LOCUS       NZ_CP056203            13256 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Citrobacter sp. RHBSTW-00986 plasmid pRHBSTW-00986_4, complete
            sequence.
ACCESSION   NZ_CP056203
VERSION     NZ_CP056203.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148762
            Assembly: GCF_013783065.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter sp. RHBSTW-00986
  ORGANISM  Citrobacter sp. RHBSTW-00986
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 13256)
  AUTHORS   Shaw,L.P.
  TITLE     REHAB project genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13256)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00986
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.3024685258756x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/05/2023 04:44:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,272
            CDSs (total)                      :: 5,156
            Genes (coding)                    :: 5,003
            CDSs (with protein)               :: 5,003
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 55 of 153
            Pseudo Genes (incomplete)         :: 101 of 153
            Pseudo Genes (internal stop)      :: 29 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            Pseudo Genes (short protein)      :: 1 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056203.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13256
                     /organism="Citrobacter sp. RHBSTW-00986"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00986"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742675"
                     /plasmid="pRHBSTW-00986_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(12671..13256,1..200)
                     /locus_tag="HV345_RS26020"
                     /old_locus_tag="HV345_26040"
     CDS             join(12671..13256,1..200)
                     /locus_tag="HV345_RS26020"
                     /old_locus_tag="HV345_26040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="WP_181636740.1"
                     /translation="MRIYSYLTRDGSEKVNMQIFRALEAVSETLSIPLDVTIPVEERGE
                     VLERLVSGAATGDIIIITEEQPLYDMTKQRAELIRSIIEEKGIYLLRPFSDGYEPGYTQ
                     DLSDVASLDESGRHGALMVSTSINLYGMWTYVKHEKIAVTPDSGVPLLPPAPSLAYVIR
                     AGKNLCKIGYSHNPKGRVSSITRSMTEAGAIGKKTLTLEGAFTFEKISAQSAELMAHLF
                     FSRKNSGLKGFDGATEFFNVDAKKACAFFEEAGGIRQPQ"
                     /name="GIY-YIG nuclease family protein"
     gene            305..496
                     /locus_tag="HV345_RS26025"
                     /old_locus_tag="HV345_26045"
     CDS             305..496
                     /locus_tag="HV345_RS26025"
                     /old_locus_tag="HV345_26045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022314.1"
                     /translation="MQSIQQTTPARYPGLSIENGEIRQHGKQRHCAANRKGQYQTTLRD
                     SAGRYQTVSILSLYAWEA"
                     /name="hypothetical protein"
     gene            493..690
                     /locus_tag="HV345_RS26030"
                     /old_locus_tag="HV345_26050"
     CDS             493..690
                     /locus_tag="HV345_RS26030"
                     /old_locus_tag="HV345_26050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_089503153.1"
                     /translation="MIMIPGRTQILKATADIAKALGTDTADVERHFLQHAVVLARLRQE
                     GKRPRIKAWMAENIGTPSAM"
                     /name="hypothetical protein"
     gene            791..1141
                     /locus_tag="HV345_RS26035"
                     /old_locus_tag="HV345_26055"
     CDS             791..1141
                     /locus_tag="HV345_RS26035"
                     /old_locus_tag="HV345_26055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181636741.1"
                     /translation="MRTKAIIAAVLLATAGTAAAAPVATTYICNDGTFIHLNEQNGRLT
                     ASWRGADIPVTKIETGKLTASLNGEQGSSTLQLVVGYNTTPNPIAMITTIVNGSMNSQS
                     CKPDTASKHIDL"
                     /name="hypothetical protein"
     gene            1161..1403
                     /locus_tag="HV345_RS26040"
                     /old_locus_tag="HV345_26060"
     CDS             1161..1403
                     /locus_tag="HV345_RS26040"
                     /old_locus_tag="HV345_26060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634175.1"
                     /translation="MAFENIERRFQRWKVIMVPGAKSYESWDEMVTGENFPEEARYKYK
                     THRTKAIDLTLKNPDYVHTMSENGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(2098..3669)
                     /locus_tag="HV345_RS26045"
                     /old_locus_tag="HV345_26065"
     CDS             complement(2098..3669)
                     /locus_tag="HV345_RS26045"
                     /old_locus_tag="HV345_26065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065945238.1"
                     /translation="MSQTDDNSGAALATVTPIAATPSPLSLKLSDALFSVLSVSVDWSG
                     DYRAQFELYGLNVKAINSAVCTAVWHAGKGRFLSVLNGSLTEFDKGDGMKLLEDSCGKF
                     WHRTDAFSARLTDLKIKTDDKVTKACIDMARAVRQAVAEFIMLRRQVAVVRLDVDMFAT
                     APRVELVGETVTFVRPHAPYPVANADSDVVADWLVHFPQCHEFLDALVAARFASSRKNA
                     YLFFRAQSDWGKGLLFGAGGVLSRLGATVELSEGELLNILSGANSGVTAGNFMGALALI
                     VNECTRVTKKHFRLEESLALTPKYLTTQCVNLYMKIFTSADPIPGLSDSDGIDPQVANR
                     FSMLDLQGDIKTRPLFLADKGRYVDSLTAFFAAELNARVASYQAKGFETARRDADHVLA
                     AFTTAHGLANAAGLITDAYDEIRADWVAFVHGRVADGHPDFLTFESKAGLVLRSPVGCW
                     GKFLDWYVDKSEPLRRARLMHDRDLIIGCAKKYRDVGGAAEGKGVLIPFVSGSMPDNVH
                     RFRYKN"
                     /name="hypothetical protein"
     gene            4184..4711
                     /locus_tag="HV345_RS26050"
                     /old_locus_tag="HV345_26070"
     CDS             4184..4711
                     /locus_tag="HV345_RS26050"
                     /old_locus_tag="HV345_26070"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_157914642.1"
                     /translation="MKRKASTFGLRLPPELLEQVNELAEKTNRTRTDVITDALRAYMGI
                     QEPQGESGNRLDLMVELLQDISNTLKHGVPQKAARRATAPRAEKQYGSTLDTQNEAQRP
                     AGAEISGHYDEAEVMATIRRMREEQRDRGFRYDNKAIAQALNEAGLLQSNGRPWNNDRI
                     NTVITRRMPDLK"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            complement(4771..5052)
                     /locus_tag="HV345_RS26055"
                     /old_locus_tag="HV345_26075"
     CDS             complement(4771..5052)
                     /locus_tag="HV345_RS26055"
                     /old_locus_tag="HV345_26075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249902.1"
                     /translation="MTDQTTTVADLALSQSAFLVSQAIVELDAMFGEGYAAAHPAVLAQ
                     FLQTAGVIYATQLQATAAFDLAQQLDRLIGLGLGVEAGLLQAAQGASH"
                     /name="hypothetical protein"
     gene            complement(5119..6258)
                     /locus_tag="HV345_RS26060"
                     /old_locus_tag="HV345_26080"
     CDS             complement(5119..6258)
                     /locus_tag="HV345_RS26060"
                     /old_locus_tag="HV345_26080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249901.1"
                     /translation="MVNERRDYLFDHAVFSAALARVDPDPSVMGWLAQARTLACWCELL
                     GMDPLETCVEWSARGVNAGKGDRAMLVSALRDLDELDRLAAAGAFLRSSGVAADVAAGR
                     IAVREPSEDELAARQAHLVALRERAAAAAEKLTEARIKATGRARMLLHRATKPGSAALY
                     WQAKERAACLPLPCPDDVKCSDWRTFLPVIGQVYWNSSLLVPLYGVNESLRLEQMSVEV
                     ICGRANPEAAGSLGEKRGLKNAPREGLFYPFDLDAVGSGAPVVVCEGFMSGLALSLLIG
                     GKLPVVCAMSVGNFGATVQSLNKFVMGGHPFVLVPDIGGQDLAIDQAIPNARVIDLSAV
                     PGAEGVDGYDPFDLLARHGVEMGRKYLRGLFREARDASL"
                     /name="hypothetical protein"
     gene            complement(6261..6440)
                     /locus_tag="HV345_RS26065"
                     /old_locus_tag="HV345_26085"
     CDS             complement(6261..6440)
                     /locus_tag="HV345_RS26065"
                     /old_locus_tag="HV345_26085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022310.1"
                     /translation="MKFRASAICDLMGVSRRQLDRWIKAGTFPLPDGRDAKGYWWEADR
                     VATRMISERKERGM"
                     /name="hypothetical protein"
     gene            6827..9586
                     /locus_tag="HV345_RS26070"
                     /old_locus_tag="HV345_26090"
     CDS             6827..9586
                     /locus_tag="HV345_RS26070"
                     /old_locus_tag="HV345_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="WP_053266538.1"
                     /translation="MAIKIPVSAQFDAADLQQQIKMVNDQIRILASQVGNANKQKWEPI
                     NLKSKDDLQGFIKQMDLLMKKQTDFATKMKQTGQGGNPLFADFKKMAEGRLGEQIKYME
                     SVLGWAGVEFSNLPAPKPPKKPPVVPPAPPSPPEPAWRKELGRQGMSGLHSILGNAGPI
                     GGTISNALNTGMSSGASAGMAGLVGGLAALGVGKIIGAIADKIGQAQDNAIGLDKLYRQ
                     VGGMVAYSNLRSGVGDIANRLGMNNADAIGMASTYTRASNLRPGMNLGTGLLVSGGLAR
                     GYGLDPNSVAASFGSLGATGVTNSDQSIRRTGMMMGEAIAKSGAFAQAGDAMTAITDFA
                     NTQARMSLSQPNIAGYGGALAGLMSSGVPGMDLQGAGSLLNRMNGAVMHGGNMGEASQA
                     FSARLGLRRGINDPMALQALQEGGAFSTVSSMLGKGSLYNQTTGKSFSGNETMLDMIRH
                     DIHRQYGYGSNATKALANHLGIGVNEAMLVDQMSSRDINGASSRLKRLGIDEKSINMSS
                     LPVLGQIESGKGLEALGNQYLKRSDLSKEERRGLIDALKGANPEKLKDALAEVASKHGG
                     TTTEGSQIRDNIAKLNNTLEDYSKRALPALDMMRMALVNMTGGSEASVRKQYYDSNMRE
                     ASSEIGAKYKDRRAAIQSQIDALTAKGVGLQSSGPDREKLVKLKAEMQSLNDQEGADRT
                     RAAERIKLQTYGASSSTEAALSTGDGYIQSPAAAAANGGGGKYRLNSDMKKRLERLRPY
                     FEEAGRKYNIDPRRLEAIAATESGFNNNLTSSKRAQGIMQVLPTSAPGANLSDERENIM
                     AGAMVYSQMRDRYGYLGQDEILRGYNGGSRRGSAENVAYPGKVNSYYRDLAQVDDMTPG
                     SNGNKGAVGEMYGTMRVVHEDKEGKQVKPPESVSMSGTFIQPSSWGNR"
                     /name="transglycosylase SLT domain-containing protein"
     gene            complement(9616..9813)
                     /locus_tag="HV345_RS26075"
                     /old_locus_tag="HV345_26095"
     CDS             complement(9616..9813)
                     /locus_tag="HV345_RS26075"
                     /old_locus_tag="HV345_26095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_154816742.1"
                     /translation="MSEFAVSRLVIPHVVIPPAVVEARRVRGVAAGHARKRRLAEVMAS
                     PTWYWSRRESPRLATTKKGA"
                     /name="hypothetical protein"
     gene            complement(9810..10364)
                     /locus_tag="HV345_RS26080"
                     /old_locus_tag="HV345_26100"
     CDS             complement(9810..10364)
                     /locus_tag="HV345_RS26080"
                     /old_locus_tag="HV345_26100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022319.1"
                     /translation="MGYFDCVKLTDAEWPDHMVEGRDAALRQAMRGGVSQWCRDKVVQL
                     LPVLRCPRGEWRDAELKRVDELADALGLDDMGLFAYGAMIVKRRAYVAGSLSRAKPPEP
                     LAVVLDDGEHRCTVYAMPHPTLGVPEISERGEDGYWTISAGGCAWIKARVSLGEGVRLW
                     NELVNCARYPDAPVLTRGMLE"
                     /name="hypothetical protein"
     gene            complement(10343..10639)
                     /locus_tag="HV345_RS26085"
                     /old_locus_tag="HV345_26105"
     CDS             complement(10343..10639)
                     /locus_tag="HV345_RS26085"
                     /old_locus_tag="HV345_26105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022318.1"
                     /translation="MSLYPKDKNPVFQPGGYGLRYDGQAEAIARAGEPPINYAPGQELS
                     RAAPALPEHPTHGAARAFRDHLTGEYVVAAGPVEVRTRVSAEEAALLWATLTA"
                     /name="hypothetical protein"
     gene            complement(10650..11018)
                     /locus_tag="HV345_RS26090"
                     /old_locus_tag="HV345_26110"
     CDS             complement(10650..11018)
                     /locus_tag="HV345_RS26090"
                     /old_locus_tag="HV345_26110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181636737.1"
                     /translation="MGRVKGLPRHLQGKTRLPLLGGCFPKGHRLALLAVMPIIEAHPGE
                     RHEGEAAKLALFQQLLLDGGQPPAFALHEPSLHRFGAALRSSRRGLTDAGVDKANELLD
                     QHLFSRALDAIVATKQTD"
                     /name="hypothetical protein"
     gene            complement(11006..11404)
                     /locus_tag="HV345_RS26095"
                     /old_locus_tag="HV345_26115"
     CDS             complement(11006..11404)
                     /locus_tag="HV345_RS26095"
                     /old_locus_tag="HV345_26115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249905.1"
                     /translation="MSMFDQLVARLRDEQALTKSLNPGDALAAVGNGDANAPVLDQRAL
                     TIGVAPGVVACHNARLNQIADAQLLQEVRASGVVSAADAVALDNALAASRHLPPEARAA
                     GMESVRQQLNAAAAAPLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            complement(11404..12105)
                     /locus_tag="HV345_RS26100"
                     /old_locus_tag="HV345_26120"
     CDS             complement(11404..12105)
                     /locus_tag="HV345_RS26100"
                     /old_locus_tag="HV345_26120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181636738.1"
                     /translation="MRERTMENEQIAPVDFEGANRIYLADVLAEACSALPELPDLPVPS
                     LDGDDAAVASLLHLMRQRDEMASAIGVLNFELGKLKEAAEAAHDRLVDAEARIAAARDA
                     LLAGNEPEPRDAMLALVVDQIRADVQAADAAVDIKSREVGALDAQMNTAVHQIEDAIDE
                     LICSCRIARARALELALLNELRALAVHDGDKNRFNRAFVPAPELALLSSNYGQIEMLRP
                     GRMPHLNRGVL"
                     /name="hypothetical protein"
     gene            12522..12674
                     /locus_tag="HV345_RS26105"
                     /old_locus_tag="HV345_26125"
     CDS             12522..12674
                     /locus_tag="HV345_RS26105"
                     /old_locus_tag="HV345_26125"
                     /inference="COORDINATES: protein motif:HMM:NF014814.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_181636739.1"
                     /translation="MARPTVITPELRETILQRHAIGVPAARLARQFGIGKTTVFRILAK
                     AKEQQ"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 aaaagacgct aaccctggag ggagcgttta cctttgagaa gatctccgcg cagtcggcag
       61 agttaatggc gcacctcttc ttttccagaa agaactcagg actgaaaggg tttgatggtg
      121 cgactgaatt ttttaacgtc gacgccaaaa aggcgtgcgc atttttcgag gaggccggag
      181 ggatacggca accgcagtaa aaaagagcgc ggtggccgta ctcgctaaag gtaccaccgc
      241 accccagcca gggaagccct aggaagcgac cccggcacgt aaccccaacc aagaggtaaa
      301 cgacatgcag agcatacagc agacaacccc tgcccgctac ccgggcctga gcattgaaaa
      361 cggagagatt cgccagcacg gaaagcagcg ccactgcgcc gccaaccgta aaggccagta
      421 ccagaccacc ctgcgcgaca gcgccggtcg gtaccagacg gtcagtatcc tcagccttta
      481 cgcatgggag gcatgatcat gatcccaggc agaacacaga tcctgaaagc caccgccgac
      541 atagccaagg ctctcggtac tgacaccgcc gatgtagagc gccacttcct gcagcacgcc
      601 gtcgtcctcg cacggctccg gcaggaggga aagcgccccc gcatcaaagc gtggatggca
      661 gagaacatcg gcaccccgtc agcgatgtaa gaaaaactta aatgttaagg taaaacaaca
      721 tacggtttta cttacgcatt aacgaaaacc atacacccgg ggcgcgagga gcgcccccac
      781 aggacacagc atgagaacca aggccatcat cgccgcagtc cttctcgcca ccgcaggaac
      841 cgccgcagcc gcaccagtcg ccacgaccta catctgcaac gacgggacat ttatccacct
      901 taacgagcag aatggcagac tcaccgccag ctggcgcggt gccgacatcc ccgtcaccaa
      961 gatcgagaca ggcaagctaa ccgccagcct caacggtgag cagggcagct caacactgca
     1021 gctcgtcgtc gggtacaaca ccacacccaa cccgatagcc atgattacca ccatcgtcaa
     1081 cggcagcatg aacagccaga gctgcaagcc agataccgca agtaagcaca tcgacctata
     1141 gcaacacgaa ggagagagca atggcatttg aaaacatcga gcgccggttc cagagatgga
     1201 aggtgatcat ggttcccgga gcgaagtcat acgagtcatg ggatgagatg gtcacaggcg
     1261 agaacttccc cgaggaagcc cggtacaaat acaagacgca ccgcaccaag gcgatcgacc
     1321 tcacgctgaa aaacccggac tacgtccaca ccatgtcgga gaacgggatc actgtcaggt
     1381 accggctcga acgctggcgc taaccccaac caggagcgcg gcgacagctg cgcccctacc
     1441 ggttagaaag cacgccccca ccagaacgca ccagagccat tctaagcgcc tcaaccccga
     1501 accaacccaa cgacacgaca gcaccccatt taagcccctg taccccctga aagttggggc
     1561 aggatttggc gaaccggtag gcgcggcgat tgccgccaaa caccaatgca cggtgccaac
     1621 cacgatccac cgtgcataaa ccacccgcca acccccgcca gcactggaaa ctgcccccat
     1681 ttccctccgg gcttttttag ctggagatcc ggcaggtgca aaccacgcac cggggagagc
     1741 gacaccagat ccaaacacga cacgcaaagt gcatccccaa cggggtgcag ctgcggcgcg
     1801 tcaactgcgc aggaattgag ctggaaccgc cttggaacct tgtggaaccg ccttggaacc
     1861 gccttggaac cttaaaaaat cttttaaaat caacgatgga accttggaac ctatttttta
     1921 aaaagagtaa tcagggaaat tgagagagct ttcgttattg gagagagaag gttagaaaat
     1981 caggttccaa aggttccaac cagcaatggc gcgggtttaa gaaggttcca gtgaggttcc
     2041 agcgaggttc cagcgaggtt ccaagccata aaaaaacccg ccgaagcggg ttattgttca
     2101 atttttgtac cggaaccggt ggacgttgtc gggcatcgag ccggacacga acgggatgag
     2161 cacccccttg ccttcagcag cgccaccaac atcgcggtat tttttcgcgc agccgatgat
     2221 cagatcgcgg tcatgcatca ggcgagcacg gcgcagcggt tcgcttttgt ccacgtacca
     2281 gtcgaggaac ttaccccagc atccgacagg agagcggaga accaaaccag cctttgattc
     2341 gaaggtgagg aagtccgggt gcccatcagc aacacgccca tggacgaacg ccacccaatc
     2401 agcccgaatt tcgtcatacg cgtcggtgat cagcccggca gcattcgcca acccatgcgc
     2461 agtggtgaaa gcagccagaa catggtcggc atcgcggcga gcggtctcaa agcccttagc
     2521 ctgataggac gccacccggg cattcagctc ggcggcaaag aacgccgtca ggctgtcgac
     2581 atagcgcccc ttatcagcga ggaacagcgg gcgcgtcttg atgtcgccct gcaggtcgag
     2641 catggaaaag cggttcgcca cctgcgggtc aattccgtcg gagtcggaca gtccggggat
     2701 cgggtcggca ctggtgaaaa ttttcatgta caggttcacg cactgcgtcg tgaggtactt
     2761 cggcgtcaga gccagcgact cctcaagacg aaagtgcttt ttcgtcaccc tggtgcattc
     2821 gttgacgatc agcgccagcg cccccatgaa gttacccgcc gttacaccgg agttggcacc
     2881 gctcaggatg ttgagcagct ccccctcgga cagctccacc gtcgccccca ggcgcgacag
     2941 aacgccgcca gcaccaaaca gcagaccctt accccaatcg gactgcgcac ggaaaaacag
     3001 gtaggcattc ttacggcttg aggcaaagcg ggcggcaacc agcgcgtcga ggaactcatg
     3061 gcattgaggg aagtgaacca gccaatcagc caccacgtcg ctgtcggcat tagccaccgg
     3121 atagggagca tgaggacgga cgaacgtcac cgtttcaccc accagttcga cgcgaggagc
     3181 cgtggcgaac atgtccacgt cgaggcgaac gaccgctacc tgacggcgca gcatgatgaa
     3241 ctcagccacc gcctgacgaa cagcccgggc catgtcgata cacgccttgg tcaccttgtc
     3301 gtcggtcttg attttgaggt cagtcaggcg ggcgctgaac gcatcggtgc ggtgccagaa
     3361 cttgccgcag gagtcctcca gcagcttcat cccgtcgccc ttgtcgaact cggtcaggga
     3421 gccattcagc acagagagga accgcccctt gccagcgtgc cagacggcag tgcagacagc
     3481 agagttgata gcttttacgt tcaggccata cagctcgaat tgagcgcggt agtcacccga
     3541 ccaatccaca gacaccgaca agacagaaaa cagcgcatcg ctcaacttaa gcgacagcgg
     3601 tgacggtgta gcggcgatcg gcgttacagt ggcgagagca gcgccggaat tatcatcagt
     3661 ttgtgacacg caattactcc tttaaaaacg caggagtcgg catcagacgg gactttagcg
     3721 gaggaaatcg acttgcacga tcttatcgac gtgatacaat tctcagcgag gatttgagaa
     3781 tcacccaacg tgatgtatga tttccttcga gttcgtccgt caagacacta actcgaaact
     3841 acggggttta accccggcaa tcaaagcacc cggccaggtg ctttttttgt atccgcagat
     3901 cacccaacac gatccacgat ccacgaacga cgatcagctt aaccgatcct aacccttcat
     3961 gccactgaac ggcgaccgct tatcgagctg tcgtgatcgc gccgcgcttt tatccactgc
     4021 aacgtcaaac tgtccacagg gcttgtccac aggcaccaga gaggcgagcc gaagggctcg
     4081 acccgtaccg attagagaag cacgtcagga gcacgtcgaa atagacgcgg aagcgcaaac
     4141 atgctccaat gagaggcata atcagagcac aggagctata cgcatgaagc gcaaagcctc
     4201 gacctttggc ctacgcctcc ccccggagct gctcgagcag gtgaacgagc tggcagagaa
     4261 aacgaaccga acccgcaccg acgtgatcac cgacgcactc cgcgcctaca tgggcataca
     4321 ggagccgcag ggggagagcg gcaaccgtct cgacctgatg gtggagctgc tgcaggacat
     4381 ctcgaatacg ttgaagcacg gcgtgccaca gaaagccgcc agacgcgcca cagcgccgcg
     4441 agccgaaaag cagtacggaa gcacgctcga tacacagaac gaagcacagc gcccggctgg
     4501 cgcggaaatt tcggggcatt acgacgaggc cgaggtgatg gccaccatac ggcgcatgag
     4561 ggaggagcag cgcgatcggg gattccgcta cgacaacaaa gctatcgccc aggcgctgaa
     4621 cgaggcaggg ctcctgcagt cgaacggtcg cccatggaac aacgaccgca tcaacacggt
     4681 gatcacccgc cggatgcctg acctgaagta accggtagcg cggcagccgc cgcccagggc
     4741 aaaggactga tcgcagccgg ccccggcgtt ttagtgcgag gcaccctgcg ccgcttgcag
     4801 caagccagcc tcaacaccca gcccaaggcc gatcaggcga tcgagctgtt gggcgaggtc
     4861 gaacgcggca gtagcctgca gctgcgtggc gtagatcacc cccgccgtct gcaggaattg
     4921 agccagcaca gccgggtgag cagcggcata gccctcgccg aacatggcgt ccagctcgac
     4981 gatagcctga gagacgagga aagcggattg tgagagcgcc aggtcggcga cggtggtggt
     5041 ctggtcggtc atggggagat cctcaaataa aagcccgctg aagcgggctg ggcacaacga
     5101 gagagaaaac tggtaccact acaggctcgc gtcacgcgcc tcccgaaaca agccccgcag
     5161 gtacttgcga cccatctcga cgccgtgacg cgccagcaga tcgaacgggt cgtagccgtc
     5221 cacgccctcg gctcccggta ccgccgacag gtcgatcacc cgggcattcg ggatagcctg
     5281 atcgatagcc agatcctgcc cgccaatgtc cggtaccagt acgaacgggt ggccccccat
     5341 aacaaactta ttcagcgact gcaccgttgc cccgaagtta cccaccgaca tcgcgcagac
     5401 caccgggagc ttgccgccaa tcagcaggga gagcgccagc cccgacatga acccctcgca
     5461 gaccaccaca ggagccccgg aaccgacagc atccagatcg aacgggtaga acagcccctc
     5521 acgcggcgca ttcttgagcc cgcgcttctc gcccagcgaa cccgccgcct ccgggttagc
     5581 ccgaccgcaa atcacctcga ccgacatctg ctccagccgc agggactcat tcaccccgta
     5641 cagcggcacc aggagcgagc tgttccagta cacctgaccg attacaggca ggaacgtgcg
     5701 ccagtccgag cacttcacgt catccgggca cggcaaaggg agacacgccg cccgctcttt
     5761 ggcttgccag tacagcgcag cactccctgg tttggttgcg cgatgcagca gcatccgcgc
     5821 cctaccggtt gccttaatgc gagcctccgt cagcttctcc gcagcagccg ccgcacgttc
     5881 ccgtaacgca accagatgcg cctggcgagc cgccagctcg tcctcggacg gctcacgcac
     5941 agcaatacgc cccgcggcaa cgtcagcagc gaccccggag gagcgcagga acgccccagc
     6001 agccgccagc cggtcgagtt catccaggtc acgcagcgcc gacaccaaca tagccctgtc
     6061 gccctttccc gcattcacac cacgcgcaga ccactccaca caggtctcca gcggatccat
     6121 ccccagcagt tcacaccagc acgccagcgt gcgagcctga gccagccagc ccataaccga
     6181 cggatccggg tctacacgcg ccagcgccgc cgagaacacc gcgtggtcaa aaaggtaatc
     6241 tcgtctctcg ttcaccataa tcacatcccc cgttctttgc gttcggagat catccgcgtg
     6301 gccacccggt cagcctccca ccagtacccc tttgcgtcgc gcccgtccgg cagaggaaag
     6361 gtgcccgcct tgatccagcg atcgagctga cgacgggaca cacccatcag gtcgcagatc
     6421 gcgcttgcac gaaacttcat ggaaccccct gtagaaaagc cccgccgtga gtggtcgggg
     6481 ctttgagtga tgactattgc gatgcagtcc ttatgggctt atcgccggag cgacagggct
     6541 gcgccattcg cgcttactcg cttttcacca ggtggaagtg cgccaccgga agtagaccga
     6601 agtccaggca cccgagcgat ccgggccatg tttatccaac tgctattgga tggtgtctgt
     6661 tttagactcg ttttagacat tttacaagtt ctatttttca aagaatccag acgtggagcg
     6721 gcttgcgcgt cgatttcgac gcattgcgtt ttagacacgt tttagacatt taaccccaaa
     6781 cgtcgtgacg ccagcatgag gaggcttaca acagagagag ataactatgg ctatcaaaat
     6841 tccggtctcg gcgcagttcg acgctgccga cctccaacag cagatcaaaa tggtcaatga
     6901 ccagatccgc attctggcca gccaggtagg taacgccaac aagcagaagt gggaacccat
     6961 caacctcaag agcaaggacg acctgcaggg cttcatcaag cagatggact tgctcatgaa
     7021 gaaacagact gactttgcca ccaaaatgaa acagaccggg cagggcggta accccctgtt
     7081 tgccgacttc aagaaaatgg ccgaaggtcg cctcggagag caaatcaagt acatggagag
     7141 cgtgctgggg tgggcgggcg tagagttcag caacctgcca gcaccaaagc cccccaaaaa
     7201 gccgcctgtc gtgcccccag cgcctccgtc gccgccagaa cccgcgtggc gcaaagagct
     7261 ggggagacag ggaatgagtg gcctccacag catcctcggc aatgccggcc ctattggcgg
     7321 tactatcagc aacgccctga acaccggtat gtcttccggc gcgagtgcgg gcatggcggg
     7381 tcttgtcggc ggtctggcgg cgctgggcgt cggcaaaatt atcggcgcta tcgctgataa
     7441 gatcggacag gcgcaggaca acgccattgg gctggataaa ctgtaccgcc aggtcggcgg
     7501 catggtggcg tacagcaacc tgcggtcagg cgtcggcgac atcgccaacc gtctcggcat
     7561 gaacaacgca gacgcgatcg gcatggcgtc tacctacacc cgcgcatcca atctgcgccc
     7621 cggcatgaac ctagggacag ggctactggt aagcggcggg ctggcacgcg gttacggact
     7681 cgatccgaac agtgtcgccg cctcctttgg ttccctcgga gcgacaggcg tcactaacag
     7741 cgaccagagc atccgtcgaa ccggcatgat gatgggggaa gcaatcgcca agtctggcgc
     7801 gtttgcacag gctggcgatg caatgacggc gatcaccgac tttgccaaca cccaggcacg
     7861 catgagcctg tcacagccaa atatcgccgg gtacggcggc gcactggccg gacttatgag
     7921 ttccggcgtc ccgggcatgg atctgcaggg agccggtagc ctcttaaacc gcatgaacgg
     7981 agccgtcatg cacggcggca acatggggga agcgtcacag gcattcagcg cccgtctcgg
     8041 actgcgccgg ggcatcaatg acccgatggc gctccaggca ctgcaggagg ggggagcgtt
     8101 ctccaccgtc agcagcatgc tgggcaaggg gagcctctac aaccagacca ccggcaagag
     8161 cttcagcggc aacgaaacca tgctcgacat gatccgtcat gacatccacc gccagtacgg
     8221 atacggctcc aacgccacca aggcactggc gaaccacctc ggcatcggcg tcaacgaggc
     8281 catgctcgtc gaccagatga gcagcaggga catcaacggc gcgtcgtccc gccttaagcg
     8341 cctcggtatc gacgagaaaa gcatcaacat gagctccctc cccgtcctcg ggcagatcga
     8401 gagcggcaaa ggcctggagg cactcggcaa ccagtacctc aagcgcagcg acctgtccaa
     8461 agaggagcgg cgcgggctga tcgacgccct taaaggcgca aacccggaga agctcaaaga
     8521 cgcgctcgca gaggtcgcct cgaagcacgg cggtaccacc accgagggga gccagatccg
     8581 cgacaacatc gccaaactca acaacaccct cgaggactac tcgaaacgcg ccctgccagc
     8641 cctcgacatg atgcgcatgg ctcttgtaaa tatgacaggc ggctcagaag ccagcgttcg
     8701 taagcagtat tacgacagca acatgagaga agccagcagt gagatcggcg caaagtacaa
     8761 agaccgccgt gccgccatcc aaagccagat cgatgccctg acagcaaaag gcgtcggcct
     8821 ccagtcctcc ggtccggaca gggagaagct ggtcaagctc aaggccgaga tgcagagcct
     8881 gaacgaccag gaaggggctg accgcaccag agccgcagaa cgcatcaaac tacagacata
     8941 cggggcgtcg tcctcgacag aggcggcgct gtctaccggt gacggataca tccagtcacc
     9001 agcggcagca gcagccaacg gtggcggcgg caaataccgc ctcaacagcg acatgaagaa
     9061 acgcctggag cgtctgcgcc cttacttcga ggaggccgga cgcaagtaca atatcgatcc
     9121 acgtcgcctt gaggccatcg ccgccactga gtccgggttt aacaacaacc tgaccagcag
     9181 caaacgtgcg cagggcatca tgcaggtact gccaaccagc gccccgggag ccaacctcag
     9241 cgacgaacgc gagaacatca tggccggtgc catggtctac agccagatgc gtgaccgata
     9301 cggatacctg ggacaggacg agatcctgcg cggatacaac ggaggtagcc gccgtggcag
     9361 cgcagagaac gtcgcctacc ccggcaaggt gaacagctac taccgcgatc tggcacaggt
     9421 tgacgatatg accccgggca gcaacggcaa caaaggcgct gtcggcgaga tgtacggcac
     9481 aatgcgcgtc gttcatgagg acaaggaggg taagcaggtc aagccgccag agagcgtcag
     9541 catgtccggc acgttcatcc agccaagcag ctggggcaac cggtagcggc gcagcagcgc
     9601 ccaaaagaga ggggcttacg cccctttttt tgtggtggcc agacggggag attcacgccg
     9661 cgaccagtac caggtaggtg atgccatgac ctcggcaagt cggcgctttc tggcgtgccc
     9721 tgcagcaaca ccgcgaacgc gtcgagcctc gacaacggcg ggagggatga ccacatgggg
     9781 gatcaccaga cgggatacag caaactcgct cattccagca tcccccgcgt caggaccggc
     9841 gcatcagggt accgggcgca attaaccagc tcgttccaaa gcctgactcc ttccccgagc
     9901 gagacgcgag ccttgatcca tgcgcaaccg ccagcggaga tagtccaata cccatcctcg
     9961 ccgcgttcgg agatctccgg cacgccgagc gtcgggtggg gcattgcata gacagtacaa
    10021 cgatgctcgc catcatcgag gacaacagcc agcggctcgg gcggtttagc ccgagagaga
    10081 ctaccagcaa cgtaagcccg ccgcttcacg atcatagcgc cgtaggcaaa cagccccata
    10141 tcgtccagcc ccagggcatc agcaagctcg tctacacgtt tcagctcggc atcacgccac
    10201 tcgccccggg ggcaccgcag caccgggaga agctggacaa ccttgtcccg gcaccattgg
    10261 gagacgccac cacgcatggc ctggcgaagg gcggcatcac gcccctcaac catgtgatca
    10321 ggccactccg cgtcggtcag ctttacgcag tcaaagtagc ccacagcagc gcggcctcct
    10381 cagccgatac gcgggtacga acctcgaccg ggcccgcggc gacgacgtac tcgcccgtca
    10441 ggtgatcacg gaacgcacgc gcagcgccat gcgtcgggtg ctcaggcagc gcaggagcag
    10501 cacgggacag ctcctgaccc ggggcgtagt tgatcggtgg ctcaccggca cgcgcaatgg
    10561 cctcggcctg accgtcatag cgcagaccgt agccgccagg ctgaaacacg ggatttttgt
    10621 ctttcgggta taagctcatt ttttaacctc taatctgttt gtttagtcgc cacaatggca
    10681 tcaagggcac gggagaaaag gtgctggtcg agcagctcat tggccttgtc gacccccgca
    10741 tcagtcaatc cgcgtcggct gctcctcaac gcagcgccga agcgatgcag ggacggctca
    10801 tgcagcgcga acgcgggcgg ttgccccccg tccagcaaca gctgctgaaa caacgccagc
    10861 ttcgcagcct ccccctcgtg tcgttcacca ggatgcgcct cgatgatcgg catgacagcc
    10921 agcagcgcca gccggtgccc cttcgggaag caaccaccca gcaacgggag ccgcgtcttg
    10981 ccctgcaggt gacgcggcaa gccttttact cgtcccaatt gataccccgg gcagcaggac
    11041 tgagaggagc agcagcggca gcgttcagct gctgccttac cgattccatg cccgcagcac
    11101 gagcctcagg aggcaggtga cgcgaagcag ccagcgcatt gtcgagcgcc accgcatcgg
    11161 cggcagacac cacaccagag gcgcgaacct cctgcagcag ctgcgcgtcg gcgatctggt
    11221 tcaggcgggc gttatggcac gccaccacac caggagcgac accaatggtc agcgcacgct
    11281 gatccagcac gggtgcattc gcgtcgccat tgccgaccgc agccagcgcg tcgcccgggt
    11341 taagggactt ggtcagagcc tgttcgtcgc gcagacgagc aaccagttga tcgaacatgg
    11401 acatcacagc acccctctat tcaggtgagg catacgcccc ggacggagca tctcgatctg
    11461 gccgtagtta gacgacagaa gcgccagctc aggagccgga acgaatgcac ggttaaagcg
    11521 gtttttgtcg ccgtcatgta cagccagagc acgcagctcg ttaagcagag ccagctccag
    11581 agcacgagcc cgggcgatac ggcaggagca gatcagctcg tcaatggcat cctcgatctg
    11641 atgcacggcg gtgttcattt gcgcatcgag tgcccccacc tcccggctct taatatccac
    11701 cgcagcatcg gcggcttgca cgtcagcgcg gatctggtcg acaaccagcg ccagcatcgc
    11761 atcacggggc tcgggctcgt tgccagccag caaagcatca cgggcggcgg cgatacgcgc
    11821 ctcagcatca accaagcggt catgcgcagc ttcggcggct tccttcagct tacccagctc
    11881 gaagttgagc acaccgatcg cgctggccat ctcgtcacgc tgcctcatca ggtgaagcag
    11941 ggacgcaaca gcagcatcat cgccatccag cgacggcaca gggagatcag gcagttcagg
    12001 gagggcagag catgcctcag cgagcacgtc tgcaaggtag atccggtttg cgccctcgaa
    12061 atcgacgggg gcgatttgtt cgttttccat agttctctct ctcaatttag ccggaacggc
    12121 tacgcctatc gttgcgtcac gacaggtctg gagccagcgc gggagagtag aaccggtagg
    12181 cggcgcggcc tttcgccacg ccctggggca ggacgcgaga ttgtgcgaaa acgggggata
    12241 tttaacataa tggacgttac gcgaaccacc ggatcggcac tcgaccagca aacggcacga
    12301 acccgctatt ttcggcactt aaaaagggag tttgagcaaa aaagcagcgt cacatcatgc
    12361 ttaaaaagcg cgtttttatg cactttgaaa tttacgccgc gaaagcgcca ttttcgacac
    12421 ttttcacccc tgcgaggcgc tgctaaaaag cattgactat gcccgccggg tagtcgtaaa
    12481 tgtaatcagg tcgaaaccga cccaaaacca caggaggcca tatggcacgc cccacagtga
    12541 tcacccccga gctgcgagaa accatcctgc agcgccacgc gatcggggta cccgccgcaa
    12601 gactggctcg ccagttcggc atcgggaaaa cgacggtgtt tagaatttta gcgaaagcga
    12661 aggagcaaca gtgagaattt actcatacct aacaagagat ggcagcgaaa aggtcaacat
    12721 gcagatcttc agggcactgg aggccgtaag tgaaacccta agcattccat tagacgtaac
    12781 gatccccgtg gaggagcgcg gagaggtgct ggaaagactg gtaagcggag cagcaaccgg
    12841 agacatcatc atcatcacgg aggaacagcc cctctacgac atgacaaaac agcgggcaga
    12901 actcatccgc agcatcatcg aggagaaagg gatctacctc ctaaggccat tcagtgacgg
    12961 gtacgaacca ggatacacgc aagatctcag cgacgtggcc agccttgacg agtcaggtag
    13021 acacggagca ctcatggtgt cgaccagcat caacctgtac ggcatgtgga catacgtgaa
    13081 gcacgagaag atagcggtca caccggactc aggagtacca ctactgccgc cagccccaag
    13141 ccttgcatac gtgatcagag cggggaagaa cctctgcaag atagggtaca gccataaccc
    13201 gaaagggcgg gtaagcagca tcaccagaag catgactgaa gcgggtgcca ttggca
//
