LOCUS       NZ_CP056173             4783 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00695 plasmid
            pRHBSTW-00695_6, complete sequence.
ACCESSION   NZ_CP056173
VERSION     NZ_CP056173.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148685
            Assembly: GCF_013733395.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.5654070765511x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013733395.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:14:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,933
            CDSs (total)                      :: 4,818
            Genes (coding)                    :: 4,681
            CDSs (with protein)               :: 4,681
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 45 of 137
            Pseudo Genes (incomplete)         :: 74 of 137
            Pseudo Genes (internal stop)      :: 29 of 137
            Pseudo Genes (multiple problems)  :: 12 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056173.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00695"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00695_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4599..4783,1..139))
                     /locus_tag="HV268_RS24550"
                     /old_locus_tag="HV268_24555"
     CDS             complement(join(4599..4783,1..139))
                     /locus_tag="HV268_RS24550"
                     /old_locus_tag="HV268_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170864053.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            790..>912
                     /locus_tag="HV268_RS24930"
                     /pseudo=""
     CDS             790..>912
                     /locus_tag="HV268_RS24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1232..1588)
                     /locus_tag="HV268_RS24555"
                     /old_locus_tag="HV268_24560"
     CDS             complement(1232..1588)
                     /locus_tag="HV268_RS24555"
                     /old_locus_tag="HV268_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(1791..2108)
                     /locus_tag="HV268_RS24560"
                     /old_locus_tag="HV268_24565"
     CDS             complement(1791..2108)
                     /locus_tag="HV268_RS24560"
                     /old_locus_tag="HV268_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2295..2537
                     /locus_tag="HV268_RS24565"
                     /old_locus_tag="HV268_24570"
     CDS             2295..2537
                     /locus_tag="HV268_RS24565"
                     /old_locus_tag="HV268_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(3208..3423)
                     /locus_tag="HV268_RS24570"
                     /old_locus_tag="HV268_24575"
     CDS             complement(3208..3423)
                     /locus_tag="HV268_RS24570"
                     /old_locus_tag="HV268_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_137500696.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSYLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3430..3921)
                     /locus_tag="HV268_RS24575"
                     /old_locus_tag="HV268_24580"
     CDS             complement(3430..3921)
                     /locus_tag="HV268_RS24575"
                     /old_locus_tag="HV268_24580"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_048237138.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSLLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3956..4660)
                     /locus_tag="HV268_RS24935"
                     /old_locus_tag="HV268_24585"
                     /pseudo=""
     CDS             complement(<3956..4660)
                     /locus_tag="HV268_RS24935"
                     /old_locus_tag="HV268_24585"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(255..428)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          352..899
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.559"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(3..139)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.304"
                     /coverage="98.0"
                     /evalue="1.54e-21"
     Typing          complement(3117..4609)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4606..4781)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.22"
                     /coverage="98.0"
                     /evalue="2.32e-27"
ORIGIN
        1 gcgagattga ggggagcttt ccggtgcgcg caggcttttc gtccaggcag gtctgccgca
       61 tccaggccgc cagcggcctg ccgtcacagc gctcaagcag ccgccggtgc tcgtcctccg
      121 tcacccacat ggtgagcatc ttgttccgtt tctctgacaa accggcctcc tgataacgcc
      181 gggacgggcg ggaaagcctt tcccggtcgg cgcggggttg gacaagccgc aggcgcgtca
      241 gtggcttttg gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg
      301 tatactggct taatatgttg gcacggatac ggaaatcagt gaagtgcttc atgtggcagg
      361 agaaaaaagg cggcaacggc gcgtcagcag aatatgcgat acaggatata ttccgcttcc
      421 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtgcg ggcttacgaa
      481 cggggcggaa atttcctgga agatgccagg aagaaactta acagggaagc gataaggcca
      541 cggcagagcc ggttttccat aggctccgcc cccctgacga gcatcacaga atctgacgct
      601 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctgatg
      661 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
      721 ggccgcgttt gtctcattcc acgcctgaca ctcggttccg ggtaggcagt tcgctccaag
      781 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgcctgttc cggtaactat
      841 cgacttgagt ccaacccgga aagacacgac aaaacgccac tggcagcagc cactggtaac
      901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      961 tacggctaca ctggaaggac agtatttggt atctgcgctc tgctgaagcc agttaccagg
     1021 ttaagcagtt ccccaactga cttaaccttc gaaaaaccgc ctccccaggc ggttttttcg
     1081 tttacagagc aagagattac gcgtacagag cacggatctc aagaagatcg taagaagaaa
     1141 gttaacatga gcgtttttat ccctcaaaaa gaaggcttct agttatagaa gccttctttt
     1201 tgagggataa cttatatttt tttaaaaaaa atcatgcaat agcatgtttc ttctcagaac
     1261 tatcaacaat gtgtttcctt tctatgattt catgagcggg aatcactctg tctatttctt
     1321 cttgcaatcg agggctacta gtaacctccc ataaatcaac agactgctgt atatttagcc
     1381 agaactttgc agtggtatcg aaggctttag ccaacctaaa agccatatcg gtagacagct
     1441 ttctattatt gttaactaaa gcactggcag agtttctatg cacattcaac atttcagcca
     1501 agtcattgat ttttaaacca cttggttcga gatattcata aagaataata tcccctacag
     1561 ttgatggttt tctagttgct tgggacatgc tttactccta aattttttac ttctctctgt
     1621 tagatgagga agataagtat ttcgtctatt accagctgta gcgatgagta attatcttaa
     1681 atatagtcac gtcatttttt ttattaatca ttatttgtaa tgacatttta atgtaggtga
     1741 gggaacgcct cacctacttg ttttataaga taacagccac gacctgttat ctatcggtaa
     1801 tcatgtggat cgagatacaa atcctcgact ccgttttcac cccacttaaa tatcaagcgg
     1861 tacttctcat tcacccgtat ggatgaatac cctgtcaatg gagggttgag attctcaaat
     1921 cggtttcccg ggggagaccg caaatctcca actttatcag cagcattcag catatccagc
     1981 ttgcgaccta atgaagcact aatcgcagtt ggaatctttc gattggcttt accatatttg
     2041 taaaactggt atagccaatc gtctcgaaac tgaatgcggc aatcatcatg ttcatgatct
     2101 tgctgcattt ttcttttacc tcaatttttg ccgcaacttg gcaatttttt cgatcacctt
     2161 cttacctccg atatagtaca gcaaacatat caaatgcaca acagcacagt gcacttgtgc
     2221 aatcaatggc tatgctgtag aatgcactca tgcacagtgc aggtgtgcaa aataaatgat
     2281 aatttggagg ttgcatggct cttgttcgct caggaaacaa aagcaagatt tttgaagcag
     2341 atacgaagtt taactcggtt tttgacactg aatataagcc gggagaaaca tgtgaatatg
     2401 aagggattta taaatgtacc ggttgcggta aagaagtaac tatcgcaggc gggaaaacaa
     2461 tcccaccaca aaaccaccac caacataccc ccgcgcaagg gaacattcgt tggcaattgt
     2521 ttgtttatgc tcagtgaggt atagaaaatg gcagatcgtc gaccactcaa cccacacgtt
     2581 gtcgttccta aaccaggcgg cggaactcaa cctcgtgacc gtaacaatga cggaagggtc
     2641 agaggaaaac gctcagatgc aggaaagcca cgaaaataac agcaagccgc cagcctggcg
     2701 gcttttttat atctgcagct gatactgatt taccataaca tccgttgggc gcaccgctga
     2761 aacggggttg gctgcgtttc cggtgccaga cgcgagaaag tggcgacaga cataacgaaa
     2821 aaacaccaga aacgcagccg ctgaatatta cactggtgca tagtcatgca gccagatgca
     2881 tggatgttaa agtcacacta aaatccgcgc caatgcctta aaaaccgaca aatgcttctc
     2941 cagccagtcc cacagctccg ccgccagata caggcagtta agcaatgatt caatgagatt
     3001 taacagcttc atttcccgct cctgaataag tcgtgacctt tttgatcacg aacctattta
     3061 gcgggagaaa taagagcccc tcttcgtgcg ggttgtcagc tgcctggatg gtttaaagtg
     3121 tcggcccgcg gtaggttttc tgcggctggc gctccagctc cagtgttttc tgatactcat
     3181 tctccctctg ccggagctcg atggcattca gttcccggac aagcggcgca gctgctcgct
     3241 taaactcagc acctgcgtgc tcaagtacga cacgcgctcg ttcagcaccg cgttctcccg
     3301 ttgcataagc ccacacatgc tccgccattc cgcgaaggcg ctctcccatt cgtccagcct
     3361 ttgcgagtag tcctgctgta gctgctctaa tgcgctcagc aactgctttt ccagctctgt
     3421 cattttccgc tactcctgtt tcaggcggat ccatgtcgtc ccgtcgtaag ggatggcttc
     3481 ggtttgcatc cccggcggca ggataagaaa cctccggccg tcgctgctct cctgatagcg
     3541 cactccccac gtccgggcgg tcattttcgc cagggtatct tcctgctgac gaagatgcgt
     3601 gtaattgtcg gttatctgct gcccctgcca ccacagtatg ctcccgctcg tgccgatcag
     3661 cagcgctgac accatcagga tggtgagcca tgtccgcccg gcagtccgca gtaagctccg
     3721 ccggtttttc tccatcgcct cgctcatact ctgctcgtgg gcgaggatgg cgtcgctgat
     3781 tctcctcgcg cttgcgctca gttccgctct gacagactgc tcgtgctcgc tgaatgcggc
     3841 tttcagcatc tcgccggtac tctgctgctg cgctttcgat tgctgctcta agtccttcgc
     3901 cagcgttaaa agactgctca tagatggctc ccttcagtcg gatgtttcgc cccccgtccg
     3961 ggtcggcaat gctgatactg cttttcgtgg tgcgcaccac ctcaaaacct gaccgttcca
     4021 gcgcctcagt gacgtcctga cggtttttca gctccccgga cgaagcaagg gtcagcagtc
     4081 cgcgcgtgat ggcttcagtg gcctcctgct tcgttttcgg cagtccggac ggcgtgacca
     4141 gcgcccggcg gttttccggc gcgttcgggt catgcagccc cagtctgccg ttcacgatgg
     4201 tctgccaggc atcgatgcgc ggccggtcgg cgcggtcgta gtacggctgg agccgcctgc
     4261 cggtcagcag ttcggtattg gggatcagaa agttcagctc cagacgcccc ttgtccgcgt
     4321 gctccaccca cagaatgctg tactgatctt tatcgagtcc gggcatcaga acccgctcga
     4381 agctggccat cagtttttcc cgctggccgg gaggcagatc ggcctccgca aacgacagga
     4441 cgccggaggt gtatttcttc acgtaaggcg acgcgtcaat cagctcccgg acttcgtccg
     4501 gcttcccctg caggacgctg gcgccttcac gctgccggtc tttacccagc agatagtcca
     4561 ccggcccggc gccaccgccg cgaccgcgtg gatgaaattt aacgatcatc gtccgttcct
     4621 ttctcaagaa cggcgtgccg cagccgctca agcccggcat cgatggccat cagcgccgcc
     4681 acaatctgta ccctgtcatg gccggtgccg ccgccggcgt taacgcggcg ggctatctgg
     4741 ttcaggttat tgcccatgcc ggcaagctgg cgcagcagcg ccg
//
