LOCUS       NZ_CP055886             4665 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Enterobacter sp. RHBSTW-00975 plasmid pRHBSTW-00975_4, complete
            sequence.
ACCESSION   NZ_CP055886
VERSION     NZ_CP055886.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148757
            Assembly: GCF_013615235.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter sp. RHBSTW-00975
  ORGANISM  Enterobacter sp. RHBSTW-00975
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T3-00975
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 193.800753795745x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013615235.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:40:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,884
            CDSs (total)                      :: 4,765
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 26 of 89
            Pseudo Genes (incomplete)         :: 64 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 20 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055886.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Enterobacter sp. RHBSTW-00975"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00975"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:2742673"
                     /plasmid="pRHBSTW-00975_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4306..4665,1..1197)
                     /locus_tag="HV340_RS24575"
                     /old_locus_tag="HV340_24285"
     CDS             join(4306..4665,1..1197)
                     /locus_tag="HV340_RS24575"
                     /old_locus_tag="HV340_24285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            2255..2578
                     /locus_tag="HV340_RS24280"
                     /old_locus_tag="HV340_24290"
     CDS             2255..2578
                     /locus_tag="HV340_RS24280"
                     /old_locus_tag="HV340_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2787..>3221
                     /locus_tag="HV340_RS24580"
                     /pseudo=""
     CDS             2787..>3221
                     /locus_tag="HV340_RS24580"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3256..3741
                     /locus_tag="HV340_RS24290"
                     /old_locus_tag="HV340_24300"
     CDS             3256..3741
                     /locus_tag="HV340_RS24290"
                     /old_locus_tag="HV340_24300"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3745..3960
                     /locus_tag="HV340_RS24295"
                     /old_locus_tag="HV340_24305"
     CDS             3745..3960
                     /locus_tag="HV340_RS24295"
                     /old_locus_tag="HV340_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_012565230.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1969..2142
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(1493..2045)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.741"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2256..2572
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.81e-61"
     Typing          2569..4064
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttagttggta cccgcttgaa ttcaagtgcc agcaaattgg cgattgagga aaagaaacca
       61 ttcatatctt caccgttaaa atcagttact gggaatttga ttattttgtt gtcacatcct
      121 atttatgata agggtggcaa ttacataggg tatgtcggtg ggtctatata tctaaaaaaa
      181 cataatttat ttggcgatgt tttgagcagg cattttttca agaacaacac tgaaattagc
      241 attgttagtg atgatgggaa tgtgatcttt aataacgata aaagtgctgt tgatcggcct
      301 atggttatgt ctgatgattt gaaatcaaag ttaactgcgt ctgatagagg tgagggggca
      361 tttttttctt cggggcatag atatcttctt ggttatgcta gtatgaaaaa tactgcttgg
      421 aatgtgttgg tttatagtcg tgctgacagt gtgactacta ttcttattgc ttatgctaaa
      481 agtgtacttg ttctgctaat tataataatt attgtgtttt cacttatttc atatttcatt
      541 gcctccagta tttcacttcc tttagaaaag cttgctatat ctaccaatgc taaagatatc
      601 ggagcttcgc tagtttatat taaagctatc aatgcttggt atgccgaagc tgataggctt
      661 aaaaaggcgc tttttacgta tattaaggta atgatggaaa gagttgattt tctaaatgag
      721 gtggcgctta aagatcccct taccggcgtg aacaatagat tggggttttt aagtaaaata
      781 cagaatcata agcgggattc agggtattca gttattgtaa ttgatgttga tttttttaaa
      841 gagataaatg acagtttcgg gcatggggtc ggagatgagg tgcttgtttc tttggctcat
      901 attattaatt cctgttgccg tagtgaagat gttgtttgtc ggtttggtgg cgaagagttt
      961 gttgttttcc tgccgaatac atctgtggta acagctaagt gtgttgcgga gcgtattagg
     1021 agtgttattg aaagtactgt cttccctaat aacttgcggg tcaccatatc tgctggtgtg
     1081 gcaacacaag atgatcctct tggtaggatc gattttttgc tgaaaaatgc tgacgatgct
     1141 ctttatcaag ccaaaaggga ggggcgtaat aaagtgattg tatatagcgc tatttgagtt
     1201 ttttgatgat gctttacgcg ctagtgtgtc gttttttttg tggcgaagag tgggcaagaa
     1261 gcggatcctc ttgttatccc ttcgatgtcg tcgtaatctc ataccctgta aacaaaaaaa
     1321 ccaccgctac caacggtggt tcgtttgccg gaattcagag ctaccaactc tttcttccga
     1381 aggtaactgg ctttcacgga gcgcaggtac caaaactgtc cttccagtgt agccgcagtc
     1441 aggccaccac ttcaagaact ctgtagaaca ctctactact ccgctccgtg aatcctctta
     1501 ctaatggcta ccgccactgg cgatttttcg tgtctgttcc gggttggact caagatgata
     1561 gttaccggga caacgtggta gtcgggctga acggggggtt cgtgcataca gtccagcttg
     1621 gagcgaactg ccttcccgga accgagtgtc aggcgtggaa tgagataaac gcggccataa
     1681 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccacc
     1741 agggggaaac gcctggtatc tttaagtcct gtcgggtttc gccaccactg atttgagcgt
     1801 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt cgccgcggcc
     1861 ttctcgcttc cctgttacgt tccttcctgg catcttccag gaaatttccg ccccacctgt
     1921 aagccggcac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1981 cggaatatat cctgtatcac atattctgct gacgcgccgg tgccgccttt tttctcctgc
     2041 cacatgaagc acttcactga caccctcatc cgtgccaaca tattaagcca gtatacactc
     2101 cgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgccaaaa gcctgacgcg
     2161 cctgcggctt gtccaacccc gtgccgaccg ggaaaggttt tcccgcccgt cccggcgtta
     2221 tcaggagacc ggtttgtcag agaaacggaa caagatgctc accatgtggg tgacggagga
     2281 cgagcaccgg cggctgcttg agcgctgtga cggcaggcag ctggcggcgt ggatgcggca
     2341 gacctgcctg gacgaaaagc cggcacgttc cggaaagctc ccctcaatct cgccggcgct
     2401 gctgcgccag cttgccggca tgggcaataa cctgaaccag atagcccgcc gtgttaacgc
     2461 cggcggcggt accggccatg acagggtgca gattgttgcg gcgctgatgg ccatcgatgc
     2521 cggacttgag cggctgcggc acgccgttct tgagaaagga acggacgatg atcgttaaat
     2581 ttcatccacg cggtcgcggc ggtggcgccg ggccggtgga ctatctgctg ggtaaagacc
     2641 ggcagcgtga aggcgccacc gtcctgcagg ggaagccgga agaagtccgg gagctgattg
     2701 acgcgtcgcc ttacgttaag aaatacacct ccggggtact gtcgtttgcg gaagcggatt
     2761 taccttccgg ccagcgggaa aagctgatgg caagctttga gcgggttctg atgcccggac
     2821 ttgataaaga ccagtacagc atcctgtggg tggagcatcg tgacaagggg cggcttgagc
     2881 tgaattttct gatcccgaac acggagctgc tgaccggcag acggctccag ccgtattacg
     2941 atcgcgccga ccgtccacgc atcgatgcct ggcagaccat cgtgaacggc agactggggc
     3001 tgcacgaccc gaacgcaccg gagaaccggc gggcactggt cacaccgtct gcactgccta
     3061 aagcgaagca ggaggctgca gaagcgatta cacgcggtct gcttgctctt gcctcatccg
     3121 gggagctgaa aacccgtcag gatgtcactg aggcgctgga aagcgcaggt tttgaggtgg
     3181 tccgcaccac aaaaagcagt atcagcattg ccgacccgga cggggggcga aacatccgac
     3241 tgaagggagc catttatgaa cagtctttta acgctggcga aggacttaga gcagaaatcg
     3301 aaagcgcagc agcagaatac cggcgagatg ctgaaagccg cattcagcga gcacgagaaa
     3361 tctgtaaaag cggaactgaa cgaaagcgcg aagagaatca gcgccgccat cctcgaccac
     3421 gaccggaaac tgtcctcagc catgagccag cgcacgaaag gcatggtgcg catggtcagc
     3481 cagacgtggc tgaccatcgt cctggtctcc gcgctgctga tcgcctcgag cgcgggcatt
     3541 ctgtggtggc aggggcagca gatactcgac aactacacga ccatccggga gcagaagagc
     3601 acgcaggcca tgctgtcaga gaggaacagc ggcgtgcagc tctcgacctg cggcgagcag
     3661 agacgccgct gcgtgaaggt gaacccggaa gcgggacggt tcggagagga ttcgagctgg
     3721 atgatactgg cggggaaata gcacatgacg gagctggaaa aacagttgct gagcgcatta
     3781 gagcagctac agcaggacta ctcgaaaagg ctggacgagt gggagagcgc cttcgcggaa
     3841 tggcggacga tgtctggtct tatgctacgg gagaacgcag cgctgagcga gcgcgtcacg
     3901 ggcttgagca ggcaggtgca gagtttgagc gagcagctgc gccggttgtc gagcgactga
     3961 atgccgtcga gctgcaccgg gagcaggagc gcgtggtgca gcatcagaaa gcgctggagc
     4021 tggagcgatc tcagcgacag cggacgtata acagcccttc actgtgataa cctgataaaa
     4081 cggggctttc ggcggacatc tgcgctgaaa gccggagcca ttcacccgac tgcatagcca
     4141 tgcagccggg tgaaatttat ctggcgcaga atgcgcggtt tagcggagag tttgctgccg
     4201 gttttaccgg gctgacgccg catttgttcc gaacccgtcc ggggcggtgc tgataaggga
     4261 tgttatggta aatgcggcat tttttattat atggagtgta tttttatgag aattaaaaag
     4321 cctcaccttc gtccgatagt cataaccttg tccgttggtg ggattttgtt aacttctatt
     4381 tttctgattg ccgttattat ggtttttcag cgcgaaaata tagaaaaaga tttgctttat
     4441 gctaattcat cgtacgctat gaaaatgtca gatgtaatga gtggttatat tgaaatggct
     4501 caaggtgaat tagcatatgg agcaaaaaaa ataaaatcaa ctaatgatat tgactcactt
     4561 aaagatgagg cggataggtt aagattgcag tctggtatgt ttaattcagt tcttgttgtt
     4621 agtaatgatg ctgttgttct ggcgacttcg ccagaatctc ttgat
//
