LOCUS       NZ_CP055329             1443 bp    DNA     circular CON 09-APR-2024
DEFINITION  Klebsiella michiganensis strain RHBSTW-00409 plasmid
            pRHBSTW-00409_5, complete sequence.
ACCESSION   NZ_CP055329
VERSION     NZ_CP055329.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148604
            Assembly: GCF_013610655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T2-00409
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 69.7999234650958x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013610655.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 10:24:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,429
            CDSs (total)                      :: 6,308
            Genes (coding)                    :: 6,116
            CDSs (with protein)               :: 6,116
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 58 of 192
            Pseudo Genes (incomplete)         :: 154 of 192
            Pseudo Genes (internal stop)      :: 29 of 192
            Pseudo Genes (multiple problems)  :: 39 of 192
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055329.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00409"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:1134687"
                     /plasmid="pRHBSTW-00409_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(135..629)
                     /locus_tag="HV187_RS31910"
                     /old_locus_tag="HV187_31920"
     CDS             complement(135..629)
                     /locus_tag="HV187_RS31910"
                     /old_locus_tag="HV187_31920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_016240432.1"
                     /translation="MFVNPELHGKKRQEQLDENVRKATREHEEAKKNSRFTQVSPKGWE
                     RVRELLTDKQGVAALRLYSFLAEHIDPSCGAVVADQQFLADKMGVNRSTIIRWLNYLES
                     KNALVRIPVAGKVCAYALDPHEVWKGYNTSKDYSAFVTKTLVNNDGDIKRRIMSMFSSN
                     E"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 aaatatataa tatattttta atataaaaag caaatatatt ttgcgatatt atcaaaatat
       61 atttgctgag attttttatt ttattgtttt acattaaaaa acaataactt atatatttaa
      121 gtatcttgct ttggctattc attggaagaa aacatcgaca tgattcttct cttaatgtcg
      181 ccatcattgt taactaaagt ctttgtcaca aatgctgagt agtcttttga tgtgttatat
      241 cccttccaca cttcatgagg atctaatgca tatgcgcaga cctttcctgc aacggggatc
      301 ctaaccaatg cgttttttga ctctaagtaa tttagccacc ttattattgt cgatctattc
      361 acacccattt tatcggcaag aaattgctga tctgcaacaa ctgctccaca tgaaggatcg
      421 atatgttctg ctaaaaatga gtagagtcgt agcgccgcta ccccttgttt gtcagtgagg
      481 agctctctaa cacgttccca gcctttaggt gatacctggg tgaacctgga gttctttttc
      541 gcttcctcat gctctctagt ggcttttctg acgttctcat caagttgctc ttgccgtttt
      601 tttccgtgta actctggatt cacaaacatg acattttcgc ctttggtttt gatggtacat
      661 tgctgttcca aaaatgctac atcttgttgc atttacgcgt caaggtgttc cgtattgttg
      721 cacgcagggt gcaacaacgt tgttgcaccc tgcgtgcaac aggtcaaagg tatgtttccc
      781 agcaaaaaca ataagttatt atttttgcta tatctaatct ttaaagacct aacagcaaac
      841 atcaaaagtt aggtacctcc cacctttaat cgggcatttt tgagggtcaa ggttcacgta
      901 agtgaccgcg gagcggctcg accttgatgc tcaaaaatgc ctaacaagct tcgcggaggt
      961 ggttgtgatc acgcagtgag cgcaaccacc tgccagacgc atttgatttt agccattgcg
     1021 ggttccgggc gcagcgccgg accagtcacg tagcgctagc ggagtgtata ctggcttagg
     1081 tgtgtaagca gaaaatgtgg ggtgttggtt atcttgcgcc tccttcgttc attcgctcac
     1141 tacgttcggt catgaactgc ggcaggcact cttggctctt aaccttaatg gtttcgatac
     1201 caatcgctga tactgtcaac agcgagctac tcgagtttat ttacgtcgac agcgtattgt
     1261 tagatgatcg tgtgcagatg ttttgttaga ttatttattt gtaattataa aaaaacagcc
     1321 attcggctgt tttctttact gctatcgcca tgtaataaaa atacgcatat tattatcaca
     1381 acagccttat ttatttttat tttcataaag gctccatttt attattatgc cgaaatcgac
     1441 gat
//
