LOCUS       NZ_CP055324             2873 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00555 plasmid pRHBSTW-00555_10,
            complete sequence.
ACCESSION   NZ_CP055324
VERSION     NZ_CP055324.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148651
            Assembly: GCF_013608545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00555
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.8782588803698x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:17:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,253
            CDSs (total)                      :: 6,136
            Genes (coding)                    :: 5,958
            CDSs (with protein)               :: 5,958
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 69 of 178
            Pseudo Genes (incomplete)         :: 120 of 178
            Pseudo Genes (internal stop)      :: 29 of 178
            Pseudo Genes (multiple problems)  :: 35 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055324.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00555"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00555_10"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2813..2873,1..1046)
                     /gene="mobV"
                     /locus_tag="HV234_RS31110"
                     /old_locus_tag="HV234_31120"
     CDS             join(2813..2873,1..1046)
                     /gene="mobV"
                     /locus_tag="HV234_RS31110"
                     /old_locus_tag="HV234_31120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791318.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_181247285.1"
                     /translation="MYSIIRKQSHKKTAIQAVHNHNMRIVIEENVNSKKSNLNEILIGT
                     ENTRSDIMSYIKNNDIVIRNKDTVIFNEFVLTASPEFFFNNKNGSKKSRSEYDDNLKEW
                     VKTQISFLEKENYGKCVNAVLHLDESTPHIHALILPIKDGKLNNKSFWRGKNSYGRLQD
                     IYNTANSKFDLKRGEEKSKTLVDHTTLKDYRDLIRQDKDEENEFYNELSKNILDTPPRK
                     NFIGLEKKYSAEEVKEIATNTFKKLNRQRRRAKFRAKKSDEKADAATSEFYKKKKETSK
                     IEFFYNENQKQLEKISVENSTLKTHNLELKQSQSDLDLVRKFLPMELQSLVVRARELAS
                     GGDSGGTKHTAEPTPTADLTTYKRPKYK"
                     /name="mobV"
     gene            complement(1063..1806)
                     /locus_tag="HV234_RS31115"
                     /old_locus_tag="HV234_31125"
     CDS             complement(1063..1806)
                     /locus_tag="HV234_RS31115"
                     /old_locus_tag="HV234_31125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_154931427.1"
                     /translation="MTIQEVKKTRVRTKKENDKLTEYKMNPFVFEKELKIETKTRNLTV
                     GRNTELVRKDDSEESESYFTNIIQQKEVDKEEFIKLFTSQIKVYFDLTKTAYKIFLIVL
                     SLYQKEIGKDYVLLTCKKAQNIAKTLDFELSSPIFYRGIKELIEKKIIAKSVDKIVFYI
                     NPAIFFNGDRARFVTEVIKKKEEIEKNQLLQELEDTSKNKDFDVNETDDSVENLDLMIA
                     RLQKKKEELERKEKFGNWQDLENAS"
                     /name="hypothetical protein"
ORIGIN
        1 ataatatgag aatagttata gaagaaaatg taaattctaa aaaatcaaat ctgaatgaaa
       61 ttttaatcgg tactgaaaat actagatctg acataatgag ttatattaaa aataatgata
      121 ttgtcattag aaataaagac actgtaattt ttaatgaatt cgtacttact gcttcgcctg
      181 aatttttctt taacaataaa aatggttcaa agaaatctag atctgaatat gatgacaatt
      241 tgaaagagtg ggttaaaact caaatttctt ttttggaaaa agaaaattac gggaaatgtg
      301 ttaatgcagt tttgcattta gatgaaagta caccacacat acatgcatta attttaccta
      361 taaaagatgg aaaattaaac aataaatctt tttggcgagg taaaaattct tatggaagat
      421 tgcaggatat ttacaacact gcaaatagta aatttgattt aaaacgtggt gaagaaaaaa
      481 gtaaaacttt agttgatcat acaacattaa aagattacag agatttaatc agacaagata
      541 aagatgaaga aaacgaattt tataatgagc tttcaaaaaa tattttagat actccaccaa
      601 ggaaaaattt tattggttta gaaaaaaaat actctgctga agaagtaaaa gaaatagcaa
      661 cgaacacatt taaaaaacta aatagacaaa gaagaagagc aaaatttagg gctaaaaaat
      721 ctgatgaaaa agctgatgca gctacaagtg aattttataa aaaaaagaaa gagacatcga
      781 aaattgagtt tttttataat gaaaaccaga agcaattgga aaaaatctct gtggaaaatt
      841 ccacacttaa aacccataac ttggaactta agcaatcaca aagtgatcta gatctggtga
      901 gaaaattttt acctatggag ctgcaaagcc ttgtcgtcag agccagagaa ctggcctctg
      961 gcggcgattc tggcggtacg aagcatactg cagaacctac accaactgca gatctaacta
     1021 cctataaaag acctaaatac aaataaaagc ccatcagggc ttttatgatg cgttttccag
     1081 atcctgccaa tttccaaatt tttcttttcg ctctaactct tcttttttct tttgtaatct
     1141 tgcaatcatt aaatctaaat tttcaacgct atcatctgtt tcattaacat caaaatcttt
     1201 attttttgat gtatcttcta actcttgaag aagttgattt ttttctatct cttctttttt
     1261 ctttataact tcagttacaa atcttgctct atcaccattg aaaaaaattg ctgggtttat
     1321 gtagaaaaca attttatcta cagatttagc aataattttt ttctcaatta gttcttttat
     1381 acctctataa aaaattggtg acgaaagctc aaaatctaat gttttagcaa tattctgagc
     1441 tttcttgcat gtcaataaca cataatcttt acctatttct ttttgatata aagacaaaac
     1501 tataaggaaa attttatatg ctgtttttgt tagatcgaaa tatactttta tttgtgatgt
     1561 aaataatttt ataaattctt ctttatcaac ttctttttgt tgaataatat tagtaaaata
     1621 tgattctgat tcctctgaat catcttttct tactaattca gtatttcttc ctacagttaa
     1681 atttcttgtt tttgtttcaa tttttagttc tttttcaaaa acaaagggat tcatcttata
     1741 ttctgttaat ttatcatttt ctttttttgt tctaactctt gtttttttta cttcttgtat
     1801 tgtcataact tattcctctt taaatttaga tacttgatat tagtgttata accgatcctt
     1861 tgaacatgtc aataggaaac gataaaaaaa agtcgttttt gatagttaaa acgataaata
     1921 aaattctaga aattttagtg tgtttatcgt tttcataggg taaaacgata aagtttatta
     1981 ctctagagta taaaacttat tactctagag ttactttttt aaggatagag tataaaactt
     2041 tatacttttt gttttttaga aaaaagggag caaagccaga cgtatcaagg cttagagagt
     2101 agttttatga tttgagtttt tctgcaagaa ttaataaata taagatctaa gagtaaaacc
     2161 aaaatctaaa accgccctcg ccgggctggc caacccctcg aatccacata cagcaagctg
     2221 tacgtggact ctcacggtta aagtaactac aaaaacccgt cacagagcgt tacagagcgt
     2281 tctacagcta cataggggga acccctgaac ccgttccccc caccagaaca agactacttg
     2341 ttccgggtcc ccccacccca atgctagctg ctaccagcct gcatttaccc attcatatat
     2401 gcagtagaaa aagtttctga tcgtagactg cagtctacat atggaaactt tgcattttta
     2461 tattcaatct tttttaaagt gaaagtttac tttcgcttta agaaaagatt tagcaacgag
     2521 ctgcaaagcg aggcggtagc tatttgacag aatatattaa agtgataact ttaaatatat
     2581 acgttgagtt tgctttttcg gtctatcact cactagaccg agcttcgctc agtcgttcgt
     2641 tggggggcat agcccaccca agccccgaac ctgtttgcac aggttcaacg tcaaaacctt
     2701 acacagctgc acaggtggca tttaacgcca catgagaatg tatccgacat tctcataata
     2761 ttggatttat ccaaaaaaat gggggttgta taaataatat ggagaaataa aaatgtattc
     2821 aataatcaga aaacaatcac acaaaaaaac agctatacaa gctgtacata atc
//
