LOCUS       NZ_CP055887             3875 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Enterobacter sp. RHBSTW-00975 plasmid pRHBSTW-00975_5, complete
            sequence.
ACCESSION   NZ_CP055887
VERSION     NZ_CP055887.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148757
            Assembly: GCF_013615235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. RHBSTW-00975
  ORGANISM  Enterobacter sp. RHBSTW-00975
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00975
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 193.800753795745x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013615235.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:40:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,884
            CDSs (total)                      :: 4,765
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 26 of 89
            Pseudo Genes (incomplete)         :: 64 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 20 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055887.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterobacter sp. RHBSTW-00975"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00975"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742673"
                     /plasmid="pRHBSTW-00975_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3803..3875,1..353)
                     /locus_tag="HV340_RS24300"
                     /old_locus_tag="HV340_24310"
     CDS             join(3803..3875,1..353)
                     /locus_tag="HV340_RS24300"
                     /old_locus_tag="HV340_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_045261372.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMGSKQQ"
                     /name="PH domain-containing protein"
     gene            complement(896..1111)
                     /locus_tag="HV340_RS24305"
                     /old_locus_tag="HV340_24315"
     CDS             complement(896..1111)
                     /locus_tag="HV340_RS24305"
                     /old_locus_tag="HV340_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1118..1609)
                     /locus_tag="HV340_RS24310"
                     /old_locus_tag="HV340_24320"
     CDS             complement(1118..1609)
                     /locus_tag="HV340_RS24310"
                     /old_locus_tag="HV340_24320"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_044715458.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1644..2348)
                     /locus_tag="HV340_RS24650"
                     /old_locus_tag="HV340_24325"
                     /pseudo=""
     CDS             complement(<1644..2348)
                     /locus_tag="HV340_RS24650"
                     /old_locus_tag="HV340_24325"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2287..2610)
                     /locus_tag="HV340_RS24320"
                     /old_locus_tag="HV340_24330"
     CDS             complement(2287..2610)
                     /locus_tag="HV340_RS24320"
                     /old_locus_tag="HV340_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2724..2896)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="100.0"
                     /evalue="9.27e-76"
     Typing          2821..3356
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.791"
                     /coverage="91.0"
                     /evalue="4.22e-159"
     Typing          complement(805..2297)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caacaatcat cccagtaatt atcgggatct tgttgctgcc tttttacggc ttagggttac
       61 ttataataat ccctgtgctc cttagagttt ggtcaacgga gcttgcatta actaaccaaa
      121 gagtcatcgc aaaagtgggt ttgatccgac gcaatacggt ggaactccgt attgataaag
      181 ttgaaagcct tggcatacat caagggatac tgggacgaat ctttaactat gggtctatcg
      241 tgattaaagg cactggcgga accaacacgc caattccaaa catcaaggcg ccaatgcaat
      301 tcagatcgat tgtgaacaat catattgagg aaatgggttc taagcagcag tagcttcctc
      361 gtagcattac aaaaaaagcc ctacggggct ttttttgtac ctgccaatca gctgaattta
      421 ccataacatc cgttgcccgc accgctgaaa cggggttggc tgcgtttccg gtgccagacg
      481 cgagaaagtg gcgacagaca taacgaaaaa acaccagaaa cgcagccgct gaatattaca
      541 ctggtgcata gtcatgcagc cagatgcatg gatgttaaag tcacactaaa atccgcgcca
      601 atgccttaaa aaccgacaaa tgcttctcca gccagtccca cagctccgcc gccagataca
      661 ggcagttaag caatgattca ataagattta acagcttcat ttcccgctcc tgaataagtc
      721 gtgacctttt tgatcacgaa cctatttagc gggagaaata agagcccctc ttcgtgcggg
      781 ttgtcagctg cctggatggt ttaaagtgtc ggcccgcggt aggttttctg cggctggcgc
      841 tccagctcca gtgttttctg atactcattc tccctctgcc ggaactcggt ggcattcagt
      901 tcccggacaa gcggcgcagc tgctcgctta aactcagcac ctgcgtgctc aagtccgaca
      961 cgcgctcgtt cagcaccgcg ttctcccgtt gcataagccc acacatgctc cgccattccg
     1021 cgaaggcgct ctcccattcg tccagccttt gcgagtagtc ctgctgtagc tgctctaatg
     1081 cgctcagcaa ctgcttttcc agctctgtca ttttccgcta ctcctgtttc aggcggatcc
     1141 atgtcgtccc gtcgtagggg atggcttcgg tttgcatccc cggcggcagg ataagaaacc
     1201 tccggccgtc gctgctctcc tgatagcgca ctccccacgt ccgggcggtc attttcgcca
     1261 gggtatcttc ctgctgacga agatgcgtgt aattgtcggt tatctgctgc ccctgccacc
     1321 acagaatgct cccgctcgtg ccgatcagca gcgctgacac catcaggatg gtgagccatg
     1381 tccgcccggc agtccgcagt acgctccgcc ggtttttctc catcgcctcg ctcatactct
     1441 gctcgtgggc gaggatggcg tcgctgattc tcctcgcgct tgcgctcagt tccgctctga
     1501 cagaccgctc gtgctcgctg aatgcggctt tcagcatctc gccggtactc tgctgctgcg
     1561 ctttcgattg ctgctctaag tccttcgcca gcgttaaaag actgctcata gatggctccc
     1621 ttcagtcgga tgtttcgccc cccgtccggg tcggcaatgc tgatactgct tttcgtggtg
     1681 cgcaccacct caaaacctgc accttcaaga gtgtcagtga catcctgacg gcttttcagc
     1741 tccccggacg aagcaagggt cagcagtccc cgggtaatgg cttctgcggc ctcctgcttc
     1801 gttttcggca gcccggacgg cgtgaccagc gcccggcggt tttccggcgc gttcgggtcg
     1861 tgcagcccca gtctgccgtt cacgatagtc tgccaggcat cgatgcgcgg ccggtcggcg
     1921 cggtcgtagt aaggctggag ccgcctgccg gtcagcagct cggtattggg gatcagaaag
     1981 ttcagctcca gacgcccctt gtccgcgtgc tccacccaca gaatgctgta ctgatcttta
     2041 tcgagtccgg gcatcagaac ccgctcgaag ctggccatca gtttttcccg ctggccggga
     2101 ggcagatcgg cctccgcaaa cgacagaacg ccggaggtgt atttcttaac gtaaggcgac
     2161 gcgtcaatca gctcccggac ttcttccggc ttcccctgca ggacggtggc gccttcacgc
     2221 tgccggtctt tacccagcag atagtccacc ggcccggcgc caccgccgcg gccgcgcgga
     2281 tgaaatttaa cgatcatcgt ccgttccttt ctcaagaacg gcgtgccgca gccgctcaag
     2341 gccggcatcg atggccatca gcgccgccac aatctgtacc ctgtcatggc cggtgccgcc
     2401 gccggcgtta acacggcggg ctatctggtt caggttattg cccatgccgg caagctggcg
     2461 cagcagcgcc ggcgagagtg aggggagctt tccggaacgt gccggctttt cgtccaggca
     2521 gatctgccgc atccacgccg ccagctgcct gccgtcacag cgctcgagca gccgccggtg
     2581 ctcgtcctcc gtcacccaca tggtgagcat cttgctccgt ttctctgaca aaccggcctc
     2641 ctgataacgc cgggacgggc gggaaagcct ttcccggtcg gcgcggggtt ggacaagccg
     2701 caggcgcgtc aggcttttgg cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct
     2761 agcggagtgt atactggctt actatgttgg cacggatgcg gatatcagtg aagtgcttca
     2821 tgtggcagga gaaaaatgca gcagcggagc gtaagtggaa tatgtgatac agaatatatt
     2881 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcgggaatgg
     2941 cttacggacg gggcggaaat ttcctggagg atgccaggaa gatacttagc agagaagcga
     3001 gaaggccgcg gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
     3061 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3121 ccctggtggc tccctcttgc gctctcctgt tcccgccctg cggcatccgt gttgtggtgg
     3181 aagctttacc taaatcacca cgtcccgttc cgtttagaca gttcgctcca agctgggctg
     3241 tgtgcaagaa ccccccgttc agcccgacta ccacgttgtc ccggtaacta tcatcttgag
     3301 tccaacccgg aacagacacg aaaaatcgcc agtggcggta gccattggta acggatttag
     3361 aggagttagt cttgaagtta tgcgcctgtt aaggctaaac tgaaaggaca gatttggtta
     3421 ctgcgctcct ccaagccagt taccgcggtt caaagagttg gtagctctga gaaccttcga
     3481 aaaaccgcct tgcagggcgg ttttttcgtt ttcagagcaa gagattacga cgcaacggac
     3541 aggatctcaa gaagatcatc tgtgatattc gacagatatg ggataaagga ttgcaacgcc
     3601 aggagtatat gaggcatagc agccgccacc accaccacca cagaatcaag ccagttgcgg
     3661 cacgaaagga aagaaccgaa ctgtccgacg gtcgctacgt tctccgtaaa ttttgtatac
     3721 ctaccacgga gacataaagt ttctattgct gactcgcctt aaaaagggaa aaatcctatt
     3781 ttttctaaat ggatataacc ttatgggaag ctacgtagac caatcattga cgagaaatga
     3841 atccgtaata tctcgcgctc aaacctcctg gattc
//
