LOCUS       NZ_CP055591            12871 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00084 plasmid pRHBSTW-00084_4,
            complete sequence.
ACCESSION   NZ_CP055591
VERSION     NZ_CP055591.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148477
            Assembly: GCF_013590555.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 12871)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00084
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.684480964081x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 13:53:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,334
            CDSs (total)                      :: 5,212
            Genes (coding)                    :: 5,088
            CDSs (with protein)               :: 5,088
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 35 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 27 of 124
            Pseudo Genes (multiple problems)  :: 21 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055591.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00084"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00084_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(12810..12871,1..1228)
                     /locus_tag="HV060_RS26470"
                     /old_locus_tag="HV060_26470"
     CDS             join(12810..12871,1..1228)
                     /locus_tag="HV060_RS26470"
                     /old_locus_tag="HV060_26470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181213543.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            1225..1866
                     /locus_tag="HV060_RS26475"
                     /old_locus_tag="HV060_26475"
     CDS             1225..1866
                     /locus_tag="HV060_RS26475"
                     /old_locus_tag="HV060_26475"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_181213544.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKATKSEPQPAERTTSKAKVQPK
                     VKTPQKPAQEPTAAKAKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQC
                     LIDQCMRYDLDGNPTVAVTPEEAEQAKTRLTEIREFRRKARQEKDKKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(1886..2071)
                     /locus_tag="HV060_RS26480"
                     /old_locus_tag="HV060_26480"
     CDS             complement(1886..2071)
                     /locus_tag="HV060_RS26480"
                     /old_locus_tag="HV060_26480"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151322523.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRRELPRSTGR"
                     /name="hypothetical protein"
     gene            complement(2064..2426)
                     /locus_tag="HV060_RS26485"
                     /old_locus_tag="HV060_26485"
     CDS             complement(2064..2426)
                     /locus_tag="HV060_RS26485"
                     /old_locus_tag="HV060_26485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061301020.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPFLHHHPGDRPAT
                     EKRAIARFKDSLAAGGQTLAFVRSDASLYALGLRFRRERTRELVASDPDVAAAGEALEQ
                     RANGVNLSYTFGGGDE"
                     /name="hypothetical protein"
     gene            complement(2419..2808)
                     /locus_tag="HV060_RS26490"
                     /old_locus_tag="HV060_26490"
     CDS             complement(2419..2808)
                     /locus_tag="HV060_RS26490"
                     /old_locus_tag="HV060_26490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015768.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            complement(2991..3650)
                     /locus_tag="HV060_RS26495"
                     /old_locus_tag="HV060_26495"
     CDS             complement(2991..3650)
                     /locus_tag="HV060_RS26495"
                     /old_locus_tag="HV060_26495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548402.1"
                     /translation="MTEIKKESADGAWLRSTLAELAGDAFPVYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            3901..4476
                     /locus_tag="HV060_RS26500"
                     /old_locus_tag="HV060_26500"
     CDS             3901..4476
                     /locus_tag="HV060_RS26500"
                     /old_locus_tag="HV060_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151785.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_101998997.1"
                     /translation="MSRVFGYGRVSTGEQTVAGQKKAILDRYPQATFHADEGISGTVPA
                     TQRDKFKAILEFAAPGDSIVVVAIDRIGRNARSVMEVCEELEKRQVSLISLREGFDFST
                     PTGKMMLTMVAAFAEMELATLKERTKAGMAAAAAAGRHAGRPTKQTPENVGAVIMKHDA
                     GVSAARIARDLGISRGTVNRILQKHAKA"
                     /name="recombinase family protein"
     gene            4488..5075
                     /locus_tag="HV060_RS26505"
                     /old_locus_tag="HV060_26505"
     CDS             4488..5075
                     /locus_tag="HV060_RS26505"
                     /old_locus_tag="HV060_26505"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181213545.1"
                     /translation="MNKTYGYTRVSTREQTADGQRDAIAAHYPDALVLGEEPMSGTVPP
                     RERPTLGILLNQILQEGDTLVVAAIDRIGRNARDTLELVEEMEKRKINLISLREGVELN
                     TATGKSVIGVVASFAEMERTLILERQAAGIAAAKKRGQKFGRPTKIGATEVGEIVTRRA
                     MGVPIASIAKQLKLSRSAIYRCLEKIDAKAPE"
                     /name="recombinase family protein"
     gene            5305..5517
                     /locus_tag="HV060_RS26510"
                     /old_locus_tag="HV060_26510"
     CDS             5305..5517
                     /locus_tag="HV060_RS26510"
                     /old_locus_tag="HV060_26510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_147191448.1"
                     /translation="MTYRLSPRLKALARWTKLANNGARWALLNTRDEVVAWGRYLSDYT
                     LCSASRRTDVRLVNVAEEIDRLIEN"
                     /name="hypothetical protein"
     gene            5752..6219
                     /locus_tag="HV060_RS26515"
                     /old_locus_tag="HV060_26515"
     CDS             5752..6219
                     /locus_tag="HV060_RS26515"
                     /old_locus_tag="HV060_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_123923992.1"
                     /translation="MKKYTTEQKAQALKLLEQDGATSATVARTMGIPPRTVRDWAKARA
                     DAPSNVLSIEEMRERAQRAVEATPQAAVRRLKNHFVQRQYELLQRHASDLQALRSASLQ
                     AMLEKDATMVKAISGLMTSLLKAQERERLIYEIKPGTEADIMRQGMNRHQS"
                     /name="transposase"
     gene            complement(6906..8429)
                     /locus_tag="HV060_RS26520"
                     /old_locus_tag="HV060_26520"
     CDS             complement(6906..8429)
                     /locus_tag="HV060_RS26520"
                     /old_locus_tag="HV060_26520"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_061548411.1"
                     /translation="MPNHDDNSGAAVATVTQIAAAKAKAAAKTERPAFMGGSLPPVPFN
                     EWDGLTGSPTRDGFRIQAAEKRSVNFGNEYPLRYVPPLITDAGSGRWDVLNHADNLRWL
                     LYRNGARMELNLMTFKNELSWADSGSLVEPDEHGARAWILNKSAEVGLKRQAVDDFMFY
                     VARSAPYHPVARLLGSREWDGSPRVDAVLDCVPVAPGKEALRSALLRGLLIAAITAVEE
                     GAVSNKYVPCLYSEANNWFKTAFFRALFDLLPGAFGQGVSINPSSKDSVRPAVMRWFNE
                     LGEVSGMMKKNDPDEIKAFVERNVDEWRPENEKAVIAKKRQMVMGATVNKRDYLKDETA
                     ASRFPTITLVAPIDIARVNELLGWEWDNVKGCAVRADEDQLIQFWLEVRHLYRSGVGFN
                     LDDETFAAAKAETAQFVDRGSYYEPVRDILLLPGNASTGQYPWDKPGYDGRFTATDVTA
                     ALRAPSSHSGRVGRALKRLADEGIVDRRNHRGTFVYWKLPDEPTGELPL"
                     /name="VapE domain-containing protein"
     gene            8975..9454
                     /locus_tag="HV060_RS26525"
                     /old_locus_tag="HV060_26525"
     CDS             8975..9454
                     /locus_tag="HV060_RS26525"
                     /old_locus_tag="HV060_26525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548410.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKNARQFAQQTARQSARQIVPRGADPRFQAVSD
                     EADQLIVSMTGENATLPAIASALNAAGYRTATGQEWNTSRVRDYRHKLRESGLLC"
                     /name="hypothetical protein"
     gene            9524..10015
                     /locus_tag="HV060_RS26530"
                     /old_locus_tag="HV060_26530"
     CDS             9524..10015
                     /locus_tag="HV060_RS26530"
                     /old_locus_tag="HV060_26530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548409.1"
                     /translation="MAIGDDAIRDAFYIFTQQAAEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDLPTSPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAARRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCRAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(10383..10829)
                     /locus_tag="HV060_RS26535"
                     /old_locus_tag="HV060_26535"
     CDS             complement(10383..10829)
                     /locus_tag="HV060_RS26535"
                     /old_locus_tag="HV060_26535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548408.1"
                     /translation="MKSKTELRAAATRRALEVIASEMGGWSLDGFAHGSLPNFSPLPSQ
                     QTQEGSVVLARSPFECSWAGTAVFTAQASRFVNADPASAHYEWACGVEREAVATALMVE
                     AFNVTGCVEYAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAA"
                     /name="hypothetical protein"
     gene            complement(11086..12258)
                     /locus_tag="HV060_RS26540"
                     /old_locus_tag="HV060_26540"
     CDS             complement(11086..12258)
                     /locus_tag="HV060_RS26540"
                     /old_locus_tag="HV060_26540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172743605.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFDPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGSQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVEVMLVPDAGEAYRLAHKAAYGDDREPVPTI
                     PGASVVSFDGLNRFEPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERG"
                     /name="hypothetical protein"
     gene            complement(12245..12454)
                     /locus_tag="HV060_RS26545"
                     /old_locus_tag="HV060_26545"
     CDS             complement(12245..12454)
                     /locus_tag="HV060_RS26545"
                     /old_locus_tag="HV060_26545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
ORIGIN
        1 aagcgcgact gaaaaattcc ccgagcagct ccccggcgtt ttgttcgtca cctgccccgg
       61 cgggtgctcc ctccgggtcg aggcatgccg acagatttat gagcagagcc acctcgatcg
      121 cacgattgaa aacccccacc tcgagtgcca ccggtgcccc actggcctcg cgctttgggt
      181 cggtaccgaa cagccccctg acgcaccact gaccaccccg gcagacaccc gtaagctctg
      241 cccccgttgc agccggaaag acaaccgcct gatcgccccc aataagcccg gttcaagcct
      301 gtgcgtctcc tgctggaacc gcgaacgcga agccaccatc ggagccaacg cacgcgggag
      361 agtcaaccgc accccaccgt tcgtctcgcc gttcctgatc ggcatcggcg atggctggca
      421 ggtctggtac ggcatccacc agggcgaggc gctcgtccgc gccatgcgag ccaacccggg
      481 cgcacggttc catgatcgcc gaccaggccc ggtcgggcaa acacgccgcg acggtttcgt
      541 gtactactgc cccgaacacc gcgagcccct gcgctggaac ggtggcgacg ggcacgccgg
      601 agaggtggag cttgcttgtc ccaggtgcca gccatgggcg gcacccctca gcgtcgcccc
      661 cacgtttcag acggtcagct tccagacacc gcatgacgcc gcgcaccagt accggaacgc
      721 caccctgcca gacaacgcgg tctggacagc gtcggtctgc tccgattgca aagccgcgcc
      781 cctggtgatc agcaaggcac cggtcaaggg caagccgcga cgggagatcg tcgtctcctg
      841 cccgtcctgc gactccagcg catcagccag gcccggaggc aggacatgga cagacgagca
      901 catcgcggtg cccgagttcg cgcagaacga gacgcgcagc ctggcgaaga tccccgttat
      961 tttgccgcag agggagcagc cgcagctgct caaaagacca gggacagggc gcggtccagg
     1021 acgcccccgc aagcgcatcc ccctgaccct cgaccgggta ccagagagtc ggttcaaccg
     1081 cctcaccctt tccgtccttg ccgccgtcgg gcgctgggac tacatcaacg acatcatccg
     1141 gcgcatcgat cgggaccgcg gcgacgccgc caccctgatc gcccttgccg accacatcca
     1201 gaccaaccga ccagagagag atttatgacc gaaaccaccc gtccgatcct gaccatcaag
     1261 cgacgcaacc gcacggtcta cgtcccgccg gaaaaggcca cgaaatctga gccacagcca
     1321 gccgaacgga ctaccagcaa ggcgaaagta cagccaaagg tgaaaacccc gcagaagccc
     1381 gcacaggagc ccacagcggc aaaggcgaaa aagacgcctg aacaggtcgc cgaggggatc
     1441 cgcagacagg aggagaacga ccgccgacag caggacatag cgatcgccaa caagtccaaa
     1501 aaggcgctgg ccaaccgggt gaccaaagca ttcctgaaag agcactggcc tgacttgctg
     1561 acccggggag ttgaccgtcg ccccatggca aagggcatca tcgagcagat cctgacccga
     1621 ggcgcagagc tggacatgcc gccagaggca gaccgcaaag ccgtccgtcg catcctgggc
     1681 attgtcacgg gtaccagctc gtaccagcag tgcctcatag accagtgcat gcgctacgac
     1741 ctcgacggca accccaccgt cgccgtcacg ccagaggagg ccgaacaggc caaaacccgc
     1801 ctgacagaga tccgggaatt ccgacgcaag gcacgccagg agaaggacaa aaaaaagggc
     1861 gcataacgcg ccccgtctca ccgtttcacc gccctgtcga ccggggcagt tcccgtctca
     1921 cccagcgcca cgtaggcgca gcacgacgct ctgcagcacg gcgagcagct gcgacacgtt
     1981 gagcctgtcc ccttgcccgt cgggcagcaa tgaccccatc ggggatcacc acctcaggaa
     2041 tgaccaggcg cgagacagca aattcactca tcgccacccc cgaacgtata ggacaggttc
     2101 acaccgtttg cacgctgctc cagcgcctcg ccagccgcag caacatcagg atcagaagcg
     2161 accagctccc gggtgcgctc acgccggaac cgcagcccca gcgcatacag cgacgcatcc
     2221 gaacggacga aagccagcgt ctggccaccc gcagcgagcg agtctttaaa ccgggcaata
     2281 gcccgcttct cggtggcagg acgatcgccc ggatggtgat gcaggaacgg caacagccag
     2341 atcagctcca gccgttgcga gcgcgtgatc ctgctggaca gcggcatcaa cacctcgccc
     2401 tgcaggtgac gcggcagctt acgcattcca gtcaatacca cgcgcagccg gtgacagcgt
     2461 catgccgccc gcctgaacct gacggcgcac ctcggcaaac gccggagcac gcgccgcgtc
     2521 tggcatccca cgagaggacg tcaacacgcc gtcaagtaac accaggtcag ccggtgccac
     2581 acccgccgct ttcatctcgt tcaggaacgc cgcgtcctgg atggactgcg cacgcgcacg
     2641 gtgaaccgct tcggtcggag acgccaccgg agccccgatc ggcgcgaacg tctgcgctgc
     2701 gccgcctgtc tccaccactg tctcgaccac cgacgacagc ggagggaggc caaagtcatc
     2761 agcgccggtg gatttggtca gcgtcttgca acctttaacg tcgttcatgc gcttacggtg
     2821 gagagacgcg atcgcctcgt cctcctccag ctccccagcg ggctttttgc ccttcttgcc
     2881 gcccttcagg tcgggatcca gagattgctt tttgaaagcg ccggccccgt cagattttgc
     2941 cagcgtcgcc tcattgcgaa gccggtcaca caggagtttg aaagcagaca ttacacaccc
     3001 tccatgccac ggttacggcg caggatttca atagcgccct gcgtggcgaa aatttggtta
     3061 agacggcggt caggcgcgat cacggtacgg tacaggccgc gcccgccgtc ggtttcagcg
     3121 agcgtcgtcg cctcgtccat cgccagcttg agaagcagct cgatacgctg cacgcgaacg
     3181 gagtgcgccc agttgaacag cgccagcgca gccgccgcgt cacggcgatc ggcatcctgc
     3241 agcagggtgt cacgctcccg ggccacgtcg gcctcagcag cctcagcaac aaccagctcg
     3301 gcctcgatgt ccggcagcag gaacgacgca acacccgcgt cggggctcac cggctcgcca
     3361 gcacgcagcg cagccttgcc gtcggcgatc aaaccccgca gccgttcggc ctcggtcttg
     3421 agcccttcca ccactcgagc ggcgtccagc cgtcgcgctt ccacgtcgga agcggcctgt
     3481 cgcatttcac gcgcagcctg acgatcggcg agcgccgaga gctgaatgga ggagttcgtg
     3541 tctacctgca gggacgggag gtcatacaca gggaacgcat cacccgccaa ttcggcaagg
     3601 gtagagcgta gccaagcgcc gtcggcgctc tctttcttaa tttcggtcat ggttaggttc
     3661 tcagcaagtt atcagcaaag cggcaagaaa cgccgctcag acagcatgcc gtcacgacca
     3721 accgagcaca cccacgcaga gggcgcaggg ctctcgccca gcgcggggtg atcacgtcca
     3781 aaaaggagcg aaactgcgca gcccccgtgg ctctaggcgt ttacagtttt ttgcgcttgc
     3841 tttttgctca taagtggtca aatatccaca aacaaccttt tttgtacaac aggagacagc
     3901 atgagcagag tattcgggta cgggcgcgtc agcaccggag aacagacagt agccggacag
     3961 aagaaagcga tcctcgatcg ctacccacaa gccaccttcc acgccgacga gggcatcagc
     4021 ggaacggtac ccgccaccca gcgcgacaag ttcaaagcca tcctggagtt tgccgcacca
     4081 ggcgacagca tcgtggtggt cgcgatcgac cgcatcggac gcaacgccag atcggtcatg
     4141 gaggtctgcg aggagctgga gaagcggcag gtcagcctga tcagcctccg cgaaggtttc
     4201 gacttcagca ccccgaccgg gaagatgatg ctcaccatgg tcgctgcctt cgcagagatg
     4261 gaactcgcca ccctcaagga gcgcacaaag gcagggatgg cagcagccgc agccgcagga
     4321 cgccacgcag gacgaccgac caaacagacg ccggagaacg tcggagcggt gatcatgaag
     4381 cacgatgcag gagtaagcgc cgcacgcata gcacgcgacc taggcatcag caggggaacg
     4441 gtgaacagaa ttttacagaa gcacgcgaaa gcgtaagaga gcgaaaagtg aacaaaacat
     4501 acggctacac ccgtgtctca accagggaac agaccgccga cgggcagcgg gacgccatcg
     4561 ccgcgcacta ccccgacgcc ctcgtccttg gggaagaacc aatgagcggg acggtaccac
     4621 cacgcgagcg tccgaccctt ggcatcctcc ttaaccagat cctgcaggag ggcgacaccc
     4681 tggtagttgc ggcgatcgac cgcatcggca ggaacgcccg ggacaccctc gagctggtcg
     4741 aggagatgga gaagcggaag atcaacctca tcagcctacg cgagggggtg gagctaaaca
     4801 ccgcaacagg aaagtcggta ataggcgtgg tggccagctt cgcagaaatg gagagaacac
     4861 tcatcctcga acgccaggca gcagggatcg ccgcagcgaa aaagcgaggc cagaagttcg
     4921 gacgcccgac caagatcgga gccacggaag tcggcgaaat tgtgacgcga cgcgccatgg
     4981 gagtgccgat cgccagcata gccaagcagc taaaactaag ccggagcgca atttaccggt
     5041 gtcttgagaa gatcgacgcg aaagcgccgg agtaagagag agcgcagcgg taccgagtta
     5101 tggtgactcg aatggtaccg ccgcacccag gcccgggaag tgtgagagcc aaccgggcgc
     5161 gttaaaccac ccaatgaaag aggttaaccc catgatcgta tcgcacaaca gcgtcgcacg
     5221 ccagctggca cgccgcgcag taaccacccg caacccgcag acctggcgca gagccatgcg
     5281 ccgcctacgc cgtgaggtgg cagcatgacc taccgcctaa gcccacgcct caaggcgctg
     5341 gcacgctgga cgaaactggc caacaacggc gcacgatggg cgctcctcaa cacccgcgac
     5401 gaagtggtcg catggggtag gtacctcagc gattacaccc tttgcagcgc cagccgccgc
     5461 accgacgtca ggctggtcaa cgtcgccgag gagatcgacc gcctcattga aaattaagcg
     5521 ccgcttacgg tgtaggtgat tcttacttac accgtagcca aaccttacac ccggggcgtg
     5581 aaagcgcccc aatccaccag aagccctcag acgcgctgta agcccctcag ccacaaaacc
     5641 gcaccgacgt accaccaagc acgcgacagc gccgcacacc ccccaaaagt tgccgccagt
     5701 ttcagcgcgt gacgttccaa tggagaggaa cgaacaaccg gagcaggaac catgaagaaa
     5761 tacaccaccg aacagaaagc ccaggcactc aaactactgg agcaggacgg cgcaaccagc
     5821 gccaccgtcg cacgcaccat ggggatccca ccccgcaccg tcagagattg ggcgaaagcc
     5881 cgcgccgacg cccccagcaa cgtcctcagc atcgaagaga tgcgcgaacg cgcacagcga
     5941 gccgtcgagg ccaccccaca ggccgcagtc cgacgcctca aaaaccactt tgtgcagcgc
     6001 cagtacgagc tactccagcg ccacgcgagc gacctgcaag ccctacgcag cgcatcgcta
     6061 caggccatgc tggagaagga cgccaccatg gtcaaagcga tcagcggcct catgacctcc
     6121 ctgcttaaag cgcaggaacg cgagcgcctc atctacgaga tcaagccagg caccgaggcc
     6181 gacatcatgc ggcagggcat gaaccgccac cagagctgaa aacggcggga aagccgcctt
     6241 ttaccgccgt tttaccgccg cagaaaaccc gggagccccc ggaatacgcg gatccgcacg
     6301 ttacgcaatt gttaccgctc gcgtgcgcga gaagccccgt tttgatgccc ggacgaacca
     6361 aacgcgcagc tgcagctacg cattccactc ataacgcaga gggcgcgagg gcacgccctc
     6421 acgctgaacc accaagcgac cacccagcga agcgacagct tcgtcctctg cgcccccgat
     6481 caccccacct cacaaggcaa gcaaagtgca tccccttcag gggtgcagct gcggcacgcc
     6541 aactgcgcaa tttttgagcg tgtgggggta tgagggtgta ggggtaacat atgggcgtac
     6601 agggggtgtg ggggctactt attacccctc tttttacata gtagataaat aaaagaatgt
     6661 acccccacat accccaacac gaaagccaca tgcagcgcca tgaaagggat tttgagtatg
     6721 tggaggtaga ttttctttgc gagtttaccc ccacatctta ccccaacatc accagttacc
     6781 cccacacgtt acccccacac cgaaccccca catgatcacc ccataccccc acacaaaacg
     6841 gaacccccac acgggtaccc caacattggc agaggcataa aaaaacccgc cgaagcgggt
     6901 cattttcaga gcgggagttc cccggttggt tcgtctggca atttccagta aacaaaagta
     6961 ccccggtggt tcctgcgatc aacgatcccc tcatcagcta ggcgcttcaa cgccctaccg
     7021 acgcgaccag aatggcttga tggggcacgc agcgccgccg tcacgtccgt cgcggtaaac
     7081 ctgccgtcat agccaggctt atcccacggg tactggccag tggacgcatt gccaggcaac
     7141 aggaggatgt cgcgcaccgg ttcatagtac gagccgcgat cgacgaattg agccgtctca
     7201 gctttcgccg cagcaaaggt ctcgtcgtcc aggttgaaac cgacgccaga gcggtacagg
     7261 tgacgaacct cgagccagaa ctggatcagc tgatcctcat ccgcccgaac agcgcagcct
     7321 ttgacgttat cccactccca cccgagcagc tcgttaacgc gagcaatgtc gataggggcg
     7381 acgagggtaa tggtcgggaa gcgggacgcc gccgtctcgt ccttcaggta gtcgcgcttg
     7441 ttcaccgtgg cacccatcac catctggcgc tttttggcga tcaccgcctt ctcgttttca
     7501 ggacgccact catccacgtt gcgctccacg aacgccttga tttcatcggg gtcgtttttc
     7561 ttcatcatgc ccgacacttc acccagctcg ttaaaccagc gcatgaccgc agggcgcacg
     7621 ctgtccttac tcgaagggtt gatagacaca ccctgcccga acgcaccagg cagcaggtcg
     7681 aacagagcgc gaaagaacgc cgtcttaaac cagttgttag cctcggagta gaggcaagga
     7741 acgtacttgt tcgacacagc cccttcctca acagcggtga tcgccgcaat cagcagtcca
     7801 cgcagcagag ccgagcgcag cgcctcctta ccaggtgcga caggtacgca gtccagcacg
     7861 gcatcgacgc gaggagaacc gtcccactcg cgggaaccga ggaggcgggc aacagggtgg
     7921 tagggagcgg aacgggcaac gtagaacatg aaatcgtcaa cagcctgacg tttcaggcca
     7981 acctcggcgc ttttgttcag tatccaggcg cgggcaccat gctcgtccgg ttcgaccaga
     8041 gagccgctat cagcccatga gagctcattc ttgaaggtca tgaggttaag ctccatccgg
     8101 gcaccattgc ggtagagcaa ccagcggaga ttgtcggcgt gattgaggac gtcccagcgc
     8161 ccagagccag catcagtgat cagcggcggg acataccgca gtggatactc attgccgaaa
     8221 tttaccgatc gcttttcagc cgcctgaatg cggaaaccgt cgcgggtcgg gctaccggtg
     8281 agcccatccc actcattgaa cgggacagga ggcagagaac cacccatgaa cgcagggcgc
     8341 tcggtcttgg cggcagcttt ggccttggcg gcggcgatct gcgttacagt ggctacagca
     8401 gcgccagaat tatcatcatg attaggcaat tttacatcct caaaattgac atcgaatctt
     8461 gaggagccct acaacggcgg gactttacgg ggaaaacacc ctttgcacgc gctggaaagt
     8521 tcgcgtacaa ttcaagctgg attgccaccc aacctgttgt tagaatcggt gtcttccacg
     8581 gttcccgccg cgaaagctcc ttaaagtttt aacaacaaca gctttaaaga aaatgacccg
     8641 tctgccagga cgggttttct ttttcagccg cgtcatcata ccgttacatt cccccctgta
     8701 ccagcacaca acgatccaac cacgcgaaac gcgatccagc tccagcctta cagccgcgta
     8761 gcctctacca cttatccacg ggccagctcc gccgcaaaat acttgtccac agaaactgtc
     8821 cacaggcacc ccggcgacgc aacgccgtcg cccctcagcg cacgacaatt gcactacgtc
     8881 aaattcgacg cgcacgacaa aacgcattac actccgcaag acaaacagca cgacaaatag
     8941 cacgacaaat agcacgacac ggagatgcta gcccatgcga gagaaaacga aaaccctgac
     9001 cgttcgactg cccgtcagcc tgatcgagcg catcgagcag cgagcggaga aaaccaaagt
     9061 aacccgcaca gatgccgtca tagaggcgct cgtcgccgga ctcggcacga agcctgaagg
     9121 gatggacacc aaacttttga ctgccatcga ggcgctgcgg tcagaggtca gggcgctggc
     9181 cggaggcggg aaaaatgcac gacagtttgc acaacaaacc gcacgacaaa gcgcacgaca
     9241 gatagtcccc cggggagccg atccacgctt ccaggccgtc agcgatgagg ccgaccagct
     9301 gatcgtcagc atgaccgggg agaacgccac cctgcccgcc atcgcgtcgg cgctgaacgc
     9361 agccgggtac cggacagcga cggggcagga gtggaacacc agccgcgtcc gggactaccg
     9421 ccataagctg cgggagagtg gtctattgtg ctgagttagc agtcctaagt gaggtaatta
     9481 aatatgataa aatgcagcct taaattattt ataaggaaag tcagtggcta tcggagatga
     9541 tgcaatccgg gatgctttct acatcttcac gcaacaagct gcggaaatgg atgataaggg
     9601 gctccctcaa gaagtctggg agactccttg tttcacttat ttgatgacta aaaaacaatt
     9661 caatcagatg aaggttgttt gtcagagaaa cggctgggat ttgcctacgt cgccagcaat
     9721 accaattact tgggctatgt ttaggcatgt gctatctgca cgtaactcga aggataaatt
     9781 aagttggcag gagtgtgcgg aaattttggc tactgcattt tcagtgcaga gtaacgtcta
     9841 cgttaacagg gattattctg agcagactat tgtgctgaat gctgctcgtc gtattaacgt
     9901 cgctggggct ggttttttcg caatggcgat agttgatgtc tctgaaaaca atttggcccc
     9961 cgtaactgca taccatgcaa cggaggccaa gtgtagagct atacagcgag ggtgatagga
    10021 aggacgccca tttaagcagt tctcctcaat ttaatgagaa tgaaccataa cgcctaaata
    10081 tttcccttca ttggttcctt tcggatctag cagcatcggc ggttttctgc tgtctatcaa
    10141 attcgctatt ggtcaggagt tttagctcct tactgctgac tacagaataa tttacgtatc
    10201 tgtagaacgc tcttgcagta aaataattct atacggatta cagctaagat tcaaccaaaa
    10261 gtggaatgat aatgctctcc tgaactcaat gagcccgcgt cacctgtggc tttagccgta
    10321 gccatctagc gtatccagcc tctgcgtcag cgtggcctga tctgacgcag aggagcgacg
    10381 gcctacgccg ccgcatctaa ccaaggcagc atcgccgccc tgatcagctc cacgtcggcc
    10441 tcgtccatgg tcagcagcac catgccgtcc acaggaggca acccggcgta ctccacgcag
    10501 ccggtcacgt tgaacgcttc aaccatcagc gcggtggcca cagcctccct ttcgacgccg
    10561 cacgcccact cgtagtgagc agacgccgga tcggcattca cgaaccggct cgcctgagcg
    10621 gtgaacacag cagtgccagc ccacgaacac tcgaagggcg atcgagcgag caccaccgag
    10681 ccctcctgcg tctgctggct aggcagcggg gagaagttcg gcagcgatcc atgagcaaac
    10741 ccgtcgagcg accagccccc catctcagaa gcgatcacct caagggcgcg acgggtcgcg
    10801 gcagcgcgta attcagtctt tgatttcatg tgccctcctt acgggcgaaa aaaagcccga
    10861 caccaaaacg gtgccgggct tgagtgtgct gctggaaacc aaaccagctg caggatagcg
    10921 tcacgaccgc agcggcgtct aaaacggagg aaaccggaag cggtcggatg tgcgtcttaa
    10981 aggttgtctc accaacccaa aagcgactgc aaaaaagacg agccagaaaa cgacccttta
    11041 cggtcagcgg cctcccgagg aaaccgacgc aaaaaagccc agccatcagc cccgctcaag
    11101 ccgggcatga cgcaccgcat accggagcag ctcacgcgcc cgatcctcgc cctcctcaac
    11161 cagcacgtcg aaccagtcga ggttatcgcg gggctcgaag cggttcaggc cgtcgaacga
    11221 caccaccgac gcccctggaa ttgtcgggac cggctcgcga tcgtctccat aggccgcttt
    11281 atgcgccagc cggtacgcct cgcccgcgtc cggtaccagc atcacttcaa catcaggcca
    11341 gaaccgacgc agcgacgcca cgcagctgcc gaacccggcg atcgagaacg cagcgcagac
    11401 cgccaccttg ccacggctca ggtggtgcag ggcgaacgcc gtagcgaacc cctcacagac
    11461 gaacagaggc gagtagggcg cgtgcgggga gccgtcgagc acgtccatga accccggcat
    11521 cgggtagaag ccgccctgct tgcgggtacc cgccggagag aacttcgagc cagtgagctg
    11581 ggaaccctca gccgagcagc gcccgcagat ggcctccagg ccgtcaacat acagctgacg
    11641 ggcggcgtcg aacttgtaaa ccggaatcag gaggcaatca gaccagtaag cacgcggatc
    11701 gaacgatcga aacatcccgc caacaccaag atctgtgctg agggcgggca gctggacgcc
    11761 acccgcagcc gccagagcgg tctttttgcg ccagtatggg gtagacgcag gatcccctcc
    11821 cttcgccagc agtttgaccg cagcacgcgc agcagcatca cccttcttgt cgcgttcagc
    11881 ctttgccttc gctgtggcct cacgggacaa ccgcagccgt tcagcgcgat cgacgtcgga
    11941 ctggacagaa gcgcgaccgt tgcgccattc gacgccggag aaattgagga aagcctgagc
    12001 ggcgaatgta tccaggtcgc cttttaaaaa cgacctgtac tgctggcgat ggcgattctc
    12061 gcctcgccca tgtcgagtgg accaggcctc gatagctgcc agcccgtcct cgccgcagag
    12121 agacgcccag caaccaagag ccaggcagag acgcacccat tcgtctcgat cagccggagc
    12181 acccgcagcc gatacggcac gccagaacag gcccggatcc tctttcagat gggtagtgtc
    12241 gaaatcaacg aacgccatca gcagccctcc ccggacgacg ctggtcggca acgaagcgtt
    12301 tgatctcggg attgccagcc agccagtaat gcccgccacg gagggagcgc cctgccggag
    12361 tcggcacgat cccccgcgaa cttagccgcg agagcgtgcg cagcgacacg ttcagcagcg
    12421 cagcgaggtc agcggtatag attcgttttt tcattgggag taccagatag gtgcccccct
    12481 cagcttaacg ggcatcgggg ggcgggagtg acgattatcg ataaagccgc ccttgaggac
    12541 ttaccgcata gcggcaggac agcagccctt ctcgcttact cgcacctgcc aaggacgaag
    12601 cgcgcatcct cgcttacaac cccgcctcgt tggggaggag ggaaagcggc accgggcgat
    12661 ccggtttcat gtttcccaac tgctcgtcag gaatcagtaa gacaagcgta acacaattaa
    12721 cgccaaagtg gcgttttttg tgagatacta aaaaagacgc atagccagtg ctggcgctgt
    12781 ctggcgacca tttcgacgca gggggagcag tgaacgacga taacgacacg aacgaatcga
    12841 cgcagccgaa cccggcagca cttgaacgcg t
//
