LOCUS       NZ_CP056204             4667 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Citrobacter sp. RHBSTW-00986 plasmid pRHBSTW-00986_5, complete
            sequence.
ACCESSION   NZ_CP056204
VERSION     NZ_CP056204.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148762
            Assembly: GCF_013783065.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00986
  ORGANISM  Citrobacter sp. RHBSTW-00986
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Shaw,L.P.
  TITLE     REHAB project genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00986
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.3024685258756x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/05/2023 04:44:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,156
            Genes (coding)                    :: 5,003
            CDSs (with protein)               :: 5,003
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 55 of 153
            Pseudo Genes (incomplete)         :: 101 of 153
            Pseudo Genes (internal stop)      :: 29 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            Pseudo Genes (short protein)      :: 1 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056204.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Citrobacter sp. RHBSTW-00986"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00986"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742675"
                     /plasmid="pRHBSTW-00986_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            41..1597
                     /locus_tag="HV345_RS26110"
                     /old_locus_tag="HV345_26130"
     CDS             41..1597
                     /locus_tag="HV345_RS26110"
                     /old_locus_tag="HV345_26130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_045326453.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            2657..2980
                     /locus_tag="HV345_RS26115"
                     /old_locus_tag="HV345_26135"
     CDS             2657..2980
                     /locus_tag="HV345_RS26115"
                     /old_locus_tag="HV345_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3189..>3623
                     /locus_tag="HV345_RS26625"
                     /pseudo=""
     CDS             3189..>3623
                     /locus_tag="HV345_RS26625"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3658..4143
                     /locus_tag="HV345_RS26125"
                     /old_locus_tag="HV345_26145"
     CDS             3658..4143
                     /locus_tag="HV345_RS26125"
                     /old_locus_tag="HV345_26145"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            4147..4362
                     /locus_tag="HV345_RS26130"
                     /old_locus_tag="HV345_26150"
     CDS             4147..4362
                     /locus_tag="HV345_RS26130"
                     /old_locus_tag="HV345_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4494..4655
                     /locus_tag="HV345_RS26135"
                     /old_locus_tag="HV345_26155"
     CDS             4494..4655
                     /locus_tag="HV345_RS26135"
                     /old_locus_tag="HV345_26155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_154393737.1"
                     /translation="MDICAESRSHSPDCIAMQPGEIYLAQNARFSGEFAAGFTGLTPHL
                     FRTRPGRC"
                     /name="hypothetical protein"
     Typing          2372..2544
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(1895..2447)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2658..2974
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          2971..4466
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggtaaatgc ggcatttttt attatatgga gtgtattttt atgagaatta aaaagcctca
       61 ccttcgtccg atagtcataa ccttgtccgt tggtgggatt ttgttaactt ctatttttct
      121 gattgccgtt attatggttt ttcagcgcga aaatatagaa aaagatttgc ttgatgctaa
      181 ttcatcgtac gctatgaaaa tgtcagatgt aatgagtggt tatattgaaa tggctcaagg
      241 tgaattagca tatggagcaa aaaaaataaa atcaactaat gatattgact cacttaaaga
      301 tgaggcggat aggttaagat tgcagtctgg tatgtttaat tcagtttttg ttgttagtaa
      361 tgatgctgtt gttctggcga cttcgccaga atctcttgat ttagttggta cccgcttgaa
      421 ttcaagtgcc agcaaattgg cgattgagga aaagaaacca ttcatatctt caccgttcaa
      481 atcagttact gggaatttga ttattttgtt gtcacatcct atttatgata agaatggcaa
      541 ttacataggg catgtcggtg ggtctatata tctaaaaaaa catagtttat ttagcgatgt
      601 tttgagcagg cattttttca agagcgacac tgaaattagc attgttagtg atgatggaac
      661 tgtgatcttt aataacgata acagtgctgt tggtcggccc atggttatgt ctgatgattt
      721 gaaatcaaag ttaacggagt ctgatagagg tgagggggaa tttttttttt cggggaatag
      781 atatcttctg ggttatgctc atatgaaaaa tactgcttgg aatgtgttgg tttatagcca
      841 tgctgacagc gcgactacta ttcttattac ttatgctaaa agtgtacttg ttctgctaat
      901 tgtaataatt attacatttt cacttctttc atatttcatt gcctcccgta tttcacttcc
      961 tttagaaaag cttgctatat ctaccaatgc taaagatata ggggcttcgt tagtttatat
     1021 taaagctatc aatgcttggt atgccgaagc tgatcgactt aaaaaggcac tttttacgca
     1081 tgttaaggta atgatggaaa gggttgattc tttaaatgag gtggcgctta aagatcccct
     1141 taccggcgtg aacaatagat tggggttttc aactaaaata cagaataaca agtatggggc
     1201 aggagcttca gttattgcta ttgatgttga tttttttaaa aaaataaatg atagtttcgg
     1261 gcatggggtc ggagatgagg tgcttgtttc tttggctcag attattaagt cttgttgccg
     1321 tagtgaagat attatttgtc ggtttggtgg agaagagttt gttgtttttc tgccgaatac
     1381 atctgtggta acagcggagt gtgttgcgga gcgtattagg agtgttattg aacgtactgt
     1441 tttccctaat aacttgcgtg tcaccgtatc tgctggtgtg gcaacacaag atgatcctct
     1501 tggtgggatc gattttttgc tgaaaaatgc tgacgatgct ctttatcaag ctaagagaga
     1561 ggggcgtaat aaagtgattg tatatagcgc tatttgagtt tttttgatga cgctttacgc
     1621 gctagtgtgc cgtttttttt tgtggggagg ggggttcaag aagcggatcc tcttgttatc
     1681 ccttcgatgt cgtcgtaatc tcatgccctg caaacaaaaa aaccaccgct accaacggtg
     1741 gttcgtttgc cggaatcaag agctaccaac tctttcttcc gaaggtaact ggctttcacg
     1801 gagcgcaggt accaaaactg tccttccagt gtagccgcag tcaggccacc acttcaagaa
     1861 ctctgtagaa cactctacta ctccgctccg tgaatcctct tactaatggc taccgccact
     1921 ggcgattttt cgtgtctgtt ccgggttgga ctcaagatga tagttaccgg gacaacgtgg
     1981 tagtcgggct gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcccg
     2041 gaaccgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggat tgacaccggt
     2101 aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta
     2161 tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt
     2221 gtcagggggg cggagcctat ggaaaaacgg cttcgccgtg gccttatcgc ttccctgtta
     2281 agcttcttcc tggcatctcc caggaaattt ccgccccgcc tgtaagccgg taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaata tatcttgtat
     2401 cacatattct gctgacgctc cgctgctgca tttttctcct gccacatgaa gcatttcact
     2461 gacactcgca tccgtgccaa catagtaagc cagtatacac tccgctagcg ctacgtgact
     2521 ggttcagggc tgcgccccga aacccgccaa aagcctgacg cgcctgcggc ttgtccaacc
     2581 ccgcgccgac cgggaaaggc tttcccgccc gtcccggcgt tatcaggagg ccggtttgtc
     2641 agagaaacgg aacaagatgc tcaccatgtg ggtgacggag gacgagcacc ggcggctgct
     2701 tgagcgctgc gacggcaggc agctggcagc gtggatgcgg cagacctgcc tggacgaaaa
     2761 gccggcacgt tccggaaagc ttccctcaat ctcgccggcg ctgctgcgcc agcttgccgg
     2821 catgggcaat aacctgaacc agatagcccg ccgtgttaac gccggcggcg gtaccggcca
     2881 tgacagggtg cagattgttg cggcgctgat ggccatcgat gccggacttg agcggctgcg
     2941 gcacgccgtt cttgagaaag gaacggacga tgatcgttaa atttcatcca cgcggtcgcg
     3001 gcggtggcgc cgggccggtg gactatctgc tgggtaaaga ccggcagcgt gaaggcgcca
     3061 ccgtcctgca ggggaagccg gaagaagtcc gggagctgat tgacgcgtcg ccttacgtta
     3121 agaaatacac ctccggggta ctgtcgtttg cggaagcgga tttaccctcc ggccagcggg
     3181 aaaagctgat ggcaagcttt gagcgggttc tgatgcccgg acttgataaa gaccagtaca
     3241 gcatcctgtg ggtggagcat cgtgacaagg ggcggcttga gctgaatttt ctgatcccga
     3301 acacggagct gctgaccggc agacggctcc agccgtatta cgatcgcgcc gaccgtccac
     3361 gcatcgatgc ctggcagacc atcgtgaacg gcagactggg gctgcacgac ccgaacgcac
     3421 cggagaaccg gcgggcactg gtcacaccgt ctgcactgcc taaagcgaag caggaggctg
     3481 cagaagcgat tacacgcggt ctgcttgctc ttgcctcatc cggggagctg aaaacccgtc
     3541 aggatgtcac tgaggcgctg gaaagcgcag gttttgaggt ggtccgcacc acaaaaagca
     3601 gtatcagcat tgccgacccg gacggggggc gaaacatccg actgaaggga gccatttatg
     3661 aacagtcttt taacgctggc gaaggactta gagcagaaat cgaaagcgca gcagcagagt
     3721 accggcgaga tgctgaaagc cgcattcagc gagcacgaga aatctgtaaa agcggaactg
     3781 aacgaaagcg cgaagagaat cagcgccgcc atcctcgacc acgaccggaa actgtcctca
     3841 gccatgagcc agcgcacgaa aggcatggtg cgcatggtca gccagacgtg gctgaccatc
     3901 gtcctggtct ccgcgctgct gatcgcctcg agcgcgggca ttctgtggtg gcaggggcag
     3961 cagatactcg acaactacac gaccatccgg gagcagaaga gcacgcaggc catgctgtca
     4021 gagaggaaca gcggcgtgca gctctcgacc tgcggcgagc agagacgccg ctgcgtgaag
     4081 gtgaacccgg aagcgggacg gttcggagag gattcgagct ggatgatact ggcggggaaa
     4141 tagcacatga cggagctgga aaaacagttg ctgagcgcat tagagcagct acagcaggac
     4201 tactcgaaaa ggctggacga gtgggagagc gccttcgcgg aatggcggac gatgtctggt
     4261 cttatgcaac gggagaacgc agcgctgagc gagcgcgtca cgggcttgag caggcaggtg
     4321 cagagtttga gcgagcagct gcgccggttg tcgagcgact gaatgccgtc gagctgcacc
     4381 gggagcagga gcgcgtggtg cagcatcaga aagcgctgga gctggagcga tctcagcgac
     4441 agcggacgta taacagccct tcactgtgat aacctgataa aacggggctt tcggtggaca
     4501 tctgcgctga aagccggagc cattcacccg actgcatagc catgcagccg ggtgaaattt
     4561 atctggcgca gaatgcgcgg tttagcggag agtttgctgc cggttttacc gggctgacgc
     4621 cgcatttgtt ccgaacccgt ccggggcggt gctgataagg gatgtta
//
