LOCUS       NZ_CP056040             1549 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli strain EC31 plasmid pEC31-4, complete sequence.
ACCESSION   NZ_CP056040
VERSION     NZ_CP056040.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15235523
            Assembly: GCF_018141125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Liu,F., Jiang,S. and Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2020) College of Animal Science and Technology,
            Shandong Agricultural University, 61 Daizhong Street, Taian 271000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. JANUARY-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology :: Oxford Nanopore PromethION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 02:49:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,288
            CDSs (total)                      :: 5,172
            Genes (coding)                    :: 4,986
            CDSs (with protein)               :: 4,986
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 58 of 186
            Pseudo Genes (incomplete)         :: 133 of 186
            Pseudo Genes (internal stop)      :: 31 of 186
            Pseudo Genes (multiple problems)  :: 35 of 186
            Pseudo Genes (short protein)      :: 4 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP056040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC31"
                     /host="meat duck"
                     /db_xref="taxon:562"
                     /plasmid="pEC31-4"
                     /geo_loc_name="China: Taian"
                     /collection_date="2017-10-26"
     gene            1..477
                     /locus_tag="HUW39_RS26190"
                     /old_locus_tag="HUW39_26190"
     CDS             1..477
                     /locus_tag="HUW39_RS26190"
                     /old_locus_tag="HUW39_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1069..1167
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.0"
                     /coverage="100.0"
                     /evalue="6.26e-40"
     Typing          complement(1363..1471)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(709..969)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.366"
                     /coverage="100.0"
                     /evalue="3.98e-101"
ORIGIN
        1 atgtttattg attcagaaaa acgactgaaa caactttcag atgaggcaaa gaaaaacacc
       61 gaggatctcg aagaagcaaa gaaaaattca aggtttacac aggtatcccc aaaaggttgg
      121 gaacgcgttc gagagctgct gaaggatagc caaggcatat cagcactgaa gctgtactca
      181 tttttagcgg agcatatcga tcctacgtgt ggcgctgtcg ttgcggatca gcaattccta
      241 gctgaaaaac ttggagttag cagaagcaca attattcgtt ggctcaatta cttagaatca
      301 aaaaatgcat tagttagaat ccccgttgct ggtaaggttt gtgcgtatgc cctcgatcca
      361 catgaagtct ggaagggata caacactacg aaaaaccatg cagcgtttgt cactaaaaca
      421 ctggtcaaca aagacggtga tattcagcgc cgaatcatgg ccatgttttc aaattgagct
      481 agcggcaggc ggacaatcag gggctacgtg ttaacgttct gaccatgatt gtctatcctg
      541 cattgctctt ttgccgcctc aaaatccttt gcgtgttttt gctccccgtt ctccagaaaa
      601 aaccgagccg ccacggttcc ggcagcgcct tgagcgctac cggaaaccgt ctggggagag
      661 ccccagaccc ccatcgctgc aacaccgacg gtctacgaca acctaccaac cgcatggctg
      721 cctgttccgt gcaaatgggc tttgcagggg gctggcgcgg ttcctggcga cctgaaagcc
      781 cctggttagg ggcatcggcg cgcctgacct cctgagagcc tctgtaagcg tttttcgcgc
      841 cttccatgcc ctggcgggca tctaaaaccc gtttcgtgcg tctgagagct tgtgagcgcg
      901 tttgaggggc tatctggcga ctcatccccg aaagaacaac aacgggatta ccgacaacat
      961 gcgagaggtg atcccgctcg ccgcaagtcg atgaccgagc gtagcgagcg aatcgacgag
     1021 gaagcggaag agaaccggaa gccacattga gcacttacgc actgatgcgg ggtgtcgggg
     1081 tgaagccctg acaaggtggt aatcgtatcg gcgtgcgtgc gcggttatac gattacacat
     1141 cctgtcccga tttctgaggc gttttaacgg tgaacggacg gcaaaaacac gatggagctg
     1201 ttgaacgtga aaaccacgaa atctggcaaa aatcacggct ggcgaggctt gcagaggagt
     1261 aagccagtat acactccgct atcgctacgt gactgggtca gggcttcgcc ccgacacccc
     1321 caaagggtgt tgcgttgcac gcaacaccct tgcctagaat agatctttta cgccgatttg
     1381 taagttgttg tttttttagt gtgtttttta tttacctgtc gcatccaggg tgacacaacg
     1441 ttgtcgcatc ctggatgcga caacacgcaa caaggtgttg cacggatgtg tcatgatggc
     1501 ttagatttga accaacgtta gtgcatggga ttttcagagg gaaaaaatc
//
