LOCUS       NZ_CP055592             7536 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00084 plasmid pRHBSTW-00084_5,
            complete sequence.
ACCESSION   NZ_CP055592
VERSION     NZ_CP055592.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148477
            Assembly: GCF_013590555.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00084
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.684480964081x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 13:53:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,334
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 5,088
            CDSs (with protein)               :: 5,088
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 35 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 27 of 124
            Pseudo Genes (multiple problems)  :: 21 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055592.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00084"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00084_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            131..622
                     /locus_tag="HV060_RS26550"
                     /old_locus_tag="HV060_26550"
     CDS             131..622
                     /locus_tag="HV060_RS26550"
                     /old_locus_tag="HV060_26550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032072598.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQNMSEAMEKNRRSVLLTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            629..844
                     /locus_tag="HV060_RS26555"
                     /old_locus_tag="HV060_26555"
     CDS             629..844
                     /locus_tag="HV060_RS26555"
                     /old_locus_tag="HV060_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1413..2045
                     /locus_tag="HV060_RS26560"
                     /old_locus_tag="HV060_26560"
     CDS             1413..2045
                     /locus_tag="HV060_RS26560"
                     /old_locus_tag="HV060_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001567369.1"
                     /translation="MLRYELTPNNAGFILWGDSEALNELHELIHYIVDESPLIKVKDGF
                     MLSLAYDIRKAREGNRRVEQHQYDQHDTYKLYGVELLWPLVLVQSSILRNSMGYIQTDK
                     NQLSVMYAFEYLIESALTESERTTSNDIMLTVKYASDSDFNFIEDNIDSRCCYFISLSP
                     EQRKKQLISIVRSFHSLWGKYAREKQDIKMLNEMNNTSWVWPDNINW"
                     /name="hypothetical protein"
     gene            complement(2074..3477)
                     /locus_tag="HV060_RS26565"
                     /old_locus_tag="HV060_26565"
     CDS             complement(2074..3477)
                     /locus_tag="HV060_RS26565"
                     /old_locus_tag="HV060_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element ISKpn21 family transposase"
                     /protein_id="WP_001567368.1"
                     /translation="MSAESSGVFTLKEINRIKIIQDVIERRITTRRAAEHLGISDRQCR
                     RLLARYREGGPLGMASRRCGMRGNRQLPPGLADQALELIKTRYADFGPTLAREKLEELH
                     GLFLGKETVRRIMVRAGLWVPRKQRAARIPQPRYRRPCTGELIQIDGCDHDWFEGRGPA
                     CTALVYVDDATSKLMELLFVKSESTFSYFEATRRYIDKHGKPLALYSDKAGVFRVNNKH
                     ATGGDGHTQFGRAMHELNIQTICAETSPAKGRVERAHLTLQDRLVKELRLQGICSMEAA
                     NDFAEAYMADYNRRFGKVPRHDFDVHRAVEHDEDLGLIFTVREKRKVSKSLTIQYDKML
                     YLIEDSELSRRAIGKYIDVYHYPDGRKELRLNGTLLPYSTYDRLSEIDQGAIVDNKRLG
                     RTLEFISLVQSKRDNTRSQSIPAGDGPSRRRPKQEGKKSQRSLDNDDMLEALKQLQSRS
                     EDIFGKRAR"
                     /name="ISNCY-like element ISKpn21 family transposase"
     gene            complement(3656..4087)
                     /locus_tag="HV060_RS26570"
                     /old_locus_tag="HV060_26570"
     CDS             complement(3656..4087)
                     /locus_tag="HV060_RS26570"
                     /old_locus_tag="HV060_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_032623639.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKNGRLSSSDDFPVSRSA
                     DNHELTHHIELMKKELKENQEYLDESFKRLSAELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(4789..5694)
                     /locus_tag="HV060_RS26575"
                     /old_locus_tag="HV060_26575"
     CDS             complement(4789..5694)
                     /locus_tag="HV060_RS26575"
                     /old_locus_tag="HV060_26575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_258042123.1"
                     /translation="MWKGCWLTTMPSTGTTEQKNDKTCILGLFLQTHNRIKYFFYLIGL
                     SNEIHHIGKNFHHCSFYFIPLFNLCFNKDYWCMKMQHNSSDIDLIELFLFLKTKIASII
                     LFVIFSLILSFTFLFITKDKVIIKYQLNMINNSPSMIINCGDDFYCKANAIQSIINNKS
                     KSITSSIDEKAQKINLSWSGDVRDKPVLIAEVNTIHRAIDDWYIQDYQNYKSIVNNQSN
                     NYINGSEIYAKIALLTNSNTAGENKFISISNETVSKSYKPALFMTLSLILSVLFSSAYH
                     ITKRSVLEYKNKFNRSFDNL"
                     /name="hypothetical protein"
     gene            complement(5905..6018)
                     /locus_tag="HV060_RS26580"
                     /old_locus_tag="HV060_26580"
     CDS             complement(5905..6018)
                     /locus_tag="HV060_RS26580"
                     /old_locus_tag="HV060_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045907702.1"
                     /translation="MHEPPVQSDCCALSGNYQLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     gene            6666..6989
                     /locus_tag="HV060_RS26585"
                     /old_locus_tag="HV060_26585"
     CDS             6666..6989
                     /locus_tag="HV060_RS26585"
                     /old_locus_tag="HV060_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181213546.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVIEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(6958..7416)
                     /locus_tag="HV060_RS26885"
     CDS             complement(6958..7416)
                     /locus_tag="HV060_RS26885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_326929814.1"
                     /translation="MQPQSAVHDSLPGIDARPVGAVVVRLEPPAGQQLGIGDQKVQLQT
                     PLVRVLHPQNAVLIFIESGHQNSLEAGHQFFPLAGREIGLRKRQDAGGVFLNVRRRVNQ
                     LPDFFRLPLQDGGAFTLPVFTQQIVHRPGATAATAWMKFNDHRPFLSQ"
                     /name="hypothetical protein"
     Typing          6380..6553
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(5910..6456)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.091"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2..210
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.571"
                     /coverage="92.0"
                     /evalue="2.3300000000000002e-29"
     Typing          6667..6983
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="4.29e-61"
     Typing          6980..7536
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.462"
                     /coverage="100.0"
                     /evalue="9.64e-116"
ORIGIN
        1 ctgaaaagcc gtcaggatgt cactgacact cttgaaggtg caggttttga ggtggtgcgc
       61 accacgaaaa gcagtatcag cattgccgac ccggacgggg ggcgaaacat ccgactgaag
      121 ggagctatct atgagcaatc tttttacggc ctcgagggac ttcgaggaga aattgaaagc
      181 gcagcagcag agtaccggcg agatgctgaa agccgcattc agcgagcacg agcagtctgt
      241 cagagcggaa ctgagcgcaa gcgcgaggag aatcagcgac gccatcagcg cccacgagca
      301 gaatatgagc gaggcgatgg agaaaaaccg gcggagcgtg ctgctgacgg ccgggaggac
      361 atggctcacc atcctgatgg tgtcagcgct gctgatcggc acgagcggga gcattctgtg
      421 gtggcagggg cagcagataa ccgacaatta cacgcatctt cgtcagcagg aagataccct
      481 ggcgaaaatg accgcccgga cgtggggagt gcgctatcag gagagcagcg acggccggag
      541 attccttatc ctgccgccgg ggatgcaaac cgaagccatc ccttacgacg ggacgacatg
      601 gatccgcctg aaacaggagt agcggaaaat gacagagctg gaaaagcagt tgctgagcgc
      661 attagagcag ctacagcagg actactcgca aaggctggac gaatgggaga gcgccttcgc
      721 ggaatggcgg agcatgtgtg gtcttatgca acgggagaac gcgatgctga acgagcgcgt
      781 gtcggacttg agcacgcagg tgctgagttt aagcgagcag ctgcgccgct tgtccgggaa
      841 ctgaatgcga tcgagctccg gcagagggag aatgagtatc agaaaacact ggagctggag
      901 cgccagccgc agaaaaccta ccgcgggccg acactttaaa ccatccaggc agctgacaac
      961 ccgcacgaag aggggctctt atttctcccg ctaaataggt tcgtgatcaa aaaggtcacg
     1021 acttattcag gagcgggaaa tgaagctgtt aaatcttatt gaatcattgc ttaactgcct
     1081 atacctggcg gcggagctgt gggactggct ggagaagcat ttgtcggttt ttaaggcatt
     1141 ggcgcggatt ttagtgtgac tttaacatcc atgcatctgg ctgcatgact atgcaccagt
     1201 gtaatattca gcggctgcgt ttctggtgtt ttttcgttat gtctgtcgcc actttctcgc
     1261 gtctggcacc ggaaacgcag ccaaccccgt ttcagttgtc aaggctcaat tgaaatgtcc
     1321 gttatccagc tcaattaaaa tgaccacttt gatctctcat tctctttact gatagacttt
     1381 cctccgactg aaacaacagg atgattgagc ccatgcttcg atacgagtta acgccgaaca
     1441 atgcaggttt tatactgtgg ggagattcag aagccctgaa tgaattacat gaactcattc
     1501 attacatcgt ggatgaaagc ccactgatta aagttaaaga cggatttatg ttatcccttg
     1561 cctatgatat tcgtaaagca cgggaaggta atcgtcgtgt tgagcaacat cagtatgatc
     1621 aacatgatac atataagctt tatggtgttg agcttttatg gcctctggtc ctggtacagt
     1681 cctcaatact cagaaactca atgggttata ttcagacaga caaaaaccag ctgtctgtca
     1741 tgtatgcctt tgaatacctg atagaatcag cattaacaga gtctgagaga acaacgtcga
     1801 atgatattat gctaacagta aaatatgcat cagactctga ttttaatttc attgaggata
     1861 atattgacag caggtgctgc tattttatca gcctatctcc ggagcaaaga aaaaagcagt
     1921 taatcagtat tgttcgttct tttcattcat tatggggtaa gtatgcccgt gaaaagcagg
     1981 acataaagat gctgaacgaa atgaataata catcatgggt ctggccggac aatatcaact
     2041 ggtgagcaac cactgtccgg ccagtgagta ccatcagcgg gctcttttac caaaaatgtc
     2101 ctctgaacgt gactgaagct gtttgagtgc ttcgagcatg tcatcattat ccagtgagcg
     2161 ctgggatttc ttcccttcct gctttggccg tcgtcgggaa gggccatctc cagcgggaat
     2221 tgactgagag cgcgtgttat cccgcttgct ctgcaccaga ctgataaact ccagggttcg
     2281 gccaagacgc ttgttatcga caatcgcgcc ctggtcgatt tctgacagtc ggtcgtaggt
     2341 agagtaggga agtagcgtac cgttcaggcg cagctctttt ctgccatcag gatagtgata
     2401 cacatcgata tatttaccta ttgcacggcg actcagttcg ctgtcttcaa tcaggtacaa
     2461 cattttatca tattgtatcg tcaacgattt tgagacttta cgtttttcac gaacagtgaa
     2521 aataagcccc aggtcctcat catgttctac agcacggtgt acgtcaaaat catgtcgcgg
     2581 tactttgcca aaacggcggt tatagtcagc catataggcc tcagcgaagt catttgcagc
     2641 ctccattgaa caaatgccct gtaaccgcag ctctttgacc agacgatcct gtaaagtgag
     2701 gtgagctcgt tctacacgcc ctttggcggg actggtttct gcacagatag tctggatgtt
     2761 cagttcatgc atggctcgcc caaactgagt atgcccgtct ccgcctgtgg cgtgtttatt
     2821 gttaacacga aaaacaccgg ctttatcgct gtacagtgcc agcggtttac catgcttatc
     2881 gatatagcgc cgcgtggctt cgaagtaaga aaacgtggac tccgatttaa caaacaacag
     2941 ttccatcagt ttgctggttg catcatcaac atagaccagc gcggtgcagg ccgggccacg
     3001 gccttcaaac cagtcgtgat cacagccatc tatttgtatc agctcaccag tacacggacg
     3061 ccggtaccgt ggttgaggga tccttgcggc acgttgttta cggggaaccc ataagccagc
     3121 ccgcaccatg atgcgccgga cagtttcttt gccaagaaac agtccgtgga gttcttcgag
     3181 cttttcacgc gccagagtcg gaccgaaatc agcataacgc gtcttgatca gttccagagc
     3241 ctgatctgcg agcccgggtg gcaactggcg gttaccacgc atgccacatc gtctgctggc
     3301 cataccaagc ggtccgcctt cacggtaacg ggcaagaagt ctgcggcatt gcctgtcgct
     3361 gataccgagg tgctcggccg cacggcgcgt tgtgatgcga cgttcaatga cgtcctgtat
     3421 aatcttgatc cggttgatct ctttcaaagt aaacactcct gagctttctg cgctcatgat
     3481 atgcctcccg gtagtttaaa cggaccagtg aagcttacca tagcgcggac atctgaattg
     3541 agccacaggc ggacattact attgagccat tacatcagtg gtgcgggcaa cagatgttat
     3601 ggtaaatacg gcggtgctgc agacacaaaa aagccgccca tctgggcggc tttttttagt
     3661 agccaagacg tttcaattca gccgaaaggc gtttaaagct ctcgtcgaga tattcctgat
     3721 tttcttttaa ctcttttttc attagttcga tgtgatgagt gagttcatgg ttgtctgcag
     3781 aacgactgac cgggaagtcg tcactggatg atagccgccc atttttgctg taatccatcg
     3841 ctatgcagat cagagtggca aggtctatta ctcggtgaag tggaagctcc tcagactgac
     3901 gggaccactt ctcgccggta tttcgccaaa cctttgccga aagctctgtc catcccgggg
     3961 tgttccattg tgcgatacca acagataacc ccattgcatc agtgtcgtcg gcatatggtc
     4021 catcaatgcg atcataattc tctacttgaa taattggctt atgtttcagg tgggcgggga
     4081 ttttcattct ctatgtctct ctcatttatt tgttgatttg tagattatat tgttttacta
     4141 attagtaaat ttactaaatg taacaaaatg agaattctag gttatttcat tgttttgatt
     4201 gatttttttg atggatgagt ctgagatggt taactggtat atacggctaa tagcgggcgt
     4261 tgaagagtgt ggataaagct ctaaatggtg ctccatccag agggcgcaat acggatgtta
     4321 tggtaaattt cggtgcattg tgactcgttt ggatatgtta tttggttgca gtattgaacg
     4381 acccagagga ttaaaaatgg atctagcaaa agcactgaag gaagcggaaa ccggatatag
     4441 cctcaactta accagtacag atgaggctga aatgctggct tatatctcaa ccgtacttga
     4501 actccacgct aaaggtgagc tactacatga gtctgccata gacagggcta tgacgttatg
     4561 tgtgcttatt aacaaaggaa accgacagga gttaagccgg acgcttcgcg ggtttaacaa
     4621 tggtggtaac tggcctagca ctacgggagc ttcgtcaggc tgcggaaggg gaaacgctta
     4681 gcgctgaaat cccggaggta tttaagatct tatcgaaaag cccactgata gtaggctttt
     4741 ctgcatctgg cgtatatcgg atgttatgag gaatatggtt attaaccgtt ataggttatc
     4801 aaaagaccgg ttgaatttgt ttttatattc aagcacagat cttttggtta tatgatatgc
     4861 ggatgaaaaa agaacagaaa gaattaaaga tagagtcata aacaaggctg gtttgtaact
     4921 tttgcttacc gtttcattgc tgattgaaat gaatttattt tctccagcag tgttgctgtt
     4981 tgttaacaat gcaatcttcg catatatctc agaaccattt atatagttgt tactctgatt
     5041 gttaactatg cttttataat tttgataatc ttggatgtac caatcatcaa tagctcgatg
     5101 gatagtgttt acttcagcga ttaaaacagg tttgtcacgg acgtcgccag accatgaaag
     5161 gtttattttt tgtgcttttt catctatgct tgatgttatg ctcttgcttt tgttgtttat
     5221 tattgattgt atggcgttcg ctttacaata aaaatcatcc ccacaattaa taatcatgct
     5281 gggtgaattg ttgatcatgt ttaattggta ttttatgatt actttgtctt ttgttataaa
     5341 caggaaggta aatgacaaaa ttaatgagaa aataacaaat aaaatgatgc tcgcaatttt
     5401 tgtcttaaga aagagaaata gctctattaa atcaatatcc gatgaattgt gttgcatttt
     5461 catacaccaa tagtctttat taaagcataa gttaaataaa ggaatgaaat aaaaagagca
     5521 gtgatgaaaa tttttgccga tgtggtgtat ttcatttgaa aggccaataa gataaaaaaa
     5581 atattttatc ctattatgtg tttgtaggaa aagtcctaaa atgcaagttt tgtcgttttt
     5641 ttgttcggtt gtgcctgtgg agggcattgt tgtcagccaa catcctttcc acatatttta
     5701 agatcttctt gagatccttg cgttacagcc gtaatctctt gctctgaaaa cgaaaaaacc
     5761 acctgccagg gtggtttttt cgaaggttct ctgagctacc aactctttga accgaggtaa
     5821 ctggcttgga ggagcacagt caccaaaact gttcgtttag tttagcctta accggcgcat
     5881 aacttcaaga ctaactcctc taaatcagtt accagtggct gctgccagtg gtgctttgtc
     5941 atgtctttcc gggttggact caagttgata gttaccggat aaggcgcagc agtcggactg
     6001 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     6061 caggcgtgga atgagataaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     6121 caggaacagg agagcgcacg agggagccac cagggggaaa cgcctggtat ctttaagtcc
     6181 tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg
     6241 gagcctatag aaaaacggct ccgccgcggc cttatcgctt ccctgttaag tctcttcttg
     6301 gcatctccta ggaaatttcc gccccgccag taagccggta ccgctcgccg cagccgaacg
     6361 accgagcgca gcgagtcagt gagcgaggaa gcggaatata tcctgtatcg catattctgc
     6421 ttacgcgccg gtgccgcctt ttttctcctt ccacatgaag cacttcactg atttccgcat
     6481 ccgtgccaac atagtaagcc agtatacact ccgctagcgc tacgtgactg gttcagggct
     6541 gcgccccgaa acccgccaaa agcctgacgc gcctgcggct tgtccaaccc cgcgccgacc
     6601 gggaaaggct ttcccgcccg tcccggcgtt atcaggaggc cggtttgtca gagaaacgga
     6661 acaagatgct caccatgtgg gtgacggagg acgagcaccg gcggctgctt gagcgctgcg
     6721 acggcaggca gctggcagcg tggatgcggc agacctgcct ggacgaaaag ccggcacgtt
     6781 ccggaaagct tccctcaatc tcgccggcgc tgctgcgcca gcttgccggc atgggcaata
     6841 acctgaacca gatagcccgc cgtgttaacg ccggcggggg taccggccat gacagggtgc
     6901 agattgttgc ggcgctgatg gccatcgatg ccggacttga gcggctgcgg cacgccgtta
     6961 ttgagaaagg aacggacgat gatcgttaaa tttcatccac gcggtcgcgg cggtggcgcc
     7021 gggccggtgg actatctgct gggtaaagac cggcagcgtg aaggcgccac cgtcctgcag
     7081 gggaagccgg aagaagtccg ggagctgatt gacgcgtcgc cttacgttaa gaaatacacc
     7141 tccggcgtcc tgtcgtttgc ggaggccgat ctccctcccg gccagcggga aaaactgatg
     7201 gccagcttcg agcgagttct gatgcccgga ctcgataaag atcagtacag cattctgtgg
     7261 gtggagcacg cggacaaggg gcgtctggag ctgaactttc tgatccccaa taccgagctg
     7321 ctgaccggca ggcggctcca gccttactac gaccgcgccg accggccgcg catcgatgcc
     7381 tggcagacta tcgtgaacgg cagactgggg ctgcacgacc cgaacgcgcc ggaaaaccgc
     7441 cgggcgctgg tcacgccgtc cgggctgccg aaaacgaagc aggaggccgc agaagccatt
     7501 acccggggac tactgagctt agcctcgtcc ggggag
//
