LOCUS       NZ_CP055320             5783 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00555 plasmid pRHBSTW-00555_6,
            complete sequence.
ACCESSION   NZ_CP055320
VERSION     NZ_CP055320.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148651
            Assembly: GCF_013608545.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T2-00555
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.8782588803698x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:17:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,253
            CDSs (total)                      :: 6,136
            Genes (coding)                    :: 5,958
            CDSs (with protein)               :: 5,958
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 69 of 178
            Pseudo Genes (incomplete)         :: 120 of 178
            Pseudo Genes (internal stop)      :: 29 of 178
            Pseudo Genes (multiple problems)  :: 35 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055320.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00555"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00555_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(4576..5783,1..1135))
                     /locus_tag="HV234_RS31000"
                     /old_locus_tag="HV234_31010"
     CDS             complement(join(4576..5783,1..1135))
                     /locus_tag="HV234_RS31000"
                     /old_locus_tag="HV234_31010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="WP_181486822.1"
                     /translation="MGGGFNYNGEGATGTGLDRDPYVRDSNGNAIGVKSGYHAESYGTS
                     SPALGPNGAIQITAGVIAVPGDKPRPDGGSGGGNTVNTGPAGQLLVMNKGQLGYWETRS
                     TGAGNNEHNTSVFVAVGPSEAEKTASAEKALKEKQQAEAAAKDFAAKTAAASATAEKER
                     QQAIAAATAAGQHQSVSDARNSLNNATSDVSRLKSAADSALQEAKAKRKAAIDAVPVAT
                     QAENKYQELQQKIKGLKLKNGEYGTEKWEIIGSNKEHDHWGYRFYPSGITKAQVDVAQS
                     DAVNKRNQATSLASQATAAEQDSLKATAAYNAAETRRQAAQAALNSAEQAAAAERKRQE
                     AEAAAAAAAEKKRQADAVAKAAEEARAAAEKARLMQERQAAADKLKSTDIQSVRGIPST
                     ASPAASPISWAVASLGGISLDSVTAGKAWTQIAEVMAKLRGIAGASLVGPVVATAVGLF
                     WSRDVGIGSDVVPGRDISGLMPGDALSLPDLATLIKAADSKTGVSMPVRGRIIVREGDY
                     LESQFVRTPVAGSVPVVRATLDKATGYWGYTLPAIQGVPGQTILVSPSDAPGVNAPLGL
                     AGPVPLPETIIHTGGQTTVPQGGTVTVSPVIGEVDFRDLILVFPPESGLKPLYVMPVSP
                     YGDATEKGEHSGRPYNPDKAGGPVQNLNWRSAVINKAGIDKVKLHTGRFGESAANKVMI
                     DRLEKILSGQLQVTDTDKRFYTHELRELERYRALGVPDNVKPDDKGVTWNNTHTATLED
                     YKINEKTDPLYTKDAEEAEYKAELKNK"
                     /name="S-type pyocin domain-containing protein"
     gene            complement(2251..2673)
                     /locus_tag="HV234_RS31005"
                     /old_locus_tag="HV234_31015"
     CDS             complement(2251..2673)
                     /locus_tag="HV234_RS31005"
                     /old_locus_tag="HV234_31015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064148699.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2673..2864)
                     /locus_tag="HV234_RS31010"
                     /old_locus_tag="HV234_31020"
     CDS             complement(2673..2864)
                     /locus_tag="HV234_RS31010"
                     /old_locus_tag="HV234_31020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3280..3394
                     /locus_tag="HV234_RS31165"
                     /pseudo=""
     CDS             3280..3394
                     /locus_tag="HV234_RS31165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3600..3755)
                     /locus_tag="HV234_RS31015"
                     /old_locus_tag="HV234_31025"
     CDS             complement(3600..3755)
                     /locus_tag="HV234_RS31015"
                     /old_locus_tag="HV234_31025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(3863..4120)
                     /locus_tag="HV234_RS31020"
                     /old_locus_tag="HV234_31030"
     CDS             complement(3863..4120)
                     /locus_tag="HV234_RS31020"
                     /old_locus_tag="HV234_31030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015366249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_010904244.1"
                     /translation="MANKTLADYTEQEFIEFIEKIKKADFATESEHDEAIYEFSQLTEH
                     PDGWDLIYHPQAGADNSPAGVVKTVKEWRAANGKPGFKKS"
                     /name="bacteriocin immunity protein"
     gene            complement(4302..4565)
                     /locus_tag="HV234_RS31025"
                     /old_locus_tag="HV234_31035"
     CDS             complement(4302..4565)
                     /locus_tag="HV234_RS31025"
                     /old_locus_tag="HV234_31035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064148700.1"
                     /translation="MSNPIEDIIKNESVSDVLRYFGPGRDISKIDRMYVSYKFEVISEG
                     VLLSEYKKLFDNGELKYDSNKNVIKGPNWKEPAFVTQKKYGI"
                     /name="hypothetical protein"
     Typing          2986..3394
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gacgctcctg catcagcctg gctttttccg ctgccgcacg cgcttcctct gctgcttttg
       61 ctactgcgtc tgcctgacgt tttttctcag cagcagctgc tgcagccgcc tcagcttcct
      121 gccgcttacg ttcagcagca gcagcctgtt cagcagaatt taacgccgcc tgagcagcct
      181 gacggcgcgt ttctgccgca ttataggcag ctgtagcttt caggctgtcc tgttctgctg
      241 ctgttgcctg actggcaaga ctggtagcct gatttcgctt attcacagca tcgctctgcg
      301 ccacatcaac ctgagctttg gtaattccgg atggataaaa cctgtatccc cagtgatcat
      361 gctccttatt agagccaatt atttcccatt tttccgtacc atactcacca tttttcagct
      421 tcaggccttt aatcttctgc tgcagctcct gatatttatt ttccgcctgg gttgcaacag
      481 gtacagcatc aatagcagcc ttccgttttg cctttgcctc ctgcagtgca ctgtctgcag
      541 ctgatttcag acgagacaca tctgacgtcg cgttattcag gctattacgg gcatcagaaa
      601 ctgactgatg ctgacccgct gcagttgcag cagcaattgc ctgctggcgt tctttttccg
      661 ccgtggctga tgcggcagca gttttagccg caaagtcttt tgctgctgct tctgcctgct
      721 gtttttcctt taacgccttc tctgcagaag cagttttttc tgcctcagaa ggacctacgg
      781 caacaaatac actcgtatta tgctcattgt tacccgcgcc cgtagaacga gtttcccagt
      841 atccaagctg acctttattc atcaccagga gctgtcctgc cggtccggtg ttaacagtat
      901 tcccaccacc actaccaccg tcaggtcggg gcttatctcc aggcacagca ataaccccag
      961 cagtaatctg aatagcgcca ttaggcccta aggctggact tgaggtccca taggactccg
     1021 cgtgatagcc tgatttaaca ccaatagcat taccattgct gtcgcgaaca tatggatcac
     1081 gatccaatcc ggtgccagta gcaccttccc cgttatagtt aaatccacca cccataactc
     1141 tctcctttgt tgttattttt atcgttttcc tgcttgtcag aatctggtgt gcggtttatc
     1201 gccttcgaac ctgtatccgg aggctgttat tagttctctg ccacaccaag tactgtatat
     1261 aaatacatgt gtttatatac agtacttgaa tgctactcct gacatgagaa ctctgtcaac
     1321 acgcggagag atacaattat cttttgaatt ttcattaagt taagcgtaat agaaatgaca
     1381 aaacccagac aggacgcggc tcggcagaga aaaaaaatta acgatatgtg ctcttaaaaa
     1441 gggtgtgact gacggacaga gacctgtaca aaaagtcttg gtgtagaggg tattccgcca
     1501 cccgagcgcc cccatttacc ataatccctt aattgtacgc accgctaaaa cggcctcagg
     1561 acgtttctga cgggaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac
     1621 gcgcaggctc tgtttttacg gcatgcatga gtatgcgcgc aagtgcatag cgcggaaatt
     1681 cacgccattc agccagggta agtcctgttt ccggcgttct tctgatccga aaacggtgaa
     1741 cggggacgca atttattgcg tggccgtgag gcgttgccgg cgcagccgga aaaccggttt
     1801 tcagcacgtt caggaatcgg gttcgtcgac ggccggcaat cacgttatgg caagtcgccg
     1861 gacgactcac cacacgattc accggcgatt cgaatcgtga attgaatcaa gagtcaccat
     1921 gaacaaaaaa cgcccaaatt gatgaattga atcaagagtc gattcatcat aacaacatga
     1981 taataaagcg tgattcacat atgaatcgtg ggcaattcgt aaacgattcg ccatagagtc
     2041 gtccacgact tggatctgca ctttgtaaca catcactgca cattaatttt tcattaacca
     2101 attgttttaa ataaagaatc tgcaaaattt taattttgcg tagtgtgtgg gtatttttag
     2161 tggtgaggag cgttcagaga tctggcgatt ttttggaagg ttcaggaact ccccggaatg
     2221 gccggagagg aaaactgaag catgatgggg tcaggatttt ttccagaatt tccaccacgg
     2281 ggaatggggt ggtgaagaag gttcgtgctt gtgctccaga agctgcatag cctgacgaag
     2341 ggaatcaata tgctcatctc gagccttaac agccccttta agaccatcgt tttcagcttc
     2401 aagtaaagcg attctatgtt gtaaatcatg taaaccaacg ctgttaatac ctgtagattc
     2461 tgcgtcactt tttacatcat attttacagt atcgattaca ccatcaagag aaccaaacac
     2521 tctaataagc tcagaagtat caataacttt aatagccttc ccgtgcttta caacagaggt
     2581 gtaacttaac ttcccactgt taatcatctt gtaaattgta ggacgactaa ccttaacaag
     2641 ccgtgccgct tcactaatag acactctggc catcagttac catcctgtaa ttcatccagt
     2701 ctcgaggaca gtgtacggaa aagatgctcc gcatcatcgt ggagtttctc acaaaggtcg
     2761 gcctcgacat cgagatcaag ctcgttaagc ttctccagca ggagcagaga ctgattctgg
     2821 atgaatttag ccatgttcag cgctgttttc tgttgtttcg tcataagagg cctcagagcg
     2881 tcagtgctca gtgtagggat gttctctttt ccgcttcctc gctcactcag tcgctacgct
     2941 cctgccgttc ggctgcggcg agcggcagga gcatgcgtat aaaagctcga gaaaaccgag
     3001 cgatcgcgtg atggggagaa ggagaggccg cacagcggcc gtttttccat aggctccgcc
     3061 cccctgacaa gcatcacaga atttgacgct caaatcagtg gtggcgaaac ccgacaggac
     3121 tataaagata ccaggcgttt ccccctgatg gctccctcgt gcgctctcct gttcctgcca
     3181 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt atctcattcc acgcctgaca
     3241 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     3301 tccgactgct gcgccttgat ccggtaacta tcatcttgag tccaacccgg taagacatgc
     3361 aaaagcacca ctggcagcag ccactggtaa ctgatttaga ggagttagtc ttgaagttat
     3421 gcgccggtta aggctaaact gaaagaacaa gttttggtta ctgtgctcct cataagccag
     3481 ttacctcggt tcaaagagcg ccaactcttg gaaccttcga aaaaccgccc gccaaggcgg
     3541 ttttttcgtt tacagagcaa gagattacga cgatccaaat gggatctcaa gaagatcctt
     3601 tacggattgc cgttcgtacc gctatcaagc gtgactccgg tcaccgtcga tgaaggtgaa
     3661 acagccccac ctccggtgtc acgaacatta ttgacctgac atgccgccag aaccacagaa
     3721 acggtaacaa cccccaggaa aagtaatttt ttcatatatc accttttatt tttattattc
     3781 taagcaaatg gatacctagt aattattatt caaatccaga gttaaaaatg tgcggattca
     3841 tcacttgaac ccgcacattc aatcacgatt ttttaaaacc tggcttaccg ttagctgctc
     3901 gccactcttt tactgttttt acaacaccag caggagagtt atcggctcct gcttgaggat
     3961 gataaataag atcccaacca tctggatgtt cagtcaactg gctgaactca taaatggcct
     4021 catcatgctc agactcagta gcaaagtctg cttttttaat tttttcgata aactcaataa
     4081 attcctgctc tgtatagtca gccaaagttt tattagccat ttcagttccc attatggatt
     4141 tcaatatggc gtttaggttt taatgcactc agcttacccc agatattgtt gacctgacat
     4201 gctgccagta tcatcgaaac cacgaccact caaaaaagaa acccttttca tatgtgagct
     4261 ttaatcaaag ccctcttttc aaagggcttc atatgcacac tttaaatgcc gtattttttt
     4321 tgtgttacaa aagcaggctc tttccagtta ggccccttaa taacattttt attagagtca
     4381 tactttagtt ctccattgtc gaataacttt ttgtattcag ataaaagaac gccttcagag
     4441 atcacttcaa acttatatga aacatacata cggtcaattt ttgaaatgtc gcgaccagga
     4501 ccaaagtagc gaagcacatc acttaccgat tcatttttaa tgatgtcttc aatcggatta
     4561 ctcattctct ccccctcatt tattttttag ctctgcttta tactctgcct cttcagcatc
     4621 ctttgtatag agcggatcag ttttttcatt aattttataa tcttccagcg ttgctgtatg
     4681 agtgttattc cacgtaacac ctttatcatc tggcttcaca ttatcaggga cccctaaagc
     4741 acgataacgt tccagctctc tgagttcatg ggtgtaaaac cgcttgtccg tatcagttac
     4801 ctgtaactga ccgctcagga ttttctccag acggtcgatc attactttgt ttgctgctga
     4861 ttcaccaaag cgaccggtat gtagcttcac cttatcaatc ccggccttgt tgatgactgc
     4921 actacgccag ttaagattct gtacaggacc acctgcctta tccggattat aaggtcttcc
     4981 gctgtgctcg cctttttctg ttgcatctcc ataagggcta actggcatca catataacgg
     5041 ctttaaccct gactcaggag ggaaaacaag gataagatct cgaaaatcaa cttcaccaat
     5101 aacaggagat actgtcacag tccccccctg agggaccgta gtttgcccac cggtatgtat
     5161 aatagtttca ggcaaaggaa ccggcccagc aagtcccaga ggagcattaa cgcccggcgc
     5221 atctgacgga cttaccagta ttgtctgtcc gggcacgccc tgtatcgccg gcaacgtata
     5281 tccccagtaa ccagtagctt tatccagagt agcccgaaca accggaacac taccggcaac
     5341 aggtgttcga acgaactgag actccagata atcgccttca cgcacgataa tccggcctcg
     5401 aaccggcata ctgacacccg ttttactgtc agcagcttta atcaaagtgg ccagatcagg
     5461 taatgacagt gcatcacccg gcatcagccc gctgatgtca cgtccgggca ccacatcact
     5521 gccaatacca acatcacgtg accaaaacag ccctacagca gttgccacca cgggaccaac
     5581 aagactcgca ccggcaatac ctcgcagttt agccatcacc tcagcaatct gcgtccaggc
     5641 tttccctgca gtaacactat ccagcgatat accaccaagt gatgcaacgg cccaggaaat
     5701 gggtgacgct gcaggcgatg ccgtagacgg gatcccgcga acagactgaa tatctgtgga
     5761 tttcagctta tctgctgctg cct
//
