LOCUS       NZ_CP055604             6647 bp    DNA     circular CON 01-APR-2024
DEFINITION  Escherichia fergusonii strain RHB41-C23 plasmid pRHB41-C23_6,
            complete sequence.
ACCESSION   NZ_CP055604
VERSION     NZ_CP055604.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148431
            Assembly: GCF_013591455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia fergusonii
  ORGANISM  Escherichia fergusonii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T3-C23
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145.857560681824x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013591455.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 04:10:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,801
            CDSs (total)                      :: 4,683
            Genes (coding)                    :: 4,563
            CDSs (with protein)               :: 4,563
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 26 of 120
            Pseudo Genes (incomplete)         :: 84 of 120
            Pseudo Genes (internal stop)      :: 18 of 120
            Pseudo Genes (multiple problems)  :: 10 of 120
            Pseudo Genes (short protein)      :: 2 of 120
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055604.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia fergusonii"
                     /mol_type="genomic DNA"
                     /strain="RHB41-C23"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:564"
                     /plasmid="pRHB41-C23_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6421..6647,1..7)
                     /locus_tag="HV014_RS23915"
                     /old_locus_tag="HV014_23750"
     CDS             join(6421..6647,1..7)
                     /locus_tag="HV014_RS23915"
                     /old_locus_tag="HV014_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(325..744)
                     /locus_tag="HV014_RS23755"
                     /old_locus_tag="HV014_23755"
     CDS             complement(325..744)
                     /locus_tag="HV014_RS23755"
                     /old_locus_tag="HV014_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(825..1262)
                     /locus_tag="HV014_RS23760"
                     /old_locus_tag="HV014_23760"
     CDS             complement(825..1262)
                     /locus_tag="HV014_RS23760"
                     /old_locus_tag="HV014_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_181199454.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMFEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1522..3090
                     /gene="cea"
                     /locus_tag="HV014_RS23765"
                     /old_locus_tag="HV014_23765"
     CDS             1522..3090
                     /gene="cea"
                     /locus_tag="HV014_RS23765"
                     /old_locus_tag="HV014_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(3087..3428)
                     /locus_tag="HV014_RS23770"
                     /old_locus_tag="HV014_23770"
     CDS             complement(3087..3428)
                     /locus_tag="HV014_RS23770"
                     /old_locus_tag="HV014_23770"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3476..3613
                     /locus_tag="HV014_RS23775"
                     /old_locus_tag="HV014_23775"
     CDS             3476..3613
                     /locus_tag="HV014_RS23775"
                     /old_locus_tag="HV014_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3812..3970)
                     /locus_tag="HV014_RS24300"
     CDS             complement(3812..3970)
                     /locus_tag="HV014_RS24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4640..4831)
                     /locus_tag="HV014_RS23780"
                     /old_locus_tag="HV014_23780"
     CDS             complement(4640..4831)
                     /locus_tag="HV014_RS23780"
                     /old_locus_tag="HV014_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4895..5218
                     /locus_tag="HV014_RS23785"
                     /old_locus_tag="HV014_23785"
     CDS             4895..5218
                     /locus_tag="HV014_RS23785"
                     /old_locus_tag="HV014_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5427..>5858
                     /locus_tag="HV014_RS24170"
                     /pseudo=""
     CDS             5427..>5858
                     /locus_tag="HV014_RS24170"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5896..6414
                     /locus_tag="HV014_RS23925"
                     /old_locus_tag="HV014_23795"
     CDS             5896..6414
                     /locus_tag="HV014_RS23925"
                     /old_locus_tag="HV014_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     Typing          4468..4555
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3899..4454)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5209..6626
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.789"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 gagataagag aggaacggct gatacaggag cgcgaacatg tgttatccct ggaacgggag
       61 cgtcagccgg aaatacagga acgcacgctg gatggccctt cgctgggatg gtgaaaccat
      121 gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc tctggttgca
      181 taggtattca tacggttaaa atttatcagg cgcgatcgcg gcagtttttc gggtggtttg
      241 ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc agtgcgtaca
      301 attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga gataaaccgc
      361 agaaatcgtg tcagccagca gcctggattt tttcggggta aggtctttgc tgcggtaatc
      421 agtacccgca aattgagttg tgccccggag tgagtttaaa aattcctggc tgtgaaactg
      481 ctgaatgcgt tgttgcacat cactccgtga ttttcctgtc gctttatctt tagctcccat
      541 atcccagaac tgccgaaaaa aaggcaccat cattcgtgtt gtgtcggctt tttgggtgga
      601 aaagttggga tcaaaaccgt catcagaagc atatacgtaa tgccgggtat gttgttccac
      661 gctgcccttt ggggtacagg ctgtgagagc tactgttgct gttatcatgg cgataagaca
      721 aagtttgttt ccggtacgtc tcatgagtgt ctccctacct taaagtattt attctcggtg
      781 agttattatc cgaagactat tttatcatca gtctctgaca gcgttcagcg accgaacaac
      841 ctggaccaga atccccgttt cttttccagt tccagtgcct gcctgagctg ggctatctca
      901 ttttgtagct gttcccgttc tgcttcctgg cgtctgcgat ccatatcctg tgcctgttta
      961 tcctcaaaca tcagcgtcag gcattgtttc agctcattca gctcccggag aatgcgttct
     1021 gtctgctgat catgtggatt ttctgcatgt ccctcactgt gcctttctgg tgtctcattc
     1081 tgctttaatt cgccgtatgc ccggatcagt tcactggttt caaactcccg tcgtccgtcc
     1141 ctgccggtac ggtaactgac aaggcctgcg cgcatatcac ggtaaaactg tgaacgcgat
     1201 ctgcctgtca tttttagtgc gtcccggagt gtgtgccatg ccataaagtg acagtgtccc
     1261 atagatgtct catctcatag tttcagtaaa acataatgag gtctgagaac ggtaatgttt
     1321 gtgctggttt ttgtggcatc gggcgagaat agcgcgtggt gtgaaagact gtttttttga
     1381 tcgttttcac aaaaatggaa gtccacagtc ttgacaggga aaatgcagcg gcgtagcttt
     1441 tatgctgtat ataaaaccag tggttatatg tacagtattt atttttaact tattgtttta
     1501 aaagtcaaag aggattttat aatggaaacc gcggtagcgt actataaaga tggtgttcct
     1561 tatgatgata agggacaggt aattattact cttttgaatg gtactcctga cgggagtggc
     1621 tctggcggcg gaggtggaaa aggaggcagt aaaagtgaaa gttctgcagc tattcatgca
     1681 actgctaaat ggtctactgc tcaattaaag aaaacacagg cagagcaggc tgcccgggca
     1741 aaagctgcag cggaagcaca ggcgaaagca aaggcaaaca gggatgcgct gactcagcgc
     1801 ctgaaggata tcgtgaatga ggctcttcgt cacaatgcct cacgtacgcc ttcagcaaca
     1861 gagcttgctc atgctaataa tgcagctatg caggcggaag cagagcgttt gcgccttgcg
     1921 aaagcagaag aaaaagcccg taaagaagcg gaagcagcag aaaaggcttt tcaggaagca
     1981 gaacaacgac gtaaagagat tgaacgggag aaggctgaaa cagaacgcca gttgaaactg
     2041 gctgaagctg aagagaaacg actggctgca ttgagtgaag aagctaaagc tgttgagatc
     2101 gcccaaaaaa aactttctgc tgcacaatct gaagtggtga aaatggatgg agagattaag
     2161 actctcaatt ctcgtttaag ctccagtatc catgcccgtg atgcagaaat gaaaacgctc
     2221 gctggaaaac gaaatgaact ggctcaggca tccgctaaat ataaagaact ggatgagctg
     2281 gtcaaaaaac tatcaccaag agccaatgat ccgcttcaga accgtccttt ttttgaagca
     2341 accagacgac gggttggggc cggtaagatt agagaagaaa aacaaaaaca ggtaacagca
     2401 tcagaaacac gtattaaccg gataaatgct gatataactc agatccagaa ggctatttct
     2461 caggtcagta ataatcgtaa tgccggtatc gctcgtgttc atgaagctga agaaaatttg
     2521 aaaaaagcac agaataatct ccttaattca cagattaagg atgctgttga tgcaacagtt
     2581 agcttttatc aaacgctgac tgaaaaatat ggtgaaaaat attcgaaaat ggcacaggaa
     2641 cttgctgata agtctaaagg taagaaaatc ggcaatgtga atgaagctct cgctgctttt
     2701 gaaaaataca aggatgtttt aaataagaaa ttcagcaaag ccgatcgtga tgctattttt
     2761 aatgcgttgg catcggtgaa gtatgatgac tgggctaaac atttagatca gtttgccaag
     2821 tacttgaaga ttacggggca tgtttctttt ggatatgatg tggtatctga tatcctaaaa
     2881 attaaggata caggtgactg gaagccacta tttcttacat tagagaagaa agctgcagat
     2941 gcaggggtga gttatgttgt tgctttactt tttagcttgc ttgctggaac tacattaggt
     3001 atttggggta ttgctattgt tacaggaatt ctatgctcct atattgataa gaataaactt
     3061 aatactataa atgaggtgtt agggatttaa ttattcttta ttgatataaa aagtcctagc
     3121 aatccaaatg ggattgctag gaccaaacaa agtagattat atagcataaa taggtttaat
     3181 tttgctacgg gggcgttatt taggtttttt cttctttcga aaaaatcttt ctttatgaag
     3241 ttaaaagcta tgtgttcaat agcatatttt gaatatggac atagaatagt gcttatcact
     3301 attgcatata gcatcttatc tgacacaagg aaataatacc cttcgctgtt ttttgttata
     3361 aggtatatat atataagtgt gcagtacagg ccaaataaaa tattttttat gtagtatctt
     3421 aagctcataa attaaacctc gccatatatt cttttcattt tataaggatc gagttatgag
     3481 gaaaagattt tttgtgggaa tattcgcgat aaacctcctt gttggatgtc aggctaacta
     3541 tatacgtgat gttcagggag ggaccatcgc accatcctcc tcttctaaac tgacggggat
     3601 cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3661 tgctgcttgc aaacaaaaaa accaccgcta ccaacggtgg tttgtttgcc ggatcaagag
     3721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3781 cttctagtgt agccgtagtc gggccactac ttcaagaact ctgtagcacc gtttgtgcca
     3841 tcatcgctct gctaatccgg ttaccagtgg ctgctgccag tggcgttaag gcgtgcctta
     3901 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3961 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga taccaacagc
     4021 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4081 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4141 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4201 caggggggcg gagcctatgg aaaaacgcct gctacgtggc cttcttcctg ttcctggtct
     4261 tttgctcaca tgttctttcc ggccttatcc cctgattctg tggataactg tgttaccgtt
     4321 tttgtgtgag tcagtaccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc
     4381 gaggaagcgg aaaagcgcct ggacgtgcat tttctcctta cgcatctgtg cggcatttca
     4441 cacccggcat ggcgtacttt tcatacaatc cgcactgatg ccgcatggtt aagccagtat
     4501 acactccgct atcgctacgt gactgggtca gggctgcgcc ccgacacccg ctaaaacctg
     4561 ctgacgcgcc ctgacgggct tgtcagctcc cggcatccgc tcacagacaa gctgtgaccg
     4621 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc cccgaaacgt gcgaggcagc
     4681 tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca aatatcggcc tgttcatctg
     4741 cgtccagttc gttgagcttc tccagcagcg ttaatgtctg gcttctgata aagcgggcca
     4801 tgttaagggc ggttttttcc tgtttagtca ctgatgcctc cgtgtaaggg ggatttctgt
     4861 tcatggggta atgataccga tgaaacgcga gaggatgctc acaatacggg ttactgatga
     4921 tgaacatgcc cggttacttg aacgctgtga gggtaaacaa ctggcggtat ggatgcggcg
     4981 ggtctgcctg ggggagccgg ttgcccgttc cggaaaactg ccgacactgg caccgccgtt
     5041 actgcgtcag ctggccgcca tcggaaataa cctgaatcag acagcccgta aggtgaacag
     5101 cgggcagtgg tcttccggtg accgggttca ggtggtggcc gcactgatgg ccatcgggga
     5161 tgagctgcgc cggctgcgtc tggccgtcag ggaacagggg gcgcgggatg atagttaaat
     5221 ttcatgccag gggaaaaggt ggtggcagtg gtccggttga ttacctgctg gggagggagc
     5281 gtaaccgcga aggcgcaacg gtgcttcagg gtaatccgga agaagtccgg gaactcatcg
     5341 atgccacgcc atttgcgaag aaatacacgt ccggtgttct gtcgttcgcg gagaaggagc
     5401 tgccgccggg aggacgtgaa aaagtgatgg cgagctttga gcgtgtactg atgcccggtc
     5461 tcgaaaaaaa tcagtacagc atcctgtggg tggagcacca ggacaaggga cggcttgagc
     5521 tgaattttgt cattccgaac atggagctac agaccggaaa acgcctccag ccgtactacg
     5581 accgcgcaga caggcctaga attgatgcct ggcagacgct ggtgaatcac cattacgggc
     5641 tgcatgaccc gaacgccccg gagaaccgca ggacgctgac actccctgat aacctgcctg
     5701 aaacgaaaca ggcgcttgct gagggcgtca cgcgaggtat agatgcactt taccatgccg
     5761 gagagataaa aggccgtcag gatgtgattc aggcgctcac tgaggcgggg ctggaagtgg
     5821 tcagggtgac gcgaagcagt atcagcattg cagatccgaa cggcgggaag aatatcaggc
     5881 tgaaaggagc attttatgag caatcttttg cagacgggcg cggagttcga gaaaaagctg
     5941 aaagagagag ccgaatctac agagaaaatg ctgaacaacg agttcaggag gctcggcgaa
     6001 tctgtaagcg aggctgtgac atcaaacgag acgaaaatca gagacgctat agccctgttc
     6061 acagcctcga cagaggaatc gctggaaaaa caccgggaag gggtgaaaga ggcgatgatg
     6121 cagcacagga gggacgtgtt aaagctggca gggaatacgg gcatgatgtt actggggata
     6181 gtctttctcc tgtttaccgc gagtggcggg acgctctggt atcttggagg gaggatacag
     6241 gcgaacctgg aagaaatcag gaagcaggaa gagacattgc agaaactgaa cgcgaagaca
     6301 tggggcgtgg agtttgtgca ggacgggaac aggaaattcc ttgtccttcc gtacgggaaa
     6361 tcagcggagg tgattccctt tcaggggaaa gagtgggtac atctgaagga gtgacacaga
     6421 gtgacagagc tggaaacaca tttgctgaac gccttagagc agctgcaaca ggactatatg
     6481 cagcggctga gcgaatggga gagcgccttc gtggaattgc agaagatgtt ttcgcttacg
     6541 caacgggaca acgcgatgct gaacgagcgg gtcatgcagt tgagtcagca ggtgcagcac
     6601 ttgagcgagc agacagaacg cttgagccag ttatacagcg agaactg
//
