LOCUS       NZ_CP055456             3271 bp    DNA     circular CON 01-MAY-2024
DEFINITION  Klebsiella pneumoniae strain RHBSTW-00574 plasmid pRHBSTW-00574_6,
            complete sequence.
ACCESSION   NZ_CP055456
VERSION     NZ_CP055456.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148656
            Assembly: GCF_013704985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00574
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.5756156162598x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013704985.1-RS_2024_05_01
            Annotation Date                   :: 05/01/2024 04:39:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,593
            CDSs (total)                      :: 5,469
            Genes (coding)                    :: 5,311
            CDSs (with protein)               :: 5,311
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 56 of 158
            Pseudo Genes (incomplete)         :: 93 of 158
            Pseudo Genes (internal stop)      :: 30 of 158
            Pseudo Genes (multiple problems)  :: 18 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055456.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00574"
                     /isolation_source="Freshwater sample from downstream of
                     wastewater treatment plant"
                     /db_xref="taxon:573"
                     /plasmid="pRHBSTW-00574_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2968..3271,1..98))
                     /locus_tag="HV239_RS27690"
                     /old_locus_tag="HV239_27695"
     CDS             complement(join(2968..3271,1..98))
                     /locus_tag="HV239_RS27690"
                     /old_locus_tag="HV239_27695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032744268.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFQQPKGEHHSGQVAEQRDTAFADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(98..289)
                     /locus_tag="HV239_RS27695"
                     /old_locus_tag="HV239_27700"
     CDS             complement(98..289)
                     /locus_tag="HV239_RS27695"
                     /old_locus_tag="HV239_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032744269.1"
                     /translation="MTKQQKTALNMAKFLQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1010..1489)
                     /locus_tag="HV239_RS27700"
                     /old_locus_tag="HV239_27705"
     CDS             complement(1010..1489)
                     /locus_tag="HV239_RS27700"
                     /old_locus_tag="HV239_27705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]; GO:0008080 - N-acetyltransferase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_065365727.1"
                     /translation="MANFSCGVTHLDEFLKTDLHKMQGRSLLKGYVLLSDDETPEIMGY
                     YTLSGASFERGHLSNRLRKKIPYSNMPAVLLGRLAIDQRIQRKGFGELLIVDAIHKVRA
                     TAGQIGIYAMFVEAHPGASSFYTRMGFIPSSMADDNKTFFYPANQFDDLIFGDKC"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(1531..1815)
                     /locus_tag="HV239_RS27705"
                     /old_locus_tag="HV239_27710"
     CDS             complement(1531..1815)
                     /locus_tag="HV239_RS27705"
                     /old_locus_tag="HV239_27710"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="WP_044316655.1"
                     /translation="MNAVRKERFEFRLPEAAKRRIEEAAVISKVSVSQFVMESATFRAE
                     TVINEHRRLIVEEDVWEQVMAALERPPAPTQSMCKAFERYNSEESWICD"
                     /name="DUF1778 domain-containing protein"
     Typing          338..818
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.683"
                     /coverage="92.0"
                     /evalue="1.2800000000000002e-163"
ORIGIN
        1 ctacaggtgg acaggtcacc tgctttaatt aacctgtgca gcgttgttct actcttaccc
       61 gtcagtcggg cagcctcact aatagacact ctggccatca gttaccatcc tgtaatgcat
      121 ccagtctcga ggacagtgta cggaaaagat gctccgcatc atcgtggagt ttctcacaaa
      181 ggtcggcctc tgcatcaaga tcaagctcgt taagcttctc cagcaggagc agagactgat
      241 gctggaggaa tttagccatg ttcagagctg ttttctgttg ttttgtcata agaagcctca
      301 gagcgtcagt gctcaatgta gggatgttct cttttccgct tcctcgctca ctcagtcgct
      361 acgctcctgc cgttcggctg cggcgagcgg tcgcaactca cgtataagag cataagacga
      421 cagggctgat aaccggaggg gacaagataa ggtcgcggcg ttgccgtttt tccataggct
      481 ccgcccccct gacaagcatc atagaaattg acgctcaaat cagtggtggc gaaacccgac
      541 aggactataa agatacctat catttctgat aggtggctcc ctcgtgcgct ctcctgttcc
      601 tgccattcgg tttacccgtg tcattccgct gttatggccg cgtttttctc attccacgcc
      661 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
      721 ttcagtccga ctactgcgcc ttatccggta actatcgtct tgagtccaac ccgaataggc
      781 acgacaaagc gccactggca gcagccactg gtaactgatt agaggagtta gtcttgaagt
      841 tatgcgccgg ttaaggctaa actgaaagga ctgatttggt gactagctcc tctttagcca
      901 gttaccttgg tttagtgaac ttgccggttc accaacctta aaaaagccac cttgccgggt
      961 ggtttttttt tactttcaca tatatctcat agatatatgt gaaagtactc tagcatttat
     1021 cgccaaagat aagatcatca aactgattag ctgggtagaa aaatgtttta ttatcatcag
     1081 ccattgatga tggaatgaat cccattcttg tgtaaaaaga tgaagctcca ggatgcgcct
     1141 caacaaacat ggcatagata ccaatctgtc cagcagtagc cctcacttta tggatagcat
     1201 cgacaattag taactcacca aaaccttttc gctgaattcg ttgatcgata gcaagacgac
     1261 ctaacagcac tgccggcatg ttcgagtaag gaattttctt cctcaggcgg ttactcaaat
     1321 gccctcgttc gaacgatgca ccgctcagcg tgtagtaccc cataatttct ggagtttcat
     1381 catcagaaag cagaacgtag ccctttaata ggctacgccc ctgcattttg tggagatcgg
     1441 ttttcaaaaa ctcatctaaa tgtgtgactc cacagctaaa gttcgccatg ctgtactcag
     1501 cacacaagtc aaaaggttga aaaccaggga ttaatcgcaa atccaactct cctcagagtt
     1561 ataacgctca aacgctttac acatactttg tgttggtgct ggagggcgtt caagggcagc
     1621 catgacttgc tcccaaacat cctcttcaac gatcaagcgg cgatgttcat taataactgt
     1681 ttcagctcta aaagtcgcag actccattac aaattgagat acggacactt tagaaataac
     1741 agcagcttct tcaatacggc gcttcgcagc ttcgggcaga cgaaactcga accgctcctt
     1801 tcttactgcg ttcataacaa ctcctgagct aatgctcaca gtggtttata ccacatgacg
     1861 atatgcacgg tacggtaatt taccgtacag gtaatttgag gtgattttga tctgagaaac
     1921 tatgccagtc aagctaatga agggtgaggg tagtattttt tgagaaaaaa acttaaattt
     1981 cggaaaaata accttaaaaa acaacaagtt acatatggca atgacactta gggggcattg
     2041 gtagttctta agaactacca atgcccccta agtgtcattg ccagtgtatt ttatcatttt
     2101 tttgcatgta aatctaccgc tattttgaac gaatgtatgg gccaaatcac acagcattca
     2161 ggggatgtgc attacggtat tttgcgcccc ataaatccac ccacgcactc caaaaccagc
     2221 cactgatcca gatttaccat aacgtctcat tacgcgcacc gctcaaacgc cccagaaacg
     2281 attctgggcc gtaatccatc aaaccctaca tcttaccccc tgaaaacgcc tcaaaatcgc
     2341 cccagggctt tttacgggca tgaattgtta tgcaccagac tgactggtgg cggatttcag
     2401 cctgaattca cgccagacaa ctcaaaaccc gcaccaaacc cgactgaaaa cggtgaacgg
     2461 ggacgcaata aattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa ccggttttca
     2521 gcacgttcag gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa gtcgccggac
     2581 gactcaccac acgattcacc ggcgattcga ctcgtgactt gactctagag tcgccatggt
     2641 caaaaagcca ccaacccggc aagtcgactc tagagtcaag tcatcataac gtactgatta
     2701 tatggcgtga ttcacatatg aatcgtgggc aagtcatcgg cgattcgcca tagagtcgtc
     2761 cacgactcgg attcacctta caaaaaataa aattgcgtaa tttaaaatag ataactgatt
     2821 gaaaataaag gtctatctgc aaaattttaa ttttgcgtag tgtgtgggta tttttagtgg
     2881 tgaggagcgt tcagggatct gacgattttt gggaaggttc aggaactccc cggaatggcc
     2941 ggagagcaaa atggaagcat ggcgggatca ggattttttc cagaatttcc accatggcgc
     3001 cggggattca gttaattttg cctggtgatt ttcaagaagg agcatggcct gtttcaggct
     3061 gtcaatgtgc gaatccttgg acaaaagtaa ggtgttcagg tgttcaatct cctgtttcag
     3121 atgacgaatc acctgttcac tctggtcagc aaaggctgta tcacgctgtt cagcgacctg
     3181 tccagaatgg tgttcgccct tcggttgctg gaagggacca aagacacgta aaagctctga
     3241 agtatctacc atttttgcat tgcgttcacc g
//
