LOCUS       NZ_CP055425             7945 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00862 plasmid pRHBSTW-00862_5,
            complete sequence.
ACCESSION   NZ_CP055425
VERSION     NZ_CP055425.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148721
            Assembly: GCF_013604545.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00862
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.316200522522x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 13:50:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 5,025
            CDSs (with protein)               :: 5,025
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 40 of 132
            Pseudo Genes (incomplete)         :: 88 of 132
            Pseudo Genes (internal stop)      :: 27 of 132
            Pseudo Genes (multiple problems)  :: 21 of 132
            Pseudo Genes (short protein)      :: 1 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055425.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00862"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00862_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(7502..7945,1..393)
                     /gene="repC"
                     /locus_tag="HV304_RS26270"
                     /old_locus_tag="HV304_26275"
     CDS             join(7502..7945,1..393)
                     /gene="repC"
                     /locus_tag="HV304_RS26270"
                     /old_locus_tag="HV304_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_139690569.1"
                     /translation="MTKYNLTHLRHDPSHCLAPGLFRALKRGERKKLKLDVAYDYGDGK
                     RVEFSGPEPLGADDLRVLQGLIAMAGPSGLVLGPDPKTEGGRQLRLFLEPKWEAVEADA
                     LVVKGSYRMLASEIGAGEGGEQFRIIRDVIERLWKVSIIAQNGRKRQGFRLLADYASDE
                     ADGRLFVALNPLIAQAVMGGGQHVRISMAEVRALRSDTARLLHQRLCGWIDPGKSGKAT
                     VDTLSSYVWPEEASGATMRQRRKRVRDSLPEIVSLGWTVTEYAPSKYNIARPKATA"
                     /name="repC"
     gene            complement(1353..1730)
                     /locus_tag="HV304_RS26275"
                     /old_locus_tag="HV304_26280"
     CDS             complement(1353..1730)
                     /locus_tag="HV304_RS26275"
                     /old_locus_tag="HV304_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_008786737.1"
                     /translation="MTSRKTTSADTAQCSTTSHDAATDALAMSQSDVAVLAETFRLLGD
                     QSRLKILLQCMHGSVAVGDIAEALDLSQSLVSHHLRLLRGARLVRGERQAKHIFYSIAD
                     QHVSQVLQDMAVHISEDKADD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(1774..2871)
                     /locus_tag="HV304_RS26280"
                     /old_locus_tag="HV304_26285"
     CDS             complement(1774..2871)
                     /locus_tag="HV304_RS26280"
                     /old_locus_tag="HV304_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_008786738.1"
                     /translation="MSQSHAHDHGHDHDHDHDHTPTVTSANERKVLLSFFLIFGFMVVE
                     AVGGVLSGSLALLADAGHMLTDAAALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLN
                     AVTLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLVNVLVLWIMTRGETDHVNVK
                     GAILHVMGDLLGSVGAIVAAIVIYLTGWTPIDPILSVLVAALILRSAWKLLAKSIHILL
                     EGAPEDASPEKVEQGLMGAVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKR
                     VEAVLREQFSIEHATVGIDWNSGTDENVCSLQPTSERQDHSGHDHSGHDHSGHDHSGHD
                     HGNHKHGGGHSHGHDHDHSAPGHRH"
                     /name="cation diffusion facilitator family transporter"
     gene            3096..3353
                     /locus_tag="HV304_RS26285"
                     /old_locus_tag="HV304_26290"
     CDS             3096..3353
                     /locus_tag="HV304_RS26285"
                     /old_locus_tag="HV304_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="WP_019396551.1"
                     /translation="MEDMHKHTTHPDLVKRLKRAEGHLRHVIGMIEGGETCLDIARQLA
                     AVESAVTAAKRVLIHDHIDHCLSHDEDSVLTEMKALTKLL"
                     /name="metal-sensing transcriptional repressor"
     gene            3363..>3527
                     /locus_tag="HV304_RS26290"
                     /old_locus_tag="HV304_26295"
                     /pseudo=""
     CDS             3363..>3527
                     /locus_tag="HV304_RS26290"
                     /old_locus_tag="HV304_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /name="MFS transporter"
     gene            complement(3729..3917)
                     /locus_tag="HV304_RS26615"
     CDS             complement(3729..3917)
                     /locus_tag="HV304_RS26615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259472377.1"
                     /translation="MIEAGKLGQFNEVWLRAELDKQLDRDPDRALFGLPPLAKAKPEQV
                     VQEEAPAGLHTAPTAAQ"
                     /name="hypothetical protein"
     gene            4243..6351
                     /locus_tag="HV304_RS26300"
                     /old_locus_tag="HV304_26305"
     CDS             4243..6351
                     /locus_tag="HV304_RS26300"
                     /old_locus_tag="HV304_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_139690572.1"
                     /translation="MAIFHLTAKTGSRGGGQSAKAKADYIQREGKYARDKDEVLHTQSG
                     NMPSWAAGAAEVSNYWDAADLYERANGRLFKEVEFALPIELTLDQQRVLVDDFAKYLTE
                     VERLPYTLAIHKGGDENPHCHLMMSERKNDGVERKKDQWFSRYNPKKPGQGGAQKTESL
                     KPKAWLEKTRAAWADHANRALEMAGHEARIDHRTLEAQGIERLPTIHLGPNVVEMEGRG
                     IRTGRADTALSIETANTEIIDLQAYRESIDHERDRQSEAGQRDRRAGGADRAAGAELGD
                     SRRQGRDGDRRDSKCKHKAGWRVEDPAGQSREGVGRHSQGHEGRSRGTGHGSREGRGRS
                     ESVGMEDFRSHLDGFSAVYASAADRIVALAVPGTQDDKGGGSVATTQAQVKGDRTSQAI
                     ARQLKAMGCDLYDIGIRDSATGKMMNREWTLQEVVENAAWLKRMNAQGNDIYIRPAESA
                     RHGLVLVDDLSSFDLDAMKEEGREPAAVIETSPKNYQAWVKVAANAPAEHRGAIARRLA
                     REYDADPASADSHHYGRLAGFTNQKPKHTTKTGYQPWVLCRASSGQAATTGPQMVQEAG
                     QELESKERQQEQARRLEGIETAQPRRYHRTALDEYRSEMAGLVKRFGDDLSKCDYIAAM
                     KLAMKGIDPDEIAKAMAEASPGLMDRKAGHEADYIERTIKKVMALPEVQKIRAELEQTQ
                     KPSGYTPR"
                     /name="DNA-primase RepB domain-containing protein"
     gene            6436..6642
                     /gene="urfF"
                     /locus_tag="HV304_RS26305"
                     /old_locus_tag="HV304_26310"
     CDS             6436..6642
                     /gene="urfF"
                     /locus_tag="HV304_RS26305"
                     /old_locus_tag="HV304_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_139690571.1"
                     /translation="MKDKRDKLTGNLLASDNAIRQARFAETQRELGRKARKIWATDDEA
                     ETLRLYLDKIRAAQDGDRDPASA"
                     /name="urfF"
     gene            6673..7515
                     /locus_tag="HV304_RS26310"
                     /old_locus_tag="HV304_26315"
     CDS             6673..7515
                     /locus_tag="HV304_RS26310"
                     /old_locus_tag="HV304_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_139690570.1"
                     /translation="MATHNPIDILAAFTAPPPPIDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAMQIAGGPDLLGVGELPTGRVIYLPAEDPPVAIHHRLHALGAHLSAAERQAVAT
                     DLWIKPLIGNGPNIMTPEWFDGMMKAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAAETGCSIIFLHHANKSAAMAGAGDQQQASRGSSVLVDNIRWQAYLSGMTAAEAET
                     WGVDDNQRAYFVRFGVSKANYGSPFQERWLRRHEGGVLKPVPLERQAAKAKGTQRDKI"
                     /name="helicase RepA family protein"
     Typing          6674..7471
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          4244..6345
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.86"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaacggcagg gcttcaggct gctggcagac tacgccagcg acgaagcaga cgggcggctc
       61 ttcgtggccc tgaacccctt gattgctcag gccgtcatgg ggggcggtca acatgttcgc
      121 atcagcatgg ccgaagtccg ggcgttgcgg agcgataccg ctcgcctctt gcaccaacgg
      181 ctatgcggct ggatcgaccc cggcaagagc ggcaaggcca ctgtggatac cttgtccagc
      241 tacgtttggc ccgaggaagc cagcggggca accatgcgcc aaaggcgcaa aagggtacgg
      301 gattcgctgc cagagattgt ctcattgggc tggacggtca ccgaatatgc ccctagcaag
      361 tacaacatcg cacggcccaa ggcgaccgca tgacccctgt agtaaccgtc acgcacccct
      421 gtagtaaccg tcacgcaccc ctgtagtaac cgtcacgcac ccctgtagta accgtcacgc
      481 tcaggggagt aaaaacaacg ctgtaagcca cgccaccact ggcctgtagc atttgcgcaa
      541 aaagttcatc caagatcttc caagatcttc cataggatct gagcccttta ggggctccga
      601 ccctctacag attacagacc tacagaccaa tgcccggcca gaggccggag gccctttatc
      661 gctgcgctaa gctacacacc cccccccact gctgcgcagt agggggaaag gcgggcttcg
      721 cccgctggtg cctgtctgag acgccacagg aggcccgtag aggggttcta gggattttcc
      781 gtccaaaaac gacaaagtgc tctggaggcc acgccgtccg cggcctccag aggggtatta
      841 cttttttggc gcttcacata gccagtcaca ataaatcaat cacttagcag tctgaatgtc
      901 gcatttggac ggaaaatccc tagaaccccg aagtgcccct ttcttggatg caggactccg
      961 gcccttccga agcgctctgc agaaatttgt gtggaggcta ggcgttacaa cgaaggcaag
     1021 gtcaagccct ttgggttgcc ctagcgggca ggctgcatgg acaaggtggt gggaaaaccc
     1081 cgcgtcgtcg ctgtggccac tccgacatcc agctcacgag tgccgttgtg cggttgaacc
     1141 ggtcggtctt gtgggtgatt gcgagcgcgg gaggctcagg cgtccaagct gccgccgccc
     1201 attgctgtgc ctgcgcggca tcccggcgcc caggcagggc ccgcgattca gggtgttgcg
     1261 cccaggccgc atcgaggacg tccttgaggc taaccggcgg cgtgggtacc ggcgcctcgc
     1321 aacctgccat tcgcctgggt gcttgggcct ggtcagtcgt cggctttgtc ctcggaaatg
     1381 tggaccgcca tgtcctgcag cacctggctg acgtgctggt ccgcgatgct gtagaagatg
     1441 tgcttggctt ggcgttcgcc gcgcacgagg cgcgcaccgc gcagcagccg caggtgatgg
     1501 ctgaccagag actgcgacag gtcgagcgct tcggcgatgt cccccacggc gaccgagcca
     1561 tgcatgcact gcagcaggat cttcaatcgc gattggtcgc ccagcaggcg gaaggtctcg
     1621 gccagaacag cgacgtcact ttgcgacatt gcgagggcat cggtcgccgc gtcgtggctg
     1681 gtggtcgagc attgcgcggt gtcggcgctg gtggttttgc gggacgtcat ggatgcaaat
     1741 cctcaagtgg agtgcgagcc ggctcgcgcg gcctcaatgc ctgtgaccag gagcggaatg
     1801 gtcgtggtca tggccgtggc tgtgcccgcc gccatgcttg tgattgccat gatcatggcc
     1861 gctgtggtcg tgcccgctgt ggtcatgacc gctgtgatcg tggccgctgt ggtcctgacg
     1921 ctccgacgtg ggctgcaagc tgcagacgtt ctcgtcggtg cccgagttcc agtcgatccc
     1981 gaccgtggcg tgttcgatgc tgaattgctc tcgaagcacc gcttcgacgc gcttgaccac
     2041 ggcgcgtgct tcctcgtcca cgttggggcg aacgtgcagc gtggccatcg tgcgcccgga
     2101 ggtgagctgc cacacgtgaa cgtggctgac ggccgccagg cccggcacgg cgcccatcag
     2161 gccttgctcc accttctccg gcgaagcatc ctccggcgcg ccttccagca ggatgtggat
     2221 cgacttggcg agcagcttcc atgcgctgcg caggatcagc gccgcgacca ggaccgacag
     2281 gatgggatcg atcggcgtcc agcccgtgag gtagatgacg atcgcagcga cgatggcgcc
     2341 gaccgagccg agcaggtcgc ccatcacgtg caggatggcc cccttcacgt tgacgtgatc
     2401 ggtctctccg cgggtcatga tccacagcac gagcacgttc accagcaggc cgagcacggc
     2461 gacgatcatc atcgggccgg ccaggatcac gggcggcgcc tgcagccgct cccacgcctc
     2521 gtaggcgatc caagcgacga tcgcgaacag ggtcacggcg ttcaggaaac cggcgatgac
     2581 ctcgaagcgc aggtagccga aggtgcgctt gctgtcggcc gcgcggcggc cgaaccggaa
     2641 tgctgcgtag gccagtgcga gcgccgcggc gtcggtcagc atgtggcccg cgtcggccag
     2701 caaggccagc gagcccgaca gcacgcctcc gacggcctcc acgaccatga agccgaagat
     2761 caggaaaaag gacagcagga ccttgcgctc gttggcgctg gtcacggtcg gggtgtggtc
     2821 atgatcatgg tcgtgatcgt gaccatggtc atgtgcgtgg gattgggaca tggggcctcg
     2881 ggaaactggg tctttgactg tggctcaaaa tataacacat gaacatgttc tcatgtgaat
     2941 tgttgtacac atttgtgttc gcgtctcacc acggccggga tgccgctccg ggcaattgcc
     3001 ttagcctggc tcggcgacca ttccccccgc ctggcaagca gccgttgatc taacatccag
     3061 tggcagtatc ccccctgggg ggatatgatt tcagcatgga agacatgcat aagcacacca
     3121 cccatcccga cctggtgaag cggctcaagc gcgccgaggg gcatctgcgg cacgtcatcg
     3181 ggatgatcga agggggagaa acctgcctcg acatcgcccg ccagctcgcg gcggtggaaa
     3241 gcgcggtgac ggcggccaag cgcgtcctga tccacgacca catcgaccac tgcctgtccc
     3301 atgacgagga ctccgttctg accgaaatga aggcgctgac caaactgctc tgaggccgtc
     3361 cgatgctctc cgtcctgaat aaccgcacct accgccacct gttcaccgcc caggtgatcg
     3421 cgctcgtcgg caccggcctg atgacggtgg cgctcggcct gctggcctac gagctggccg
     3481 gcgcggatgc aggcgcggtg ctcgggacgg cgctggccat caagatgggg ttctagggat
     3541 tttccgtcca aaaacgacaa agtgctctgg aggccacgcc gtccgcggcc tccagagggg
     3601 tattactttt ttggcgcttc acatagccag tcacaataaa tcaatcactt agcagtctga
     3661 atgtcgcatt tggacggaaa atccctagaa ccccgtagag ctgttttgcc gcgacagcgg
     3721 cctttcccct actgcgcagc agtgggggcg gtgtgtagcc ccgcaggggc ctcttcctgc
     3781 accacctgct ctggcttagc ctttgccagt ggtggcagac caaacagggc tcggtctgga
     3841 tcacggtcaa gctgcttgtc cagctcagcc cgtagccaga cctcattgaa ctgccccagc
     3901 tttcccgcct ctatcatgcc cgtgagcatt gccccaacta tgatcttgcg ccgagtatca
     3961 tcccgccgtt cctgctgctg ttcccttgcc ctcactcgct ggatctcggc attgatccgc
     4021 gccctctgtt cttcgagctt ggcgagttta tcctctaact ttcccatgtt gacagccctg
     4081 ttgttatgtg cccgttgtag agtatcatca ggttactgat gccgcaaacc cgactcatgg
     4141 aacatgaaga gggcgcactt accggtttct ctgcgagaaa ctgccttcgg ccccccttcg
     4201 gggcggtgcg ctctccgagg gccactagcg tggagcttac acatggcgat atttcatctc
     4261 acggccaaga ctggaagccg tggcggcggc cagtcggcta aggccaaggc cgactacatc
     4321 cagcgcgagg gcaagtatgc ccgcgacaaa gacgaggtgc tgcacaccca gtctggcaac
     4381 atgccctcat gggcggcagg agccgcagag gtcagcaact actgggatgc cgccgacctg
     4441 tacgaacggg ccaatggccg cctattcaaa gaggttgaat ttgccctgcc aatcgagctg
     4501 acgctcgatc agcagcgggt gctcgtggac gacttcgcca agtacctgac cgaggtcgag
     4561 cgcctgccgt atacgctggc gattcataag gggggggatg agaacccgca ttgccacctg
     4621 atgatgtccg agcgcaaaaa cgatggcgtc gagcgcaaga aagatcagtg gttcagcagg
     4681 tacaacccga aaaagccggg gcaggggggg gcgcagaaaa ccgagagttt gaagcccaag
     4741 gcgtggcttg agaagacccg agcagcatgg gcagaccatg ccaaccgggc gctggagatg
     4801 gcaggccacg aagcccgcat cgaccaccgg acgctagagg cgcaggggat cgagcgcctg
     4861 ccgaccatcc accttggccc caacgtggtg gagatggagg ggcggggcat ccgcaccggg
     4921 cgggcggata cggccctgtc catcgagacg gccaacaccg agatcatcga cctacaggca
     4981 tacagagaga gcatcgacca tgagcgcgat agacaaagtg aggcaggcca gagggatcga
     5041 cgagctggcg gggcagatcg agccgctggc gcagagcttg gcgactctcg cagacaaggc
     5101 cgtgacggcg atagaagaga ctcaaaatgc aagcataaag caggctggag agtggaagat
     5161 ccagcagggc aaagccgcga aggcgtggga aggcacagcc aaggacatga aggacgcagc
     5221 cgaggcactg gacacggcag ccgggaaggc cgagggcgca gcgagtcagt tgggatggag
     5281 gattttcgta gtcacctcga tgggttttct gctgtttatg ctagtgctgc tgatcggatt
     5341 gtggctctgg ctgtccccgg aactcaggac gacaaaggag ggggcagtgt ggctactact
     5401 caggctcaag tgaaggggga tcgcacctct caggccatcg cccgccagct caaggctatg
     5461 ggctgcgacc tgtacgatat tggcatcagg gactccgcca caggcaagat gatgaatcgg
     5521 gaatggacgc tgcaagaggt ggtcgagaac gccgcatggc tcaagcggat gaacgctcag
     5581 ggcaacgata tctatatccg gccagccgaa tcagccaggc acgggcttgt cctggtagac
     5641 gacttgagca gctttgacct ggacgcgatg aaggaggaag ggcgggagcc tgccgccgtt
     5701 atcgagacca gcccgaagaa ctatcaggcg tgggtgaagg tagcggcaaa cgcgccagca
     5761 gagcacaggg gcgcgattgc ccgcaggctg gcccgagagt atgacgccga ccccgcaagc
     5821 gctgatagcc accactatgg ccgtctggcg ggctttacca accagaagcc caagcacaca
     5881 accaagacgg ggtatcagcc gtgggtgctg tgccgagcgt ccagcggcca agccgccacc
     5941 acaggccccc agatggtgca ggaggccggg caggagcttg agagcaagga gcgccagcag
     6001 gagcaggccc gcaggctgga gggcatagag acagcccagc cgcgccgtta tcacaggact
     6061 gccttggacg agtaccgcag cgaaatggcg gggctggtga agcggttcgg ggacgacctg
     6121 agcaagtgcg attacatcgc cgccatgaag ttggccatga agggcataga tcccgacgag
     6181 atcgccaagg ccatggccga ggccagcccc ggcctcatgg atcgcaaggc aggccatgag
     6241 gccgactaca tcgagcgcac catcaaaaaa gtgatggcgc tgccggaggt gcagaagatc
     6301 cgcgccgagc ttgagcagac gcaaaaacca agcgggtata ccccacgctg aaaatgtgac
     6361 ttttagttag agctaaccca tgttatgctg ttagctctaa ccgtgtattt tgcattagct
     6421 ctaatgaggt ctggtatgaa ggacaagcgg gataaattga ctggcaacct gttggccagc
     6481 gataacgcga tcaggcaggc tcggtttgcc gagacccagc gtgaactggg gcgcaaggcg
     6541 cgtaagatat gggccacaga cgatgaggcc gagaccttga ggctttatct ggacaagatc
     6601 agagcggcgc aggacgggga tcgcgacccc gccagcgcct aaccacagac cgcctaaaca
     6661 ggaggcaaat caatggctac ccacaatcct atcgacatac tggcggcttt cacagcccca
     6721 ccccctccaa ttgattatgt gttgcccaac atggtggccg gcaccgtggg ggcgctggta
     6781 tcccccggtg gggctggcaa gtccatgctg gccctgcaac tggcgatgca gatcgctggc
     6841 ggccccgatc tgctgggcgt gggcgagctg ccgacaggcc gagtgatcta cctgcccgca
     6901 gaagacccac cagttgccat ccatcaccgc ctgcacgcac tcggggcaca cctctccgca
     6961 gccgaacggc aggccgtggc aaccgacttg tggatcaaac cgctgatcgg aaacggcccc
     7021 aacatcatga ccccggagtg gttcgacggc atgatgaaag ccgccgaggg cagacgcctg
     7081 atggtgctgg acacgctcag gcgcttccac atcgaggagg agaacgcctc cgggcctatg
     7141 gcccaagtca tcggccgcat ggaggccatc gccgccgaga cagggtgctc gatcatcttc
     7201 ctgcaccatg ccaacaaaag cgcggccatg gcgggcgcag gcgaccagca gcaggccagc
     7261 cgtggatcgt cggtgctggt cgataacatc cggtggcagg catacctctc cggtatgacc
     7321 gcagccgagg ccgaaacgtg gggcgtggac gacaaccaga gggcctattt cgtgcgcttt
     7381 ggcgtgagca aggccaacta cggcagcccg tttcaagagc ggtggctaag gcggcacgag
     7441 ggcggcgtac tcaagcccgt accgctggaa aggcaagcag ctaaggccaa ggggacgcaa
     7501 cgtgacaaaa tataacttga cgcacctccg gcatgacccg tcgcactgtc tggcccccgg
     7561 cctgttccgt gccctcaagc ggggcgagcg taagaagctc aagctcgatg tggcctatga
     7621 ctacggggat ggcaagcggg tagaatttag cggcccggag ccactgggcg cagacgacct
     7681 tcgcgtcctg caaggtctga tagctatggc agggccgagc ggtctggtgc ttggcccaga
     7741 ccccaagacc gaaggcgggc gacagctccg cctgttccta gagccgaaat gggaggccgt
     7801 tgaagctgac gcactggtag tcaaaggaag ctatagaatg ctcgccagtg aaatcggtgc
     7861 gggagaaggt ggcgagcagt tcaggatcat cagggacgtg atagagcgac tgtggaaagt
     7921 ctcgatcatt gctcagaatg gacgc
//
