LOCUS       NZ_CP055416             9581 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00054 plasmid pRHBSTW-00054_5,
            complete sequence.
ACCESSION   NZ_CP055416
VERSION     NZ_CP055416.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148463
            Assembly: GCF_013590775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00054
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 94.3779644747279x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:40:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,369
            CDSs (total)                      :: 6,252
            Genes (coding)                    :: 6,043
            CDSs (with protein)               :: 6,043
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 61 of 209
            Pseudo Genes (incomplete)         :: 163 of 209
            Pseudo Genes (internal stop)      :: 29 of 209
            Pseudo Genes (multiple problems)  :: 36 of 209
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055416.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00054"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00054_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            243..434
                     /locus_tag="HV046_RS31565"
                     /old_locus_tag="HV046_31640"
     CDS             243..434
                     /locus_tag="HV046_RS31565"
                     /old_locus_tag="HV046_31640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            434..856
                     /locus_tag="HV046_RS31570"
                     /old_locus_tag="HV046_31645"
     CDS             434..856
                     /locus_tag="HV046_RS31570"
                     /old_locus_tag="HV046_31645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_015062851.1"
                     /translation="MARVSISEAAQLVKVSRPTIYKMINSGKLSYTSVVKHGKSIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDSESTGINSVVLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPYSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1960..3067)
                     /locus_tag="HV046_RS31580"
                     /old_locus_tag="HV046_31655"
                     /pseudo=""
     CDS             complement(1960..3067)
                     /locus_tag="HV046_RS31580"
                     /old_locus_tag="HV046_31655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(<3141..3526)
                     /locus_tag="HV046_RS31585"
                     /old_locus_tag="HV046_31660"
                     /pseudo=""
     CDS             complement(<3141..3526)
                     /locus_tag="HV046_RS31585"
                     /old_locus_tag="HV046_31660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(3748..4683)
                     /locus_tag="HV046_RS31590"
                     /old_locus_tag="HV046_31665"
     CDS             complement(3748..4683)
                     /locus_tag="HV046_RS31590"
                     /old_locus_tag="HV046_31665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198895.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="WP_228724875.1"
                     /translation="MMLLSIIIPCYNCESNIEKLLLSIKEQLNETVQLVLINDGSWDAT
                     HDIITKFTSDNPSIKIKYINSINQGAAKARSTGLNYAEGDYVFFCDSDDMISANFISVF
                     TELSNDKPDMIYFCSTVLDSQGNRFKKVHFPLAYIESNSNEVFNYLLKKGQWTAAVWTY
                     IFRKDLVLKSGAIFTDRKVHEDHLFTLRLLAGSEKISICNSELYFQNVTSGSLTKSPKK
                     IDYLTERYKSYKEAREDIIYKFSQESIVLYDRWSMSSLLQILKENKKMVFQGIMYTAIY
                     RVLWKEKLVILDFVKSKVKKKITRSKSLSQ"
                     /name="glycosyltransferase"
     gene            complement(4680..6146)
                     /locus_tag="HV046_RS31595"
                     /old_locus_tag="HV046_31670"
     CDS             complement(4680..6146)
                     /locus_tag="HV046_RS31595"
                     /old_locus_tag="HV046_31670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="WP_167876629.1"
                     /translation="MNLKSKTIHAVKWNLLSTVCVTGLGMVSLWILSSLFTTTDYGIIS
                     AALILSTVFSIITDFGISNSVIRSEKLNKYELSSLYIINVFLGMIFCLGLFIFSAQLAS
                     LFNGGTELAKQIKIMSFSLIISSFGGQPRALLARGLRFDVISRITILSAIFNFVFAVFL
                     SYVYRSPWCVAVSLLVSSSISSVLYIYSSKGLRDIKLQFFTLKEIKKHVSYGSQLVMDS
                     IINQISVNTYPVLMARLISLTAIGGYNISYNISVALFEKLNPVLSHALFPAFSKIQNDA
                     ASLKKTFLKVTMFSSLVNFPMLLGMMLISDYVVTVFFEQKWQFITPIVQILCLVGAARS
                     LDVPVISVLLAKGQMYRNVYLGVFKLALGIPLTWFLGKQYGLTGIVFSFLCIQIVNLVM
                     GYFFQVRPCLNIKVKSYIYAVFVPLIHTLPMLVTGYLLILFLTITSPILSMVVIIFSCM
                     SVYIASILTSPVKEIKEFNAIVLNNILRNK"
                     /name="MOP flippase family protein"
     gene            complement(6427..7254)
                     /locus_tag="HV046_RS31600"
                     /old_locus_tag="HV046_31675"
     CDS             complement(6427..7254)
                     /locus_tag="HV046_RS31600"
                     /old_locus_tag="HV046_31675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_074177990.1"
                     /translation="MISVNLTTMHSRLDLCAATVWSIINQSVQPDKINLWISNEPYLSD
                     HGIYEIPAWVEELNKINNIVNVHYVKNTGPYRKIINAIINSEIDDILVYADDDVIYGRQ
                     WLEFLLDQFYENNCNVAIAARIRIMERNIFNKYKSYSSYSISMKKDKFEKDFIITGVGG
                     CVLSKRMIKPSIIGDDAYLTISPKTDDIWISKMLEASNTKVESSPDALRCVHEILHGYN
                     TLSSINTLTSKNSSLIIKAIRMVKNKFYSYFGLQKTNNDKSIKMVNLHFNGKL"
                     /name="glycosyltransferase family A protein"
     gene            7436..8134
                     /locus_tag="HV046_RS31605"
                     /old_locus_tag="HV046_31680"
                     /pseudo=""
     CDS             7436..8134
                     /locus_tag="HV046_RS31605"
                     /old_locus_tag="HV046_31680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(8509..8622)
                     /locus_tag="HV046_RS31610"
                     /old_locus_tag="HV046_31685"
     CDS             complement(8509..8622)
                     /locus_tag="HV046_RS31610"
                     /old_locus_tag="HV046_31685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            9194..9343
                     /locus_tag="HV046_RS31615"
                     /old_locus_tag="HV046_31690"
     CDS             9194..9343
                     /locus_tag="HV046_RS31615"
                     /old_locus_tag="HV046_31690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015062850.1"
                     /translation="MPSEHDEQWDAADTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          8987..9201
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.364"
                     /coverage="100.0"
                     /evalue="3.53e-41"
     Typing          complement(8514..9062)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.948"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 ggtgacgaaa aagcgtcctt agcgacctcg ttcatgctcg catgaacgga ttatttttcc
       61 ggactacagg taaactacag aaagactaca aaagctgatg acttaatcct gatttttagt
      121 gcgatgacta cagataaact acaaaataac tacaggatta cctcagattc gttctctgaa
      181 ccattcgtac atccgacccc cttaccggtt ttacactgag cactgatgct ctgaggtccc
      241 ttatgacgaa acaacagaaa acagcgctga acatggctaa attcatccag aatcagtctc
      301 tgctcctgct ggagaagctt aacgagcttg atctcgatgt cgaggccgac ctttgtgaga
      361 aactccacga tgatgcggag catcttttcc gtactctgtc ctctagactg gatgcattac
      421 aggatggtaa ctgatggcca gagtgtctat tagtgaagcg gcacagcttg ttaaggttag
      481 tcgccccacg atttacaaga tgattaacag tgggaagtta agttacacct ctgttgtaaa
      541 gcacgggaag tctattaaag ttattgatac ttcagagctt attagggtgt ttggttctct
      601 tgatggtgta atcgatactg taaaatatga tgtaaaaagt gactcagaat ctacaggtat
      661 taacagcgtt gttttacatg atttacaaca tagaatcgct ttacttgaag ctgaaaacga
      721 tggtcttaaa ggggctgtta aggctcgaga tgagcatatt gattcccttc gtcaggctat
      781 gcagcttctg gaacacaagc acgaaccttc ttcaccaccc tattccccgt ggtggaaatt
      841 ctggaaaaaa tcctgatccc gccatgcttc agttttcctc tccggtcatc ccggggcgtt
      901 cctgaacctt cccagatacc gccagatctc tgaacgctcc tcaccactaa aaatacccat
      961 acccacaccc cacgcaaaat taaaattttg aagataactc attaatatca gttagttagt
     1021 tgttattaaa ttacgcagta ttaccttctg taaggtcaat ccaagtcgtc ggcgattcgt
     1081 gtacgacttg ccgacgactc gtcatagagt catcgacgat tcactccaca ctatcagtaa
     1141 cttatgacga cttgactcta gagtcgaatc gtcattttgg tgtttttttg accttgacga
     1201 ctctagagtc aagtcacgag tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac
     1261 ttgccataac gtgattgccg gccgtcgacg aacccgattc ctgaacgtgc tgaaaatcgg
     1321 ttttccggct gcgccggcaa cgcttcatgg ccacgcaata aattgcgtcc ccgttcaccg
     1381 ttttcggatg ggaagaacgc cggaaacagg acttaccccg gctgaatggc gtgaatttcc
     1441 gcgctatgca cttgcgtgca tactcatgca tgccgtaaaa acagagccag cgcgtttctg
     1501 gcggattttc ggggggggtg ttgcctgttt taccggtttc ctgccagaaa cgccctgagg
     1561 ccgttttcgc ggtgcgcgta agaagggatt atggtaaata gggcgctatc gcctaaatac
     1621 acggctgatt gttttttttc tctgaagtat gagattcctg agtagacagc tacaacaagt
     1681 agcgcaatgg cgatagcaag tgaaatccag tccataaatg cctgattaat ttaataaaaa
     1741 cagcctgtta tgatttcata aagataatat catctttttt ttagcatgat actgtcgtta
     1801 cttattgtgg tgagatgaaa cataaccgct gtttttgtat gtgtttgtta aggctgttaa
     1861 gattattcct aaccactgat tttgtacata tcatcaaaaa aataaaaaaa ctaatgccct
     1921 gtggtgggta tgacctgccc ccaggattag atacaacctt cagttagtaa tgtcggttcg
     1981 tttttcttca tatttcccgt ttcgccagcc cgccgcaaat tcagccggag tctggtagtt
     2041 cagcgatgaa tgtggacgac actcgctcta atcctgccgc cagtcattaa tcgttttcct
     2101 cgcgtgaacg atatcgcgga accagtgctc attgaggcac tcatccctga atcgtccgtt
     2161 aaaactttca ataaatccgt tctgtgttgg cctgcccggc tggataagcc gcagctccac
     2221 cccatgctca taagcccact ggtcgagtgc tctgcaggta aactccgccc cctggtcagt
     2281 tcttatcgtc gccggatagc cgcgaaacag tgcaatgctg tccagcattc gcgtgacctg
     2341 aacgcctgaa atcccaaagg cggcggtaat tgtcagacac tccttcgtga agtcgtccac
     2401 gcaggtcagg cacttgatcc tgcgaccggt ggccagcgcg tccatgacaa aatccatcgg
     2461 ccaggtcagg ttgggcgcct ccgggcggag cagcggcaga cgttctgttg ccagaccttt
     2521 acgacgtcgt ctgcgtttta cacccagccc gttaagatgg taaagacggt acacgcgctt
     2581 gtgattaaca tgaaggcctt cacggcgcag taactgccag atgcgtcggt agccaaaacg
     2641 cctgcgctcc agtgccagct cagtgatgtg ccctgataaa tgcgcatcag ccgcgggacg
     2701 ctgagcctca tagcggcagg tcgacaggga caaacctgta agcctgcagg cacgacgttg
     2761 cgacagaccg gtcgcatcac acataaactc aacggcttcc gtttctggtc tgtcgtcagt
     2821 actttcgccc cagagccacc tgaagtgcct ccttatccag catggcttcg gcaagcagtt
     2881 tcttgagtct ggcgttctct tcctcaagcg acttaaggcg cttaacctcg ggcacctcca
     2941 taccgccata catcttacgc caggtgtaaa agatggcgtc ggaaatggcg tgcttacggg
     3001 cagaaacccc ggcttcggcc tcgcggagaa tactgatgat ctgttcgtcg gaaaaacgct
     3061 tcttcatgag gatgtcctca tgtggcttat gaagacatta ctaacatcac ggtgtattaa
     3121 ccaacgggga gcaggtcact gtcatacgcg taaaacagcc agcgctgacg tgatttagca
     3181 ccgacgtagc cccactgttc gtccatttcc gtgcagacga tgacgtcact gccaggatgt
     3241 atgcgcgagg ttaccgactg cggactgagt tttttaagtg acgtaaaatc gtgttgaggc
     3301 cagcgcccat aatgcgggcg ctggcccggc atccaacgcc attcatggcc atatcaatga
     3361 ttttctggtg cgtaccgggc tgagaggcgg tgtaagtgaa ctgtagctgc catgttttac
     3421 ggcagtgaga gcagagatag cgctgatgtc cggcagtgct tttgccgtta cgcaccaccc
     3481 cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccacgcaa gcaccttaaa
     3541 aacaccatca tacactaaat cagtaagttg gcagcatcac ccagaaagac cataaaatta
     3601 gcctataacg gcaaatagta atgctgccaa cctgttggtg accatttgaa tctgtagcca
     3661 ttcaatcctt gtaacatcag gtgatcctgg cgtcagtgaa aatgcgaaat caacaaattg
     3721 gcatcattac ccattatacg ataatcccta ttgggatagg ctcttagatc tcgtgatttt
     3781 ttttttaact ttgcttttaa caaaatctaa aatcactaat ttttccttcc acaatactcg
     3841 atatatagct gtatacatta tcccctgaaa caccattttt ttattttctt ttaatatttg
     3901 cagtagcgaa gacatagacc atcgatcata aagaacaatg ctttcctgac tgaatttgta
     3961 aatgatatct tccctggcct ctttataaga cttataacgc tcagttaaat aatcaatttt
     4021 tttaggggat ttagtaagtg aacctgaagt cacattctga aagtaaagct cagaattaca
     4081 tattgatatt ttttcactac cagcgagaag tctcagcgtg aataagtgat cttcatgtac
     4141 ctttctgtct gtaaatatag cgccagattt caatacgaga tctttccgga aaatataagt
     4201 ccatacagca gctgtccact gccctttctt caaaagataa ttaaaaactt cgttactatt
     4261 gctttcgatg tatgctaatg gaaaatgtac ctttttaaac ctgtttcctt gcgaatctaa
     4321 taccgtagag caaaaatata tcatatccgg tttatcatta cttaactctg taaaaaccga
     4381 tatgaaatta gcagaaatca tatcatctga atcacagaag aatacataat ctccctctgc
     4441 gtaatttaag cccgttgatc tggcttttgc cgccccctga ttaatactat taatgtattt
     4501 tatttttata gaggggttgt cacttgtaaa tttagttatt atgtcatgcg ttgcgtccca
     4561 tgagccatca ttaatcaaca ctagttgtac ggtttcatta agctgctctt ttattgagag
     4621 aagcaatttt tcgatatttg actcacagtt ataacatggt atgattattg ataataacat
     4681 catttattcc tcagtatatt gtttagaacg attgcgttaa attcctttat ctcttttact
     4741 ggagaggtga gtatagaagc aatgtataca gacatacatg aaaaaataat cacaaccatt
     4801 gacaaaatag gagaggtaat agttaaaaaa agaatcaaca ggtaacccgt tacaagcata
     4861 ggtaaagtgt gtataagcgg tacgaagacg gcatagatgt aactctttac tttgatattt
     4921 aaacagggtc taacctggaa aaaatatccc ataacaagat ttactatttg aatacataag
     4981 aatgaaaaca ctatccctgt aagaccatat tgtttaccca gaaaccatgt taaaggaata
     5041 ccaagcgcta acttaaatac tcccaggtaa acatttctgt acatttgccc tttagcaagc
     5101 aagacagaga taacaggcac atcaagggat cgcgccgccc caaccaaaca taaaatctgt
     5161 acaatagggg tgatgaactg ccatttctgc tcaaaaaaaa cagtcactac atagtctgaa
     5221 attaacatca ttcccaataa catcgggaaa ttcaccaaag acgaaaacat agttactttt
     5281 agaaaagttt ttttcagaga tgctgcatca ttttgtatct tggaaaaagc tggaaataac
     5341 gcatgtgata aaacaggatt caatttttca aaaagcgcca cgctaatgtt gtaggatata
     5401 ttatatccac cgatagcagt caaacttatt aagcgtgcca ttagtacagg atatgtattc
     5461 acgcttatct gattaatgat agagtccatg accaactgag aaccgtagct gacatgtttt
     5521 tttatttcct tcaaagtaaa gaactgtaac ttaatatctc tcaagccttt gcttgaatat
     5581 atatacaata cgctactaat agatgaggaa accaacagtg aaacggcaac acaccagggg
     5641 gaacgataga cgtagcttaa gaagactgcg aacacaaaat taaaaatagc tgacaggatt
     5701 gttatcctgg atattacatc aaatctaagt cctctggcta acaaagctct tggctgtcca
     5761 ccgaaagacg atattataag agaaaatgac atgattttta tctgttttgc cagttctgtt
     5821 ccaccattga ataaggatgc taactgagca ctaaaaataa acaagccgag acaaaatatc
     5881 atacctaaaa agacatttat tatatacagt gaagacagtt catatttatt taacttttca
     5941 gatcttataa ctgagtttga tataccaaaa tcagttatga ttgagaaaac agtacttaat
     6001 attaatgccg cactaatgat accataatct gtcgttgtaa ataagctgga caagatccac
     6061 agtgagacca taccaagccc ggttacgcac actgtagaca acaaattcca cttcacagca
     6121 tgtattgttt tcgattttaa attcatattt attgtcagtg atataaaata attacattgg
     6181 tgtactaaaa ggaatttttt atacctttca gcgttagagc ctatcccaaa gtaatgctgc
     6241 caacctgttg gtgaccattt gaatctgtag ccattcaacc cttgtaatat caggtgatcc
     6301 tggcgtcagt gaaaatgcga aatcaataaa ttggcatcat tacccaaaaa gcaataacaa
     6361 tttgctaacc actacagttt actgataaca ttttgttttc agacaatccc tgatgagaaa
     6421 gatcatttat aatttaccat taaaatgcaa attaaccatc ttgatggatt tgtcattgtt
     6481 tgttttttgc aacccaaaat atgaatagaa tttgttttta accatcctga ttgcctttat
     6541 aattaaactg ctatttttag atgttaatgt atttattgag ctaagtgtgt tatagccatg
     6601 caatatttca tgaacacatc gcagtgcatc tggggatgac tcgaccttcg tattagaagc
     6661 ctctaacatt ttactaatcc agatatcatc tgtcttaggt gaaatagtta aatatgcatc
     6721 atctccgata atgctaggct taatcattct cttacttaaa acacaacccc caacgccagt
     6781 tattataaaa tccttttcaa atttatcctt tttcatacta atagaataag agctataaga
     6841 tttgtacttg ttaaaaatat tcctctccat tattctaatc ctagcagcaa ttgccacatt
     6901 acagttgttc tcataaaact ggtctaatag gaactcaagc cactgccgac catatattac
     6961 atcatcatca gcatatacaa gaatgtcatc aatctctgag ttaataattg cgttaattat
     7021 tttacggtat gggccggtat tttttacata atgtacattc actatgttat ttattttatt
     7081 tagctcttca acccaagcag gtatctcata tatcccatga tctgacaaat atggctcatt
     7141 tgatatccaa aggtttattt tatctggctg aacactctgg tttataattg accacactgt
     7201 ggcggcacat aaatccagtc gagaatgcat tgtagttaaa ttaactgata tcatgctacc
     7261 ctttaaagaa aaaattaatt taatgttata ttatatgtaa aaaggttaca tattcaatat
     7321 gtaggacgtt ttttcgaaaa aatcagtttt tttcctgatg aaccttcctc cgcgttgccc
     7381 ggtaatgctg ccaacttact gatttagtgt atgatggtga ttttaaggtg cttgcgtggc
     7441 ttccatttcc atcagatgcc cttcctgctc cgctactgaa ggcgtggtgc gtaacggcaa
     7501 aagcactgcc ggacatcagc gctatctctg ctctcactgc cataaaacat ggcagctaca
     7561 gttcacttac accgcctctc agcccggtac gcaccagaaa atcattgata tggccatgaa
     7621 tggcgttgga tgccgggcaa ccgcccgcat tatgggcgct ggcctcaaca cgattttacg
     7681 tcacttaaaa aactcagtcc gcagtcggta acctcgcgca tacatccggg cagtgacgtc
     7741 atcgtctgca cggaaatgga cgaacagtgg ggctacgtcg gtgctaaatc acgtcagcgc
     7801 tggctgtttt acgcgtatga caggatacgg aggacggttg tggcgcatgt cttcggtgaa
     7861 cgcactctgg ccacactgga gcgtcttctg agcctgctgt caccctttga cgtggtgata
     7921 tggatgacgg atggctggcc gctgtatgaa tcacgcctga agggaaagct gccacgttat
     7981 cagcaagcgt tatactcagc gcattgagcg acataacctg aatctgagac aacatctggc
     8041 aaggctggga cggaagtcac tgtcgttctc aaaatcggtg gagctgcatg acaaggtcat
     8101 cgggcattat ctgagcataa aacactatca gtaagttgga gtcattaccg ggatatcgtt
     8161 caccacggga atacctgcgg cggcgaacca gtaatgggtt aagtgataaa aagtgtatgg
     8221 aaatataggg gccaatccag ctcttcagtc aatgatggtt ttgatgagcc tgaacattga
     8281 tatcgtcgta gatcttgaaa ggatcttctt gagatccgtt tttgaacgtc gtaatctctt
     8341 gctctgtaaa cgaaaaaacc gcctttgcgg ggcggttttt ccgaaggttc gtagagtttg
     8401 cggctctttg aaccaaggta actggctagt tagaggagcg cagtcaccaa aactgttcgt
     8461 ttagtttagc cttaaccggc gcataacttc aagactatct cctctaaatc agttaccagt
     8521 ggctgctgcc agtggcgctt ttgcatgtct ttccgggttg gactcaagac gatagttacc
     8581 ggataaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     8641 aactgcctac ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg
     8701 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg
     8761 gaaacgtctg gtatctttaa gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     8821 ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggcttcgccg cgaccttatc
     8881 gcttccctgt taagcttctc cctggcatct actaccagga agtttccgtc ccgcccgtaa
     8941 gccggtaccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9001 gaatatcttc tgtatcgcat attctgctta cgctctgctg cagcattttt ctcctgccac
     9061 atgaggcgct ttcctgatta tcgcactcat gccaacataa taagccatta tacactctgc
     9121 tagcgctacg tgactggttc agggttgcgc cccgaaaccc gctaacaacc tctcagctac
     9181 tggatatcat cccatgccat ctgaacatga tgaacaatgg gatgctgctg atacgcagct
     9241 tcccggcgaa tatgaccttt ttgatttaat ggcgcaggtt actgaagaga acctgcatgc
     9301 ccctgtggat tccgggccac ctgtcggccg ggaagcgtta tagctttttc gcttgcccgc
     9361 gtacgtggta atacgccggt attacatttt atttctttat tttcatgtga ttacaggtga
     9421 ttgtaatacg ttgcgtatta cataaataag ctggaatttc agtttttacc ttttaattca
     9481 tgcagttgcg atgaaaatcg cggctgcgtt aggtgtatga ccatttaagg ggcaaaaaat
     9541 tatcattctg gccgggtgta ctgcgcaggt taaggagcat c
//
