LOCUS       NZ_CP056197             2426 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00995 plasmid pRHBSTW-00995_7,
            complete sequence.
ACCESSION   NZ_CP056197
VERSION     NZ_CP056197.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148764
            Assembly: GCF_013782105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00995
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 139.174696581201x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:27:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,523
            CDSs (total)                      :: 5,399
            Genes (coding)                    :: 5,245
            CDSs (with protein)               :: 5,245
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 83 of 154
            Pseudo Genes (incomplete)         :: 83 of 154
            Pseudo Genes (internal stop)      :: 23 of 154
            Pseudo Genes (multiple problems)  :: 28 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056197.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00995"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00995_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(1256..2426,1..89)
                     /locus_tag="HV347_RS27435"
                     /old_locus_tag="HV347_27445"
     CDS             join(1256..2426,1..89)
                     /locus_tag="HV347_RS27435"
                     /old_locus_tag="HV347_27445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_181589896.1"
                     /translation="MSYQFIHIESYARVGSNQKGKSTKWNIHQIAGEAMRNDEDCKHVE
                     HPQEPTVLFGVDAYAAADIAQKWAETSSDASGRKLRKDGSCLLAGVISFPEDRTEADWK
                     AFRDDSIKYLKEKYGENLKSVIEHRDEAFKHIHFYSVPKPGQKFEDIHDGKKAVLEAKK
                     GNSKILKGDQNRVYIQAMKEFQEDFYQNVAIKHGMTKTGPKRERLTREEWKARKEYALK
                     QSEEIKNISLLKDQAIHIGKKEGFDYSVEQSKGWGWIAKIGAKYKYYTDDFKKKLEEKD
                     EEIEVIKNQRNEQQKKHRAFKKEYYSQKKETEKAVEEKKEKSKEVYFLNNENKELKKNN
                     DILEVENLSLREENKILQQYKITSKKMRDRFPDQYKAMEAEINKPRPKGESYNPEPKAL
                     ELSIQQEEDITNQYRKRPRM"
                     /name="plasmid recombination protein"
     gene            534..1040
                     /locus_tag="HV347_RS27440"
                     /old_locus_tag="HV347_27450"
     CDS             534..1040
                     /locus_tag="HV347_RS27440"
                     /old_locus_tag="HV347_27450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_069067453.1"
                     /translation="MITKSRVHENVEDITEVLNSKKIKDKIDEMQERGDVKATVIIQDD
                     KKISLKNEFVILFLENFDRLITELKINTSELRVLIYILKKMEFGNLVSLSQASIVKALD
                     MNKSNVSIIFKKLLAKDVLIKDEDGNMYVNSNIVMKGLKHKLSGKNKENLQKSQKQTDL
                     FDKSH"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 gctataaccc tgaaccgaaa gctcttgagc tttctattca gcaggaagaa gatataacaa
       61 accaatacag aaaaagacct cgaatgtaga acattcatct ctggccgctt gtagtgatgt
      121 ttgagacatc acaataggcg gcaatgatgg catagccagt agagatcagt tttcataacc
      181 gtgagaacgt cagaacctcc ggttctgggg ttcgaggggt tggcctgccc ggcgagggcg
      241 gttttcctct ttcttatgtt cttatatatc ttacttctta cacaccactc taagcctact
      301 cccacaaggc tttcactact aaaaagttgg taaatccaca aactttagtt tgtcacttta
      361 ccaaccatta gttggtggat ttaccaacca tagtttgtgc atttaccaac tctagttttg
      421 tgaacttccg ttcaaaaaag tagaaaaagt ttgttatttt taaacttttt ctattgactt
      481 atatttttga tcggttaaaa catatatatc agaatataaa aaaggagatt attatgataa
      541 ctaaaagtag agttcacgaa aatgttgagg atataactga agttttaaat agtaaaaaaa
      601 taaaagataa aatagatgaa atgcaagagc gtggagatgt taaagccaca gtaataatac
      661 aagatgataa gaaaataagt ttaaaaaatg agtttgtaat attattttta gaaaactttg
      721 atcgtttaat aacagagtta aaaatcaata cttctgagct tcgtgtattg atttatattt
      781 taaagaaaat ggagtttggg aacttggttt cattatctca agcatccata gttaaagcat
      841 tagatatgaa taaatcaaat gtctctatta tatttaaaaa gcttttagct aaagatgttt
      901 taataaaaga tgaagacggt aatatgtacg taaactctaa tattgtaatg aaaggattaa
      961 aacataaact atcaggaaaa aataaagaga acttacagaa gtctcaaaaa caaacagatt
     1021 tatttgataa atcacattaa taaaaaacta aatactctag cgcgctacgc ttgctgaatc
     1081 cccaaacatc ataaatcata gatttataat gcttggggat ttctgttttt tgtctttagc
     1141 ctccggcaag accgacgctt atcgttagat aagcatagtc ggtctctatg ctttactagc
     1201 aagcttattt ctgttagtta caaactatag gggaatatat atttaggagg aaactatgag
     1261 ctatcagttt attcatattg agtcatatgc aagagttgga tcaaatcaaa aagggaaaag
     1321 tacaaaatgg aatattcatc agatcgcagg tgaagctatg cgaaatgatg aagactgtaa
     1381 acacgtagag catccacaag aacctacagt tctttttggt gtggatgctt atgcagctgc
     1441 agatattgct caaaaatggg ctgaaacgtc ttcagacgct tcagggagaa aacttcgcaa
     1501 agatggctcg tgtttactcg caggtgtcat atcttttcca gaagaccgta cagaagctga
     1561 ctggaaagcg tttagagatg actctattaa atatttaaaa gaaaaatatg gtgagaattt
     1621 aaaatctgtt atagagcaca gagatgaagc atttaaacat attcattttt actctgtccc
     1681 aaaacccgga caaaagtttg aagatatcca cgatggaaaa aaagctgtat tagaagccaa
     1741 aaaaggaaac tcaaaaatac ttaaaggcga tcaaaataga gtctatattc aagctatgaa
     1801 agagtttcaa gaagactttt atcaaaatgt agctataaaa catggaatga caaaaacagg
     1861 gccaaagcgt gaacgcttga cccgtgaaga gtggaaggca agaaaagaat atgctttaaa
     1921 acaatcagaa gagataaaaa atattagctt attaaaagat caagctattc atataggtaa
     1981 aaaagaaggt tttgactatt cagtagaaca gtcaaaaggc tggggatgga tcgcaaaaat
     2041 aggagctaaa tataaatatt acactgatga ttttaaaaaa aagttagagg aaaaggatga
     2101 agaaatagaa gttatcaaaa accagagaaa tgaacaacaa aaaaaacata gagcatttaa
     2161 aaaagaatat tactctcaaa aaaaagaaac agaaaaagca gttgaagaaa aaaaagaaaa
     2221 atcaaaagaa gtttactttc taaataatga aaataaagag cttaaaaaaa ataatgatat
     2281 tttagaagtg gaaaacttat cattgagaga agagaataaa atattacagc agtataaaat
     2341 aacaagtaaa aaaatgagag atcgttttcc tgatcagtat aaagctatgg aagctgaaat
     2401 aaataaacca agaccaaaag gtgaaa
//
