LOCUS       NZ_CP055417             5585 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00054 plasmid pRHBSTW-00054_6,
            complete sequence.
ACCESSION   NZ_CP055417
VERSION     NZ_CP055417.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148463
            Assembly: GCF_013590775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00054
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 94.3779644747279x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:40:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,369
            CDSs (total)                      :: 6,252
            Genes (coding)                    :: 6,043
            CDSs (with protein)               :: 6,043
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 61 of 209
            Pseudo Genes (incomplete)         :: 163 of 209
            Pseudo Genes (internal stop)      :: 29 of 209
            Pseudo Genes (multiple problems)  :: 36 of 209
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055417.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00054"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00054_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(28..1548)
                     /locus_tag="HV046_RS31620"
                     /old_locus_tag="HV046_31695"
     CDS             complement(28..1548)
                     /locus_tag="HV046_RS31620"
                     /old_locus_tag="HV046_31695"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249302.1"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]; GO:0020037 - heme binding [Evidence IEA]; GO:0046872
                     - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="WP_000076364.1"
                     /translation="MSQDPKKCPVTHLTTEAGAPVVDNQNSMTAGARGPLLAQDLWLNE
                     KLANFVREVIPERRMHAKGSGAFGTFTVTNDITQYTRAKIFSEVGKKTEMFARFSTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDLVGNNTPVFFLRDARKFPDLNKAVKRDPKTNKR
                     SATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTFSFINAQNERFWVKFHM
                     RTQQGIQNLTDAEAADLIAKDRESSQTDLFDAIERGEYPKWKMYVQIMPELEAETVPYH
                     PFDLTKVWPKGDYPLIEVGEFELNRNPENYFQDVEQAAFAPSNLVPGISYSPDRMLQAR
                     LVNYADAARYRVGVNHSQVPVNAARCPVNSNRRDGQGRMDGNYGSLPHYEPNSFNQWQE
                     QPQFKEPALKITGDADFWDFREDDNDYFSQPRALFNLMNDEQKQALFNNTAAAMGDALD
                     FIKYRHIRNCYACDPAYGQGVAKALGMTVADAQAARETDPAKGLPSFL"
                     /name="catalase"
     gene            1804..2013
                     /locus_tag="HV046_RS31625"
                     /old_locus_tag="HV046_31700"
     CDS             1804..2013
                     /locus_tag="HV046_RS31625"
                     /old_locus_tag="HV046_31700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181245240.1"
                     /translation="MAMQTGEWHRLSAGIRAESPVLSGLLQRRAVIRPLALRSFQLQRF
                     LVLRRPLLLPVRLNTVQPCDNRRS"
                     /name="hypothetical protein"
     gene            complement(1986..2201)
                     /locus_tag="HV046_RS31630"
                     /old_locus_tag="HV046_31705"
     CDS             complement(1986..2201)
                     /locus_tag="HV046_RS31630"
                     /old_locus_tag="HV046_31705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_071182205.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2205..2690)
                     /locus_tag="HV046_RS31635"
                     /old_locus_tag="HV046_31710"
     CDS             complement(2205..2690)
                     /locus_tag="HV046_RS31635"
                     /old_locus_tag="HV046_31710"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_071182206.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAAILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2725..3429)
                     /locus_tag="HV046_RS32215"
                     /old_locus_tag="HV046_31715"
                     /pseudo=""
     CDS             complement(<2725..3429)
                     /locus_tag="HV046_RS32215"
                     /old_locus_tag="HV046_31715"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3368..3691)
                     /locus_tag="HV046_RS31645"
                     /old_locus_tag="HV046_31720"
     CDS             complement(3368..3691)
                     /locus_tag="HV046_RS31645"
                     /old_locus_tag="HV046_31720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181245241.1"
                     /translation="MLTMWVTEDEHRRLLERCDSKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAE
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4341..4454
                     /locus_tag="HV046_RS32220"
     CDS             4341..4454
                     /locus_tag="HV046_RS32220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072202106.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            4762..5064
                     /locus_tag="HV046_RS31650"
                     /old_locus_tag="HV046_31725"
     CDS             4762..5064
                     /locus_tag="HV046_RS31650"
                     /old_locus_tag="HV046_31725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="WP_031942343.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     Typing          complement(3807..3980)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          3904..4450
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.273"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1883..3378)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.786"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attaactatc taaatcaaat ttccacatta aagaaaactt ggtaaacctt tagcaggatc
       61 tgtttcacgt gccgcttgag catctgctac agtcatacct aaggccttag caacgccttg
      121 cccataagct ggatcgcaag cataacagtt gcggatatga cggtatttga tgaaatctag
      181 cgcatccccc atcgctgcgg ccgtgttatt aaacaatgct tgtttctgct catcattcat
      241 caaattgaac agggcacggg gctggctgaa gtagtcattg tcatcttcgc ggaagtccca
      301 gaaatcagca tcaccggtaa tctttaatgc tggttcttta aactgaggtt gttcctgcca
      361 ctggttaaaa ctgttcggtt cataatgtgg caatgaacca tagttgccat ccatgcggcc
      421 ctgaccatca cgacgattag agtttacagg gcagcgtgca gcattgaccg gaacctgtga
      481 atgatttaca ccaacacgat aacgtgctgc atcagcatag ttcaccagac gtgcctgtaa
      541 catacgatcc ggtgagtagc taattcccgg taccagattg cttggtgcaa aggcagcctg
      601 ttctacatcc tggaaataat tttccggatt gcggttcagt tcgaattcac cgacttcaat
      661 cagtggataa tcacctttcg gccagacttt ggtcaggtca aatggatgat aaggaactgt
      721 ttcagcttcc agttccggca tgatctgaac gtacattttc cattttggat actcaccacg
      781 ctcaatggca tcaaataagt cagtctgact actttcacga tctttagcaa tcaaatctgc
      841 tgcttcagca tcggtcaggt tctggatgcc ttgctgggta cgcatatgga atttcaccca
      901 gaagcgttca ttttgggcat tgataaaact gaaggtatgg ctgccaaaac catgcatatg
      961 acggtagcca gcaggaatac cgcgatccga catcacaatc gtcacctgat gcaaggcttc
     1021 aggcaataat gtccagaaat cccagttatt ggtggcactg cgcttgttgg ttttcgggtc
     1081 acgtttgact gctttattca ggtcagggaa cttacgggca tcccgcagga aaaacaccgg
     1141 ggtattattg ccgaccagat cccagttgcc ttcttcagta tagaatttca gggcaaaacc
     1201 tcgaatatca cgttctgcat cggccgcgcc acgttcaccc gccaccgttg agaaacgcgc
     1261 aaacatttcg gtttttttgc cgacttcaga gaaaatttta gcgcgagtat attgggtaat
     1321 atcattggtc acggtaaagg taccaaaagc acctgaacct ttagcgtgca tacgacgctc
     1381 tggaatcact tcgcggacga agtttgctaa tttttcattc aaccataaat cttgggcaag
     1441 taaaggtcca cgcgcacctg ccgtcatact gttctgatta tcgaccacag gggcaccagc
     1501 ttcagtagtc aggtgggtaa caggacattt tttagggtct tgactcatac tattaactcc
     1561 cgtttctttt aatttaaatt tcctgcagaa atttaagatt ttcaatccta tcttaccctt
     1621 tacttgctta ttaaaatgtt aaactaagga tatttttgaa aatttctttt tatttaacat
     1681 aagagttctt gcccgcaccg cttcgggcgg gttcagtgcg aaagcggcgt cagcccggca
     1741 aaacaggcag aaaactcccc gctaaaccgc gcattctgcg ccagatagat ttcacctgtt
     1801 tgcatggcta tgcagacagg tgaatggcac cggctttcgg cggggatccg cgccgaaagc
     1861 cccgttttat ccggtttgtt acagcgaagg gccgtcatac gtccgctggc gctgcgatcg
     1921 ttccagctcc agcgctttct ggtgctgcgc cgcccgctgc tgctcccggt gcgcctcaat
     1981 accgttcagc cttgcgacaa ccggcgcagc tgatcgctca aactctgcac ctgcctgctc
     2041 aagcccgtga cgcgctcgct cagcgccgcg ttctcccgtt gcataagacc agacatcgtc
     2101 cgccattccg cgaaggcgct ctcccactcg tccagccttt tcgagtagtc ctgctgtagc
     2161 tgctctaatg cgctcagcaa ctgtttttcc agctccgtca tgtgctattt ccccgccagt
     2221 atcatccagc tcgaatcctc tccgaaccgt cccgcttccg ggttcacctt cacgcagcgg
     2281 cgtctctgct cgccgcaggt cgagagctgc acgccgctgt tcctctctga cagcatggcc
     2341 tgtgtgctct tctgctcccg gatggtcgtg taattgtcga gtatctgctg actctgccac
     2401 cacagaatgg ccgcactcga ggcgatcaac agcacggaca ccaggacgat ggtcagccac
     2461 gtctggctga ccatgcgcag catccctttc gtgcgctggc tcatggctga ggacagcttc
     2521 cggtcgtggt cgaggatggc ggcgctgatt ctcttctcgc tttcgctcag ttccgctctg
     2581 acagacttct cgtgctcgct gaatgcggct ttcagcatct cgccggtagt ctgctgctgc
     2641 gctttcgatt tctgctctaa gtccttcgcc agcgttaaaa gactgttcat agatggctcc
     2701 cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatgctgc ttttcgtggt
     2761 gcgcaccacc tcgaaacctg cgttttccag cgcctctgtg acgtcctgcc gcgttttcag
     2821 ctccccggat gaggcaaggg caagcaggcc gcgcgtaatc gcctgggcgg cctcctgctt
     2881 cgcttccggc agcgcggaag gcatgaccag cgcccgccgg ttttccggcg cgttcgggtc
     2941 gtgcagcccc agcctgccat taactatggt ctgccaggca tcaatgcgcg gacggtcggc
     3001 gcggtcgtaa tacggctgga gacgtctgcc ggtcagcagc tccgtgttcg ggatcaggaa
     3061 gttcagctcc agtcgccctt tatcccggtg ctccacccac agcacgctgt actggtcttt
     3121 atcgagtccg ggcatcagaa cccgctcaaa gcccgccatc agcctttcgc gctgcccggg
     3181 cggtaaatcc ttttcggcaa aggacaggac gccggaggtg tactttttgg cgtacggcga
     3241 ggcgtcgata agctcccgga cttcctccgg cttaccctgc agaacgctgg cgccgtcacg
     3301 ctgccggtct ttccccagca ggtaatccac cggaccggcg ccaccgccgc gccctctggg
     3361 atgaaattta acgatcatcc tctgccccct tttcaagcac agcgtgccgc agccgttcga
     3421 gtccggcatc gatggccatc agcgcggcga caacctgcac gcggtcgtgc ccgctgccgc
     3481 caccggcgtt aacctgtcgg gcaatctggt taaggttgtt acccatgccc gcaagctggc
     3541 gaagcagcgc cggcgagatc gagggaagtt tgccggcgcg ggcaggcttc tcgtccaggc
     3601 acgtctgccg catccaggct gcgagctgct tactgtcgca gcgctccagc agacgccggt
     3661 gctcatcctc ggtaacccac atcgtgagca ttttgctgcg tttgtctgcc aatccggcct
     3721 cctgataacg ccgggacggg cgggaaatct tttcccggtc ggcgcggcgt tggacaagcc
     3781 gcaggcgcgt cagtggcttt tagcaggttt cggggcgcag ccctgaacca gtcacgtagc
     3841 gctagcggag tgtatactgg cttactatgt tggcacggat gcggaaatca gtgaagtgct
     3901 tcatgtggaa ggagaaaaaa ggcggctccg gcgcgtcagc agaatatgcg atacaggata
     3961 tattccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg
     4021 cgggcttacg aatggggcgg aaatttcctg gaagatgcca ggaagaaact taacagggaa
     4081 acgataaggc cgcggcgaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     4141 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg acttaaagat accaggcgtt
     4201 tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     4261 tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcggttcc gggaaggcag
     4321 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc tgcgcctttt
     4381 ccggtaacta tcaacttgag tccaacccgg aaagacacga caaagcgcca ctggcagcag
     4441 ccactggtaa ctgatttaga ggagttagtc ttgaagttat gcgcctgtta aggctaaact
     4501 gaaagaacag atttggttcc tgcgctcctc ataagccagt taccacggtt caaagagttg
     4561 ctgctctgag aaccttcgaa aaaccgcctt gctgggcggt tttttcgttt acagagcaag
     4621 agattacgac gctacagaaa ggatctcaag aagatccttc accataatgg aaatctatcc
     4681 cacccgagca tataggatgc atatcgttat aaaacatcgg atattatgca tcccaactaa
     4741 aaataagctc gaggataaaa aatgagttca atggtttact gccgaggatg cggcaaggaa
     4801 atacacgaaa cagcaaagtc atgcccacac tgcggagcca caaacgcctc ttcaggttca
     4861 ggtgaaaaaa gtagaatcgc agccgcgcta ttagcattct tccttggtgg atttggcgca
     4921 cataaatttt acttaggtaa aataggacag ggattcctgt atctaatttt ttgctggaca
     4981 ttcatacccg cgatcatcgc gtttattgag tttattattt atctatgcga ttccgatgaa
     5041 aaattcgcaa gaaaatatgg ctaactcata gcaaaaaagc cctgcggggc tttttttgta
     5101 ccagtctgtg aggcagattt accataacat ccgttatccg aaatcttctt tcataagtta
     5161 tataaaatta tatacttaac tatttcacat catccaaaaa ttcaccaaaa cgccgacatc
     5221 gacacatctc ggcgtttttt taagcgcttt tgtcatattc cgccaataac cggttaaaaa
     5281 atgatcaaaa ttatatcttt tactgacttc ttaagccttc cattttcgaa tttatcccca
     5341 ttttctgcgg acaaatatcg ggataatttt ttgggtcgtt cactgtacgt tctggcttac
     5401 agccatgcag gaaattttcc aataggcaga tgatcatcga tttcctatta tggactcatc
     5461 aagaacagga tgttcgaaga cctaaaacaa taatcataag tcaaacacca ggatcgtgaa
     5521 gctagacagg agtcatgctt aagcaccaaa tacgaaaaac cccgacatcc tgtcgggttt
     5581 ttttt
//
