LOCUS       NZ_CP056158             2494 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00844 plasmid pRHBSTW-00844_4,
            complete sequence.
ACCESSION   NZ_CP056158
VERSION     NZ_CP056158.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148712
            Assembly: GCF_013731155.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00844
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 172.815471028129x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013731155.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:47:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,746
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,531
            CDSs (with protein)               :: 4,531
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 31 of 100
            Pseudo Genes (incomplete)         :: 60 of 100
            Pseudo Genes (internal stop)      :: 25 of 100
            Pseudo Genes (multiple problems)  :: 14 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056158.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00844"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00844_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            160..345
                     /locus_tag="HV295_RS23630"
                     /old_locus_tag="HV295_23625"
     CDS             160..345
                     /locus_tag="HV295_RS23630"
                     /old_locus_tag="HV295_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(636..854)
                     /locus_tag="HV295_RS23635"
                     /old_locus_tag="HV295_23630"
     CDS             complement(636..854)
                     /locus_tag="HV295_RS23635"
                     /old_locus_tag="HV295_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            2061..2288
                     /locus_tag="HV295_RS23640"
                     /old_locus_tag="HV295_23635"
     CDS             2061..2288
                     /locus_tag="HV295_RS23640"
                     /old_locus_tag="HV295_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(393..480)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1118..1646
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 agcgcgttcc tgacgaaacc atgacaaaaa ccacaacaaa ccaccccgga acccgtcaga
       61 aacgcgcctg ttaaatttta acggcatgca tgactatgca ccagaatgac gccatgctct
      121 tttcacgcaa aaatcatcac cagacgggga aaatcaccag tgaccagaca ggaatccgcc
      181 gccctcaata tggccaaatt tatccgcgca cagacacttc tcctccttga gcggctcgag
      241 cagatggatc tggatgaggc tgccggctgc tgtgagcacc tgcacgatca ggccgaagcg
      301 ctttacgcca tgctgaacgc acagataggc gaggaaaatg cgtgaaaatc ggtgaacggg
      361 tgcgcaattc agtgcgcggc cgtgaggcga tggcggggtg tcggggcgca gccctgacca
      421 gggtatttgt gatgccggcg cgtgcgcggt attacaaatg cacatcctgt cccggaacgg
      481 acaccgggaa acagcaaaaa aaaccgggcg gcacgcccgg aactcaatca agttagatta
      541 gattactctc actcgtccat aacagcatca tggaacgacg accaccgtcc gtgacggccg
      601 cctcgtttaa gtatggacag aaatacagaa aatgctcagg acgaaatgta atgaatgcga
      661 acggattcaa gaaattcgag catgacagtc cttacggccg gttcggtttc agacaaaatc
      721 tgccggtatg catccagcat catggctccg gcatcccctc cggcacgccg tagccagacc
      781 gaaacaacgg acacaagcag gtgtcgctca tcatcactaa gagtcatcag ggctccggaa
      841 gaaaaaccaa acatggctta cctactgtta acggcaacgc agaagaaagg tttacagggt
      901 agcgggcagg gatgaaaaaa aaaaattgca gtcaggccgg aacggctgct gaaccggtac
      961 aggcgggttc cgggcgcagc gccggaccag tcacggagca ctagcggagt gtatactggc
     1021 ttaagatgta gcatggcagt aacacatagc attaatgcca cataagacag gcgcagaaag
     1081 tgaaagaaaa cgcgtcagga gaaaatgtga tacagaatat attccgcttc ctcgctcact
     1141 gactcgctcc gctcggtcgt tcggctgcgg cgagcgggac gggcttacgg atggatcaga
     1201 aatttcctgg gggatgccag gaagatactt aacgaagaaa gcataaggcc gcgccaaagc
     1261 cgtttttcca taggctccgc ccccctgaca agcatcacag aatctgacgc tcaaatcagt
     1321 ggtggcgaaa cccgatgcgg cttaaagata ccaggcgttt ccccctggtg gctccctcgt
     1381 gctcccccgc attcctgcct ttcggtttac cggtgtcact ccgctgttat ggccgcggtt
     1441 tctcattcca cgcctgacac tcagttccgg gaaggcagtt cgctccagga tggactgtat
     1501 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc aacttgagtc
     1561 caacccggaa agacacgact aatcgccatt ggcaacggcc attggcaaac aggatgtgcg
     1621 agagatgtct caagagttct tgaagtggcg gccttagtga ggctacactg aaaggacaaa
     1681 tttggtgaca tgtctcgcac aagactgtta ccacggttag gggatctgcc aggattacct
     1741 aaccttcgaa aaaccacctg ccagggtggt tttttcgttt acagggcaag agattacgcg
     1801 cggaaaaaaa ggatctcaag aagatccttt ataaattaga catccaacct cacaaagagg
     1861 aatacttaaa tcatcttggt atatgtatcg gccggattac atctcatata agcctcctcc
     1921 ccgcctgaca ccacactttg accatagcac ctcgcgccta actcgatata cagcccacca
     1981 tcaagcctct tttcacataa aaaatgacct atagttagat ggttagtgac caaaactgat
     2041 atggataaaa ggagctggct ttgaaaaaga tttttgtttc agtgttcgca gcggcagttg
     2101 cgctttcagc cctcaccggc tgcacgcgca caagctatgc catacacacc aatgacggtc
     2161 gcactattgt cagcgacggc aaacctaaag aatcagactc aggcctgctt ggctatacag
     2221 acgcgaatgg tgtcaaacag cagatcaata aaaccgacgt taaagaagtc tccgaaattc
     2281 cgcactgagc atcttctccg aaaaaggccg ctaatgtggc ctttttcttt acctgtggtt
     2341 ctccgccaaa atcccagcaa attgcatcac caaagctaaa agctttcagg gttgtctaaa
     2401 aagcacaaga cataagagga agtgcggtat tttataatca agcccccaag aatttaccat
     2461 aacatccgtt gcccgcaccg cctgagacgc gttc
//
