2023 04:44:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,156
            Genes (coding)                    :: 5,003
            CDSs (with protein)               :: 5,003
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 55 of 153
            Pseudo Genes (incomplete)         :: 101 of 153
            Pseudo Genes (internal stop)      :: 29 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            Pseudo Genes (short protein)      :: 1 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056205.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Citrobacter sp. RHBSTW-00986"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00986"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742675"
                     /plasmid="pRHBSTW-00986_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3832..4183,1..842)
                     /gene="mobQ"
                     /locus_tag="HV345_RS26140"
                     /old_locus_tag="HV345_26160"
     CDS             join(3832..4183,1..842)
                     /gene="mobQ"
                     /locus_tag="HV345_RS26140"
                     /old_locus_tag="HV345_26160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_000190114.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYVDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            1380..2237
                     /locus_tag="HV345_RS26145"
                     /old_locus_tag="HV345_26165"
     CDS             1380..2237
                     /locus_tag="HV345_RS26145"
                     /old_locus_tag="HV345_26165"
                     /inference="COORDINATES: protein motif:HMM:NF015076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000586611.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEKVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQEKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPLNPLQYKELKATARSIARWTWKNFDSHTFSLIQSSRGAKNKNKPKKSNVKEMLGFI
                     GGEK"
                     /name="replication initiation protein"
     gene            2234..2503
                     /locus_tag="HV345_RS26150"
                     /old_locus_tag="HV345_26170"
     CDS             2234..2503
                     /locus_tag="HV345_RS26150"
                     /old_locus_tag="HV345_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_021197950.1"
                     /translation="MRRSTIRKGLTQKEMAERFNISVKTVKKYTSEPREQYEQTAADRR
                     RQAYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPKNSL"
                     /name="response regulator transcription factor"
     gene            complement(2749..3018)
                     /locus_tag="HV345_RS26155"
                     /old_locus_tag="HV345_26175"
     CDS             complement(2749..3018)
                     /locus_tag="HV345_RS26155"
                     /old_locus_tag="HV345_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_000047246.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LDKKPEEINKALILGLLSKINELDEEEKRDFTRIGMDILQQWKS"
                     /name="conjugal transfer protein TraD"
     gene            complement(3020..3409)
                     /locus_tag="HV345_RS26160"
                     /old_locus_tag="HV345_26180"
     CDS             complement(3020..3409)
                     /locus_tag="HV345_RS26160"
                     /old_locus_tag="HV345_26180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223168448.1"
                     /translation="MQYRGLEFEEQEFNDCLNILHKEVVSFLNKNNMALDFFQYPVEDI
                     CRIIFRDKIDLRTAVILDFGITTYDLSPLGDHDPFVWRLLEWPLYDYENFQFVIVQKLA
                     SYELDKLNEIIQVEDNKDNKDKRRL"
                     /name="hypothetical protein"
     gene            complement(3426..3590)
                     /locus_tag="HV345_RS26630"
     CDS             complement(3426..3590)
                     /locus_tag="HV345_RS26630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162865999.1"
                     /translation="MEKETLGPDIQVNWCDHCEKNTRHIFRFTEEYSKEEGPITEVECT
                     KCGNTFTIL"
                     /name="hypothetical protein"
     Typing          4..659
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="2.97e-133"
     Typing          3833..4182
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.145"
                     /coverage="85.0"
                     /evalue="3.16e-70"
ORIGIN
        1 gaatgattgc agacatagca tttcatgatc ttgatagtca taaccctcat gctcatgtaa
       61 tgctaacttt aagaactgtt agcccaaatg gatttgggaa caaacaaaga gaatggaata
      121 atagagaaaa tattgaagcc tggcgagtta actgggaaat aatagcaaat gacagattaa
      181 aaaaatatgg tcataagtcc cgtattgatt cccgttcatt aaaagatcaa aaagaagagg
      241 cagaaagaaa ggctgttttt gctaaaactc cgaaagagaa agccaagtgg atttcaaaaa
      301 ctattgaact ggacagaacg ccaatgacca ggatacacag gcaccaatgg cgaaaaggtc
      361 aaaaattaag agctatggag caaaaagaaa aagcattatt attaaaaata gctaatgcaa
      421 catacttaca gcaaaccaaa ccacctcagc cagaggaaac tatgaaaata gagaaaaagc
      481 ctcttgcaag gtcaatcatt gataatttaa aggcattata caaaaagacc cagcatatat
      541 ttaaatcaaa acctaatgta aataaaaata aaaaagaaga aaaagaagat cctcaaaatt
      601 tcgttagtag ttatgtaata aatgaatttg gcgaacatat attaaagaca gagttagaaa
      661 caaaacctaa aacaggcaca ggaaccggaa ccggtggaaa taatggaggc ggaaaaatgg
      721 gttcaggctc tggaatgtct cctatgaacc aaccacaacc aaaaattgaa gagcctgagc
      781 cggaaccaat gccggaattc gaagatcagc aacctaagag gaagaagaaa tttggaatgt
      841 agtatatagt ggtatatatt cctatatagt agtatcactt aatacaactt aatattattt
      901 aatactatac tataatatac tacattttta gagttgtgat cctcgccggt tctatttccc
      961 ctttatgtat tgccacagct tcattaactg acttcataag cattttaaaa aatttactat
     1021 gcttatttct tttccgtttt ttctgcactt tctttttttt catatattca ccaaaaaaaa
     1081 tcgaatcgcc aatttttctt cgaacaatac tcttttttct ttttttattg tttttctatc
     1141 ttcggtaatg cttcgcacca tagatctgat agtaatgctt tgctgccgta tgtaaagaac
     1201 tgccccaact tcgttgggct gttatctgat atggttaagc tccccacttg acaaattttt
     1261 aagatcggtt agaactggta caggattgaa aaacctataa aaataaaaac cccaccctct
     1321 gcgattttgt tttggcgaca gaaaacagag agtagggcca gataaaaagg actgaaaaaa
     1381 tgataataca agaagaacat aacatcgatt tttttaaaaa tcaaatgcct aacaagcctt
     1441 attgtacaaa taacctcgat atgggattaa gcataaggaa caaggcaaaa gccttagaaa
     1501 tgctgtattt acaggcaaat cagccagcaa tccaaacttg cttactcttt gatttagata
     1561 agaaaaactc attctataca tttgagaagg tgggcttacc catacctcac tttattacaa
     1621 aaacaccaaa gacaggtcgt tgccattacg ggtacatgct taaagccggt gtttgtaaga
     1681 cacaacaagc aaggttgaag ccgttgaagt atgccgctgc cgttgaaatg gctatggcga
     1741 aaaaactcaa agcagatctg gggtttgctg gattgattac aaaaaatcca ctcaatgatc
     1801 actggtcgcc gttctggtca ggtgctgacc tttacgacct ggattattta gctgattttg
     1861 tggacttaga aaaacctcaa atacaagaga aaaaaagcga ggcttatggt ttaggtcgca
     1921 atgttaattt gtttgaagat ctacgacaat atgcttacag aacggtttta aatttcaaaa
     1981 aaatatctac tttcgagaaa ttcgaaaacg aactattatt aaaagcacag ggtttaaata
     2041 ctttttgcaa tccactaaat ccgttacaat acaaagagtt aaaggcaaca gcaagaagca
     2101 tagcccgttg gacctggaaa aattttgata gccatacatt ttcactaata caatcttcaa
     2161 gaggtgcgaa aaataaaaac aaacccaaga agagtaatgt taaagaaatg cttggtttta
     2221 taggaggtga aaaatgagaa gaagcacaat aagaaaaggt ttaactcaaa aagaaatggc
     2281 tgaaagattt aatatatctg ttaaaacggt aaaaaaatat acatccgaac cgcgtgaaca
     2341 atacgaacaa acagcggctg atcgtcgtcg tcaggcttac gaactacgaa gccaggggct
     2401 gaaatggcaa caggtagccg acaaaatggg ctgttcgtat cacggagccg ttgccctgta
     2461 tcgccgttat gttgccttag atatgcctaa aaacagcctg taacgacttc taagcagttt
     2521 ttccccctaa cctaaccaaa ccctccaccc ctggggggat ttttccccct ttccccgtat
     2581 ccgggcttgt gtctggatag gtgagggaca ctctggtacc aacaaatcaa caaacccacc
     2641 cagccaccgg aggccctctg gcaaccttaa aacctccagc cgttggctgg caacccttca
     2701 cagcaaaaaa ccagcccaaa aagcggctgg ttcttcactg ttcacggttt acgatttcca
     2761 ttgttgcaat atatccattc cgattcgtgt aaaatctctt ttctcttctt cgtctaactc
     2821 attgatttta cttaacaagc caagaattaa agccttgtta atttcctctg gttttttatc
     2881 taacacctta gccacttctg cacccaggat tattttttgt cttgtttctt cccgtctgtc
     2941 ctgccttgct ttttttgttc ttagtgcttg taatttattt tgtagatctg cgatctgctg
     3001 tgctgttgat ttgctcatat tacaacctcc ttttatcttt attatctttg ttatcttcta
     3061 cttggattat ttcatttaat ttatctaatt cataagaagc cagtttttga acaatcacaa
     3121 actggaaatt ctcataatca tataatggcc attcaagcaa ccgccaaaca aaaggatcgt
     3181 gatctcccaa agggcttaaa tcatatgttg taattccaaa atctaaaata acagcagttc
     3241 tcagatctat tttatctcga aaaattattc tacatatatc ctcaactgga tactggaaaa
     3301 aatccagagc catattattt ttatttaaaa aagacactac ctctttatgt aaaatgttta
     3361 aacagtcgtt aaactcttgt tcttcaaact ctaaacctct atattgcata ttaaaaacct
     3421 ccacactata agatagtgaa tgtattacca catttagtac attcaacctc tgtaattggt
     3481 ccctcttctt ttgaatactc ctcagtaaat ctaaatatat ggcgagtgtt tttttcacaa
     3541 tgatcacacc aattgacttg tatatcaggg ccgagcgttt ctttttccat ataacctcca
     3601 ttattttatt ttgttattta ttacctccat tatttacttt agtcaaatat attttagaat
     3661 aaaataaata atatttaata ttgggcgaag cctggggttc attacttatt attttgaggt
     3721 gaagccgaaa aaataatatt agtaatgccc acttacacgt cgcaagcgac gtatgggccc
     3781 tgcggggcta aaaaccaaaa ttcaaaagac aaaaaaagta aggggtagac tatggcgaca
     3841 tttcatatca gctttaaaaa attacgaaga tcagaaggta aatcttcggt ttatttatct
     3901 gcatatcaaa accgagaaaa gacaaaagat aatcgaaccg gggcaacctg ggattattca
     3961 aaaaaagagg gatttttcgg ctctgctatc ctctcccctg ccggcacacc tgccgaactg
     4021 gtgaaggact caggcaccct ctggaatgcc gtagaggctg gcgagaagcg aaaggatgcc
     4081 gaattatgtc gctatgtgga catagccatc cccaaggagc tggacgacgg ccagaagaag
     4141 cagatcgtcc tcgattactg ccaagaaaat ttcgtggatt atg
//
