LOCUS       NZ_CP055424            12871 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain RHBSTW-00862 plasmid pRHBSTW-00862_4,
            complete sequence.
ACCESSION   NZ_CP055424
VERSION     NZ_CP055424.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148721
            Assembly: GCF_013604545.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 12871)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00862
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.316200522522x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 13:50:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 5,025
            CDSs (with protein)               :: 5,025
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 40 of 132
            Pseudo Genes (incomplete)         :: 88 of 132
            Pseudo Genes (internal stop)      :: 27 of 132
            Pseudo Genes (multiple problems)  :: 21 of 132
            Pseudo Genes (short protein)      :: 1 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055424.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00862"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:546"
                     /plasmid="pRHBSTW-00862_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(12387..12871,1..175)
                     /locus_tag="HV304_RS26190"
                     /old_locus_tag="HV304_26195"
     CDS             join(12387..12871,1..175)
                     /locus_tag="HV304_RS26190"
                     /old_locus_tag="HV304_26195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548402.1"
                     /translation="MTEIKKESADGAWLRSTLAELAGDAFPVYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            358..747
                     /locus_tag="HV304_RS26195"
                     /old_locus_tag="HV304_26200"
     CDS             358..747
                     /locus_tag="HV304_RS26195"
                     /old_locus_tag="HV304_26200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015768.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            740..1102
                     /locus_tag="HV304_RS26200"
                     /old_locus_tag="HV304_26205"
     CDS             740..1102
                     /locus_tag="HV304_RS26200"
                     /old_locus_tag="HV304_26205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061301020.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPFLHHHPGDRPAT
                     EKRAIARFKDSLAAGGQTLAFVRSDASLYALGLRFRRERTRELVASDPDVAAAGEALEQ
                     RANGVNLSYTFGGGDE"
                     /name="hypothetical protein"
     gene            1095..1280
                     /locus_tag="HV304_RS26205"
                     /old_locus_tag="HV304_26210"
     CDS             1095..1280
                     /locus_tag="HV304_RS26205"
                     /old_locus_tag="HV304_26210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151322523.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRRELPRSTGR"
                     /name="hypothetical protein"
     gene            complement(1300..1941)
                     /locus_tag="HV304_RS26210"
                     /old_locus_tag="HV304_26215"
     CDS             complement(1300..1941)
                     /locus_tag="HV304_RS26210"
                     /old_locus_tag="HV304_26215"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_181213544.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKATKSEPQPAERTTSKAKVQPK
                     VKTPQKPAQEPTAAKAKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQC
                     LIDQCMRYDLDGNPTVAVTPEEAEQAKTRLTEIREFRRKARQEKDKKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(1938..3227)
                     /locus_tag="HV304_RS26215"
                     /old_locus_tag="HV304_26220"
     CDS             complement(1938..3227)
                     /locus_tag="HV304_RS26215"
                     /old_locus_tag="HV304_26220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181213543.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            3583..3792
                     /locus_tag="HV304_RS26220"
                     /old_locus_tag="HV304_26225"
     CDS             3583..3792
                     /locus_tag="HV304_RS26220"
                     /old_locus_tag="HV304_26225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
     gene            3779..4951
                     /locus_tag="HV304_RS26225"
                     /old_locus_tag="HV304_26230"
     CDS             3779..4951
                     /locus_tag="HV304_RS26225"
                     /old_locus_tag="HV304_26230"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172743605.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFDPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGSQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVEVMLVPDAGEAYRLAHKAAYGDDREPVPTI
                     PGASVVSFDGLNRFEPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERG"
                     /name="hypothetical protein"
     gene            5208..5654
                     /locus_tag="HV304_RS26230"
                     /old_locus_tag="HV304_26235"
     CDS             5208..5654
                     /locus_tag="HV304_RS26230"
                     /old_locus_tag="HV304_26235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548408.1"
                     /translation="MKSKTELRAAATRRALEVIASEMGGWSLDGFAHGSLPNFSPLPSQ
                     QTQEGSVVLARSPFECSWAGTAVFTAQASRFVNADPASAHYEWACGVEREAVATALMVE
                     AFNVTGCVEYAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAA"
                     /name="hypothetical protein"
     gene            complement(6022..6513)
                     /locus_tag="HV304_RS26235"
                     /old_locus_tag="HV304_26240"
     CDS             complement(6022..6513)
                     /locus_tag="HV304_RS26235"
                     /old_locus_tag="HV304_26240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548409.1"
                     /translation="MAIGDDAIRDAFYIFTQQAAEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDLPTSPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAARRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCRAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(6583..7062)
                     /locus_tag="HV304_RS26240"
                     /old_locus_tag="HV304_26245"
     CDS             complement(6583..7062)
                     /locus_tag="HV304_RS26240"
                     /old_locus_tag="HV304_26245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548410.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKNARQFAQQTARQSARQIVPRGADPRFQAVSD
                     EADQLIVSMTGENATLPAIASALNAAGYRTATGQEWNTSRVRDYRHKLRESGLLC"
                     /name="hypothetical protein"
     gene            7608..9131
                     /locus_tag="HV304_RS26245"
                     /old_locus_tag="HV304_26250"
     CDS             7608..9131
                     /locus_tag="HV304_RS26245"
                     /old_locus_tag="HV304_26250"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_061548411.1"
                     /translation="MPNHDDNSGAAVATVTQIAAAKAKAAAKTERPAFMGGSLPPVPFN
                     EWDGLTGSPTRDGFRIQAAEKRSVNFGNEYPLRYVPPLITDAGSGRWDVLNHADNLRWL
                     LYRNGARMELNLMTFKNELSWADSGSLVEPDEHGARAWILNKSAEVGLKRQAVDDFMFY
                     VARSAPYHPVARLLGSREWDGSPRVDAVLDCVPVAPGKEALRSALLRGLLIAAITAVEE
                     GAVSNKYVPCLYSEANNWFKTAFFRALFDLLPGAFGQGVSINPSSKDSVRPAVMRWFNE
                     LGEVSGMMKKNDPDEIKAFVERNVDEWRPENEKAVIAKKRQMVMGATVNKRDYLKDETA
                     ASRFPTITLVAPIDIARVNELLGWEWDNVKGCAVRADEDQLIQFWLEVRHLYRSGVGFN
                     LDDETFAAAKAETAQFVDRGSYYEPVRDILLLPGNASTGQYPWDKPGYDGRFTATDVTA
                     ALRAPSSHSGRVGRALKRLADEGIVDRRNHRGTFVYWKLPDEPTGELPL"
                     /name="VapE domain-containing protein"
     gene            complement(9818..10285)
                     /locus_tag="HV304_RS26250"
                     /old_locus_tag="HV304_26255"
     CDS             complement(9818..10285)
                     /locus_tag="HV304_RS26250"
                     /old_locus_tag="HV304_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_061548400.1"
                     /translation="MKKYTPEQKAQALKLLEQDGATAATVARTMGIPPRTVRDWAKARA
                     DAPSNVLSIEEMRERAQRAVEATPQAAVRRLKNHFVQRQYELLQRHASDLQALRSASLQ
                     AMLEKDATMVKAISGLMTSLLKAQERERLIYEIKPGTEADIMRQGMNRQQS"
                     /name="transposase"
     gene            complement(10520..10732)
                     /locus_tag="HV304_RS26255"
                     /old_locus_tag="HV304_26260"
     CDS             complement(10520..10732)
                     /locus_tag="HV304_RS26255"
                     /old_locus_tag="HV304_26260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_147191448.1"
                     /translation="MTYRLSPRLKALARWTKLANNGARWALLNTRDEVVAWGRYLSDYT
                     LCSASRRTDVRLVNVAEEIDRLIEN"
                     /name="hypothetical protein"
     gene            complement(10962..11549)
                     /locus_tag="HV304_RS26260"
                     /old_locus_tag="HV304_26265"
     CDS             complement(10962..11549)
                     /locus_tag="HV304_RS26260"
                     /old_locus_tag="HV304_26265"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181213545.1"
                     /translation="MNKTYGYTRVSTREQTADGQRDAIAAHYPDALVLGEEPMSGTVPP
                     RERPTLGILLNQILQEGDTLVVAAIDRIGRNARDTLELVEEMEKRKINLISLREGVELN
                     TATGKSVIGVVASFAEMERTLILERQAAGIAAAKKRGQKFGRPTKIGATEVGEIVTRRA
                     MGVPIASIAKQLKLSRSAIYRCLEKIDAKAPE"
                     /name="recombinase family protein"
     gene            complement(11561..12136)
                     /locus_tag="HV304_RS26265"
                     /old_locus_tag="HV304_26270"
     CDS             complement(11561..12136)
                     /locus_tag="HV304_RS26265"
                     /old_locus_tag="HV304_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151785.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_101998997.1"
                     /translation="MSRVFGYGRVSTGEQTVAGQKKAILDRYPQATFHADEGISGTVPA
                     TQRDKFKAILEFAAPGDSIVVVAIDRIGRNARSVMEVCEELEKRQVSLISLREGFDFST
                     PTGKMMLTMVAAFAEMELATLKERTKAGMAAAAAAGRHAGRPTKQTPENVGAVIMKHDA
                     GVSAARIARDLGISRGTVNRILQKHAKA"
                     /name="recombinase family protein"
ORIGIN
        1 tatcgagctg cttctcaagc tggcgatgga cgaggcgacg acgctcgctg aaaccgacgg
       61 cgggcgcggc ctgtaccgta ccgtgatcgc gcctgaccgc cgtcttaacc aaattttcgc
      121 cacgcagggc gctattgaaa tcctgcgccg taaccgtggc atggagggtg tgtaatgtct
      181 gctttcaaac tcctgtgtga ccggcttcgc aatgaggcga cgctggcaaa atctgacggg
      241 gccggcgctt tcaaaaagca atctctggat cccgacctga agggcggcaa gaagggcaaa
      301 aagcccgctg gggagctgga ggaggacgag gcgatcgcgt ctctccaccg taagcgcatg
      361 aacgacgtta aaggttgcaa gacgctgacc aaatccaccg gcgctgatga ctttggcctc
      421 cctccgctgt cgtcggtggt cgagacagtg gtggagacag gcggcgcagc gcagacgttc
      481 gcgccgatcg gggctccggt ggcgtctccg accgaagcgg ttcaccgtgc gcgtgcgcag
      541 tccatccagg acgcggcgtt cctgaacgag atgaaagcgg cgggtgtggc accggctgac
      601 ctggtgttac ttgacggcgt gttgacgtcc tctcgtggga tgccagacgc ggcgcgtgct
      661 ccggcgtttg ccgaggtgcg ccgtcaggtt caggcgggcg gcatgacgct gtcaccggct
      721 gcgcgtggta ttgactggaa tgcgtaagct gccgcgtcac ctgcagggcg aggtgttgat
      781 gccgctgtcc agcaggatca cgcgctcgca acggctggag ctgatctggc tgttgccgtt
      841 cctgcatcac catccgggcg atcgtcctgc caccgagaag cgggctattg cccggtttaa
      901 agactcgctc gctgcgggtg gccagacgct ggctttcgtc cgttcggatg cgtcgctgta
      961 tgcgctgggg ctgcggttcc ggcgtgagcg cacccgggag ctggtcgctt ctgatcctga
     1021 tgttgctgcg gctggcgagg cgctggagca gcgtgcaaac ggtgtgaacc tgtcctatac
     1081 gttcgggggt ggcgatgagt gaatttgctg tctcgcgcct ggtcattcct gaggtggtga
     1141 tccccgatgg ggtcattgct gcccgacggg caaggggaca ggctcaacgt gtcgcagctg
     1201 ctcgccgtgc tgcagagcgt cgtgctgcgc ctacgtggcg ctgggtgaga cgggaactgc
     1261 cccggtcgac agggcggtga aacggtgaga cggggcgcgt tatgcgccct tttttttgtc
     1321 cttctcctgg cgtgccttgc gtcggaattc ccggatctct gtcaggcggg ttttggcctg
     1381 ttcggcctcc tctggcgtga cggcgacggt ggggttgccg tcgaggtcgt agcgcatgca
     1441 ctggtctatg aggcactgct ggtacgagct ggtacccgtg acaatgccca ggatgcgacg
     1501 gacggctttg cggtctgcct ctggcggcat gtccagctct gcgcctcggg tcaggatctg
     1561 ctcgatgatg ccctttgcca tggggcgacg gtcaactccc cgggtcagca agtcaggcca
     1621 gtgctctttc aggaatgctt tggtcacccg gttggccagc gcctttttgg acttgttggc
     1681 gatcgctatg tcctgctgtc ggcggtcgtt ctcctcctgt ctgcggatcc cctcggcgac
     1741 ctgttcaggc gtctttttcg cctttgccgc tgtgggctcc tgtgcgggct tctgcggggt
     1801 tttcaccttt ggctgtactt tcgccttgct ggtagtccgt tcggctggct gtggctcaga
     1861 tttcgtggcc ttttccggcg ggacgtagac cgtgcggttg cgtcgcttga tggtcaggat
     1921 cggacgggtg gtttcggtca taaatctctc tctggtcggt tggtctggat gtggtcggca
     1981 agggcgatca gggtggcggc gtcgccgcgg tcccgatcga tgcgccggat gatgtcgttg
     2041 atgtagtccc agcgcccgac ggcggcaagg acggaaaggg tgaggcggtt gaaccgactc
     2101 tctggtaccc ggtcgagggt cagggggatg cgcttgcggg ggcgtcctgg accgcgccct
     2161 gtccctggtc ttttgagcag ctgcggctgc tccctctgcg gcaaaataac ggggatcttc
     2221 gccaggctgc gcgtctcgtt ctgcgcgaac tcgggcaccg cgatgtgctc gtctgtccat
     2281 gtcctgcctc cgggcctggc tgatgcgctg gagtcgcagg acgggcagga gacgacgatc
     2341 tcccgtcgcg gcttgccctt gaccggtgcc ttgctgatca ccaggggcgc ggctttgcaa
     2401 tcggagcaga ccgacgctgt ccagaccgcg ttgtctggca gggtggcgtt ccggtactgg
     2461 tgcgcggcgt catgcggtgt ctggaagctg accgtctgaa acgtgggggc gacgctgagg
     2521 ggtgccgccc atggctggca cctgggacaa gcaagctcca cctctccggc gtgcccgtcg
     2581 ccaccgttcc agcgcagggg ctcgcggtgt tcggggcagt agtacacgaa accgtcgcgg
     2641 cgtgtttgcc cgaccgggcc tggtcggcga tcatggaacc gtgcgcccgg gttggctcgc
     2701 atggcgcgga cgagcgcctc gccctggtgg atgccgtacc agacctgcca gccatcgccg
     2761 atgccgatca ggaacggcga gacgaacggt ggggtgcggt tgactctccc gcgtgcgttg
     2821 gctccgatgg tggcttcgcg ttcgcggttc cagcaggaga cgcacaggct tgaaccgggc
     2881 ttattggggg cgatcaggcg gttgtctttc cggctgcaac gggggcagag cttacgggtg
     2941 tctgccgggg tggtcagtgg tgcgtcaggg ggctgttcgg taccgaccca aagcgcgagg
     3001 ccagtggggc accggtggca ctcgaggtgg gggttttcaa tcgtgcgatc gaggtggctc
     3061 tgctcataaa tctgtcggca tgcctcgacc cggagggagc acccgccggg gcaggtgacg
     3121 aacaaaacgc cggggagctg ctcggggaat ttttcagtcg cgcttacgcg ttcaagtgct
     3181 gccgggttcg gctgcgtcga ttcgttcgtg tcgttatcgt cgttcactgc tccccctgcg
     3241 tcgaaatggt cgccagacag cgccagcact ggctatgcgt cttttttagt atctcacaaa
     3301 aaacgccact ttggcgttaa ttgtgttacg cttgtcttac tgattcctga cgagcagttg
     3361 ggaaacatga aaccggatcg cccggtgccg ctttccctcc tccccaacga ggcggggttg
     3421 taagcgagga tgcgcgcttc gtccttggca ggtgcgagta agcgagaagg gctgctgtcc
     3481 tgccgctatg cggtaagtcc tcaagggcgg ctttatcgat aatcgtcact cccgcccccc
     3541 gatgcccgtt aagctgaggg gggcacctat ctggtactcc caatgaaaaa acgaatctat
     3601 accgctgacc tcgctgcgct gctgaacgtg tcgctgcgca cgctctcgcg gctaagttcg
     3661 cgggggatcg tgccgactcc ggcagggcgc tccctccgtg gcgggcatta ctggctggct
     3721 ggcaatcccg agatcaaacg cttcgttgcc gaccagcgtc gtccggggag ggctgctgat
     3781 ggcgttcgtt gatttcgaca ctacccatct gaaagaggat ccgggcctgt tctggcgtgc
     3841 cgtatcggct gcgggtgctc cggctgatcg agacgaatgg gtgcgtctct gcctggctct
     3901 tggttgctgg gcgtctctct gcggcgagga cgggctggca gctatcgagg cctggtccac
     3961 tcgacatggg cgaggcgaga atcgccatcg ccagcagtac aggtcgtttt taaaaggcga
     4021 cctggataca ttcgccgctc aggctttcct caatttctcc ggcgtcgaat ggcgcaacgg
     4081 tcgcgcttct gtccagtccg acgtcgatcg cgctgaacgg ctgcggttgt cccgtgaggc
     4141 cacagcgaag gcaaaggctg aacgcgacaa gaagggtgat gctgctgcgc gtgctgcggt
     4201 caaactgctg gcgaagggag gggatcctgc gtctacccca tactggcgca aaaagaccgc
     4261 tctggcggct gcgggtggcg tccagctgcc cgccctcagc acagatcttg gtgttggcgg
     4321 gatgtttcga tcgttcgatc cgcgtgctta ctggtctgat tgcctcctga ttccggttta
     4381 caagttcgac gccgcccgtc agctgtatgt tgacggcctg gaggccatct gcgggcgctg
     4441 ctcggctgag ggttcccagc tcactggctc gaagttctct ccggcgggta cccgcaagca
     4501 gggcggcttc tacccgatgc cggggttcat ggacgtgctc gacggctccc cgcacgcgcc
     4561 ctactcgcct ctgttcgtct gtgaggggtt cgctacggcg ttcgccctgc accacctgag
     4621 ccgtggcaag gtggcggtct gcgctgcgtt ctcgatcgcc gggttcggca gctgcgtggc
     4681 gtcgctgcgt cggttctggc ctgatgttga agtgatgctg gtaccggacg cgggcgaggc
     4741 gtaccggctg gcgcataaag cggcctatgg agacgatcgc gagccggtcc cgacaattcc
     4801 aggggcgtcg gtggtgtcgt tcgacggcct gaaccgcttc gagccccgcg ataacctcga
     4861 ctggttcgac gtgctggttg aggagggcga ggatcgggcg cgtgagctgc tccggtatgc
     4921 ggtgcgtcat gcccggcttg agcggggctg atggctgggc ttttttgcgt cggtttcctc
     4981 gggaggccgc tgaccgtaaa gggtcgtttt ctggctcgtc ttttttgcag tcgcttttgg
     5041 gttggtgaga caacctttaa gacgcacatc cgaccgcttc cggtttcctc cgttttagac
     5101 gccgctgcgg tcgtgacgct atcctgcagc tggtttggtt tccagcagca cactcaagcc
     5161 cggcaccgtt ttggtgtcgg gctttttttc gcccgtaagg agggcacatg aaatcaaaga
     5221 ctgaattacg cgctgccgcg acccgtcgcg cccttgaggt gatcgcttct gagatggggg
     5281 gctggtcgct cgacgggttt gctcatggat cgctgccgaa cttctccccg ctgcctagcc
     5341 agcagacgca ggagggctcg gtggtgctcg ctcgatcgcc cttcgagtgt tcgtgggctg
     5401 gcactgctgt gttcaccgct caggcgagcc ggttcgtgaa tgccgatccg gcgtctgctc
     5461 actacgagtg ggcgtgcggc gtcgaaaggg aggctgtggc caccgcgctg atggttgaag
     5521 cgttcaacgt gaccggctgc gtggagtacg ccgggttgcc tcctgtggac ggcatggtgc
     5581 tgctgaccat ggacgaggcc gacgtggagc tgatcagggc ggcgatgctg ccttggttag
     5641 atgcggcggc gtaggccgtc gctcctctgc gtcagatcag gccacgctga cgcagaggct
     5701 ggatacgcta gatggctacg gctaaagcca caggtgacgc gggctcattg agttcaggag
     5761 agcattatca ttccactttt ggttgaatct tagctgtaat ccgtatagaa ttattttact
     5821 gcaagagcgt tctacagata cgtaaattat tctgtagtca gcagtaagga gctaaaactc
     5881 ctgaccaata gcgaatttga tagacagcag aaaaccgccg atgctgctag atccgaaagg
     5941 aaccaatgaa gggaaatatt taggcgttat ggttcattct cattaaattg aggagaactg
     6001 cttaaatggg cgtccttcct atcaccctcg ctgtatagct ctacacttgg cctccgttgc
     6061 atggtatgca gttacggggg ccaaattgtt ttcagagaca tcaactatcg ccattgcgaa
     6121 aaaaccagcc ccagcgacgt taatacgacg agcagcattc agcacaatag tctgctcaga
     6181 ataatccctg ttaacgtaga cgttactctg cactgaaaat gcagtagcca aaatttccgc
     6241 acactcctgc caacttaatt tatccttcga gttacgtgca gatagcacat gcctaaacat
     6301 agcccaagta attggtattg ctggcgacgt aggcaaatcc cagccgtttc tctgacaaac
     6361 aaccttcatc tgattgaatt gttttttagt catcaaataa gtgaaacaag gagtctccca
     6421 gacttcttga gggagcccct tatcatccat ttccgcagct tgttgcgtga agatgtagaa
     6481 agcatcccgg attgcatcat ctccgatagc cactgacttt ccttataaat aatttaaggc
     6541 tgcattttat catatttaat tacctcactt aggactgcta actcagcaca atagaccact
     6601 ctcccgcagc ttatggcggt agtcccggac gcggctggtg ttccactcct gccccgtcgc
     6661 tgtccggtac ccggctgcgt tcagcgccga cgcgatggcg ggcagggtgg cgttctcccc
     6721 ggtcatgctg acgatcagct ggtcggcctc atcgctgacg gcctggaagc gtggatcggc
     6781 tccccggggg actatctgtc gtgcgctttg tcgtgcggtt tgttgtgcaa actgtcgtgc
     6841 atttttcccg cctccggcca gcgccctgac ctctgaccgc agcgcctcga tggcagtcaa
     6901 aagtttggtg tccatccctt caggcttcgt gccgagtccg gcgacgagcg cctctatgac
     6961 ggcatctgtg cgggttactt tggttttctc cgctcgctgc tcgatgcgct cgatcaggct
     7021 gacgggcagt cgaacggtca gggttttcgt tttctctcgc atgggctagc atctccgtgt
     7081 cgtgctattt gtcgtgctat ttgtcgtgct gtttgtcttg cggagtgtaa tgcgttttgt
     7141 cgtgcgcgtc gaatttgacg tagtgcaatt gtcgtgcgct gaggggcgac ggcgttgcgt
     7201 cgccggggtg cctgtggaca gtttctgtgg acaagtattt tgcggcggag ctggcccgtg
     7261 gataagtggt agaggctacg cggctgtaag gctggagctg gatcgcgttt cgcgtggttg
     7321 gatcgttgtg tgctggtaca ggggggaatg taacggtatg atgacgcggc tgaaaaagaa
     7381 aacccgtcct ggcagacggg tcattttctt taaagctgtt gttgttaaaa ctttaaggag
     7441 ctttcgcggc gggaaccgtg gaagacaccg attctaacaa caggttgggt ggcaatccag
     7501 cttgaattgt acgcgaactt tccagcgcgt gcaaagggtg ttttccccgt aaagtcccgc
     7561 cgttgtaggg ctcctcaaga ttcgatgtca attttgagga tgtaaaattg cctaatcatg
     7621 atgataattc tggcgctgct gtagccactg taacgcagat cgccgccgcc aaggccaaag
     7681 ctgccgccaa gaccgagcgc cctgcgttca tgggtggttc tctgcctcct gtcccgttca
     7741 atgagtggga tgggctcacc ggtagcccga cccgcgacgg tttccgcatt caggcggctg
     7801 aaaagcgatc ggtaaatttc ggcaatgagt atccactgcg gtatgtcccg ccgctgatca
     7861 ctgatgctgg ctctgggcgc tgggacgtcc tcaatcacgc cgacaatctc cgctggttgc
     7921 tctaccgcaa tggtgcccgg atggagctta acctcatgac cttcaagaat gagctctcat
     7981 gggctgatag cggctctctg gtcgaaccgg acgagcatgg tgcccgcgcc tggatactga
     8041 acaaaagcgc cgaggttggc ctgaaacgtc aggctgttga cgatttcatg ttctacgttg
     8101 cccgttccgc tccctaccac cctgttgccc gcctcctcgg ttcccgcgag tgggacggtt
     8161 ctcctcgcgt cgatgccgtg ctggactgcg tacctgtcgc acctggtaag gaggcgctgc
     8221 gctcggctct gctgcgtgga ctgctgattg cggcgatcac cgctgttgag gaaggggctg
     8281 tgtcgaacaa gtacgttcct tgcctctact ccgaggctaa caactggttt aagacggcgt
     8341 tctttcgcgc tctgttcgac ctgctgcctg gtgcgttcgg gcagggtgtg tctatcaacc
     8401 cttcgagtaa ggacagcgtg cgccctgcgg tcatgcgctg gtttaacgag ctgggtgaag
     8461 tgtcgggcat gatgaagaaa aacgaccccg atgaaatcaa ggcgttcgtg gagcgcaacg
     8521 tggatgagtg gcgtcctgaa aacgagaagg cggtgatcgc caaaaagcgc cagatggtga
     8581 tgggtgccac ggtgaacaag cgcgactacc tgaaggacga gacggcggcg tcccgcttcc
     8641 cgaccattac cctcgtcgcc cctatcgaca ttgctcgcgt taacgagctg ctcgggtggg
     8701 agtgggataa cgtcaaaggc tgcgctgttc gggcggatga ggatcagctg atccagttct
     8761 ggctcgaggt tcgtcacctg taccgctctg gcgtcggttt caacctggac gacgagacct
     8821 ttgctgcggc gaaagctgag acggctcaat tcgtcgatcg cggctcgtac tatgaaccgg
     8881 tgcgcgacat cctcctgttg cctggcaatg cgtccactgg ccagtacccg tgggataagc
     8941 ctggctatga cggcaggttt accgcgacgg acgtgacggc ggcgctgcgt gccccatcaa
     9001 gccattctgg tcgcgtcggt agggcgttga agcgcctagc tgatgagggg atcgttgatc
     9061 gcaggaacca ccggggtact tttgtttact ggaaattgcc agacgaacca accggggaac
     9121 tcccgctctg aaaatgaccc gcttcggcgg gtttttttat gcctctgcca atgttggggt
     9181 acccgtgtgg gggttccgtt ttgtgtgggg gtatggggtg atcatgtggg ggttcggtgt
     9241 gggggtaacg tgtgggggta actggtgatg ttggggtaag atgtgggggt aaactcgcaa
     9301 agaaaatcta cctccacata ctcaaaatcc ctttcatggc gctgcatgtg gctttcgtgt
     9361 tggggtatgt gggggtacat tcttttattt atctactatg taaaaagagg ggtaataagt
     9421 agcccccaca ccccctgtac gcccatatgt tacccctaca ccctcatacc cccacacgct
     9481 caaaaattgc gcagttggcg tgccgcagct gcacccctga aggggatgca ctttgcttgc
     9541 cttgtgaggt ggggtgatcg ggggcgcaga ggacgaagct gtcgcttcgc tgggtggtcg
     9601 tctggtcgtt ctgcgtgagg gcttgccctc gcgccctctg cggcttgtgt ggaatgcgta
     9661 gctgcagctg cgcgtttggt tcgtccgggc atcaaaacgg gacttctcgc gcacgcgagc
     9721 ggtaacaatt gcgtaacgtg cggatccgcg tattccgggg gctcccgggt tttctccggc
     9781 ggtaaaacgg cggtaaaagg cggctttccc gccgttttca gctctgctgg cggttcatgc
     9841 cctgccgcat gatgtcggcc tcggtgcccg gcttgatctc gtagatgagg cgctcgcgtt
     9901 cctgcgcctt aagcagggag gtcatgaggc cgctgatcgc tttgaccatg gtggcgtcct
     9961 tctccagcat ggcctgtagc gatgcgctgc gtagggcttg caggtcgctc gcgtggcgct
    10021 ggagtagctc gtactggcgc tgcacaaagt ggtttttgag gcgtcggact gcggcctgtg
    10081 gggtggcctc gacggctcgc tgcgcgcgtt cgcgcatctc ctcgatgctg aggacgttgc
    10141 ttggtgcgtc ggcgcgggct ttcgcccaat ctctgacggt gcggggtggg atccccatgg
    10201 tgcgtgcgac ggtggcggcg gttgcgccgt cctgctccag tagcttgagc gcctgggctt
    10261 tctgttcggg ggtgtatttc ttcatggttc ctgctccggt tgttcgttcc tctccattgg
    10321 aacgtcacgc gctgaaactg gcggcaactt ttggggggtg tgcggcgctg tcgcgtgctt
    10381 ggtggtacgt cggtgcggtt ttgtggctga ggggcttaca gcgcgtctga gggcttctgg
    10441 tggattgggg cgctttcacg ccccgggtgt aaggtttggc tacggtgtaa gtaagaatca
    10501 cctacaccgt aagcggcgct taattttcaa tgaggcggtc gatctcctcg gcgacgttga
    10561 ccagcctgac gtcggtgcgg cggctggcgc tgcaaagggt gtaatcgctg aggtacctac
    10621 cccatgcgac cacttcgtcg cgggtgttga ggagcgccca tcgtgcgccg ttgttggcca
    10681 gtttcgtcca gcgtgccagc gccttgaggc gtgggcttag gcggtaggtc atgctgccac
    10741 ctcacggcgt aggcggcgca tggctctgcg ccaggtctgc gggttgcggg tggttactgc
    10801 gcggcgtgcc agctggcgtg cgacgctgtt gtgcgatacg atcatggggt taacctcttt
    10861 cattgggtgg tttaacgcgc ccggttggct ctcacacttc ccgggcctgg gtgcggcggt
    10921 accattcgag tcaccataac tcggtaccgc tgcgctctct cttactccgg cgctttcgcg
    10981 tcgatcttct caagacaccg gtaaattgcg ctccggctta gttttagctg cttggctatg
    11041 ctggcgatcg gcactcccat ggcgcgtcgc gtcacaattt cgccgacttc cgtggctccg
    11101 atcttggtcg ggcgtccgaa cttctggcct cgctttttcg ctgcggcgat ccctgctgcc
    11161 tggcgttcga ggatgagtgt tctctccatt tctgcgaagc tggccaccac gcctattacc
    11221 gactttcctg ttgcggtgtt tagctccacc ccctcgcgta ggctgatgag gttgatcttc
    11281 cgcttctcca tctcctcgac cagctcgagg gtgtcccggg cgttcctgcc gatgcggtcg
    11341 atcgccgcaa ctaccagggt gtcgccctcc tgcaggatct ggttaaggag gatgccaagg
    11401 gtcggacgct cgcgtggtgg taccgtcccg ctcattggtt cttccccaag gacgagggcg
    11461 tcggggtagt gcgcggcgat ggcgtcccgc tgcccgtcgg cggtctgttc cctggttgag
    11521 acacgggtgt agccgtatgt tttgttcact tttcgctctc ttacgctttc gcgtgcttct
    11581 gtaaaattct gttcaccgtt cccctgctga tgcctaggtc gcgtgctatg cgtgcggcgc
    11641 ttactcctgc atcgtgcttc atgatcaccg ctccgacgtt ctccggcgtc tgtttggtcg
    11701 gtcgtcctgc gtggcgtcct gcggctgcgg ctgctgccat ccctgccttt gtgcgctcct
    11761 tgagggtggc gagttccatc tctgcgaagg cagcgaccat ggtgagcatc atcttcccgg
    11821 tcggggtgct gaagtcgaaa ccttcgcgga ggctgatcag gctgacctgc cgcttctcca
    11881 gctcctcgca gacctccatg accgatctgg cgttgcgtcc gatgcggtcg atcgcgacca
    11941 ccacgatgct gtcgcctggt gcggcaaact ccaggatggc tttgaacttg tcgcgctggg
    12001 tggcgggtac cgttccgctg atgccctcgt cggcgtggaa ggtggcttgt gggtagcgat
    12061 cgaggatcgc tttcttctgt ccggctactg tctgttctcc ggtgctgacg cgcccgtacc
    12121 cgaatactct gctcatgctg tctcctgttg tacaaaaaag gttgtttgtg gatatttgac
    12181 cacttatgag caaaaagcaa gcgcaaaaaa ctgtaaacgc ctagagccac gggggctgcg
    12241 cagtttcgct cctttttgga cgtgatcacc ccgcgctggg cgagagccct gcgccctctg
    12301 cgtgggtgtg ctcggttggt cgtgacggca tgctgtctga gcggcgtttc ttgccgcttt
    12361 gctgataact tgctgagaac ctaaccatga ccgaaattaa gaaagagagc gccgacggcg
    12421 cttggctacg ctctaccctt gccgaattgg cgggtgatgc gttccctgtg tatgacctcc
    12481 cgtccctgca ggtagacacg aactcctcca ttcagctctc ggcgctcgcc gatcgtcagg
    12541 ctgcgcgtga aatgcgacag gccgcttccg acgtggaagc gcgacggctg gacgccgctc
    12601 gagtggtgga agggctcaag accgaggccg aacggctgcg gggtttgatc gccgacggca
    12661 aggctgcgct gcgtgctggc gagccggtga gccccgacgc gggtgttgcg tcgttcctgc
    12721 tgccggacat cgaggccgag ctggttgttg ctgaggctgc tgaggccgac gtggcccggg
    12781 agcgtgacac cctgctgcag gatgccgatc gccgtgacgc ggcggctgcg ctggcgctgt
    12841 tcaactgggc gcactccgtt cgcgtgcagc g
//
