LOCUS       NZ_CP056198             1917 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00995 plasmid pRHBSTW-00995_8,
            complete sequence.
ACCESSION   NZ_CP056198
VERSION     NZ_CP056198.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148764
            Assembly: GCF_013782105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00995
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 139.174696581201x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:27:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,523
            CDSs (total)                      :: 5,399
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 83 of 154
            Pseudo Genes (incomplete)         :: 83 of 154
            Pseudo Genes (internal stop)      :: 23 of 154
            Pseudo Genes (multiple problems)  :: 28 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056198.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00995"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00995_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            459..761
                     /locus_tag="HV347_RS27445"
                     /old_locus_tag="HV347_27455"
     CDS             459..761
                     /locus_tag="HV347_RS27445"
                     /old_locus_tag="HV347_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     gene            complement(1021..1257)
                     /locus_tag="HV347_RS27450"
                     /old_locus_tag="HV347_27460"
     CDS             complement(1021..1257)
                     /locus_tag="HV347_RS27450"
                     /old_locus_tag="HV347_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(1423..1596)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2..355
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.414"
                     /coverage="99.0"
                     /evalue="1.15e-74"
     Typing          1569..1917
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.761"
                     /coverage="95.0"
                     /evalue="1.05e-134"
ORIGIN
        1 ctcagttccg cgaaggcagt tcgctcctgg atggactgta tgcacgaacc ccccgttcag
       61 tccgactgct gcgccttatc cggtaactat catcttgagt ccaacccgga aagacacgac
      121 ttatcgccac tggcagcagc cactggtaac tgatttagag gagttagtct tgaagttatg
      181 cgcctgttaa ggctaaactg aaagaacaga tttggttact gcgctcctcg taagccagtt
      241 accacggttc aaagagttgc tgctctgaga accttcgaaa aaccgccttg cagggcggtt
      301 ttttcgttta cagagcaaga gattacgacg ccacagaaag gatctcaaga agatccttca
      361 ccgtaatgga aatctatcca atccgagcac ataggataca catcgttata aaacatcgga
      421 tattatgatc ccaattaaaa taagctcgag gataaaaaat gagttcaatg gtttactgcc
      481 gaggatgtgg caaggaaata cacgaaacag caaagtcatg cccgcactgc ggagccacaa
      541 acgcctcttc aggttcaggt gaaaaaagta gaatcgcagc cgcgctatta gcattcttcc
      601 ttggtggatt tggcgcacat aaattttact taggaaaaat aggacagggg ttcctgtatc
      661 taattttttg ctggacattc atacccgcga tcatcgcgtt tattgagttt attatttatc
      721 tatgcgattc cgatgaaaaa ttcgcacgaa aatatggcta actcatagca aaaaaaagcc
      781 ccctaagggc ttttttttgt acctgacggt cagaccgatt aaacataaca tcccttgccc
      841 gcacccccgc aggcgccctc agcgcaatcc tgctgcatca gggccgtttc cagcaccttt
      901 cctcaccagt atcccggaaa aacgcccctg atgatttctg cgcgactgca tggctatgca
      961 cgcaggtgaa tggcaggtga tttcgcagcg tcagcaccac taaaccgcca aaagccaaaa
     1021 tcagcggcgc ggcgcgcgcg gacgtgacca ggcaaaatcg gactgctgcc gctcatcctc
     1081 acgtaatgca cgctggcggt cggcgtaacg ctgcaactca atttgcgcac gcgctctgtt
     1141 caggacgatg tgccggttag gctgatttag ctttttacct gacgctcttt cagctgcggc
     1201 aacagcatca tcaagaaatt ttttctctgc aggcgtcaag tttgtcagtc tggccataac
     1261 gttctccagt cagaataaat cagcacagtc tgccagacac agtagaaacg atgaagcgcc
     1321 agcgacggtg aaaggcagtc agaaacggtg aacgggagcg gattttaatc cggtgccgtg
     1381 aggcgttgga caagccgcag gcgcgtcatt agcttttagc gggtttcggg gcgcagccct
     1441 gaaccagtca cgtagcgcta gcggagtgta tactggctga ctatgttggc acggatgtgg
     1501 aaatcagtga agtgcttcat gtagcaggag aaaaaaggcg gcaccagcgc gtcagcagga
     1561 tatgtagtac aggatatatt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1621 gctgcggcga gcggtgccgg cttacaggcg gggcggaaat ttcctgggag ataccaggaa
     1681 ggagcttaac agggaagaga taaggccgcg gcgaagccgt ttttccatag gctccgcccc
     1741 cctgacaaac atcatgaaat ctgacgctca aatcagtggt ggcgaaaccc gatgcggctt
     1801 aaagatacca ggcgtttccc cctggtggct ccctcgtgct cccccgcatt cctgcctttc
     1861 ggtttaccgg tgtcattccg ctgttatggc cgcgtttatc tcattccacg cctgaca
//
