LOCUS       NZ_CP056172             4804 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter roggenkampii strain RHBSTW-00695 plasmid
            pRHBSTW-00695_5, complete sequence.
ACCESSION   NZ_CP056172
VERSION     NZ_CP056172.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148685
            Assembly: GCF_013733395.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.5654070765511x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013733395.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:14:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,933
            CDSs (total)                      :: 4,818
            Genes (coding)                    :: 4,681
            CDSs (with protein)               :: 4,681
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 45 of 137
            Pseudo Genes (incomplete)         :: 74 of 137
            Pseudo Genes (internal stop)      :: 29 of 137
            Pseudo Genes (multiple problems)  :: 12 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056172.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00695"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:1812935"
                     /plasmid="pRHBSTW-00695_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1331..1900
                     /locus_tag="HV268_RS24525"
                     /old_locus_tag="HV268_24530"
     CDS             1331..1900
                     /locus_tag="HV268_RS24525"
                     /old_locus_tag="HV268_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310049.1"
                     /translation="MAKLPLPLPIASPPTVSNSGSERAKKSGRRTLSAKRKTLLEELWG
                     DELEQLKVWNRHEHDGYTTVPRTLTYISRILDYLGGTGLPLSQTYLALWCRVFDEGFVE
                     IRDKDGFAYEAGFSGQRAVTTWTGRMRKLRDLGFITTKPGTSGEFQYVILLNPLTVIKE
                     LYEGKEKDERYNALVGRMQEVGAKWE"
                     /name="hypothetical protein"
     gene            1904..2449
                     /locus_tag="HV268_RS24530"
                     /old_locus_tag="HV268_24535"
     CDS             1904..2449
                     /locus_tag="HV268_RS24530"
                     /old_locus_tag="HV268_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010450637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009310050.1"
                     /translation="MASYFPTLDISGEEFDFTHLEPFIMHVNSDMAKKVLKVHVRFTNH
                     CFTKGYEAFYHPAGEQIIYDHSGNGRTFCPIRYRLSLALPEIINRLCDPAEKVYQTSAK
                     RNWAYSITIDDPEGPYHLFFEIRRASRDEKHLQDLNMVIESAYPEDRGYGPPSLLGRIG
                     FVGLCGRVFCGKPLATKR"
                     /name="hypothetical protein"
     gene            3153..3362
                     /locus_tag="HV268_RS24535"
                     /old_locus_tag="HV268_24540"
     CDS             3153..3362
                     /locus_tag="HV268_RS24535"
                     /old_locus_tag="HV268_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(4204..4605)
                     /locus_tag="HV268_RS24540"
                     /old_locus_tag="HV268_24545"
     CDS             complement(4204..4605)
                     /locus_tag="HV268_RS24540"
                     /old_locus_tag="HV268_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4605..4796)
                     /locus_tag="HV268_RS24545"
                     /old_locus_tag="HV268_24550"
     CDS             complement(4605..4796)
                     /locus_tag="HV268_RS24545"
                     /old_locus_tag="HV268_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          41..511
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.889"
                     /coverage="91.0"
                     /evalue="4.61e-163"
ORIGIN
        1 tcacagcgtc agtgctcagt gtagggatgt tctcttttcc gcttcctcgc tcactcagtc
       61 gctacgctcc tgccgttcgg ctgcggcgag cggcaggagc atgcgtacaa aagctcgaga
      121 aaaccgagca atcgcgtgat ggggaaaggc cgcggcgttg ccgtttttcc ataggctccg
      181 cccccctgac aagcatcaca gaatctgacg ctcaaatcag tggtggcgaa acccgacagg
      241 actataaaga taccaggcgt ttccccctga tggctccctc gtgcgctctc ctgttcctgc
      301 cattcggttt gccggtgtca ttccgctgtt atggccgcgt ttatctcatt ccacgccaga
      361 cactcagttc cgagaaggca gttcgctcca agctggactg tctgcacgaa ccccccgttc
      421 agtccgaccg ctgcgcctta accggtaact atcatcttga gtccaacccg gaaagacacg
      481 acaaagcgcc actggcagca gccattggta agagtcaatg tgcgagagat tttagagagt
      541 acttgaagtg atggccggac tacagctaca ctgaaagaac agttttggtg actcgtctcg
      601 cacaagacag ttacctggtt aagcagttcc ccaactaact taaccttcga tcaaaccacc
      661 tccccaggtg gttttttcgt tttcagagca agagattacg acgatacaaa tgggatctca
      721 agaagatcct taactaattg cagctcgctt cactatcaca ttcaactccc gtcaccgtcg
      781 attaaaacca tatacaacta cggtcaggtg attccgctca ctggagcgaa attacccgtt
      841 aagctcaaaa cgcttaacgt aggataattc ccgttttcca agctaccccc atcaacatcc
      901 acacatagct ctcagacgcg ctcaggcaag agccaagtca tttcctgccc caaacctcac
      961 cataaccata aaaaccaccg aaaatcgaac acagggcact atgagcaggc atatcgattt
     1021 gcattaaatg gctgccgtaa aattattacc ttgccccctt gcatccccat tcagcaccgc
     1081 gagcacattc agcacattga gcacaattaa gcacagcgag cacagaaacc tgtcataact
     1141 cgttgaatat cagtcaccat atgctgtatt tattacaact cacagattga gttgtaataa
     1201 atacatactc acaagatacc tatacgttga aaggcaacat cacaaccaca cgcaaaacac
     1261 aacataactc attgttttaa aagaaaaaac acttaaaata accgtatgtt aaatgatttt
     1321 ggagaatata atggcgaagt taccactgcc actaccgata gcgtcccccc caacagtaag
     1381 caatagtggc tcagaaagag cgaagaagtc aggaagaaga actctttcag caaaaaggaa
     1441 aaccttgtta gaagaactat ggggagacga acttgaacaa ctcaaggtat ggaatcgcca
     1501 tgagcatgac gggtatacaa ctgtgccaag aacgctgaca tatatcagtc gaattctcga
     1561 ttatctcgga ggaacgggac tgccattgtc ccaaacatat ctggctcttt ggtgcagggt
     1621 atttgatgag ggctttgtgg aaataagaga taaagatgga tttgcttatg aagctggttt
     1681 tagtggacag cgagcagtaa ccacatggac agggaggatg agaaaactaa gagatttagg
     1741 tttcataact acaaaacctg gcacatcagg tgagtttcaa tatgtgattt tgcttaaccc
     1801 actgactgtc attaaggagt tatacgaagg taaggaaaaa gatgaaagat acaatgcatt
     1861 agtgggccgt atgcaggagg ttggtgctaa atgggagtaa ttcatggcta gctatttccc
     1921 gacgttagac atatctggag aagagttcga tttcactcac cttgagccat tcattatgca
     1981 cgtaaatagc gatatggcta aaaaggtatt aaaagtacac gttaggttca caaatcattg
     2041 ttttacaaaa ggttatgaag ctttttatca cccagcagga gaacagataa tttacgacca
     2101 tagcgggaat gggagaacgt tttgcccaat tagatataga ttatctttgg cattacctga
     2161 gattatcaac cgtctatgcg atccagcaga aaaggtgtat caaacatcag caaagagaaa
     2221 ctgggcctac tcgattacga ttgatgatcc agaggggcca tatcacttat tttttgaaat
     2281 aagacgcgcc tcgcgtgatg agaaacacct acaagatttg aacatggtta tcgagagtgc
     2341 ctatcctgaa gatcgagggt acgggcctcc gtccttacta gggcgaattg gctttgtagg
     2401 gttatgcggt agggtatttt gtgggaagcc tctggcgact aaaagatgaa aaaaaagcac
     2461 ctgaaaggtg ccttttttct gtttctttgg taatcgttcc ggacaccact agcttttgct
     2521 taggttcgcc ttgcgcgaac acaggaacaa gatttgtcct gctgggtagc gctcaatctc
     2581 atccggtcag tcgctacgat gtaaacaata taacaaaacc aataacgcaa ttcagcacca
     2641 ccggttcaat gcaatgcttt gattcatatg gagcaccatg agtacagaaa ccataagtaa
     2701 ccatctgaat ttaaatggca agatgcattt tataaaacaa ctcaacttct gagttgtttt
     2761 ataaaatgca tctgttctta tgattcacca tgatttcact acaatagaaa aaaacataga
     2821 tagttgtttt catttaaaaa ataaatcccc tcctgttttc atcaccttga tgcattggca
     2881 atcgtcgcac ggcagctttc actgataccc tcagcctgac ggcagcggtc ggtgtaattg
     2941 tcgcctaata attgtacgaa ccctcgaggc tattcatcct gcccctaaat cagagtctca
     3001 cacctggttt tgatgcgctc aatttttgtt ctattttttt gaatgatatc cgcaaattgt
     3061 ttcgatttta atcagttcat ggtcgtccta tcatttatca catcagggca acaacgcccc
     3121 atacagaaca aaatgactta aggaatacga ccatgaaatc catcaaaact ttcgttgcag
     3181 ttgccgctct atccatgatg tctttcggtg cctttgctca gagcgtcagc gctaccgcct
     3241 ccaccctgga tcgtgctgaa gcaaaaattg ccgcacaggc cgctgaacaa ggtgcgtcat
     3301 acaaaatcac cagcgctcag ttcaataacc gtgtccacat gacggctgaa ctgaccaaat
     3361 aagatggtcg cagagggagc tgccgaacag gtcgccggga ggcggcccgt ttcgtccctt
     3421 agcaggaaaa ccttcaggcc ccttcggggc ttttttgcat ctgtcattta acataaaggc
     3481 atgattgtac gcaccgctaa aacggcctca gggcgtttcc gacgggaaac cggtaaaaca
     3541 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     3601 gagtatgcac gcaagtgcat agcgcgaaaa ttcacgccat tcagccaggg taagtcctgt
     3661 ttccggcgtt cttcctatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     3721 aagcgttgcc ggcgcagccg gaaaaccggt tttcagcaca ctcaggaatg gggttcgtcg
     3781 acggccggca atgacgttat ggcaagtcgc cggacgactc accacacgat tcaccggcga
     3841 ttcgactcgt gacttgactc tagagtcgcc aaggtcaaaa aaacaccaaa atgacgattc
     3901 gactctagag tcaagtcgtc ataagttact gatagtctta agtgagtcgt cgatgactct
     3961 atgacgagtc gtcggcaagt cgtacacgaa tcgccgacga cttggattca ccttacagaa
     4021 agtaatactg cgtaatttaa taacccacaa ctaactgata ttaatgagtt atcttcaaaa
     4081 ttttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctgacg
     4141 atttttggga aggttcagga actccccgga atgaccggag aggaaaactg aagcatgacg
     4201 gggtcaggat tttttccaga atttccacca tggcgccggg gattcagtta attttgcctg
     4261 gtgattttca agaaggagca tggcctgttt caggctgtca atgtgcgaat ccttggacaa
     4321 aagtaaggtg ttcaggtgtt caatctcctg tttcagatga cgaatcacct gttcactctg
     4381 gtcagcagag gctgtatcac gctgttcaga gacctgtcca gaatggtgtt cacccttcgg
     4441 ttgctcaaag ggaccaaaga cacgtaaaag ctctgaagta tctaccattt ttgcattacg
     4501 ttcaccgcta caggtggaca ggtcacctgc tttaattaac ctgtgcagtg ttgttctact
     4561 cttacccgtc agtcgtgcag cctcactaat agacactctg gccatcagtt accatcctgt
     4621 aatgcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc
     4681 tcacaaaggt cggcctcgac atcgagatca agctcgttaa gcttctccag caggagcaga
     4741 gactgattct ggatgaattt tgccatattc agcgctgttt tctgttgttt cgtcataaga
     4801 ggcc
//
