LOCUS       NZ_CP055541             6449 bp    DNA     circular CON 30-APR-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00398 plasmid pRHBSTW-00398_4,
            complete sequence.
ACCESSION   NZ_CP055541
VERSION     NZ_CP055541.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148600
            Assembly: GCF_013612875.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00398
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 102.020446088887x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013612875.1-RS_2024_04_30
            Annotation Date                   :: 04/30/2024 03:57:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,350
            CDSs (total)                      :: 5,235
            Genes (coding)                    :: 5,084
            CDSs (with protein)               :: 5,084
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 45 of 151
            Pseudo Genes (incomplete)         :: 109 of 151
            Pseudo Genes (internal stop)      :: 37 of 151
            Pseudo Genes (multiple problems)  :: 31 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055541.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00398"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00398_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6332..6449,1..296)
                     /locus_tag="HV183_RS26560"
                     /old_locus_tag="HV183_26565"
     CDS             join(6332..6449,1..296)
                     /locus_tag="HV183_RS26560"
                     /old_locus_tag="HV183_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181219994.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISNGKLSICTDENGIKKIDTSE
                     LLRVFGAFKAVHHEQSDNVTSEQHVTPNGTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPATQEPATQPVEKKSWRFWKK"
                     /name="hypothetical protein"
     gene            complement(508..1188)
                     /locus_tag="HV183_RS26565"
                     /old_locus_tag="HV183_26570"
     CDS             complement(508..1188)
                     /locus_tag="HV183_RS26565"
                     /old_locus_tag="HV183_26570"
                     /inference="COORDINATES: protein motif:HMM:NF022971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_181219995.1"
                     /translation="MKKNILLVEGSNDKAFFERVCKKHGLNYTVQVSNPVDFVPSSQGG
                     FNSKQGVINSLTTLLPLLEDEDSSINKIAIILDADITGSNNGGFSSTISQIEVKAGEFK
                     YNNHSHCNPGVELKHSDKNMHSLGVWIMPDNKSDGAIEHWIKDKIIESERKLFEHACDI
                     VSKLPVTKFSDSSIAKAELATWLSWQNQPGRTVAYTLKDGNELIDINDIKYKNLIDWLK
                     AFFN"
                     /name="DUF3226 domain-containing protein"
     gene            complement(1185..2354)
                     /locus_tag="HV183_RS26570"
                     /old_locus_tag="HV183_26575"
     CDS             complement(1185..2354)
                     /locus_tag="HV183_RS26570"
                     /old_locus_tag="HV183_26575"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_181219996.1"
                     /translation="MMLKSVYISNFKGLKEISIPSVGNLNLIVGKNNSGKSSLLEALML
                     YASGGSESVLHELSVSHDEVSRERFRPTEYQQTDSESQSFITNEMLPYEHLFYGRTFDQ
                     REPIHIGESESERSTKSLVISFMKDRKRNDKSNDEAIKLLKQLNIFKDDIVMPDIRSQN
                     VLEVKKGKSKHFIPLDEAVFRTSRLVDSDDRSKINFRYVPTKITSMDDLGILWDQITLT
                     QYEKITIEALQSIEPNISDIRFILKDGSRRQRTAIVKLSGSDDVFPILSMGDGIGRILQ
                     LILNMYAARDGIYLIDEFDNGLHYSIQKSLWKMIFNLSKVLNVQVFATTHSWDCVQSFS
                     EVSIENKDLNGFLLKLGKSKLSTNKGNSIVTIFNEDKLYTITQEMVEVR"
                     /name="ATP-binding protein"
     gene            complement(2429..3010)
                     /locus_tag="HV183_RS26575"
                     /old_locus_tag="HV183_26580"
     CDS             complement(2429..3010)
                     /locus_tag="HV183_RS26575"
                     /old_locus_tag="HV183_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="WP_181219997.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQTESAQLSRQTAQLAQ
                     VIRELDSASGNMTETVRKSVSTALNQVKQELKQAGVEQQKPAVNELNQVVKAANESVSA
                     MRREMSRYTWKSAIYIVLTILAVMGGCLWGMYYFLDSGYARIAEMQKMEAVWQKKAPLA
                     DISNCNGKPCVKVDTTQAYGDKKDTYYLFK"
                     /name="tRNA modification GTPase"
     gene            complement(3023..4132)
                     /gene="mobA"
                     /locus_tag="HV183_RS26580"
                     /old_locus_tag="HV183_26585"
     CDS             complement(3023..4132)
                     /gene="mobA"
                     /locus_tag="HV183_RS26580"
                     /old_locus_tag="HV183_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_181219998.1"
                     /translation="MASYHLSVKTGGKGNASPHADYISRDGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEHLVAQRERWAELQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQSILARREAERQAQQSRDERDCVIDVTTSLREAIRERDDASVKPAQQPEPEVA
                     AGITFDFEKEPDKLNALVSDAMKDVQEEIDLQSLVNDAMAEFQGIHQEMERQRERERLA
                     GKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            4297..4764
                     /gene="traD"
                     /locus_tag="HV183_RS26585"
                     /old_locus_tag="HV183_26590"
     CDS             4297..4764
                     /gene="traD"
                     /locus_tag="HV183_RS26585"
                     /old_locus_tag="HV183_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_181219991.1"
                     /translation="MAKSEKQEQWAVDRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSHTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTA"
                     /name="traD"
     gene            5368..6324
                     /locus_tag="HV183_RS26590"
                     /old_locus_tag="HV183_26595"
     CDS             5368..6324
                     /locus_tag="HV183_RS26590"
                     /old_locus_tag="HV183_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_181219993.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPMIENPTIAE
                     RTVTFTYQQYAQMMGLTHDNAYQRLKSSTRELMTRTVEIIYPTGAISERIFQWVNYAEF
                     NRETQSLRLVFSEYILPYLFQLKRFIKYNLEHVKAFENKYSMRVYEWLLKELTQRKTHK
                     ANIEISIDEFKFMMMLENHKSYELYKEFNRKILSPINKDINTYSNMTLSIEKYGRPAQT
                     LIFQVELDKQIDLVTELAKDISDNKIPVATTPETHLHNGLRKTLNDALTAKIQLTSFEA
                     KFLSDMQSKYDVNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     Typing          6149..6324
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.915"
                     /coverage="100.0"
                     /evalue="2.05e-72"
     Typing          complement(3027..4132)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.16"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agatagacac atcagagctt ttgcgagttt tcggtgcatt caaagctgtt caccatgagc
       61 agagtgacaa tgtaacatct gaacagcatg taacacctaa tggcacaggt gaaaccagta
      121 aaacaaagca actagaacat gaaattgaac acctgagaca actcgttaca gctcaacagt
      181 cgcacataga cagcctgaaa caggcgatgc tgcttatcga atccaaactg ccagcaacac
      241 aggaacctgc cacgcagccg gtggaaaaaa aatcatggcg attctggaag aaatagcggt
      301 ttcagccagt aaacaggttg aaaccaatcc cgcgatcctg ataacaaacc ggcaacataa
      361 aagcagcccg ttttcggggt acaaaacccg cgcttttgga cgttccggcg ggttttgaag
      421 gtgagtggta tggcggtggt gcgcgcaagg gatgttatgt tcaataatga ggcacctaga
      481 atattaagat atacatacga cctataatta attaaaaaaa gctttcaacc aatcgataag
      541 gtttttatat ttaatatcat taatatcgat taattcatta ccatctttca atgtataagc
      601 aacagttcgc cccggttgat tctgccatga aagccatgta gctaattcag cctttgcaat
      661 tgagctatca ctgaacttag tgactggtaa tttactcaca atatcacaag cgtgttcaaa
      721 aagcttacgt tctgattcta ttattttatc ttttatccaa tgttcaattg caccatctga
      781 tttattatca ggcataatcc aaacacctag agaatgcata tttttatcac tatgtttcaa
      841 ctcaacccca ggattacagt gggagtgatt attatattta aattctcccg cctttacttc
      901 tatttgactt atcgtgctag aaaacccacc attattactc cctgtaatgt cagcgtctaa
      961 aataatagct attttattaa tggatgaatc ttcatcctct aataacggca atagcgttgt
     1021 taaactatta ataacacctt gttttgaatt aaacccaccc tgactagaag gaacaaaatc
     1081 aacaggattg ctaacttgca ctgtatagtt caacccatgt tttttgcaaa ccctctcaaa
     1141 gaaagcttta tcattactac cctcaacaag taatatgttt tttttcatct tacttcaacc
     1201 atctcttggg ttatagtgta tagcttatct tcattaaaga tggtaactat agagttcccc
     1261 ttatttgtag ataacttact cttacctaac ttaagtaaaa aaccatttaa atctttattt
     1321 tctattgata cctcagaaaa actttgaaca caatcccagc tatgcgtagt tgcaaaaacc
     1381 tgaacattta aaaccttaga tagattaaaa atcatcttcc acaatgattt ctggatactg
     1441 tagtgcaatc cattatcaaa ctcgtcaatc aaataaatac catccctcgc ggcatacata
     1501 tttaatatta attgcaaaat cctaccaatg ccatcaccca tacttaaaat tggaaaaaca
     1561 tcatcacttc cagataactt aacaattgca gttctttgcc ttctactacc atctttcaat
     1621 ataaatctta tgtctgatat gtttggctca attgactgca atgcttctat tgttattttt
     1681 tcatattgag ttaatgttat ttgatcccat aaaatcccca aatcatccat tgatgtgatc
     1741 ttggttggga catatctaaa atttatttta gatctatcat cagagtcaac aagcctacta
     1801 gttctaaaga ccgcttcatc aaggggtatg aagtgcttag attttccctt tttaacctca
     1861 agaacattct gagatcttat gtcaggcatg acaatatcat ccttgaatat attcaattgc
     1921 tttagcaact taatagcttc atcgttagac ttatcatttc tttttctatc tttcatgaaa
     1981 gatataacta acgatttagt tgatctctca ctctcactct cacctatatg tataggttct
     2041 cgttgatcaa aagttctacc ataaaataaa tgctcgtatg gcaacatctc atttgtaata
     2101 aatgactggg attcactatc tgtttgttgg tactccgtgg gtctaaaacg ttctcttgat
     2161 acttcatcat gagatacaga tagttcatgt aataccgact cactacctcc tgacgcatac
     2221 aacattaatg cttctagaag agagcttttc cctgagttat ttttccctac aataagattc
     2281 aaattaccaa cgcttgggat acttatttct ttcagtccct taaagttaga aatgtaaacg
     2341 gattttagca tcattttacc tattataaag cttattaaat ttaattaagg caaccatata
     2401 aaatcaccta tatataaatc tgtatctact acttaaaaag atagtatgta tcttttttat
     2461 cgccatatgc ttgcgttgtg tctactttaa cacaaggttt accattacaa tttgaaatgt
     2521 cagcgagcgg ggcttttttc tgccaaacag cttccatctt ttgcatttca gcaatccgtg
     2581 cgtagccgct gtctagaaag taatacatcc cccacagaca tcctcccatg accgccagta
     2641 tcgtcagaac gatataaatc gctgacttcc acgtatagcg cgacatttcg cgtcgcatgg
     2701 cggacacaga ttcattagcc gctttaacta cctgattcag ttcattcaca gcaggtttct
     2761 gctgctccac ccctgcttgc tttaactctt gcttaacctg gttcagggca gtactgacag
     2821 attttctgac agtttcggtc atattaccgg aagcactgtc cagctcacga ataacctgag
     2881 ccagttgtgc tgtctggcgg cttaactgcg cagattcagt ctgaaactgt tctgtcagag
     2941 aagtaacacg cttttccagc gcctgatgat gcccgataag ggcggcaatt tgcttaattt
     3001 cgtcgctcat acaaaatcct gtctatcgtg agaatgacca gcctttatcc ggcttctgtc
     3061 gtatctgttc ggcgagtcgc tgtcgttctt tctcctgctg acgctgtttt cccgccagac
     3121 gttcgcgctc tctctgcctt tccatctcct gatgtatccc ctggaactcc gccatcgcat
     3181 cgttaacaag ggactgaaga tcgatttctt cctgtacgtc cttcatggca tcactgacca
     3241 gtgcgttcag cttgtcaggc tctttttcaa aatcgaaggt gatgccggca gcgacttccg
     3301 gctcaggttg ctgcgccggc ttaacggacg catcatcccg ttcacggatg gcttcccgca
     3361 gcgaggtggt aacatcaatg acgcagtccc gttcatcacg ggactgctgc gcctgtcttt
     3421 ccgcttccct gcgcgcaaga atggactgta actgttcagt atcgaaccgc tgaacctgac
     3481 ccgcgcccag atgccgctca ggctcacggt caatgccctg cgccttcagg gaacgggaat
     3541 caacccggtc agcgtgctga taccgttcaa ggtgcttatt ctggagttca gcccagcgtt
     3601 cccgctgggc aacaaggtgt tctttgcgtt cggtcggtgt ttccccgaaa cgtgcctttt
     3661 ttgcgccacc gcgctccggc tctttggtat tagcccgttt aaaatactgc tcagggtcac
     3721 ggtgaatacc gtcattaatg cgttcagaga acatgatatg ggcgtggggc tgctcgccac
     3781 cggctatcgc tgctttcgga ttatggatag cgaactgata ggcatggcgg tcgccaattt
     3841 cctgttggac aaaatcgcgg acaagctcaa gacgttgttc gggttttaac tcacgcggca
     3901 gggcaatctc gatttcacgg taggtacagc cgttggcacg ttcagacgtg tcagcggctt
     3961 tccagaactc ggacggttta tgcgctgccc acgcaggcat atttccggac tccttatgct
     4021 caaggtcgga gtctttttca cgggcatact ttccatcacg cgaaatgtaa tcggcatggg
     4081 gagaggcatt gcctttcccg ccggttttta cgcttaggtg ataggatgcc atcgtgtttt
     4141 atcccgctga aggcgcgcac cgtttctgaa cgaagtgaaa aaacgtctaa gtgcgccctg
     4201 ataataaaaa agttatcagg gattgtagtt gaatttgacc tccgctgcca tcactgagca
     4261 taattatccc gttaaaattc agagggtaaa acagtcatgg ctaaaagcga aaaacaggaa
     4321 caatgggcag tagacagggt gcagtacatt cgcggattaa agtcaccgaa tgagcaacag
     4381 aaactcatgc tgatactgac ggataaagcg gataaaacag ctcaggatat caaaacgctg
     4441 tccctgctga tgaaggctga acaggcagca gaaaaagcgc aggaagccag agcaaaagtc
     4501 atgaacctga tacaggcaga aaaacgtgca gaagccagag ctgcccgtaa agcccgtgac
     4561 catgccctgt accagtcagc cggactgctt atcctggcgg gtctggttga cagccatacg
     4621 ggtaagcctg ttgatgatac cgctgccttg ctgggtgcat tagccagtct gaatgacctg
     4681 tcgagggata atccgaagtg gtcagactgg aaaatcagag gtcaggaact gctgaacagc
     4741 aagaagtcag acagcactgc atagttgacc tgccataaaa cgccctgaga atccctctgc
     4801 gggcttttct tgtttcatga gtagatttcc tcgcatgact tcatgaaaag cgactgtggc
     4861 gctatttacc cccattccct gccagagctg tgagcgaagc gaactgattt ccacagagat
     4921 aacagcgatt caggagctgg atggtcggag acaaaagaaa tgttcagcga gttgcccgag
     4981 cttgcgaggg tgctatctca gtattttggg ggtttaaggt ctgttttgaa gaggagcaaa
     5041 cagcgtttgc gacatccttt tatcatatcg ttcaactgac tcaggcgtgc agttatccac
     5101 aaggctggga tctattcttt ttatcttttc tattctttct ttattctatt gtttttaatt
     5161 gattgttttt aaaggtaaaa aaatgagaaa agtgtactaa aaatcggaat tggtgtacta
     5221 aaaatcggaa tagaggacag aaaggtgtac tgaaaatcga acaatccaca actcaagccg
     5281 ggtaggtgta ctatttttca atggtacacc aatgagcatt ggtgtaccat atgctataag
     5341 tacacttaaa acccaaaaat aactatcatg tctgaattag tggtattcaa agccaatgag
     5401 ttggcgatca gtcggtatga tttaactgaa catgaaacaa agcttatttt gtgttgtgtc
     5461 gctttgctaa acccaatgat tgagaatcca actatcgcgg agcgaactgt tacatttacc
     5521 taccaacaat acgctcaaat gatgggattg acgcatgaca atgcttacca aagacttaaa
     5581 agctcgactc gtgagttaat gactagaacg gttgaaatca tctacccaac tggtgcaatt
     5641 tcagaacgta ttttccaatg ggtaaactac gcagaattta accgagagac tcaatcgctc
     5701 cgtctagttt ttagtgaata cattttgcca tatctgttcc agttaaaacg attcataaag
     5761 tacaacctag agcacgtcaa agcctttgag aacaaatact ctatgcgtgt gtatgagtgg
     5821 ttactcaaag agttaactca acgaaaaact cacaaggcga atattgagat cagtattgat
     5881 gaatttaaat tcatgatgat gttagaaaac cataagtctt atgaattata taaagaattt
     5941 aacaggaaaa tactctcacc aattaataaa gatattaata cttacagcaa catgactctt
     6001 tccattgaaa aatatggcag accagctcaa acactgatat tccaagttga attagacaaa
     6061 caaattgatc ttgtaacaga actagccaaa gacatatccg acaacaaaat accagtggca
     6121 accacaccag aaacgcatct gcataacgga ctaagaaaaa cgctaaatga tgcactcact
     6181 gcaaaaattc agctcaccag ttttgaagca aaattcttga gtgacatgca aagtaagtat
     6241 gatgtcaatg gttcgttctc atggctcacg caaaaacaac gaaccacact agagaacata
     6301 ctggctaaat acggaaggat ctgaggttct tatggctctt gtatctatca gtgaagcatc
     6361 aagactaaca aataaaagta gaacaactgt tcaccgttac atatcgaacg gaaaactgtc
     6421 catatgtaca gatgaaaacg gtataaaaa
//
