LOCUS       NZ_CP055516             1919 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00472 plasmid pRHBSTW-00472_8,
            complete sequence.
ACCESSION   NZ_CP055516
VERSION     NZ_CP055516.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148624
            Assembly: GCF_013611495.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00472
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.623145233813x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013611495.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:23:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,090
            CDSs (total)                      :: 5,963
            Genes (coding)                    :: 5,757
            CDSs (with protein)               :: 5,757
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 75 of 206
            Pseudo Genes (incomplete)         :: 133 of 206
            Pseudo Genes (internal stop)      :: 33 of 206
            Pseudo Genes (multiple problems)  :: 27 of 206
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055516.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00472"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00472_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(811..993)
                     /locus_tag="HV207_RS30160"
                     /old_locus_tag="HV207_30170"
     CDS             complement(811..993)
                     /locus_tag="HV207_RS30160"
                     /old_locus_tag="HV207_30170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            complement(1219..1455)
                     /locus_tag="HV207_RS30165"
                     /old_locus_tag="HV207_30175"
     CDS             complement(1219..1455)
                     /locus_tag="HV207_RS30165"
                     /old_locus_tag="HV207_30175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(1620..1793)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          55..568
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.517"
                     /coverage="100.0"
                     /evalue="4.73e-158"
     Typing          1760..1919
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.925"
                     /coverage="99.0"
                     /evalue="2.5300000000000007e-56"
ORIGIN
        1 ttccataggc tccgcccccc tgacaagcat cacgaaacct gacgctcaaa tcagtggtgg
       61 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc
      121 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttatct
      181 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
      241 caaacccccc gttcagtccg actgctgcgc cttacccggt aactatcatc ttgagtccaa
      301 cccgagaaag cacgacaaag cgccactggc agcagccact ggtaactggg atatacgaga
      361 gagagattca gaggtcttga agtatgggcc tgactgcggc tacactgaaa gaacagtttc
      421 ggtatctgcg gtctcgtata cccagttacc tggttaagca gttccccaac tgacttaacc
      481 ttcgatcaaa ccacctcccc aggtggtttt ttcgttttca gagcaagaga ttacgactga
      541 aacgacagga tcacaagaag atcattaact ccgagagtag taacccccac catgtttgcc
      601 tatcaacaca cttaggaaac attacttgcg tttagttata cgacttctag ctctccataa
      661 cagatcctca ttttctgctg atggatttcg acggtatcgt tcctccgctt tccgtactcc
      721 atattcagga aacaccctga cagcaaccat ttgtataaag acataacaca ccaaaagcaa
      781 aaataaaaaa ccaataaact caaacatata tcaccctctt ttcttctcat ttctcatgcg
      841 tgtagccacc attattttat agttagcact cgtagggttc tccatatatt tacgggtagc
      901 acgttcgacg gcagattcgg atgaactaaa aaaaagcata aataatttaa atgcaataac
      961 ggcgactacc actacaccaa taaatgtaag catcactctt actcctgtta tcaacattaa
     1021 acataacatc cgttggccgc accgcctcaa acgccctcag cgcgtttctg aggcctccgg
     1081 cctgaaacat acacctttct gtgcccatat cgagaaaaaa cgcccctgat gatttctgcg
     1141 tacctgcatg gctatgcacg cgggtgaatg gcagacgatt tcggagtgtc agcaccacta
     1201 aaccgccaaa aggcgcaatc agcggcgcgg cgcgcgcgga cgtgaccagg caaactcgga
     1261 ctgctgccgc tcatcctcac gtaatgcacg ctggcggtcg gcgtaacgct gcgactcaat
     1321 ctgcgcacgc gctctgttca ggacgatatg ccggttcggc tgatttagct ttttgcctga
     1381 cgctctctca gctgcggcaa tagcatcatc aagaaatttt ttctctgcag gtgtcaggtt
     1441 tgtcagtctg ttcatagcat tctcccgtca gaataactcc gcacagtctg ccagacacag
     1501 cagaaccgat gaagcgctgg cggtgctgaa agacagccag aaacggtgaa cgggagcgga
     1561 ttttaatccg gtgacgtgag acgttgggca agccgcaggc gcggcagtgg ttttagcggg
     1621 tttcggggcg cagccctgaa ccagtcacgc agcgctagcg gagtgtatac tggcttacta
     1681 tgttggcacg gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa aaaggcggca
     1741 ccggcgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct cactgactcg
     1801 ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc
     1861 ctggaggatg ccaggaagag acttaacagg gaagcgatag ggccgcggcg aagccgttt
//
