LOCUS       NZ_CP055587             7945 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00110 plasmid pRHBSTW-00110_6,
            complete sequence.
ACCESSION   NZ_CP055587
VERSION     NZ_CP055587.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148491
            Assembly: GCF_013589875.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00110
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.0804402021492x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:01:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,389
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,130
            CDSs (with protein)               :: 5,130
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 44 of 135
            Pseudo Genes (incomplete)         :: 90 of 135
            Pseudo Genes (internal stop)      :: 28 of 135
            Pseudo Genes (multiple problems)  :: 24 of 135
            Pseudo Genes (short protein)      :: 1 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055587.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00110"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00110_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6571..7945,1..734)
                     /locus_tag="HV074_RS26820"
                     /old_locus_tag="HV074_26845"
     CDS             join(6571..7945,1..734)
                     /locus_tag="HV074_RS26820"
                     /old_locus_tag="HV074_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_139690572.1"
                     /translation="MAIFHLTAKTGSRGGGQSAKAKADYIQREGKYARDKDEVLHTQSG
                     NMPSWAAGAAEVSNYWDAADLYERANGRLFKEVEFALPIELTLDQQRVLVDDFAKYLTE
                     VERLPYTLAIHKGGDENPHCHLMMSERKNDGVERKKDQWFSRYNPKKPGQGGAQKTESL
                     KPKAWLEKTRAAWADHANRALEMAGHEARIDHRTLEAQGIERLPTIHLGPNVVEMEGRG
                     IRTGRADTALSIETANTEIIDLQAYRESIDHERDRQSEAGQRDRRAGGADRAAGAELGD
                     SRRQGRDGDRRDSKCKHKAGWRVEDPAGQSREGVGRHSQGHEGRSRGTGHGSREGRGRS
                     ESVGMEDFRSHLDGFSAVYASAADRIVALAVPGTQDDKGGGSVATTQAQVKGDRTSQAI
                     ARQLKAMGCDLYDIGIRDSATGKMMNREWTLQEVVENAAWLKRMNAQGNDIYIRPAESA
                     RHGLVLVDDLSSFDLDAMKEEGREPAAVIETSPKNYQAWVKVAANAPAEHRGAIARRLA
                     REYDADPASADSHHYGRLAGFTNQKPKHTTKTGYQPWVLCRASSGQAATTGPQMVQEAG
                     QELESKERQQEQARRLEGIETAQPRRYHRTALDEYRSEMAGLVKRFGDDLSKCDYIAAM
                     KLAMKGIDPDEIAKAMAEASPGLMDRKAGHEADYIERTIKKVMALPEVQKIRAELEQTQ
                     KPSGYTPR"
                     /name="DNA-primase RepB domain-containing protein"
     gene            819..1025
                     /gene="urfF"
                     /locus_tag="HV074_RS26825"
                     /old_locus_tag="HV074_26850"
     CDS             819..1025
                     /gene="urfF"
                     /locus_tag="HV074_RS26825"
                     /old_locus_tag="HV074_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_139690571.1"
                     /translation="MKDKRDKLTGNLLASDNAIRQARFAETQRELGRKARKIWATDDEA
                     ETLRLYLDKIRAAQDGDRDPASA"
                     /name="urfF"
     gene            1056..1898
                     /locus_tag="HV074_RS26830"
                     /old_locus_tag="HV074_26855"
     CDS             1056..1898
                     /locus_tag="HV074_RS26830"
                     /old_locus_tag="HV074_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_139690570.1"
                     /translation="MATHNPIDILAAFTAPPPPIDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAMQIAGGPDLLGVGELPTGRVIYLPAEDPPVAIHHRLHALGAHLSAAERQAVAT
                     DLWIKPLIGNGPNIMTPEWFDGMMKAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAAETGCSIIFLHHANKSAAMAGAGDQQQASRGSSVLVDNIRWQAYLSGMTAAEAET
                     WGVDDNQRAYFVRFGVSKANYGSPFQERWLRRHEGGVLKPVPLERQAAKAKGTQRDKI"
                     /name="helicase RepA family protein"
     gene            1885..2721
                     /gene="repC"
                     /locus_tag="HV074_RS26835"
                     /old_locus_tag="HV074_26860"
     CDS             1885..2721
                     /gene="repC"
                     /locus_tag="HV074_RS26835"
                     /old_locus_tag="HV074_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_139690569.1"
                     /translation="MTKYNLTHLRHDPSHCLAPGLFRALKRGERKKLKLDVAYDYGDGK
                     RVEFSGPEPLGADDLRVLQGLIAMAGPSGLVLGPDPKTEGGRQLRLFLEPKWEAVEADA
                     LVVKGSYRMLASEIGAGEGGEQFRIIRDVIERLWKVSIIAQNGRKRQGFRLLADYASDE
                     ADGRLFVALNPLIAQAVMGGGQHVRISMAEVRALRSDTARLLHQRLCGWIDPGKSGKAT
                     VDTLSSYVWPEEASGATMRQRRKRVRDSLPEIVSLGWTVTEYAPSKYNIARPKATA"
                     /name="repC"
     gene            complement(3681..4058)
                     /locus_tag="HV074_RS26840"
                     /old_locus_tag="HV074_26865"
     CDS             complement(3681..4058)
                     /locus_tag="HV074_RS26840"
                     /old_locus_tag="HV074_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_008786737.1"
                     /translation="MTSRKTTSADTAQCSTTSHDAATDALAMSQSDVAVLAETFRLLGD
                     QSRLKILLQCMHGSVAVGDIAEALDLSQSLVSHHLRLLRGARLVRGERQAKHIFYSIAD
                     QHVSQVLQDMAVHISEDKADD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(4102..5199)
                     /locus_tag="HV074_RS26845"
                     /old_locus_tag="HV074_26870"
     CDS             complement(4102..5199)
                     /locus_tag="HV074_RS26845"
                     /old_locus_tag="HV074_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_008786738.1"
                     /translation="MSQSHAHDHGHDHDHDHDHTPTVTSANERKVLLSFFLIFGFMVVE
                     AVGGVLSGSLALLADAGHMLTDAAALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLN
                     AVTLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLVNVLVLWIMTRGETDHVNVK
                     GAILHVMGDLLGSVGAIVAAIVIYLTGWTPIDPILSVLVAALILRSAWKLLAKSIHILL
                     EGAPEDASPEKVEQGLMGAVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKR
                     VEAVLREQFSIEHATVGIDWNSGTDENVCSLQPTSERQDHSGHDHSGHDHSGHDHSGHD
                     HGNHKHGGGHSHGHDHDHSAPGHRH"
                     /name="cation diffusion facilitator family transporter"
     gene            5424..5681
                     /locus_tag="HV074_RS26850"
                     /old_locus_tag="HV074_26875"
     CDS             5424..5681
                     /locus_tag="HV074_RS26850"
                     /old_locus_tag="HV074_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="WP_019396551.1"
                     /translation="MEDMHKHTTHPDLVKRLKRAEGHLRHVIGMIEGGETCLDIARQLA
                     AVESAVTAAKRVLIHDHIDHCLSHDEDSVLTEMKALTKLL"
                     /name="metal-sensing transcriptional repressor"
     gene            5691..>5855
                     /locus_tag="HV074_RS26855"
                     /old_locus_tag="HV074_26880"
                     /pseudo=""
     CDS             5691..>5855
                     /locus_tag="HV074_RS26855"
                     /old_locus_tag="HV074_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /name="MFS transporter"
     gene            complement(6057..6245)
                     /locus_tag="HV074_RS27165"
     CDS             complement(6057..6245)
                     /locus_tag="HV074_RS27165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259472377.1"
                     /translation="MIEAGKLGQFNEVWLRAELDKQLDRDPDRALFGLPPLAKAKPEQV
                     VQEEAPAGLHTAPTAAQ"
                     /name="hypothetical protein"
     Typing          1057..1854
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          49..728
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.238"
                     /coverage="97.0"
                     /evalue="1.25e-138"
     Typing          6572..7944
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.598"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 ggcacgggct tgtcctggta gacgacttga gcagctttga cctggacgcg atgaaggagg
       61 aagggcggga gcctgccgcc gttatcgaga ccagcccgaa gaactatcag gcgtgggtga
      121 aggtagcggc aaacgcgcca gcagagcaca ggggcgcgat tgcccgcagg ctggcccgag
      181 agtatgacgc cgaccccgca agcgctgata gccaccacta tggccgtctg gcgggcttta
      241 ccaaccagaa gcccaagcac acaaccaaga cggggtatca gccgtgggtg ctgtgccgag
      301 cgtccagcgg ccaagccgcc accacaggcc cccagatggt gcaggaggcc gggcaggagc
      361 ttgagagcaa ggagcgccag caggagcagg cccgcaggct ggagggcata gagacagccc
      421 agccgcgccg ttatcacagg actgccttgg acgagtaccg cagcgaaatg gcggggctgg
      481 tgaagcggtt cggggacgac ctgagcaagt gcgattacat cgccgccatg aagttggcca
      541 tgaagggcat agatcccgac gagatcgcca aggccatggc cgaggccagc cccggcctca
      601 tggatcgcaa ggcaggccat gaggccgact acatcgagcg caccatcaaa aaagtgatgg
      661 cgctgccgga ggtgcagaag atccgcgccg agcttgagca gacgcaaaaa ccaagcgggt
      721 ataccccacg ctgaaaatgt gacttttagt tagagctaac ccatgttatg ctgttagctc
      781 taaccgtgta ttttgcatta gctctaatga ggtctggtat gaaggacaag cgggataaat
      841 tgactggcaa cctgttggcc agcgataacg cgatcaggca ggctcggttt gccgagaccc
      901 agcgtgaact ggggcgcaag gcgcgtaaga tatgggccac agacgatgag gccgagacct
      961 tgaggcttta tctggacaag atcagagcgg cgcaggacgg ggatcgcgac cccgccagcg
     1021 cctaaccaca gaccgcctaa acaggaggca aatcaatggc tacccacaat cctatcgaca
     1081 tactggcggc tttcacagcc ccaccccctc caattgatta tgtgttgccc aacatggtgg
     1141 ccggcaccgt gggggcgctg gtatcccccg gtggggctgg caagtccatg ctggccctgc
     1201 aactggcgat gcagatcgct ggcggccccg atctgctggg cgtgggcgag ctgccgacag
     1261 gccgagtgat ctacctgccc gcagaagacc caccagttgc catccatcac cgcctgcacg
     1321 cactcggggc acacctctcc gcagccgaac ggcaggccgt ggcaaccgac ttgtggatca
     1381 aaccgctgat cggaaacggc cccaacatca tgaccccgga gtggttcgac ggcatgatga
     1441 aagccgccga gggcagacgc ctgatggtgc tggacacgct caggcgcttc cacatcgagg
     1501 aggagaacgc ctccgggcct atggcccaag tcatcggccg catggaggcc atcgccgccg
     1561 agacagggtg ctcgatcatc ttcctgcacc atgccaacaa aagcgcggcc atggcgggcg
     1621 caggcgacca gcagcaggcc agccgtggat cgtcggtgct ggtcgataac atccggtggc
     1681 aggcatacct ctccggtatg accgcagccg aggccgaaac gtggggcgtg gacgacaacc
     1741 agagggccta tttcgtgcgc tttggcgtga gcaaggccaa ctacggcagc ccgtttcaag
     1801 agcggtggct aaggcggcac gagggcggcg tactcaagcc cgtaccgctg gaaaggcaag
     1861 cagctaaggc caaggggacg caacgtgaca aaatataact tgacgcacct ccggcatgac
     1921 ccgtcgcact gtctggcccc cggcctgttc cgtgccctca agcggggcga gcgtaagaag
     1981 ctcaagctcg atgtggccta tgactacggg gatggcaagc gggtagaatt tagcggcccg
     2041 gagccactgg gcgcagacga ccttcgcgtc ctgcaaggtc tgatagctat ggcagggccg
     2101 agcggtctgg tgcttggccc agaccccaag accgaaggcg ggcgacagct ccgcctgttc
     2161 ctagagccga aatgggaggc cgttgaagct gacgcactgg tagtcaaagg aagctataga
     2221 atgctcgcca gtgaaatcgg tgcgggagaa ggtggcgagc agttcaggat catcagggac
     2281 gtgatagagc gactgtggaa agtctcgatc attgctcaga atggacgcaa acggcagggc
     2341 ttcaggctgc tggcagacta cgccagcgac gaagcagacg ggcggctctt cgtggccctg
     2401 aaccccttga ttgctcaggc cgtcatgggg ggcggtcaac atgttcgcat cagcatggcc
     2461 gaagtccggg cgttgcggag cgataccgct cgcctcttgc accaacggct atgcggctgg
     2521 atcgaccccg gcaagagcgg caaggccact gtggatacct tgtccagcta cgtttggccc
     2581 gaggaagcca gcggggcaac catgcgccaa aggcgcaaaa gggtacggga ttcgctgcca
     2641 gagattgtct cattgggctg gacggtcacc gaatatgccc ctagcaagta caacatcgca
     2701 cggcccaagg cgaccgcatg acccctgtag taaccgtcac gcacccctgt agtaaccgtc
     2761 acgcacccct gtagtaaccg tcacgcaccc ctgtagtaac cgtcacgctc aggggagtaa
     2821 aaacaacgct gtaagccacg ccaccactgg cctgtagcat ttgcgcaaaa agttcatcca
     2881 agatcttcca agatcttcca taggatctga gccctttagg ggctccgacc ctctacagat
     2941 tacagaccta cagaccaatg cccggccaga ggccggaggc cctttatcgc tgcgctaagc
     3001 tacacacccc cccccactgc tgcgcagtag ggggaaaggc gggcttcgcc cgctggtgcc
     3061 tgtctgagac gccacaggag gcccgtagag gggttctagg gattttccgt ccaaaaacga
     3121 caaagtgctc tggaggccac gccgtccgcg gcctccagag gggtattact tttttggcgc
     3181 ttcacatagc cagtcacaat aaatcaatca cttagcagtc tgaatgtcgc atttggacgg
     3241 aaaatcccta gaaccccgaa gtgccccttt cttggatgca ggactccggc ccttccgaag
     3301 cgctctgcag aaatttgtgt ggaggctagg cgttacaacg aaggcaaggt caagcccttt
     3361 gggttgccct agcgggcagg ctgcatggac aaggtggtgg gaaaaccccg cgtcgtcgct
     3421 gtggccactc cgacatccag ctcacgagtg ccgttgtgcg gttgaaccgg tcggtcttgt
     3481 gggtgattgc gagcgcggga ggctcaggcg tccaagctgc cgccgcccat tgctgtgcct
     3541 gcgcggcatc ccggcgccca ggcagggccc gcgattcagg gtgttgcgcc caggccgcat
     3601 cgaggacgtc cttgaggcta accggcggcg tgggtaccgg cgcctcgcaa cctgccattc
     3661 gcctgggtgc ttgggcctgg tcagtcgtcg gctttgtcct cggaaatgtg gaccgccatg
     3721 tcctgcagca cctggctgac gtgctggtcc gcgatgctgt agaagatgtg cttggcttgg
     3781 cgttcgccgc gcacgaggcg cgcaccgcgc agcagccgca ggtgatggct gaccagagac
     3841 tgcgacaggt cgagcgcttc ggcgatgtcc cccacggcga ccgagccatg catgcactgc
     3901 agcaggatct tcaatcgcga ttggtcgccc agcaggcgga aggtctcggc cagaacagcg
     3961 acgtcacttt gcgacattgc gagggcatcg gtcgccgcgt cgtggctggt ggtcgagcat
     4021 tgcgcggtgt cggcgctggt ggttttgcgg gacgtcatgg atgcaaatcc tcaagtggag
     4081 tgcgagccgg ctcgcgcggc ctcaatgcct gtgaccagga gcggaatggt cgtggtcatg
     4141 gccgtggctg tgcccgccgc catgcttgtg attgccatga tcatggccgc tgtggtcgtg
     4201 cccgctgtgg tcatgaccgc tgtgatcgtg gccgctgtgg tcctgacgct ccgacgtggg
     4261 ctgcaagctg cagacgttct cgtcggtgcc cgagttccag tcgatcccga ccgtggcgtg
     4321 ttcgatgctg aattgctctc gaagcaccgc ttcgacgcgc ttgaccacgg cgcgtgcttc
     4381 ctcgtccacg ttggggcgaa cgtgcagcgt ggccatcgtg cgcccggagg tgagctgcca
     4441 cacgtgaacg tggctgacgg ccgccaggcc cggcacggcg cccatcaggc cttgctccac
     4501 cttctccggc gaagcatcct ccggcgcgcc ttccagcagg atgtggatcg acttggcgag
     4561 cagcttccat gcgctgcgca ggatcagcgc cgcgaccagg accgacagga tgggatcgat
     4621 cggcgtccag cccgtgaggt agatgacgat cgcagcgacg atggcgccga ccgagccgag
     4681 caggtcgccc atcacgtgca ggatggcccc cttcacgttg acgtgatcgg tctctccgcg
     4741 ggtcatgatc cacagcacga gcacgttcac cagcaggccg agcacggcga cgatcatcat
     4801 cgggccggcc aggatcacgg gcggcgcctg cagccgctcc cacgcctcgt aggcgatcca
     4861 agcgacgatc gcgaacaggg tcacggcgtt caggaaaccg gcgatgacct cgaagcgcag
     4921 gtagccgaag gtgcgcttgc tgtcggccgc gcggcggccg aaccggaatg ctgcgtaggc
     4981 cagtgcgagc gccgcggcgt cggtcagcat gtggcccgcg tcggccagca aggccagcga
     5041 gcccgacagc acgcctccga cggcctccac gaccatgaag ccgaagatca ggaaaaagga
     5101 cagcaggacc ttgcgctcgt tggcgctggt cacggtcggg gtgtggtcat gatcatggtc
     5161 gtgatcgtga ccatggtcat gtgcgtggga ttgggacatg gggcctcggg aaactgggtc
     5221 tttgactgtg gctcaaaata taacacatga acatgttctc atgtgaattg ttgtacacat
     5281 ttgtgttcgc gtctcaccac ggccgggatg ccgctccggg caattgcctt agcctggctc
     5341 ggcgaccatt ccccccgcct ggcaagcagc cgttgatcta acatccagtg gcagtatccc
     5401 ccctgggggg atatgatttc agcatggaag acatgcataa gcacaccacc catcccgacc
     5461 tggtgaagcg gctcaagcgc gccgaggggc atctgcggca cgtcatcggg atgatcgaag
     5521 ggggagaaac ctgcctcgac atcgcccgcc agctcgcggc ggtggaaagc gcggtgacgg
     5581 cggccaagcg cgtcctgatc cacgaccaca tcgaccactg cctgtcccat gacgaggact
     5641 ccgttctgac cgaaatgaag gcgctgacca aactgctctg aggccgtccg atgctctccg
     5701 tcctgaataa ccgcacctac cgccacctgt tcaccgccca ggtgatcgcg ctcgtcggca
     5761 ccggcctgat gacggtggcg ctcggcctgc tggcctacga gctggccggc gcggatgcag
     5821 gcgcggtgct cgggacggcg ctggccatca agatggggtt ctagggattt tccgtccaaa
     5881 aacgacaaag tgctctggag gccacgccgt ccgcggcctc cagaggggta ttactttttt
     5941 ggcgcttcac atagccagtc acaataaatc aatcacttag cagtctgaat gtcgcatttg
     6001 gacggaaaat ccctagaacc ccgtagagct gttttgccgc gacagcggcc tttcccctac
     6061 tgcgcagcag tgggggcggt gtgtagcccc gcaggggcct cttcctgcac cacctgctct
     6121 ggcttagcct ttgccagtgg tggcagacca aacagggctc ggtctggatc acggtcaagc
     6181 tgcttgtcca gctcagcccg tagccagacc tcattgaact gccccagctt tcccgcctct
     6241 atcatgcccg tgagcattgc cccaactatg atcttgcgcc gagtatcatc ccgccgttcc
     6301 tgctgctgtt cccttgccct cactcgctgg atctcggcat tgatccgcgc cctctgttct
     6361 tcgagcttgg cgagtttatc ctctaacttt cccatgttga cagccctgtt gttatgtgcc
     6421 cgttgtagag tatcatcagg ttactgatgc cgcaaacccg actcatggaa catgaagagg
     6481 gcgcacttac cggtttctct gcgagaaact gccttcggcc ccccttcggg gcggtgcgct
     6541 ctccgagggc cactagcgtg gagcttacac atggcgatat ttcatctcac ggccaagact
     6601 ggaagccgtg gcggcggcca gtcggctaag gccaaggccg actacatcca gcgcgagggc
     6661 aagtatgccc gcgacaaaga cgaggtgctg cacacccagt ctggcaacat gccctcatgg
     6721 gcggcaggag ccgcagaggt cagcaactac tgggatgccg ccgacctgta cgaacgggcc
     6781 aatggccgcc tattcaaaga ggttgaattt gccctgccaa tcgagctgac gctcgatcag
     6841 cagcgggtgc tcgtggacga cttcgccaag tacctgaccg aggtcgagcg cctgccgtat
     6901 acgctggcga ttcataaggg gggggatgag aacccgcatt gccacctgat gatgtccgag
     6961 cgcaaaaacg atggcgtcga gcgcaagaaa gatcagtggt tcagcaggta caacccgaaa
     7021 aagccggggc aggggggggc gcagaaaacc gagagtttga agcccaaggc gtggcttgag
     7081 aagacccgag cagcatgggc agaccatgcc aaccgggcgc tggagatggc aggccacgaa
     7141 gcccgcatcg accaccggac gctagaggcg caggggatcg agcgcctgcc gaccatccac
     7201 cttggcccca acgtggtgga gatggagggg cggggcatcc gcaccgggcg ggcggatacg
     7261 gccctgtcca tcgagacggc caacaccgag atcatcgacc tacaggcata cagagagagc
     7321 atcgaccatg agcgcgatag acaaagtgag gcaggccaga gggatcgacg agctggcggg
     7381 gcagatcgag ccgctggcgc agagcttggc gactctcgca gacaaggccg tgacggcgat
     7441 agaagagact caaaatgcaa gcataaagca ggctggagag tggaagatcc agcagggcaa
     7501 agccgcgaag gcgtgggaag gcacagccaa ggacatgaag gacgcagccg aggcactgga
     7561 cacggcagcc gggaaggccg agggcgcagc gagtcagttg ggatggagga ttttcgtagt
     7621 cacctcgatg ggttttctgc tgtttatgct agtgctgctg atcggattgt ggctctggct
     7681 gtccccggaa ctcaggacga caaaggaggg ggcagtgtgg ctactactca ggctcaagtg
     7741 aagggggatc gcacctctca ggccatcgcc cgccagctca aggctatggg ctgcgacctg
     7801 tacgatattg gcatcaggga ctccgccaca ggcaagatga tgaatcggga atggacgctg
     7861 caagaggtgg tcgagaacgc cgcatggctc aagcggatga acgctcaggg caacgatatc
     7921 tatatccggc cagccgaatc agcca
//
