LOCUS       NZ_CP056152             4667 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00853 plasmid pRHBSTW-00853_3,
            complete sequence.
ACCESSION   NZ_CP056152
VERSION     NZ_CP056152.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148716
            Assembly: GCF_013730235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00853
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 134.023672755191x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013730235.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 05:13:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,069
            CDSs (total)                      :: 5,950
            Genes (coding)                    :: 5,780
            CDSs (with protein)               :: 5,780
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 56 of 170
            Pseudo Genes (incomplete)         :: 125 of 170
            Pseudo Genes (internal stop)      :: 34 of 170
            Pseudo Genes (multiple problems)  :: 38 of 170
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056152.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00853"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00853_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            308..1864
                     /locus_tag="HV299_RS30125"
                     /old_locus_tag="HV299_30125"
     CDS             308..1864
                     /locus_tag="HV299_RS30125"
                     /old_locus_tag="HV299_30125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_045326453.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            2924..3247
                     /locus_tag="HV299_RS30130"
                     /old_locus_tag="HV299_30130"
     CDS             2924..3247
                     /locus_tag="HV299_RS30130"
                     /old_locus_tag="HV299_30130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3456..>3890
                     /locus_tag="HV299_RS30565"
                     /pseudo=""
     CDS             3456..>3890
                     /locus_tag="HV299_RS30565"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3925..4410
                     /locus_tag="HV299_RS30205"
                     /old_locus_tag="HV299_30140"
     CDS             3925..4410
                     /locus_tag="HV299_RS30205"
                     /old_locus_tag="HV299_30140"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            4414..4629
                     /locus_tag="HV299_RS30210"
                     /old_locus_tag="HV299_30145"
     CDS             4414..4629
                     /locus_tag="HV299_RS30210"
                     /old_locus_tag="HV299_30145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2639..2811
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(2162..2714)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2925..3241
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          3238..4661
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.0"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 cagcatcaga aagcgctgga gctggagcga tctcagcgac agcggacgta taacagccct
       61 tcactgtgat aacctgataa aacggggctt tcggtggaca tctgcgctga aagccggagc
      121 cattcacccg actgcatagc catgcagccg ggtgaaattt atctggcgca gaatgcgcgg
      181 tttagcggag agtttgctgc cggttttacc gggctgacgc cgcatttgtt ccgaacccgt
      241 ccggggcggt gctgataagg gatgttatgg taaatgcggc attttttatt atatggagtg
      301 tatttttatg agaattaaaa agcctcacct tcgtccgata gtcataacct tgtccgttgg
      361 tgggattttg ttaacttcta tttttctgat tgccgttatt atggtttttc agcgcgaaaa
      421 tatagaaaaa gatttgcttg atgctaattc atcgtacgct atgaaaatgt cagatgtaat
      481 gagtggttat attgaaatgg ctcaaggtga attagcatat ggagcaaaaa aaataaaatc
      541 aactaatgat attgactcac ttaaagatga ggcggatagg ttaagattgc agtctggtat
      601 gtttaattca gtttttgttg ttagtaatga tgctgttgtt ctggcgactt cgccagaatc
      661 tcttgattta gttggtaccc gcttgaattc aagtgccagc aaattggcga ttgaggaaaa
      721 gaaaccattc atatcttcac cgttcaaatc agttactggg aatttgatta ttttgttgtc
      781 acatcctatt tatgataaga atggcaatta catagggcat gtcggtgggt ctatatatct
      841 aaaaaaacat agtttattta gcgatgtttt gagcaggcat tttttcaaga gcgacactga
      901 aattagcatt gttagtgatg atggaactgt gatctttaat aacgataaca gtgctgttgg
      961 tcggcccatg gttatgtctg atgatttgaa atcaaagtta acggagtctg atagaggtga
     1021 gggggaattt tttttttcgg ggaatagata tcttctgggt tatgctcata tgaaaaatac
     1081 tgcttggaat gtgttggttt atagccatgc tgacagcgcg actactattc ttattactta
     1141 tgctaaaagt gtacttgttc tgctaattgt aataattatt acattttcac ttctttcata
     1201 tttcattgcc tcccgtattt cacttccttt agaaaagctt gctatatcta ccaatgctaa
     1261 agatataggg gcttcgttag tttatattaa agctatcaat gcttggtatg ccgaagctga
     1321 tcgacttaaa aaggcacttt ttacgcatgt taaggtaatg atggaaaggg ttgattcttt
     1381 aaatgaggtg gcgcttaaag atccccttac cggcgtgaac aatagattgg ggttttcaac
     1441 taaaatacag aataacaagt atggggcagg agcttcagtt attgctattg atgttgattt
     1501 ttttaaaaaa ataaatgata gtttcgggca tggggtcgga gatgaggtgc ttgtttcttt
     1561 ggctcagatt attaagtctt gttgccgtag tgaagatatt atttgtcggt ttggtggaga
     1621 agagtttgtt gtttttctgc cgaatacatc tgtggtaaca gcggagtgtg ttgcggagcg
     1681 tattaggagt gttattgaac gtactgtttt ccctaataac ttgcgtgtca ccgtatctgc
     1741 tggtgtggca acacaagatg atcctcttgg tgggatcgat tttttgctga aaaatgctga
     1801 cgatgctctt tatcaagcta agagagaggg gcgtaataaa gtgattgtat atagcgctat
     1861 ttgagttttt ttgatgacgc tttacgcgct agtgtgccgt ttttttttgt ggggaggggg
     1921 gttcaagaag cggatcctct tgttatccct tcgatgtcgt cgtaatctca tgccctgcaa
     1981 acaaaaaaac caccgctacc aacggtggtt cgtttgccgg aatcaagagc taccaactct
     2041 ttcttccgaa ggtaactggc tttcacggag cgcaggtacc aaaactgtcc ttccagtgta
     2101 gccgcagtca ggccaccact tcaagaactc tgtagaacac tctactactc cgctccgtga
     2161 atcctcttac taatggctac cgccactggc gatttttcgt gtctgttccg ggttggactc
     2221 aagatgatag ttaccgggac aacgtggtag tcgggctgaa cggggggttc gtgcatacag
     2281 tccagcttgg agcgaactgc cttcccggaa ccgagtgtca ggcgtggaat gagataaacg
     2341 cggccataac agcggattga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
     2401 ggagccacca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg
     2461 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
     2521 cgccgtggcc ttatcgcttc cctgttaagc ttcttcctgg catctcccag gaaatttccg
     2581 ccccgcctgt aagccggtac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2641 agcgaggaag cggaatatat cttgtatcac atattctgct gacgctccgc tgctgcattt
     2701 ttctcctgcc acatgaagca tttcactgac actcgcatcc gtgccaacat agtaagccag
     2761 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac ccgccaaaag
     2821 cctgacgcgc ctgcggcttg tccaaccccg cgccgaccgg gaaaggcttt cccgcccgtc
     2881 ccggcgttat caggaggccg gtttgtcaga gaaacggaac aagatgctca ccatgtgggt
     2941 gacggaggac gagcaccggc ggctgcttga gcgctgcgac ggcaggcagc tggcagcgtg
     3001 gatgcggcag acctgcctgg acgaaaagcc ggcacgttcc ggaaagcttc cctcaatctc
     3061 gccggcgctg ctgcgccagc ttgccggcat gggcaataac ctgaaccaga tagcccgccg
     3121 tgttaacgcc ggcggcggta ccggccatga cagggtgcag attgttgcgg cgctgatggc
     3181 catcgatgcc ggacttgagc ggctgcggca cgccgttctt gagaaaggaa cggacgatga
     3241 tcgttaaatt tcatccacgc ggtcgcggcg gtggcgccgg gccggtggac tatctgctgg
     3301 gtaaagaccg gcagcgtgaa ggcgccaccg tcctgcaggg gaagccggaa gaagtccggg
     3361 agctgattga cgcgtcgcct tacgttaaga aatacacctc cggggtactg tcgtttgcgg
     3421 aagcggattt accctccggc cagcgggaaa agctgatggc aagctttgag cgggttctga
     3481 tgcccggact tgataaagac cagtacagca tcctgtgggt ggagcatcgt gacaaggggc
     3541 ggcttgagct gaattttctg atcccgaaca cggagctgct gaccggcaga cggctccagc
     3601 cgtattacga tcgcgccgac cgtccacgca tcgatgcctg gcagaccatc gtgaacggca
     3661 gactggggct gcacgacccg aacgcaccgg agaaccggcg ggcactggtc acaccgtctg
     3721 cactgcctaa agcgaagcag gaggctgcag aagcgattac acgcggtctg cttgctcttg
     3781 cctcatccgg ggagctgaaa acccgtcagg atgtcactga ggcgctggaa agcgcaggtt
     3841 ttgaggtggt ccgcaccaca aaaagcagta tcagcattgc cgacccggac ggggggcgaa
     3901 acatccgact gaagggagcc atttatgaac agtcttttaa cgctggcgaa ggacttagag
     3961 cagaaatcga aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc attcagcgag
     4021 cacgagaaat ctgtaaaagc ggaactgaac gaaagcgcga agagaatcag cgccgccatc
     4081 ctcgaccacg accggaaact gtcctcagcc atgagccagc gcacgaaagg catggtgcgc
     4141 atggtcagcc agacgtggct gaccatcgtc ctggtctccg cgctgctgat cgcctcgagc
     4201 gcgggcattc tgtggtggca ggggcagcag atactcgaca actacacgac catccgggag
     4261 cagaagagca cgcaggccat gctgtcagag aggaacagcg gcgtgcagct ctcgacctgc
     4321 ggcgagcaga gacgccgctg cgtgaaggtg aacccggaag cgggacggtt cggagaggat
     4381 tcgagctgga tgatactggc ggggaaatag cacatgacgg agctggaaaa acagttgctg
     4441 agcgcattag agcagctaca gcaggactac tcgaaaaggc tggacgagtg ggagagcgcc
     4501 ttcgcggaat ggcggacgat gtctggtctt atgcaacggg agaacgcagc gctgagcgag
     4561 cgcgtcacgg gcttgagcag gcaggtgcag agtttgagcg agcagctgcg ccggttgtcg
     4621 agcgactgaa tgccgtcgag ctgcaccggg agcaggagcg cgtggtg
//
