LOCUS       NZ_CP056135             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00916 plasmid pRHBSTW-00916_4,
            complete sequence.
ACCESSION   NZ_CP056135
VERSION     NZ_CP056135.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148743
            Assembly: GCF_013726395.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T3-00916
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 175.125454562702x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013726395.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:56:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,653
            CDSs (total)                      :: 4,539
            Genes (coding)                    :: 4,448
            CDSs (with protein)               :: 4,448
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 33 of 91
            Pseudo Genes (incomplete)         :: 56 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 18 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00916"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00916_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(41..268)
                     /locus_tag="HV326_RS23195"
                     /old_locus_tag="HV326_23205"
     CDS             complement(41..268)
                     /locus_tag="HV326_RS23195"
                     /old_locus_tag="HV326_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1476..1694
                     /locus_tag="HV326_RS23200"
                     /old_locus_tag="HV326_23210"
     CDS             1476..1694
                     /locus_tag="HV326_RS23200"
                     /old_locus_tag="HV326_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1985..2170)
                     /locus_tag="HV326_RS23205"
                     /old_locus_tag="HV326_23215"
     CDS             complement(1985..2170)
                     /locus_tag="HV326_RS23205"
                     /old_locus_tag="HV326_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1851..1938
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(684..1212)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 agaaaaaggc cacattagcg gcctttttcg gagaagatgc tcagtgcgga atttcggaga
       61 cttctttaac gtcggtttta ttgatctgct gtttgacacc attcgcgtct gtatagccaa
      121 gcaggcctga gtctgattct ttaggtttgc cgtcgctgac aatagtgcga ccgtcattgg
      181 tgtgtatggc atagcttgtg cgcgtgcagc cggtgagggc tgaaagcgca actgccgctg
      241 cgaacactga aacaaaaatc tttttcaaag ccagctcctt ttatccatat cagttttggt
      301 cactaaccat ctaactatag gtcatttttt atgtgaaaag aggcttgatg gtgggctgta
      361 tatcgagtta ggcgcgaggt gctatggtca aagtgtggtg tcaggcgggg aggaggctta
      421 tatgagatgt aatccggccg atacatatac caagatgatt taagtattcc tctttgtgag
      481 gttggatgtc taatttataa aggatcttct tgagatcctt tttttccgcg cgtaatctct
      541 tgccctgtaa acgaaaaaac caccctggca ggtggttttt cgaaggttag gtaatcctgg
      601 cagatcccct aaccgtggta acagtcttgt gcgagacatg tcaccaaatt tgtcctttca
      661 gtgtagcctc actaaggccg ccacttcaag aactcttgag acatctctcg cacatcctgt
      721 ttgccaatgg ccgttgccaa tggcgattag tcgtgtcttt ccgggttgga ctcaagttga
      781 tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccatc
      841 ctggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagaaa ccgcggccat
      901 aacagcggag tgacaccggt aaaccgaaag gcaggaatgc gggggagcac gagggagcca
      961 ccagggggaa acgcctggta tctttaagcc gcatcgggtt tcgccaccac tgatttgagc
     1021 gtcagattct gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttggcgcgg
     1081 ccttatgctt tcttcgttaa gtatcttcct ggcatccccc aggaaatttc tgatccatcc
     1141 gtaagcccgt cccgctcgcc gcagccgaac gaccgagcgg agcgagtcag tgagcgagga
     1201 agcggaatat attctgtatc acattttctc ctgacgcgtt ttctttcact ttctgcgcct
     1261 gtcttatgtg gcattaatgc tatgtgttac tgccatgcta catcttaagc cagtatacac
     1321 tccgctagtg ctccgtgact ggtccggcgc tgcgcccgga acccgcctgt accggttcag
     1381 cagccgttcc ggcctgactg caattttttt tttttcatcc ctgcccgcta ccctgtaaac
     1441 ctttcttctg cgttgccgtt aacagtaggt aagccatgtt tggtttttct tccggagccc
     1501 tgatgactct tagtgatgat gagcgacacc tgcttgtgtc cgttgtttcg gtctggctac
     1561 ggcgtgccgg aggggatgcc ggagccatga tgctggatgc ataccggcag attttgtctg
     1621 aaaccgaacc ggccgtaagg actgtcatgc tcgaatttct tgaatccgtt cgcattcatt
     1681 acatttcgtc ctgagcattt tctgtatttc tgtccatact taaacgaggc ggccgtcacg
     1741 gacggtggtc gtcgttccat gatgctgtta tggacgagtg agagtaatct aatctaactt
     1801 gattgagttc cgggcgtgcc gcccggtttt ttttgctgtt tcccggtgtc cgttccggga
     1861 caggatgtgc atttgtaata ccgcgcacgc gccggcatca caaataccct ggtcagggct
     1921 gcgccccgac accccgccat cgcctcacgg ccgcgcactg aattgcgcac ccgttcaccg
     1981 attttcacgc attttcctcg cctatctgtg cgttcagcat ggcgtaaagc gcttcggcct
     2041 gatcgtgcag gtgctcacag cagccggcag cctcatccag atccatctgc tcgagccgct
     2101 caaggaggag aagtgtctgt gcgcggataa atttggccat attgagggcg gcggattcct
     2161 gtctggtcac tggtgatttt ccccgtctgg tgatgatttt tgcgtgaaaa gagcatggcg
     2221 tcattctggt gcatagtcat gcatgccgtt aaaatttaac aggcgcgttt ctgacgggtt
     2281 ccggggtggt ttgttgtggt ttttgtcatg gtttcgtcag gaacgcgctg aacgcgtctc
     2341 aggcggtgcg ggcaacggat gttatggtaa attcttgggg gcttgattat aaaataccgc
     2401 acttcctctt atgtcttgtg ctttttagac aaccctgaaa gcttttagct ttggtgatgc
     2461 aatttgctgg gattttggcg gagaaccaca ggtaa
//
