LOCUS       NZ_CP055679             6647 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Escherichia fergusonii strain RHB28-C13 plasmid pRHB28-C13_5,
            complete sequence.
ACCESSION   NZ_CP055679
VERSION     NZ_CP055679.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148269
            Assembly: GCF_013596835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T2-C13
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 159.376270081111x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013596835.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 05:20:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,940
            Genes (coding)                    :: 4,839
            CDSs (with protein)               :: 4,839
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 31 of 101
            Pseudo Genes (incomplete)         :: 64 of 101
            Pseudo Genes (internal stop)      :: 18 of 101
            Pseudo Genes (multiple problems)  :: 13 of 101
            Pseudo Genes (short protein)      :: 2 of 101
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055679.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB28-C13"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB28-C13_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6170..6647,1..1091))
                     /gene="cea"
                     /locus_tag="HVY52_RS25095"
                     /old_locus_tag="HVY52_25080"
     CDS             complement(join(6170..6647,1..1091))
                     /gene="cea"
                     /locus_tag="HVY52_RS25095"
                     /old_locus_tag="HVY52_25080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1351..1788
                     /locus_tag="HVY52_RS25100"
                     /old_locus_tag="HVY52_25085"
     CDS             1351..1788
                     /locus_tag="HVY52_RS25100"
                     /old_locus_tag="HVY52_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1869..2288
                     /locus_tag="HVY52_RS25105"
                     /old_locus_tag="HVY52_25090"
     CDS             1869..2288
                     /locus_tag="HVY52_RS25105"
                     /old_locus_tag="HVY52_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2606..2839)
                     /locus_tag="HVY52_RS25110"
                     /old_locus_tag="HVY52_25095"
     CDS             complement(2606..2839)
                     /locus_tag="HVY52_RS25110"
                     /old_locus_tag="HVY52_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2846..3364)
                     /locus_tag="HVY52_RS25115"
                     /old_locus_tag="HVY52_25100"
     CDS             complement(2846..3364)
                     /locus_tag="HVY52_RS25115"
                     /old_locus_tag="HVY52_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3402..4100)
                     /locus_tag="HVY52_RS25590"
                     /old_locus_tag="HVY52_25105"
                     /pseudo=""
     CDS             complement(<3402..4100)
                     /locus_tag="HVY52_RS25590"
                     /old_locus_tag="HVY52_25105"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4042..4365)
                     /locus_tag="HVY52_RS25125"
                     /old_locus_tag="HVY52_25110"
     CDS             complement(4042..4365)
                     /locus_tag="HVY52_RS25125"
                     /old_locus_tag="HVY52_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4429..4620
                     /locus_tag="HVY52_RS25130"
                     /old_locus_tag="HVY52_25115"
     CDS             4429..4620
                     /locus_tag="HVY52_RS25130"
                     /old_locus_tag="HVY52_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5290..5448
                     /locus_tag="HVY52_RS25595"
                     /old_locus_tag="HVY52_25120"
     CDS             5290..5448
                     /locus_tag="HVY52_RS25595"
                     /old_locus_tag="HVY52_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5647..5784)
                     /locus_tag="HVY52_RS25135"
                     /old_locus_tag="HVY52_25125"
     CDS             complement(5647..5784)
                     /locus_tag="HVY52_RS25135"
                     /old_locus_tag="HVY52_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            5946..6173
                     /locus_tag="HVY52_RS25140"
                     /old_locus_tag="HVY52_25130"
     CDS             5946..6173
                     /locus_tag="HVY52_RS25140"
                     /old_locus_tag="HVY52_25130"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4706..4793)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4804..5362
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2503..4052)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccatattttt cagtcagcgt ttgataaaag ctaactgttg catcaacagc atccttaatc
       61 tgtgaattaa ggagattatt ctgtgctttt ttcaaatttt cttcagcttc atgaacacga
      121 gcgataccgg cattacgatt attactgacc tgagaaatag ccttctggat ctgagttata
      181 tcagcattta tccggttaat acgtgtttct gatgctgtta cctgtttttg tttttcttct
      241 ctaatcttac cggccccaac ccgtcgtctg gttgcttcaa aaaaaggacg gttctgaagc
      301 ggatcattgg ctcttggtga tagttttttg accagctcat ccagttcttt atatttagcg
      361 gatgcctgag ccagttcatt tcgttttcca gcgagcgttt tcatttctgc atcacgggca
      421 tggatactgg agcttaaacg agaattgaga gtcttaatct ctccatccat tttcaccact
      481 tcagattgtg cagcagaaag ttttttttgg gcgatctcaa cagctttagc ttcttcactc
      541 aatgcagcca gtcgtttctc ttcagcttca gccagtttca actggcgttc tgtttcagcc
      601 ttctcccgtt caatctcttt acgtcgttgt tctgcttcct gaaaagcctt ttctgctgct
      661 tccgcttctt tacgggcttt ttcttctgct ttcgcaaggc gcaaacgctc tgcttccgcc
      721 tgcatagctg cattattagc atgagcaagc tctgttgctg aaggcgtacg tgaggcattg
      781 tgacgaagag cctcattcac gatatccttc aggcgctgag tcagcgcatc cctgtttgcc
      841 tttgctttcg cctgtgcttc cgctgcagct tttgcccggg cagcctgctc tgcctgtgtt
      901 ttctttaatt gagcagtaga ccatttagca gttgcatgaa tagctgcaga actttcactt
      961 ttactgcctc cttttccacc tccgccgcca gagccactcc cgtcaggagt accattcaaa
     1021 agagtaataa ttacctgtcc cttatcatca taaggaacac catctttata gtacgctacc
     1081 gcggtttcca ttataaaatc ctctttgact tttaaaacaa taagttaaaa ataaatactg
     1141 tacatataac cactggtttt atatacagca taaaagctac gccgctgcat tttccctgtc
     1201 aagactgtgg acttccattt ttgtgaaaac gatcaaaaaa acagtctttc acaccacgcg
     1261 ctattctcgc ccgatgccac aaaaaccagc acaaacatta ccgttctcag acctcattat
     1321 gttttactga aactatgaga tgagacatct atgggacact gtcactttat ggcatggcac
     1381 acactccggg acgcactaaa aatgacaggc agatcgcgtt cacagtttta ccgtgatatg
     1441 cgcgcaggcc ttgtcagtta ccgtaccggc agggacggac gacgggagtt tgaaaccagt
     1501 gaactgatcc gggcatacgg cgaattaaag cagaatgaga caccagaaag gcacagtgag
     1561 ggacatgcag aaaatccaca tgatcagcag acagaacgca ttctccggga gctgaatgag
     1621 ctgaaacaat gcctgacgct gatgcttgag gataaacagg cacaggatat ggatcgcaga
     1681 cgccaggaag cagaacggga acagctacaa aatgagatag cccagctcag gcaggcactg
     1741 gaactggaaa agaaacgggg attctggtcc aggttgttcg gtcgctgaac gctgtcagag
     1801 actgatgata aaatagtctt cggataataa ctcaccgaga ataaatactt taaggtaggg
     1861 agacactcat gagacgtacc ggaaacaaac tttgtcttat cgccatgata acagcaacag
     1921 tagctctcac agcctgtacc ccaaagggca gcgtggaaca acatacccgg cattacgtat
     1981 atgcttctga tgacggtttt gatcccaact tttccaccca aaaagccgac acaacacgaa
     2041 tgatggtgcc tttttttcgg cagttctggg atatgggagc taaagataaa gcgacaggaa
     2101 aatcacggag tgatgtgcaa caacgcattc agcagtttca cagccaggaa tttttaaact
     2161 cactccgggg cacaactcaa tttgcgggta ctgattaccg cagcaaagac cttaccccga
     2221 aaaaatccag gctgctggct gacacgattt ctgcggttta tctcgatggc tacgagggca
     2281 gacagtaagt ggatttacca taatccctta attgtacgca ccgctaaaac gcgttcagcg
     2341 cgatcacggc agcagacagg taaaaatggc aacaaaccac ccgaaaaact gccgcgatcg
     2401 cgcctgataa attttaaccg tatgaatacc tatgcaacca gagggtacag gccacattac
     2461 ccccacttaa tccactgaag ctgccatttt tcatggtttc accatcccag cgaagggcca
     2521 tccagcgtgc gttcctgtat ttccggctga cgctcccgtt ccagggataa cacatgttcg
     2581 cgctcctgta tcagccgttc ctctcttatc tccagttctc gctgtataac tggctcaagc
     2641 gttctgtctg ctcgctcaag tgctgcacct gctgactcaa ctgcatgacc cgctcgttca
     2701 gcatcgcgtt gtcccgttgc gtaagcgaaa acatcttctg caattccacg aaggcgctct
     2761 cccattcgct cagccgctgc atatagtcct gttgcagctg ctctaaggcg ttcagcaaat
     2821 gtgtttccag ctctgtcact ctgtgtcact ccttcagatg tacccactct ttcccctgaa
     2881 agggaatcac ctccgctgat ttcccgtacg gaaggacaag gaatttcctg ttcccgtcct
     2941 gcacaaactc cacgccccat gtcttcgcgt tcagtttctg caatgtctct tcctgcttcc
     3001 tgatttcttc caggttcgcc tgtatcctcc ctccaagata ccagagcgtc ccgccactcg
     3061 cggtaaacag gagaaagact atccccagta acatcatgcc cgtattccct gccagcttta
     3121 acacgtccct cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt ttttccagcg
     3181 attcctctgt cgaggctgtg aacagggcta tagcgtctct gattttcgtc tcgtttgatg
     3241 tcacagcctc gcttacagat tcgccgagcc tcctgaactc gttgttcagc attttctctg
     3301 tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgc aaaagattgc
     3361 tcataaaatg ctcctttcag cctgatattc ttcccgccgt tcggatctgc aatgctgata
     3421 ctgcttcgcg tcaccctgac cacttccagc cccgcctcag tgagcgcctg aatcacatcc
     3481 tgacggcctt ttatctctcc ggcatggtaa agtgcatcta tacctcgcgt gacgccctca
     3541 gcaagcgcct gtttcgtttc aggcaggtta tcagggagtg tcagcgtcct gcggttctcc
     3601 ggggcgttcg ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca ggcatcaatt
     3661 ctaggcctgt ctgcgcggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg
     3721 ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg
     3781 ctgtactgat ttttttcgag accgggcatc agtacacgct caaagctcgc catcactttt
     3841 tcacgtcctc ccggcggcag ctccttctcc gcgaacgaca gaacaccgga cgtgtatttc
     3901 ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt ccggattacc ctgaagcacc
     3961 gttgcgcctt cgcggttacg ctccctcccc agcaggtaat caaccggacc actgccacca
     4021 ccttttcccc tggcatgaaa tttaactatc atcccgcgcc ccctgttccc tgacagccag
     4081 acgcagccgg cgcagctcat ccccgatggc catcagtgcg gccaccacct gaacccggtc
     4141 accggaagac cactgcccgc tgttcacctt acgggctgtc tgattcaggt tatttccgat
     4201 ggcggccagc tgacgcagta acggcggtgc cagtgtcggc agttttccgg aacgggcaac
     4261 cggctccccc aggcagaccc gccgcatcca taccgccagt tgtttaccct cacagcgttc
     4321 aagtaaccgg gcatgttcat catcagtaac ccgtattgtg agcatcctct cgcgtttcat
     4381 cggtatcatt accccatgaa cagaaatccc ccttacacgg aggcatcagt gactaaacag
     4441 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gctgctggag
     4501 aagctcaacg aactggacgc agatgaacag gccgatattt gtgaatcgct tcacgaccac
     4561 gccgatgagc tttaccgcag ctgcctcgca cgtttcgggg atgacggtga aaacctctga
     4621 cacatgcagc tcccggagac ggtcacagct tgtctgtgag cggatgccgg gagctgacaa
     4681 gcccgtcagg gcgcgtcagc aggttttagc gggtgtcggg gcgcagccct gacccagtca
     4741 cgtagcgata gcggagtgta tactggctta accatgcggc atcagtgcgg attgtatgaa
     4801 aagtacgcca tgccgggtgt gaaatgccgc acagatgcgt aaggagaaaa tgcacgtcca
     4861 ggcgcttttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     4921 cggtactgac tcacacaaaa acggtaacac agttatccac agaatcaggg gataaggccg
     4981 gaaagaacat gtgagcaaaa gaccaggaac aggaagaagg ccacgtagca ggcgtttttc
     5041 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5101 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5161 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5221 gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5281 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5341 cgtcttgagt ccaacccggt aaggcacgcc ttaacgccac tggcagcagc tactggtaac
     5401 cggattagca gagcgatgat ggcacaaacg gtgctacaga gttcttgaag tagtggcccg
     5461 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5521 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgttggt agcggtggtt
     5581 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5641 tcttttctac tgaaccgcga tccccgtcag tttagaagag gaggatggtg cgatggtccc
     5701 tccctgaaca tcacgtatat agttagcctg acatccaaca aggaggttta tcgcgaatat
     5761 tcccacaaaa aatcttttcc tcataactcg atccttataa aatgaaaaga atatatggcg
     5821 aggtttaatt tatgagctta agatactaca taaaaaatat tttatttggc ctgtactgca
     5881 cacttatata tatatacctt ataacaaaaa acagcgaagg gtattatttc cttgtgtcag
     5941 ataagatgct atatgcaata gtgataagca ctattctatg tccatattca aaatatgcta
     6001 ttgaatacat agcttttaac ttcataaaga aagatttttt cgaaagaaga aaaaacctaa
     6061 ataacgcccc cgtagcaaaa ttaaacctat ttatgctata taatctactt tgtttggtcc
     6121 tagcaatccc atttggattg ctaggacttt ttatatcaat aaagaataat taaatcccta
     6181 acacctcatt tatagtatta agtttattct tatcaatata ggagcataga attcctgtaa
     6241 caatagcaat accccaaata cctaatgtag ttccagcaag caagctaaaa agtaaagcaa
     6301 caacataact cacccctgca tctgcagctt tcttctctaa tgtaagaaat agtggcttcc
     6361 agtcacctgt atccttaatt tttaggatat cagataccac atcatatcca aaagaaacat
     6421 gccccgtaat cttcaagtac ttggcaaact gatctaaatg tttagcccag tcatcatact
     6481 tcaccgatgc caacgcatta aaaatagcat cacgatcggc tttgctgaat ttcttattta
     6541 aaacatcctt gtatttttca aaagcagcga gagcttcatt cacattgccg attttcttac
     6601 ctttagactt atcagcaagt tcctgtgcca ttttcgaata tttttca
//
