LOCUS       NZ_CP055515             2927 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00472 plasmid pRHBSTW-00472_7,
            complete sequence.
ACCESSION   NZ_CP055515
VERSION     NZ_CP055515.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148624
            Assembly: GCF_013611495.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T2-00472
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104.623145233813x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013611495.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 03:23:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,090
            CDSs (total)                      :: 5,963
            Genes (coding)                    :: 5,757
            CDSs (with protein)               :: 5,757
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 75 of 206
            Pseudo Genes (incomplete)         :: 133 of 206
            Pseudo Genes (internal stop)      :: 33 of 206
            Pseudo Genes (multiple problems)  :: 27 of 206
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055515.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00472"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00472_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(354..701)
                     /locus_tag="HV207_RS30140"
                     /old_locus_tag="HV207_30150"
     CDS             complement(354..701)
                     /locus_tag="HV207_RS30140"
                     /old_locus_tag="HV207_30150"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110958373.1"
                     /translation="MIMILWWISSFITHMMKVGNVREYGPTKSHLLLMVTTLPAGVGVF
                     IYPIVEVIWGDLSLLKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIA
                     GLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(716..1093)
                     /locus_tag="HV207_RS30145"
                     /old_locus_tag="HV207_30155"
     CDS             complement(716..1093)
                     /locus_tag="HV207_RS30145"
                     /old_locus_tag="HV207_30155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110982627.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            complement(1383..1526)
                     /locus_tag="HV207_RS30755"
     CDS             complement(1383..1526)
                     /locus_tag="HV207_RS30755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_085409091.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRNV
                     EV"
                     /name="replication initiation protein"
     gene            1942..2133
                     /locus_tag="HV207_RS30150"
                     /old_locus_tag="HV207_30160"
     CDS             1942..2133
                     /locus_tag="HV207_RS30150"
                     /old_locus_tag="HV207_30160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2133..2555
                     /locus_tag="HV207_RS30155"
                     /old_locus_tag="HV207_30165"
     CDS             2133..2555
                     /locus_tag="HV207_RS30155"
                     /old_locus_tag="HV207_30165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004207907.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAISTEVNTIGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1419..1784)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 atcgtgtgct aagtcgtccg gtgacttgcc ataacgtgat tgccggccgt cgacgaaccc
       61 gattcctgaa cgtgctgaaa accggttttc cggctgcgcc ggcaacgctt cacggccacg
      121 caataaattg cgtccccgtt caccgttttc ggatgggaag aacgccggaa acaggactta
      181 ccccggctga atggtgtgaa tttccgcgct atgcacttgc gcgcatactc atgcatgccg
      241 taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg
      301 gtttcccgtc agaaacgcgc tgagggcctc tcaggcggtg ctgataacgg atgttatggt
      361 aaatgttgga tagcaaggac tatcagtccg gcaatgctac atatcattgc taatggattt
      421 acgagcaaaa tgccgaatgt tgtcgcagat aatccgcgaa caatgatatt ggcaagaagt
      481 ccgccaacaa tgagggataa aaaggtataa ccagcaactt tcagtaacga cagatctccc
      541 catatcacct ctactatcgg atagataaaa acaccaacgc cagcaggaag cgtagtaacc
      601 atcaacagta aatggctttt agtgggaccg tactctctga catttccaac tttcatcatg
      661 tgtgtgatga atgacgatat ccaccatagg atcatgatca taaggaaatt cataattatt
      721 tactctcgat gcgtagatca ggtgctttct gtagatttgg tcgtacatac tcgctgtctc
      781 tttcgtcaag acaacggaag tagagtgtta ctgtggtatc tatccccatt cttgtgttag
      841 ccacgttttc gttagtgagc aggaattttt taccgaggct attacatttc tcagaacctg
      901 ttttaaaggc actttgacgt gctgatgccg catctctaaa accatcagca gttgctgaaa
      961 ccgtatatgt atccctacct aactcaagaa cgttgctggt agaaacacaa ccaactgtaa
     1021 gcagcgatat tgataaaata cctaacctat aaatcattga ttttattacc caagttgggt
     1081 gattgtggat catgattata tctgtttttt ttattattaa aatcaagggg ttcaataatt
     1141 ttcttcaaag aactggtgtc gtggagtggt gctggtttcc ctttgggggg gagagggatg
     1201 gaataggatc ctcttgagat cctttctgtt gcgtcgtaat ctcttgccct ggaaacgaaa
     1261 aaaccgccca gcaaggcggt tttttcgaag gttcttagag cagcaactct ttgaaccgag
     1321 gtaactggct ttcgcagagc acagaaacca aattagttat tacagtgtag ctgtacttca
     1381 cctcacactt caacatttct ttgttctgcg aatccgttac cagtggctgc tgccagtggc
     1441 gataagtcgt gtctttccgg attggactca agacgatagt taccggataa ggcgcagcag
     1501 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     1561 ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggagtga caccggtaaa
     1621 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
     1681 ttatagtcct gtcgggtttc gccaccactg atttgagcgt ccgtttctgc gatgcttgtc
     1741 aggggggcgg agcctatgga aaaacggcaa cgccgtggcc tttccttttt ttcctccttt
     1801 gaccgctcgg ttttctcgag cttttctatg catgctcctg ccgctcgccg cagtcgaacg
     1861 accggagcgt agcgactgag tgagcgagga agcggaaaag agacaggttt tacactgagc
     1921 actgacgctc tgaggccctt tatgacgaaa caacagaaaa cggcgctgaa catggctaaa
     1981 ttcatccaga atcagtctct gctcctgctg gagaagctta acgagcttga tcttgatgct
     2041 gaggccgacc tttgtgagaa actccacgat gatgcagagc atcttttccg tacactctcc
     2101 tcgagactgg attcattaca ggatggtaac tgatggccag agtgtctatt agtgaagctg
     2161 cacggcttgt taaggttagt cgtcccacta tttacaagat gattaacagc gggaagttaa
     2221 gttatacctc tgttgtaaag cacgggaagg atattaaagt tattgatgta tccgagctta
     2281 ttagggtgtt tggttctctt gatggtgtaa ctgatactgt aaaatatgat gtaaaaagtg
     2341 acgcaatttc tacagaggtt aacaccattg gtttacatga tttacaacat agaatcgctt
     2401 tacttgaagc tgaaaacgat ggtcttaaag gggctgttaa agcacgcgat gagcatattg
     2461 attcccttcg ccaggctatg cagcttttgg agcacaagca cgacccctct tcaccggctc
     2521 attctccgtg gtggaaattc tggaaaaaat cctgatcctg ccatgtttca gttttgctct
     2581 ccggcgatcc cggggagttc ttgaaccttc ccaaaaatcg ccagatctct gaacgctcct
     2641 caccactaaa aatacccaca ccccacgcaa aattaaaatt ttgcagattc cttatttaca
     2701 acaatggttt aatgaaaaat aaatgtgcgg tgatgtgtta caaagtgcag atccaagtcg
     2761 tggacgactc tatggcgaat cgctgacgaa ttgcccacga ttcatatgtg aatcacgctt
     2821 tattatcata ttgttatgat gaatcgactc ttgattcaat tcatcacttt gggtgttttt
     2881 tgttcatggt gactcttgat tcaattcacg attcgaatcg ccggtga
//
