LOCUS       NZ_CP055586            12351 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00110 plasmid pRHBSTW-00110_5,
            complete sequence.
ACCESSION   NZ_CP055586
VERSION     NZ_CP055586.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148491
            Assembly: GCF_013589875.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00110
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97.0804402021492x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:01:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,389
            CDSs (total)                      :: 5,265
            Genes (coding)                    :: 5,130
            CDSs (with protein)               :: 5,130
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 44 of 135
            Pseudo Genes (incomplete)         :: 90 of 135
            Pseudo Genes (internal stop)      :: 28 of 135
            Pseudo Genes (multiple problems)  :: 24 of 135
            Pseudo Genes (short protein)      :: 1 of 135
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055586.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00110"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00110_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(12107..12351,1..235))
                     /locus_tag="HV074_RS26745"
                     /old_locus_tag="HV074_26770"
     CDS             complement(join(12107..12351,1..235))
                     /locus_tag="HV074_RS26745"
                     /old_locus_tag="HV074_26770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548410.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKNARQFAQQTARQSARQIVPRGADPRFQAVSD
                     EADQLIVSMTGENATLPAIASALNAAGYRTATGQEWNTSRVRDYRHKLRESGLLC"
                     /name="hypothetical protein"
     gene            781..2304
                     /locus_tag="HV074_RS26750"
                     /old_locus_tag="HV074_26775"
     CDS             781..2304
                     /locus_tag="HV074_RS26750"
                     /old_locus_tag="HV074_26775"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_061548411.1"
                     /translation="MPNHDDNSGAAVATVTQIAAAKAKAAAKTERPAFMGGSLPPVPFN
                     EWDGLTGSPTRDGFRIQAAEKRSVNFGNEYPLRYVPPLITDAGSGRWDVLNHADNLRWL
                     LYRNGARMELNLMTFKNELSWADSGSLVEPDEHGARAWILNKSAEVGLKRQAVDDFMFY
                     VARSAPYHPVARLLGSREWDGSPRVDAVLDCVPVAPGKEALRSALLRGLLIAAITAVEE
                     GAVSNKYVPCLYSEANNWFKTAFFRALFDLLPGAFGQGVSINPSSKDSVRPAVMRWFNE
                     LGEVSGMMKKNDPDEIKAFVERNVDEWRPENEKAVIAKKRQMVMGATVNKRDYLKDETA
                     ASRFPTITLVAPIDIARVNELLGWEWDNVKGCAVRADEDQLIQFWLEVRHLYRSGVGFN
                     LDDETFAAAKAETAQFVDRGSYYEPVRDILLLPGNASTGQYPWDKPGYDGRFTATDVTA
                     ALRAPSSHSGRVGRALKRLADEGIVDRRNHRGTFVYWKLPDEPTGELPL"
                     /name="VapE domain-containing protein"
     gene            complement(2991..3458)
                     /locus_tag="HV074_RS26755"
                     /old_locus_tag="HV074_26780"
     CDS             complement(2991..3458)
                     /locus_tag="HV074_RS26755"
                     /old_locus_tag="HV074_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_061548400.1"
                     /translation="MKKYTPEQKAQALKLLEQDGATAATVARTMGIPPRTVRDWAKARA
                     DAPSNVLSIEEMRERAQRAVEATPQAAVRRLKNHFVQRQYELLQRHASDLQALRSASLQ
                     AMLEKDATMVKAISGLMTSLLKAQERERLIYEIKPGTEADIMRQGMNRQQS"
                     /name="transposase"
     gene            3714..3932
                     /locus_tag="HV074_RS26760"
                     /old_locus_tag="HV074_26785"
     CDS             3714..3932
                     /locus_tag="HV074_RS26760"
                     /old_locus_tag="HV074_26785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015766.1"
                     /translation="MKEFHVFKDRILAAAKESSPFWVTFEYPEDCFGYDERFVFESVSA
                     LKSMRILGLEIHNAADRRFSARFLPVE"
                     /name="hypothetical protein"
     gene            complement(4218..4805)
                     /locus_tag="HV074_RS26765"
                     /old_locus_tag="HV074_26790"
     CDS             complement(4218..4805)
                     /locus_tag="HV074_RS26765"
                     /old_locus_tag="HV074_26790"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_061301009.1"
                     /translation="MSKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELEKRKITLVSIREGVDLS
                     SAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            5066..5725
                     /locus_tag="HV074_RS26770"
                     /old_locus_tag="HV074_26795"
     CDS             5066..5725
                     /locus_tag="HV074_RS26770"
                     /old_locus_tag="HV074_26795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548402.1"
                     /translation="MTEIKKESADGAWLRSTLAELAGDAFPVYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            5908..6297
                     /locus_tag="HV074_RS26775"
                     /old_locus_tag="HV074_26800"
     CDS             5908..6297
                     /locus_tag="HV074_RS26775"
                     /old_locus_tag="HV074_26800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015768.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            6290..6652
                     /locus_tag="HV074_RS26780"
                     /old_locus_tag="HV074_26805"
     CDS             6290..6652
                     /locus_tag="HV074_RS26780"
                     /old_locus_tag="HV074_26805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061301020.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPFLHHHPGDRPAT
                     EKRAIARFKDSLAAGGQTLAFVRSDASLYALGLRFRRERTRELVASDPDVAAAGEALEQ
                     RANGVNLSYTFGGGDE"
                     /name="hypothetical protein"
     gene            6645..6830
                     /locus_tag="HV074_RS26785"
                     /old_locus_tag="HV074_26810"
     CDS             6645..6830
                     /locus_tag="HV074_RS26785"
                     /old_locus_tag="HV074_26810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151322523.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRRELPRSTGR"
                     /name="hypothetical protein"
     gene            complement(6850..7491)
                     /locus_tag="HV074_RS26790"
                     /old_locus_tag="HV074_26815"
     CDS             complement(6850..7491)
                     /locus_tag="HV074_RS26790"
                     /old_locus_tag="HV074_26815"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_061548403.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQC
                     LIDQCMRYDLDGNPTVAVTPEEAEQAKTRLTEIREFRRKARQEKDKKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(7488..8777)
                     /locus_tag="HV074_RS26795"
                     /old_locus_tag="HV074_26820"
     CDS             complement(7488..8777)
                     /locus_tag="HV074_RS26795"
                     /old_locus_tag="HV074_26820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181213195.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTSSVCADCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLALSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            9133..9342
                     /locus_tag="HV074_RS26800"
                     /old_locus_tag="HV074_26825"
     CDS             9133..9342
                     /locus_tag="HV074_RS26800"
                     /old_locus_tag="HV074_26825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
     gene            9329..10501
                     /locus_tag="HV074_RS26805"
                     /old_locus_tag="HV074_26830"
     CDS             9329..10501
                     /locus_tag="HV074_RS26805"
                     /old_locus_tag="HV074_26830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548407.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFDPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGSQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVEVMLVPDAGEAYRLAHKAAYGDDREPVPTI
                     PGASVVSFDGLNRFEPRDNLDWFDVLVEEGEDRARELLRHAVRHARLERG"
                     /name="hypothetical protein"
     gene            10732..11178
                     /locus_tag="HV074_RS26810"
                     /old_locus_tag="HV074_26835"
     CDS             10732..11178
                     /locus_tag="HV074_RS26810"
                     /old_locus_tag="HV074_26835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548408.1"
                     /translation="MKSKTELRAAATRRALEVIASEMGGWSLDGFAHGSLPNFSPLPSQ
                     QTQEGSVVLARSPFECSWAGTAVFTAQASRFVNADPASAHYEWACGVEREAVATALMVE
                     AFNVTGCVEYAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAA"
                     /name="hypothetical protein"
     gene            complement(11546..12037)
                     /locus_tag="HV074_RS26815"
                     /old_locus_tag="HV074_26840"
     CDS             complement(11546..12037)
                     /locus_tag="HV074_RS26815"
                     /old_locus_tag="HV074_26840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548409.1"
                     /translation="MAIGDDAIRDAFYIFTQQAAEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDLPTSPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAARRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCRAIQRG
                     "
                     /name="hypothetical protein"
ORIGIN
        1 caaactgtcg tgcatttttc ccgcctccgg ccagcgccct gacctctgac cgcagcgcct
       61 cgatggcagt caaaagtttg gtgtccatcc cttcaggctt cgtgccgagt ccggcgacga
      121 gcgcctctat gacggcatct gtgcgggtta ctttggtttt ctccgctcgc tgctcgatgc
      181 gctcgatcag gctgacgggc agtcgaacgg tcagggtttt cgttttctct cgcatgggct
      241 agcatctccg tgtcgtgcta tttgtcgtgc tatttgtcgt gctgtttgtc ttgcggagtg
      301 taatgcgttt tgtcgtgcgc gtcgaatttg acgtagtgca attgtcgtgc gctgaggggc
      361 gacggcgttg cgtcgccggg gtgcctgtgg acagtttctg tggacaagta ttttgcggcg
      421 gagctggccc gtggataagt ggtagaggct acgcggctgt aaggctggag ctggatcgcg
      481 tttcgcgtgg ttggatcgtt gtgtgctggt acagggggga atgtaacggt atgatgacgc
      541 ggctgaaaaa gaaaacccgt cctggcagac gggtcatttt ctttaaagct gttgttgtta
      601 aaactttaag gagctttcgc ggcgggaacc gtggaagaca ccgattctaa caacaggttg
      661 ggtggcaatc cagcttgaat tgtacgcgaa ctttccagcg cgtgcaaagg gtgttttccc
      721 cgtaaagtcc cgccgttgta gggctcctca agattcgatg tcaattttga ggatgtaaaa
      781 ttgcctaatc atgatgataa ttctggcgct gctgtagcca ctgtaacgca gatcgccgcc
      841 gccaaggcca aagctgccgc caagaccgag cgccctgcgt tcatgggtgg ttctctgcct
      901 cctgtcccgt tcaatgagtg ggatgggctc accggtagcc cgacccgcga cggtttccgc
      961 attcaggcgg ctgaaaagcg atcggtaaat ttcggcaatg agtatccact gcggtatgtc
     1021 ccgccgctga tcactgatgc tggctctggg cgctgggacg tcctcaatca cgccgacaat
     1081 ctccgctggt tgctctaccg caatggtgcc cggatggagc ttaacctcat gaccttcaag
     1141 aatgagctct catgggctga tagcggctct ctggtcgaac cggacgagca tggtgcccgc
     1201 gcctggatac tgaacaaaag cgccgaggtt ggcctgaaac gtcaggctgt tgacgatttc
     1261 atgttctacg ttgcccgttc cgctccctac caccctgttg cccgcctcct cggttcccgc
     1321 gagtgggacg gttctcctcg cgtcgatgcc gtgctggact gcgtacctgt cgcacctggt
     1381 aaggaggcgc tgcgctcggc tctgctgcgt ggactgctga ttgcggcgat caccgctgtt
     1441 gaggaagggg ctgtgtcgaa caagtacgtt ccttgcctct actccgaggc taacaactgg
     1501 tttaagacgg cgttctttcg cgctctgttc gacctgctgc ctggtgcgtt cgggcagggt
     1561 gtgtctatca acccttcgag taaggacagc gtgcgccctg cggtcatgcg ctggtttaac
     1621 gagctgggtg aagtgtcggg catgatgaag aaaaacgacc ccgatgaaat caaggcgttc
     1681 gtggagcgca acgtggatga gtggcgtcct gaaaacgaga aggcggtgat cgccaaaaag
     1741 cgccagatgg tgatgggtgc cacggtgaac aagcgcgact acctgaagga cgagacggcg
     1801 gcgtcccgct tcccgaccat taccctcgtc gcccctatcg acattgctcg cgttaacgag
     1861 ctgctcgggt gggagtggga taacgtcaaa ggctgcgctg ttcgggcgga tgaggatcag
     1921 ctgatccagt tctggctcga ggttcgtcac ctgtaccgct ctggcgtcgg tttcaacctg
     1981 gacgacgaga cctttgctgc ggcgaaagct gagacggctc aattcgtcga tcgcggctcg
     2041 tactatgaac cggtgcgcga catcctcctg ttgcctggca atgcgtccac tggccagtac
     2101 ccgtgggata agcctggcta tgacggcagg tttaccgcga cggacgtgac ggcggcgctg
     2161 cgtgccccat caagccattc tggtcgcgtc ggtagggcgt tgaagcgcct agctgatgag
     2221 gggatcgttg atcgcaggaa ccaccggggt acttttgttt actggaaatt gccagacgaa
     2281 ccaaccgggg aactcccgct ctgaaaatga cccgcttcgg cgggtttttt tatgcctctg
     2341 ccaatgttgg ggtacccgtg tgggggttcc gttttgtgtg ggggtatggg gtgatcatgt
     2401 gggggttcgg tgtgggggta acgtgtgggg gtaactggtg atgttggggt aagatgtggg
     2461 ggtaaactcg caaagaaaat ctacctccac atactcaaaa tccctttcat ggcgctgcat
     2521 gtggctttcg tgttggggta tgtgggggta cattctttta tttatctact atgtaaaaag
     2581 aggggtaata agtagccccc acaccccctg tacgcccata tgttacccct acaccctcat
     2641 acccccacac gctcaaaaat tgcgcagttg gcgtgccgca gctgcacccc tgaaggggat
     2701 gcactttgct tgccttgtga ggtggggtga tcgggggcgc agaggacgaa gctgtcgctt
     2761 cgctgggtgg tcgtctggtc gttctgcgtg agggcttgcc ctcgcgccct ctgcggcttg
     2821 tgtggaatgc gtagctgcag ctgcgcgttt ggttcgtccg ggcatcaaaa cgggacttct
     2881 cgcgcacgcg agcggtaaca attgcgtaac gtgcggatcc gcgtattccg ggggctcccg
     2941 ggttttctcc ggcggtaaaa cggcggtaaa aggcggcttt cccgccgttt tcagctctgc
     3001 tggcggttca tgccctgccg catgatgtcg gcctcggtgc ccggcttgat ctcgtagatg
     3061 aggcgctcgc gttcctgcgc cttaagcagg gaggtcatga ggccgctgat cgctttgacc
     3121 atggtggcgt ccttctccag catggcctgt agcgatgcgc tgcgtagggc ttgcaggtcg
     3181 ctcgcgtggc gctggagtag ctcgtactgg cgctgcacaa agtggttttt gaggcgtcgg
     3241 actgcggcct gtggggtggc ctcgacggct cgctgcgcgc gttcgcgcat ctcctcgatg
     3301 ctgaggacgt tgcttggtgc gtcggcgcgg gctttcgccc aatctctgac ggtgcggggt
     3361 gggatcccca tggtgcgtgc gacggtggcg gcggttgcgc cgtcctgctc cagtagcttg
     3421 agcgcctggg ctttctgttc gggggtgtat ttcttcatgg ttcctgctcc ggttggtttc
     3481 ctccggctat ttgagcgtca cgcgctgaga atggcggcaa cttttggggg gtgtacggcg
     3541 ctgtctcgtg ctgggtggta tgtcggtgcg gttttggggc tggggcgctt aggattgctc
     3601 tggtgcgttc tggtgtgtgg tgccttatgt acgtcccggg ccgactccat tatgagattt
     3661 cgttttcctg taaaatgctc gtgagcacct tcgctcttaa tgagggtatg gcaatgaaag
     3721 agttccacgt cttcaaagat cgcattcttg cggcagcaaa agagtcctcc ccgttttggg
     3781 tgacatttga gtacccggaa gactgttttg ggtatgacga gcgttttgtc tttgaatccg
     3841 tatctgcttt gaagagtatg cggatactag gactcgagat acacaacgcg gctgatcgcc
     3901 gttttagtgc ccgctttctg cctgtagagt gagtgtgctt acggtgtaag tggttcgtta
     3961 tggggtaacg cttacaccgt aaggagttcg ttatgccgcc atctcacggc gtagacgctt
     4021 catggctctg cgccaggtct gcgggttgcg gttggtgact gcgcggcgtg ccagctggcg
     4081 tgcgatgctc tgtggtgata cgatcatggt gttacctcat ttgggtgggt taacatgccc
     4141 gggttggctc tcacacttcc ccgggcttgg gttgcggcgg tggctttagc gagtaccacc
     4201 gccgcgctct ctctctctta ctccggcgcg accgcgtcga ctttctcaag atggcggtaa
     4261 atcgcgctcc ggcttaggtt tagctgtttg gctatggtgg cgatcggcac tcccatggcg
     4321 cggcgcatga caatctcccc ggcttccttc gctccgatct tggtcgggcg tccgaacttc
     4381 tggcctcgac tctttgcggc ctctatccct gctgcctgcc gttcgaggat gagcttgcgt
     4441 tccatctgcg cgaagctggc cattacgccg agtaccgctt gtccgacggc gctgctcaag
     4501 tcgacgccct cgcggatgct gaccaaggtg atcttccgct tctccagctc ctcgaccagc
     4561 tcgagggtgt cccgggcgtt cctgccgatg cggtcgatcg cgtaaatgac cagggtgtcg
     4621 ccctcctgca gcagctggtt caggaggatg ccgagggcgg ggcgctcgcg tggtggtacc
     4681 gtcccgctga tcccgtcgtc ggcaaggatg gaggcggttg ggaaccgggc gatgatggcg
     4741 tcgcgctggc tgttgctggt ttgttcgtcg gttgatacgc gaacgtaacc gtatgttttg
     4801 ctcacttttt tctctcctat gaccgatacg tctctttcga cacaacatgc cacaactgta
     4861 tcgaaagtct atcgctaaag tgtctttttc gtcgctcctg cgggtgtcct gaaatgtagg
     4921 ggtttagcga cgggctctcg ggcggctttc cgcgctgggc gagagccctg cgccctctgc
     4981 gtgggtgtgc tcggttggtc gtgacggcat gctgtctgag cggcgtttct tgccgctttg
     5041 ctgataactt gctgagaacc taaccatgac cgaaattaag aaagagagcg ccgacggcgc
     5101 ttggctacgc tctacccttg ccgaattggc gggtgatgcg ttccctgtgt atgacctccc
     5161 gtccctgcag gtagacacga actcctccat tcagctctcg gcgctcgccg atcgtcaggc
     5221 tgcgcgtgaa atgcgacagg ccgcttccga cgtggaagcg cgacggctgg acgccgctcg
     5281 agtggtggaa gggctcaaga ccgaggccga acggctgcgg ggtttgatcg ccgacggcaa
     5341 ggctgcgctg cgtgctggcg agccggtgag ccccgacgcg ggtgttgcgt cgttcctgct
     5401 gccggacatc gaggccgagc tggttgttgc tgaggctgct gaggccgacg tggcccggga
     5461 gcgtgacacc ctgctgcagg atgccgatcg ccgtgacgcg gcggctgcgc tggcgctgtt
     5521 caactgggcg cactccgttc gcgtgcagcg tatcgagctg cttctcaagc tggcgatgga
     5581 cgaggcgacg acgctcgctg aaaccgacgg cgggcgcggc ctgtaccgta ccgtgatcgc
     5641 gcctgaccgc cgtcttaacc aaattttcgc cacgcagggc gctattgaaa tcctgcgccg
     5701 taaccgtggc atggagggtg tgtaatgtct gctttcaaac tcctgtgtga ccggcttcgc
     5761 aatgaggcga cgctggcaaa atctgacggg gccggcgctt tcaaaaagca atctctggat
     5821 cccgacctga agggcggcaa gaagggcaaa aagcccgctg gggagctgga ggaggacgag
     5881 gcgatcgcgt ctctccaccg taagcgcatg aacgacgtta aaggttgcaa gacgctgacc
     5941 aaatccaccg gcgctgatga ctttggcctc cctccgctgt cgtcggtggt cgagacagtg
     6001 gtggagacag gcggcgcagc gcagacgttc gcgccgatcg gggctccggt ggcgtctccg
     6061 accgaagcgg ttcaccgtgc gcgtgcgcag tccatccagg acgcggcgtt cctgaacgag
     6121 atgaaagcgg cgggtgtggc accggctgac ctggtgttac ttgacggcgt gttgacgtcc
     6181 tctcgtggga tgccagacgc ggcgcgtgct ccggcgtttg ccgaggtgcg ccgtcaggtt
     6241 caggcgggcg gcatgacgct gtcaccggct gcgcgtggta ttgactggaa tgcgtaagct
     6301 gccgcgtcac ctgcagggcg aggtgttgat gccgctgtcc agcaggatca cgcgctcgca
     6361 acggctggag ctgatctggc tgttgccgtt cctgcatcac catccgggcg atcgtcctgc
     6421 caccgagaag cgggctattg cccggtttaa agactcgctc gctgcgggtg gccagacgct
     6481 ggctttcgtc cgttcggatg cgtcgctgta tgcgctgggg ctgcggttcc ggcgtgagcg
     6541 cacccgggag ctggtcgctt ctgatcctga tgttgctgcg gctggcgagg cgctggagca
     6601 gcgtgcaaac ggtgtgaacc tgtcctatac gttcgggggt ggcgatgagt gaatttgctg
     6661 tctcgcgcct ggtcattcct gaggtggtga tccccgatgg ggtcattgct gcccgacggg
     6721 caaggggaca ggctcaacgt gtcgcagctg ctcgccgtgc tgcagagcgt cgtgctgcgc
     6781 ctacgtggcg ctgggtgaga cgggaactgc cccggtcgac agggcggtga aacggtgaga
     6841 cggggcgcgt tatgcgccct tttttttgtc cttctcctgg cgtgccttgc gtcggaattc
     6901 ccggatctct gtcaggcggg ttttggcctg ttcggcctcc tctggcgtga cggcgacggt
     6961 ggggttgccg tcgaggtcgt agcgcatgca ctggtctatg aggcactgct ggtacgagct
     7021 ggtacccgtg acaatgccca ggatgcgacg gacggctttg cggtctgcct ctggcggcat
     7081 gtccagctct gcgcctcggg tcaggatctg ctcgatgatg ccctttgcca tggggcgacg
     7141 gtcaactccc cgggtcagca agtcaggcca gtgctctttc aggaatgctt tggtcacccg
     7201 gttggccagc gcctttttgg acttgttggc gatcgcgatg tcctgctggc ggcggtcgtt
     7261 ctcctcctgt ctgcggatcc cctcggcgac ctgctcgggc gtctttttcg gctttgccgc
     7321 tgtgggcttc tgtgcgggct tctgcggggc tttcgctttt ggctgtacct tcgccttgct
     7381 ggtagtccgt tcggctggct gcggctcaga tttcgcggcc ttttccggcg ggacgtagac
     7441 cgtgcggttg cgtcgcttga tggtcaggat cggacgggtg gtttcggtca taaatctctc
     7501 tctggtcggt tggtccggat gtggtcggca agggcgatca gggtggcggc gtcgccgcgg
     7561 tcccgatcga tgcgccggat gatgtcgttg atgtagtccc agcgcccgac ggcggcaagg
     7621 acggaaaggg cgaggcggtt gaaccggctc tctggtaccc ggtcgagggt cagggggatg
     7681 cgcttgcggg ggcgtcctgg accgcgccct gtccctggtc ttttgagcag ctgcggctgc
     7741 tccctctgcg gcaaaataac ggggatcttc gccaggctgc gcgtctcgtt ctgcgcgaac
     7801 tcgggcaccg cgatgtgctc gtctgtccat gtcctgcctc cgggcctggc tgatgcgctg
     7861 gagtcgcagg acgggcagga gacgacgatc tcccgtcgcg gcttgccctt gaccggtgcc
     7921 ttgctgatca ccaggggcgc ggctttgcaa tcagcgcaga ccgacgatgt ccagaccgcg
     7981 ttgtctggca gggtggcgtt ccggtactgg tgcgcggcgt catgcggtgt ctggaagctg
     8041 accgtctgaa acgtgggggc gacgctgagg ggtgccgccc atggctggca cctgggacaa
     8101 gcaagctcca cctctccggc gtgcccgtcg ccaccgttcc agcgcagggg ctcgcggtgt
     8161 tcggggcagt agtacacgaa accgtcgcgg cgtgtttgcc cgaccgggcc tggtcggcga
     8221 tcgtggaacc gtgcgcccgg gttggctcgc atggcgcgga cgagcgcctc gccctggtgg
     8281 atgccgtacc agacctgcca gccatcgccg atgccgatca ggaacggcga gacgaacggt
     8341 ggggtgcggt tgactctccc gcgtgcgttg gctccgatgg tggcttcgcg ttcgcggttc
     8401 cagcaggaga cgcacaggct cgaaccgggc ttattggggg cgatcaggcg gttgtctttc
     8461 cggctgcaac gggggcagag cttacgggtg tctgccgggg tggtcagtgg tgcgtcaggg
     8521 ggctgttcgg taccgaccca aagcgcgagg ccagtggggc accggtggca ctcgaggtgg
     8581 gggttttcaa tcgtgcgatc gaggtggctc tgctcataaa tctgtcggca tgcctcgacc
     8641 cggagggagc acccgccggg gcaggtgacg aacaaaacgc cggggagctg ctcggggaat
     8701 ttttcagtcg cgcttacgcg ttcgagtgct gccgggttcg gctgcgtcga ttcgttcgtg
     8761 tcgttatcgt cgttcactgc tccccctgcg tcgaaatggt cgccagaccg cgccagcact
     8821 ggctatgagt cttttttagt atctcacaaa aaacgccact ttggcgttaa ttgtgttacg
     8881 cttgtcttac tgattcctga cgagcagttg ggaaacatga aaccggatcg cccggtgccg
     8941 ctttccctcc tccccaacga ggcggggttg taaacgagga tgcgcgcttc gtccttggca
     9001 ggtgcgagta agcgagaagg gctgctgtcc tgccgctatg cggtaagtcc tcaagggcgg
     9061 ctttatcgat aatcgtcact cccgcccccc gatgcccgtt aagctgaggg gggcacctat
     9121 ctggtactcc caatgaaaaa acgaatctat accgctgacc tcgctgcgct gctgaacgtg
     9181 tcgctgcgca cgctctcgcg gctaagttcg cgggggatcg tgccgactcc ggcagggcgc
     9241 tccctccgtg gcgggcatta ctggctggct ggcaatcccg agatcaaacg cttcgttgcc
     9301 gaccagcgtc gtccggggag ggctgctgat ggcgttcgtt gatttcgaca ctacccatct
     9361 gaaagaggat ccgggcctgt tctggcgtgc cgtatcggct gcgggtgctc cggctgatcg
     9421 agacgaatgg gtgcgtctct gcctggctct tggttgctgg gcgtctctct gcggcgagga
     9481 cgggctggca gctatcgagg cctggtccac tcgacatggg cgaggcgaga atcgccatcg
     9541 ccagcagtac aggtcgtttt taaaaggcga cctggataca ttcgccgctc aggctttcct
     9601 caatttctcc ggcgtcgaat ggcgcaacgg tcgcgcttct gtccagtccg acgtcgatcg
     9661 cgctgaacgg ctgcggttgt cccgtgaggc cacagcgaag gcaaaggctg aacgcgacaa
     9721 gaagggtgat gctgctgcgc gtgctgcggt caagctgctg gcgaagggag gggatcctgc
     9781 gtctacccca tactggcgca aaaagaccgc tctggcggct gcgggtggcg tccagctgcc
     9841 cgccctcagc acagatcttg gtgttggcgg gatgtttcga tcgttcgatc cgcgtgctta
     9901 ctggtctgat tgcctcctga ttccggttta caagttcgac gccgcccgtc agctgtatgt
     9961 tgacggcctg gaggccatct gcgggcgctg ctcggctgag ggttcccagc tcactggctc
    10021 gaagttctct ccggcgggta cccgcaagca gggcggcttc tacccgatgc cggggttcat
    10081 ggacgtgctc gacggctccc cgcacgcgcc ctactcgcct ctgttcgtct gtgaggggtt
    10141 cgctacggcg ttcgccctgc accacctgag ccgtggcaag gtggcggtct gcgctgcgtt
    10201 ctcgatcgcc gggttcggca gctgcgtggc gtcgctgcgt cggttctggc ctgatgttga
    10261 ggtgatgctt gtaccggacg cgggagaggc gtaccggctg gcgcataaag cggcctatgg
    10321 cgacgatcgc gagccggtcc cgacaattcc aggggcgtcg gtggtgtcgt tcgacggcct
    10381 gaaccgcttc gagccccgcg ataacctcga ctggttcgac gtgctggttg aggagggcga
    10441 ggatcgggcg cgtgagctgc tccggcacgc ggtgcgtcat gcccggcttg agcggggctg
    10501 agtggcgtgt ccgaatggtg cgcctttcgg gacacccttc ctgctaccgc cgctccggct
    10561 tttgcgaccg actttcgaga cactatcgcc gcttttttgc agagacggat taaagcgacg
    10621 caccgccgct gcggtcgtga cgctatcctg cagctggttt ggtttccagc agcacactca
    10681 agcccggcac cgttttggtg tcgggctttt tttcgcccgt aaggagggca catgaaatca
    10741 aagactgaat tacgcgctgc cgcgacccgt cgcgcccttg aggtgatcgc ttctgagatg
    10801 gggggctggt cgctcgacgg gtttgctcat ggatcgctgc cgaacttctc cccgctgcct
    10861 agccagcaga cgcaggaggg ctcggtggtg ctcgctcgat cgcccttcga gtgttcgtgg
    10921 gctggcactg ctgtgttcac cgctcaggcg agccggttcg tgaatgccga tccggcgtct
    10981 gctcactacg agtgggcgtg cggcgtcgaa agggaggctg tggccaccgc gctgatggtt
    11041 gaagcgttca acgtgaccgg ctgcgtggag tacgccgggt tgcctcctgt ggacggcatg
    11101 gtgctgctga ccatggacga ggccgacgtg gagctgatca gggcggcgat gctgccttgg
    11161 ttagatgcgg cggcgtaggc cgtcgctcct ctgcgtcaga tcaggccacg ctgacgcaga
    11221 ggctggatac gctagatggc tacggctaaa gccacaggtg acgcgggctc attgagttca
    11281 ggagagcatt atcattccac ttttggttga atcttagctg taatccgtat agaattattt
    11341 tactgcaaga gcgttctaca gatacgtaaa ttattctgta gtcagcagta aggagctaaa
    11401 actcctgacc aatagcgaat ttgatagaca gcagaaaacc gccgatgctg ctagatccga
    11461 aaggaaccaa tgaagggaaa tatttaggcg ttatggttca ttctcattaa attgaggaga
    11521 actgcttaaa tgggcgtcct tcctatcacc ctcgctgtat agctctacac ttggcctccg
    11581 ttgcatggta tgcagttacg ggggccaaat tgttttcaga gacatcaact atcgccattg
    11641 cgaaaaaacc agccccagcg acgttaatac gacgagcagc attcagcaca atagtctgct
    11701 cagaataatc cctgttaacg tagacgttac tctgcactga aaatgcagta gccaaaattt
    11761 ccgcacactc ctgccaactt aatttatcct tcgagttacg tgcagatagc acatgcctaa
    11821 acatagccca agtaattggt attgctggcg acgtaggcaa atcccagccg tttctctgac
    11881 aaacaacctt catctgattg aattgttttt tagtcatcaa ataagtgaaa caaggagtct
    11941 cccagacttc ttgagggagc cccttatcat ccatttccgc agcttgttgc gtgaagatgt
    12001 agaaagcatc ccggattgca tcatctccga tagccactga ctttccttat aaataattta
    12061 aggctgcatt ttatcatatt taattacctc acttaggact gctaactcag cacaatagac
    12121 cactctcccg cagcttatgg cggtagtccc ggacgcggct ggtgttccac tcctgccccg
    12181 tcgctgtccg gtacccggct gcgttcagcg ccgacgcgat ggcgggcagg gtggcgttct
    12241 ccccggtcat gctgacgatc agctggtcgg cctcatcgct gacggcctgg aagcgtggat
    12301 cggctccccg ggggactatc tgtcgtgcgc tttgtcgtgc ggtttgttgt g
//
