LOCUS       NZ_CP055322             4346 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00555 plasmid pRHBSTW-00555_8,
            complete sequence.
ACCESSION   NZ_CP055322
VERSION     NZ_CP055322.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148651
            Assembly: GCF_013608545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00555
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.8782588803698x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:17:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,253
            CDSs (total)                      :: 6,136
            Genes (coding)                    :: 5,958
            CDSs (with protein)               :: 5,958
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 69 of 178
            Pseudo Genes (incomplete)         :: 120 of 178
            Pseudo Genes (internal stop)      :: 29 of 178
            Pseudo Genes (multiple problems)  :: 35 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00555"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00555_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(66..1478)
                     /locus_tag="HV234_RS31065"
                     /old_locus_tag="HV234_31075"
     CDS             complement(66..1478)
                     /locus_tag="HV234_RS31065"
                     /old_locus_tag="HV234_31075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021197945.1"
                     /translation="MKEDQDEIQNLLAEREWEEFKKSSFGKFFTAKQMKEQELTYLNNK
                     ALDIVNEEDLFEVMSLIPQPFITNENIKKADILSVIYNNFIEETEIPLTLPIFSFFSFL
                     SAYCVKNNITYSMPLSEKKSHVNTWVTVLAPSGSAKTYSLGKIEDLIPIKPDGEKVVEQ
                     NFVKPSGPAKFIEEFSNLPDSRDGKSQYGFWIEDEVAQMFKQVEREGTPLSEIKEYFLK
                     AYDNKKLTRKSKKETIETKNLILVLFFINTFDSYVNNISSESMTDGLMRRFNLVYTEKD
                     KSRDFTDFPIYNEKKITDEILSDRFEDLFFNINPDVNYTFSENAFELYRKYFKIFWHRK
                     YKNILTDKENFYRTYMMQSWKFAVFYHLILKKEGTVIDGECLIYGMKISLLFANSMKKF
                     FDFKNKNISDSKREKQKDTLLKYADFLNSKPDATVRDFCRKFSIKKADAVSILKSLECS
                     GIKHSIFEVVSK"
                     /name="hypothetical protein"
     gene            complement(1958..3358)
                     /locus_tag="HV234_RS31070"
                     /old_locus_tag="HV234_31080"
     CDS             complement(1958..3358)
                     /locus_tag="HV234_RS31070"
                     /old_locus_tag="HV234_31080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181247283.1"
                     /translation="MYHKIIGGKKSKGQRNINKSIEYLLREKNPEQQKDIKILSTTTKQ
                     DLINFENYILDKNKSNPYVCGVLSFEEKTIPEELKTKIITEFEDILFVGIEPENRPPVL
                     WVEHNDKGRLELNYLTFNSLQDRRSYTVYLDKLDRALINNYSEIINYENNLSSPFEKKE
                     NKNTLVNNPKTNIPENKKDYLDKINTEILALIINEELDNREEAIKYIENLPGVKINRIR
                     KNQISIKCDFFDDKPIILKGDIYEEGRNYSDYRKQFNSTIERDPRIVRSEIDRNKAEFA
                     ERIEKRKSRNRSKFKNSQSKNKPENTKNIKGKNSNIEYRSINNNIDFTVNNNGFTDIND
                     NIFDMEKIKNESINSKYKTKTEEEERGRIARARDIEQINKQQQLIKRRIEEVRRTDSQL
                     SKSIETTNERVRRDRRSIQSIIYNARRHIRTLSAYFRKNEHKRYFEEKKRLEEEKQFNQ
                     KRRKKFIY"
                     /name="hypothetical protein"
     gene            complement(3359..3712)
                     /gene="mobC"
                     /locus_tag="HV234_RS31075"
                     /old_locus_tag="HV234_31085"
     CDS             complement(3359..3712)
                     /gene="mobC"
                     /locus_tag="HV234_RS31075"
                     /old_locus_tag="HV234_31085"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032458187.1"
                     /translation="MSKNYKDRYQRKHSLTLCFNDEEFQQVEELAEALRKPRATAVREL
                     ILNKEFKLKKEISKKENGDIYYELHKIGVNINQIARAINTDIDKFINQDFAEDFINLFE
                     EITNDIEKIKGKI"
                     /name="mobC"
     gene            complement(3705..4127)
                     /locus_tag="HV234_RS31080"
                     /old_locus_tag="HV234_31090"
     CDS             complement(3705..4127)
                     /locus_tag="HV234_RS31080"
                     /old_locus_tag="HV234_31090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021197946.1"
                     /translation="MDKAQKEVDLLAKKLELYYMKLLKKYNYRIFGFSCKTKKTEIDSS
                     EKIGLKDCMYVFKTESTIRKKDIVSKYFVLKNMKHQYVKNTYFNAYFCVDRKQYEKDED
                     YLNKIEKDFKERIKNKIERNEKTKDITYNERFKINE"
                     /name="hypothetical protein"
ORIGIN
        1 ttttttgtga ggggttgaac ttgccgaaaa actgttaaat ataaatacaa tattaaatcg
       61 gcaagttatt tgctcacaac ttcaaatatt gaatgtttta ttcctgagca ttcaagactt
      121 ttcaaaattg aaacagcatc tgctttttta atactgaatt ttctgcaaaa atctctaact
      181 gtcgcatctg gttttgaatt gagaaaatca gcatatttca ataaagtatc tttttgtttt
      241 tctctttttg agtctgatat atttttattt ttaaaatcaa aaaacttttt catactgttg
      301 gcaaataaca aagaaatttt cattccatat attaagcatt caccatcgat cactgttcct
      361 tcttttttca aaattaaatg gtagaataca gcaaatttcc agctttgcat catatatgtt
      421 ctataaaaat tttctttatc tgtgagaata tttttatatt ttctatgcca aaagatcttg
      481 aaatattttc tgtaaagttc aaaagcattt tctgaaaatg tataatttac atctggattg
      541 atattgaaaa ataaatcttc aaatctatca gacaaaattt cgtctgtgat ttttttctcg
      601 ttgtatatag ggaaatcggt aaaatctcgg ctcttatctt tttctgtata aacaagatta
      661 aatcttctca ttaaaccatc ggtcatagac tcggaagata tattattcac ataactatca
      721 aaagtgttta tgaaaaataa aaccaatatt agatttttag tttctattgt ctcttttttg
      781 ctttttcttg ttaatttttt attgtcatag gctttcaaga aatattcttt tatttctgaa
      841 agcggggttc cttctctttc aacttgtttg aacatttgag ccacttcgtc ttcaatccaa
      901 aagccatatt gagattttcc gtctctggag tctggaagat ttgaaaactc ttcaataaat
      961 tttgctgggc ctgacggctt gacaaaattt tgctcaacta ctttttctcc atcaggtttt
     1021 attggtatta agtcttctat ttttcctaat gaataggttt ttgctgatcc tgacggtgct
     1081 aaaaccgtaa cccaagtatt aacatgagat tttttttcag ataacggcat tgaatatgta
     1141 atattgtttt ttacacaata agcacttaaa aagctaaaaa atgaaaatat cggaagtgtt
     1201 aatggtattt ctgtttcttc aatgaaatta ttatatataa cactcaaaat atcagccttt
     1261 tttatatttt cattggtaat aaaaggctgt ggaattaatg acattacttc gaataagtct
     1321 tcttcgttta caatatctaa tgctttatta tttaaataag ttaattcctg ttccttcatc
     1381 tgttttgctg taaagaattt accaaatgaa gattttttaa attcttccca ttctctttca
     1441 gctaacaagt tttgtatttc gtcttgatct tctttcatat taaaaccctc taaatttatt
     1501 ttataatata tatatacaat ataaatttaa cttgtcaagt tttttaaaga aaaactccga
     1561 cacgtttttc actttttttc tgacagtccg acattctgac aaaatgactc aactcattga
     1621 tatataaggg tttttcgtta aaaatgggtt ctgacaaatc tccgacaaat ctccgacaaa
     1681 atgttctgac aggagaaaaa cgactatttt ttaagctggc acggagtgag tagagaaaga
     1741 aaatattcag ttttggggtt ttgtcttaaa aaaaatagct aaatctatga cacctacaag
     1801 ccgttttttt atgacaacta tgacaactat gacagaaaac cgtcctaccg gacaggtcaa
     1861 cccctcacaa gcaagaacca gcctgacggc tggtcctcac ttgttcgcgg ttttttcgta
     1921 ttgatctaat aaaatattaa gcattgattt tatatcctca atatataaat ttttttcttc
     1981 ttttttggtt aaattgtttt tcttcttcga gtcgtttttt ttcttcaaaa tatcttttgt
     2041 gttcgttttt tctaaaataa gctgaaagcg ttctaatgtg tcttcgagca ttatatataa
     2101 ttgattgtat cgatcttcga tctcttctaa ctctttcatt tgttgtttca atagactttg
     2161 agagctggct gtccgttcgt ctaacctcct caattcttcg tttaatgagt tgttgctgtt
     2221 tattaatttg ttcaatatct cttgctcttg caattcttcc tctttcttct tcttctgttt
     2281 ttgttttata ttttgaatta attgactcat tttttatttt ctccatatca aatatattat
     2341 cgttaatatc tgtaaaaccg ttgttgttga cagtaaaatc aatattattg ttaatagatc
     2401 tatattcaat attgctattt tttcctttaa tgttttttgt attctctggt ttatttttgc
     2461 tctgtgagtt tttaaacttt gatctatttc tgctttttct tttttcaatt ctttcagcaa
     2521 actctgcttt gtttctatcg atttcagatc taacaattct tggatctctt tcaattgttg
     2581 aattgaattg ttttctataa tcgctgtaat ttcttccctc ttcataaata tctcctttaa
     2641 gaataattgg tttatcgtca aaaaaatcac attttataga tatttgattt tttcgtattc
     2701 tattaatttt tactcccggt aaattctcaa tatattttat tgcttcttct ctgttgtcta
     2761 attcttcatt aattatcaaa gctaatattt cagtatttat tttatctaaa tagtcttttt
     2821 tattttctgg aatattagtt tttggattgt ttacgagtgt atttttattt tctttttttt
     2881 cgaatggact tgataaatta ttctcataat ttattatttc tgagtagttg ttaattaatg
     2941 ctctatctaa tttatctaaa taaactgtat atgatcttct atcttgaaga ctattaaaag
     3001 ttaaataatt taactctaat ctgcctttgt cattgtgttc cacccaaaga accgggggtc
     3061 tgttttccgg ttcgatacca acaaataata tatcttcaaa ttctgttatt attttagttt
     3121 ttaattcttc cggtattgtt ttttcttcaa aagataagac accacacaca tagggatttg
     3181 atttgttttt atctaaaata taattttcaa aattaattag atcttgtttt gtagttgttg
     3241 ataatatttt tatgtctttc tgttgttctg ggtttttctc cctcaataaa tactcaatgc
     3301 tcttatttat atttctctgg cctttgcttt ttttacctcc tattatttta tggtacattt
     3361 atatttttcc ttttattttt tctatatcat ttgttatttc ttcaaacaaa tttataaaat
     3421 cttcagcgaa atcttggtta ataaatttat caatatcagt atttattgct cttgctattt
     3481 gattgatatt aacccctatt ttgtgtagtt cgtaataaat atctccgttt tcttttttgg
     3541 atatttcttt tttgagttta aactctttat tgagaattaa ctctctaacg gctgttgcac
     3601 gcggttttct caatgcctca gccagttctt cgacttgttg aaactcttcg tcattgaagc
     3661 acagtgtgag actgtgcttt ctttggtatc tatctttata gtttttactc attaatttta
     3721 aatctctcat tgtatgttat atcttttgtt ttttcgtttc tttctatttt gtttttaatt
     3781 ctttctttaa aatctttttc tattttattt aaatagtctt cgtctttttc gtattgtttt
     3841 ctatcaacac aaaagtaagc attaaaatat gtatttttca catactggtg tttcatattt
     3901 tttaatacaa aatactttga tactatatct ttttttctga tcgtactttc tgttttgaaa
     3961 acatacatgc aatcttttaa gcctattttc tccgaactat ctatttctgt ttttttggtt
     4021 ttacatgaaa aaccaaaaat acgataatta tattttttca gtaatttcat ataatacaat
     4081 tctaattttt tagctaataa atctacttct ttctgtgctt tatccatatg ctaacctctg
     4141 ctctttttgg gttttaaggg gattgaaggg ggaacccctc acaagcgata atacgaagta
     4201 ttatctgagc ttgctgttat attatttccc tttttaatat acaattaata tatttaaaaa
     4261 taatgtcaat agctgtatat aaaataatca aatatatttt ttgttttatg aaaaccgtga
     4321 acaaataaag cccctttttt aggggc
//
