LOCUS       NZ_CP056153             3662 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00853 plasmid pRHBSTW-00853_4,
            complete sequence.
ACCESSION   NZ_CP056153
VERSION     NZ_CP056153.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148716
            Assembly: GCF_013730235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00853
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 134.023672755191x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013730235.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 05:13:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,069
            CDSs (total)                      :: 5,950
            Genes (coding)                    :: 5,780
            CDSs (with protein)               :: 5,780
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 56 of 170
            Pseudo Genes (incomplete)         :: 125 of 170
            Pseudo Genes (internal stop)      :: 34 of 170
            Pseudo Genes (multiple problems)  :: 38 of 170
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056153.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00853"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00853_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            200..493
                     /locus_tag="HV299_RS30135"
                     /old_locus_tag="HV299_30150"
     CDS             200..493
                     /locus_tag="HV299_RS30135"
                     /old_locus_tag="HV299_30150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023317864.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLDVSQSTLSRLLN
                     GKTDLSYEMALRLSKVVGRSAESWVNMQTQFSLSKSRKSLAAELEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            557..859
                     /locus_tag="HV299_RS30140"
                     /old_locus_tag="HV299_30155"
     CDS             557..859
                     /locus_tag="HV299_RS30140"
                     /old_locus_tag="HV299_30155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="WP_023317863.1"
                     /translation="MVDFYISAIKMDSAGQHIEFVKVHKINRDGSWNTAGSISSRKFIS
                     ELISMGKASFMTVVKNSSTGKWNLGAKVEAMNDGYITTDPNKTKLDNLGNLPKFV"
                     /name="DUF3892 domain-containing protein"
     gene            complement(1643..2044)
                     /locus_tag="HV299_RS30145"
                     /old_locus_tag="HV299_30160"
     CDS             complement(1643..2044)
                     /locus_tag="HV299_RS30145"
                     /old_locus_tag="HV299_30160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_181500098.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSICSGERNAKMIDTSE
                     LLRVFGPFEHPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2044..2235)
                     /locus_tag="HV299_RS30150"
                     /old_locus_tag="HV299_30165"
     CDS             complement(2044..2235)
                     /locus_tag="HV299_RS30150"
                     /old_locus_tag="HV299_30165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_181500097.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELELDAEAGLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2640..2786
                     /locus_tag="HV299_RS30515"
     CDS             2640..2786
                     /locus_tag="HV299_RS30515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073545459.1"
                     /translation="MHEPPVQSDCCTLSGNYHLESNPVRHAQAPLAAATGKKVYKRLLI
                     QSS"
                     /name="replication initiation protein"
     gene            complement(3244..3525)
                     /locus_tag="HV299_RS30155"
                     /old_locus_tag="HV299_30170"
     CDS             complement(3244..3525)
                     /locus_tag="HV299_RS30155"
                     /old_locus_tag="HV299_30170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032440460.1"
                     /translation="MIKNIKHKGLRSFAEEGSTAGIQPSMAKRLRMILTTLSTATCIQD
                     VVDVRALGCHPLKGERKGEYSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          2377..2749
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.933"
                     /coverage="91.0"
                     /evalue="2.7800000000000003e-164"
ORIGIN
        1 caacgaatcc taagggtaaa atactgcgcc ttgaaaaggc cataaaagcc catacatacg
       61 cagtagcttt gactccaacg agtatcctaa agctgactgc tttttttgtc aatatcatga
      121 acttgcacat tgtgcaatgc gcatactgaa aggcagtaat ttttttgtat aatcaaatac
      181 aaaacaaaca gaggaaggca tgataaatct ttacgatcct gttcatcctg gtgagtttat
      241 cagagaaatg ttcatggaac cgttcgaaat acctgccgca gagttagccg ataaacttga
      301 tgtatcccag tctaccttaa gtagactgtt aaatgggaaa acagacttaa gctatgaaat
      361 ggcacttaga ttgtcgaagg tcgtaggccg ctctgcagag tcgtgggtaa acatgcagac
      421 ccaattcagc ctgtcaaaaa gtcgcaaatc tttagcggca gagctggaac acttgcatcc
      481 aatgtttcca tgaaaaaagc ccgcagaacg cgggcttttg ttacgtaaaa cacccagcag
      541 ccatcaggag gcaaccatgg tagatttcta tatctcagca atcaaaatgg acagtgctgg
      601 tcagcacatc gaattcgtaa aagttcataa aataaaccga gatggctcat ggaacactgc
      661 cggctcaatc agctctcgca aattcatcag cgagctgatc agcatgggaa aagccagctt
      721 tatgacagtt gtaaagaaca gctctactgg taagtggaac ctaggagcca aagtggaagc
      781 gatgaacgat ggctacataa ccacagatcc aaacaaaact aaactcgata acttgggaaa
      841 tctgccgaaa tttgtctgat atgcaataac atccactaag ttccagcaga atcccctccc
      901 taatgattta acataaagtc ataattgtac gcaccgctaa aacggcctca gggcgtttct
      961 gacaggaaac cggcaaaaca ggcaacaaac cacccgaaaa cccgccagaa acacgcaggc
     1021 tctgttttta cggcatgcat gagtatgcac acaagtgcat agcgcgaaaa ttcacaccat
     1081 tcagccaggg taagtcctgt ttccggcgtc cttctgatcc gaaaacggtg aacggggacg
     1141 caatttattg cgtggccgtg aaacgttgcc ggcgcagccg gaaaaccggt tttcagcaca
     1201 ctcaggaatc gggttcgtcg acggccggca atgacgttat ggcaagtcgc cggacgactc
     1261 accacaagat tcgccggcga ttcgactcgt gacttgactc tagagtcatc aaggtcaaaa
     1321 aacaccaaaa tgacgattcg actctagagt caagtcgtca taagttactg atagtcttaa
     1381 gtgagtcgtc gatgactcta tgacgagtcg tcggcaagtc gtacacgaat cgccgacgac
     1441 ttggattcac cttacagaaa gtaatactgc gtaatttaat aacccacaac taactgatat
     1501 taatgggtta tcttcaaaat tttaattttg cgtggggtgt gggtattttt agtggtgagg
     1561 agcgttcaga gatctgacga tttttgggaa ggttcaggaa ctccccggaa tgaccggaga
     1621 ggaaaactga agcatgacgc ggtcaggatt ttttccagaa tttccaccat ggcgccgggg
     1681 attcagttaa ttttgcctgg tgattttcaa gaaggagcat ggcctgtttc aggctgtcaa
     1741 tgtgcgaatc cttggacaaa agtaaggtgt tcaggtgttc aatctcctgt ttcagatgac
     1801 gaattacctg ttcactctgg tcagcagagg ctgtatcacg ctgttcagag acctgtccag
     1861 aatggtgttc acccttcggg tgttcaaagg ggccaaagac acgtaaaagc tctgaagtat
     1921 ctatcatttt tgcattacgt tcaccgctac agatggacag atcacctgct ttaattaacc
     1981 tgtgcagtgt tgttctactc ttacccgtca gtcgggcagc ctcactaata gacactctgg
     2041 ccatcagtta ccatcctgta atgcatccag tctcgaggac agtgtacgga aaagatgctc
     2101 cgcatcatcg tggagtttct cacaaaggcc ggcctcagca tcaagctcaa gctcgttaag
     2161 cttctccagc aggagcagag actgatgctg gatgaattta gccatgttca gcgctgtttt
     2221 ctgttgttta gtcataagga acctcagagc gtcagtgctc agtgtaaaac cagtctcttt
     2281 tccgcttcct cgctcactca gtcgctacgc tcctgccgtt cggctgcggc gagcggtcgc
     2341 aatacgcaca taacgaaata ggacagacag cacggagagg gagaaggccg cacaccggcc
     2401 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     2461 gtggcgaacc ccgacaggac tataaagata ccaggcgttt ccccctggtg gctccctcgt
     2521 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     2581 atctcattcc acgccagaca ctcagttccg agtaggcagt tcgctccaag ctggactgta
     2641 tgcacgaacc ccccgttcag tccgactgct gcaccttatc cggtaactat catcttgagt
     2701 ccaacccggt aagacacgca caagcgccac tggcagcagc cactggtaag aaggtgtaca
     2761 agagattact gatacagagt tcttgaagtc tcagcccaac tacggctaaa ctgaaaggac
     2821 agaatttggt ttcagcggtc ttgtacaccc tgttaccacg gttcagaagt tccccaactt
     2881 actgaaccct cgaaaaaacc gcctccccag gcggtttttt cgtctcggcg agtaagatac
     2941 gctttatcat tcaaagcagt caaggaagaa ccaagacagc tcactcacca gcaggctcct
     3001 ttttattggt agacacatgc ttctggacag tatcagcccc tgcacaaagt acagcagcta
     3061 acaagaacca tccccagcca tcaataccac gaaaagctag gactccagca atagttacgc
     3121 taatcaacgg gaacaataaa accataaagg atctcatact ttctccagag gttacttttt
     3181 taataaagtc cctaatccat ccgtactttc acttggatgg attagcaact tacttattga
     3241 taactaatga taatcttcaa gatcaacgtc taagacgttt ggttcctcaa ccttgaaaac
     3301 tacccgccag ttcccattaa cttcaacgga atactcgcct ttacgctccc cctttagggg
     3361 gtggcaccct aacgccctaa cgtcaacaac gtcttgaatg caagttgctg tgcttagcgt
     3421 agtcaaaatc atgcgaagtc ttttcgccat actgggctga ataccggcgg tgctaccttc
     3481 ttcagcaaag ctcctaagtc ctttatgttt tatgtttttt atcatctaca atccccatat
     3541 ttttttcgtc tatacctgta tctatatttt aaaatcattt taattagttc cattttgccc
     3601 ccttgcttta ttaaaacaat tgacgtaagt ttgttcttga ggtcaatcac ctcatttgac
     3661 tt
//
