LOCUS       NZ_CP056134             3875 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain RHBSTW-00916 plasmid pRHBSTW-00916_3,
            complete sequence.
ACCESSION   NZ_CP056134
VERSION     NZ_CP056134.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148743
            Assembly: GCF_013726395.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T3-00916
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 175.125454562702x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013726395.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:56:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,653
            CDSs (total)                      :: 4,539
            Genes (coding)                    :: 4,448
            CDSs (with protein)               :: 4,448
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 33 of 91
            Pseudo Genes (incomplete)         :: 56 of 91
            Pseudo Genes (internal stop)      :: 23 of 91
            Pseudo Genes (multiple problems)  :: 18 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056134.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00916"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:158836"
                     /plasmid="pRHBSTW-00916_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(156..581)
                     /locus_tag="HV326_RS23170"
                     /old_locus_tag="HV326_23180"
     CDS             complement(156..581)
                     /locus_tag="HV326_RS23170"
                     /old_locus_tag="HV326_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_045261372.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMGSKQQ"
                     /name="PH domain-containing protein"
     gene            1774..2097
                     /locus_tag="HV326_RS23175"
                     /old_locus_tag="HV326_23185"
     CDS             1774..2097
                     /locus_tag="HV326_RS23175"
                     /old_locus_tag="HV326_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2306..>2740
                     /locus_tag="HV326_RS23400"
                     /pseudo=""
     CDS             2306..>2740
                     /locus_tag="HV326_RS23400"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2775..3266
                     /locus_tag="HV326_RS23185"
                     /old_locus_tag="HV326_23195"
     CDS             2775..3266
                     /locus_tag="HV326_RS23185"
                     /old_locus_tag="HV326_23195"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012564908.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAVFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3273..3488
                     /locus_tag="HV326_RS23190"
                     /old_locus_tag="HV326_23200"
     CDS             3273..3488
                     /locus_tag="HV326_RS23190"
                     /old_locus_tag="HV326_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1489..1661
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="100.0"
                     /evalue="9.27e-76"
     Typing          complement(1026..1564)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.689"
                     /coverage="92.0"
                     /evalue="4.22e-159"
     Typing          2088..3580
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcgttatg tctgtcgcca ctttctcgcg tctggcaccg gaaacgcagc caaccccgtt
       61 tcagcggtgc gggcaacgga tgttatggta aattcagctg attggcaggt acaaaaaaag
      121 ccccgtaggg ctttttttgt aatgctacga ggaagctact gctgcttaga acccatttcc
      181 tcaatatgat tgttcacaat cgatctgaat tgcattggcg ccttgatgtt tggaattggc
      241 gtgttggttc cgccagtgcc tttaatcacg atagacccat agttaaagat tcgtcccagt
      301 atcccttgat gtatgccaag gctttcaact ttatcaatac ggagttccac cgtattgcgt
      361 cggatcaaac ccacttttgc gatgactctt tggttagtta atgcaagctc cgttgaccaa
      421 actctaagga gcacagggat tattataagt aaccctaagc cgtaaaaagg cagcaacaag
      481 atcccgataa ttactgggat gattgttgga atccaggagg tttgagcgcg agatattacg
      541 gattcatttc tcgtcaatga ttggtctacg tagcttccca taaggttata tccatttaga
      601 aaaaatagga tttttccctt tttaaggcga gtcagcaata gaaactttat gtctccgtgg
      661 taggtataca aaatttacgg agaacgtagc gaccgtcgga cagttcggtt ctttcctttc
      721 gtgccgcaac tggcttgatt ctgtggtggt ggtggtggcg gctgctatgc ctcatatact
      781 cctggcgttg caatccttta tcccatatct gtcgaatatc acagatgatc ttcttgagat
      841 cctgtccgtt gcgtcgtaat ctcttgctct gaaaacgaaa aaaccgccct gcaaggcggt
      901 ttttcgaagg ttctcagagc taccaactct ttgaaccgcg gtaactggct tggaggagcg
      961 cagtaaccaa atctgtcctt tcagtttagc cttaacaggc gcataacttc aagactaact
     1021 cctctaaatc cgttaccaat ggctaccgcc actggcgatt tttcgtgtct gttccgggtt
     1081 ggactcaaga tgatagttac cgggacaacg tggtagtcgg gctgaacggg gggttcttgc
     1141 acacagccca gcttggagcg aactgtctaa acggaacggg acgtggtgat ttaggtaaag
     1201 cttccaccac aacacggatg ccgcagggcg ggaacaggag agcgcaagag ggagccacca
     1261 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     1321 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt cgccgcggcc
     1381 ttctcgcttc tctgctaagt atcttcctgg catcctccag gaaatttccg ccccgtccgt
     1441 aagccattcc cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1501 cggaatatat tctgtatcac atattccact tacgctccgc tgctgcattt ttctcctgcc
     1561 acatgaagca cttcactgat atccgcatcc gtgccaacat agtaagccag tatacactcc
     1621 gctagcgcta cgtgactggt tcagggctgc gccccgaaac ccgccaaaag cctgacgcgc
     1681 ctgcggcttg tccaaccccg cgccgaccgg gaaaggcttt cccgcccgtc ccggcgttat
     1741 caggaggccg gtttgtcaga gaaacggagc aagatgctca ccatgtgggt gacggaggac
     1801 gagcaccggc ggctgctcga gcgctgtgac ggcaggcagc tggcggcgtg gatgcggcag
     1861 atctgcctgg acgaaaagcc ggcacgttcc ggaaagctcc cctcactctc gccggcgctg
     1921 ctgcgccagc ttgccggcat gggcaacaac ctgaaccaga tagcccgccg tgttaacgcc
     1981 ggcggcggca ccggccatga cagggtacag attgtggcgg cgctgatggc catcgatgcc
     2041 ggccttgagc ggctgcggca cgccgttctt gagaaaggaa cggacgatga tcgttaaatt
     2101 tcatccgcgc ggccgcggcg gtggcgccgg gccggtggac tatctgctgg gtaaagaccg
     2161 gcagcgtgaa ggcgccaccg tcctgcaggg gaagccggaa gaagtccggg agctgattga
     2221 cgcgtcgcct tacgttaaga aatacacctc cggcgttctg tcgtttgcgg aggccgatct
     2281 gcctcccggc cagcgggaaa aactgatggc cagcttcgag cgggttctga tgcccggact
     2341 cgataaagat cagtacagca ttctgtgggt ggagcacgcg gacaaggggc gtctggagct
     2401 gaactttctg atccccaata ccgagctgct gaccggcagg cggctccagc cttactacga
     2461 ccgcgccgac cggccgcgca tcgatgcctg gcagactatc gtgaacggca gactggggct
     2521 gcacgacccg aacgcgccgg aaaaccgccg ggcgctggtc acgccgtccg ggctgccgaa
     2581 aacgacgcag gaggccgcag aagccattac ccggggactg ctgacccttg cttcgtccgg
     2641 ggagctgaaa agccgtcagg atgtcactga cactcttgaa ggtgcaggtt ttgaggtggt
     2701 gcgcaccacg aaaagcagta tcagcattgc cgacccggac ggggggcgaa acatccgact
     2761 gaagggagcc atctatgagc agtcttttaa cgctggcgaa ggacttagag cagcaatcga
     2821 aagcgcagca gcagagtacc ggcgagatgc tgaaagccgt attcagcgag cacgagcggt
     2881 ctgtcagagc ggaactgagc gcaagcgcga ggagaatcag cgacgccatc ctcgcccacg
     2941 agcagagtat gagcgaggcg atggagaaaa accggcggag cgtactgcgg actgccgggc
     3001 ggacatggct caccatcctg atggtgtcag cgctgctgat cggcacgagc gggagcattc
     3061 tgtggtggca ggggcagcag ataaccgaca attacacgca tcttcgtcag caggaagata
     3121 ccctggcgaa aatgaccgcc cggacgtggg gagtgcgcta tcaggagagc agcgacggcc
     3181 ggaggtttct tatcctgccg ccggggatgc aaaccgaagc catcccctac gacgggacga
     3241 catggatccg cctgaaacag gagtagcgga aaatgacaga gctggaaaag cagttgctga
     3301 gcgcattaga gcagctacag caggactact cgcaaaggct ggacgaatgg gagagcgcct
     3361 tcgcggaatg gcggagcatg tgtgggctta tgcaacggga gaacgcggtg ctgaacgagc
     3421 gcgtgtcgga cttgagcacg caggtgctga gtttaagcga gcagctgcgc cgcttgtccg
     3481 ggaactgaat gccaccgagt tccggcagag ggagaatgag tatcagaaaa cactggagct
     3541 ggagcgccag ccgcagaaaa cctaccgcgg gccgacactt taaaccatcc aggcagctga
     3601 caacccgcac gaagaggggc tcttatttct cccgctaaat aggttcgtga tcaaaaaggt
     3661 cacgacttat tcaggagcgg gaaatgaagc tgttaaatct tattgaatca ttgcttaact
     3721 gcctgtatct ggcggcggag ctgtgggact ggctggagaa gcatttgtcg gtttttaagg
     3781 cattggcgcg gattttagtg tgactttaac atccatgcat ctggctgcat gactatgcac
     3841 cagtgtaata ttcagcggct gcgtttctgg tgttt
//
