LOCUS       NZ_CP055314             3273 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00866 plasmid unnamed.
ACCESSION   NZ_CP055314
VERSION     NZ_CP055314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148723
            Assembly: GCF_013606065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00866
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 119.112397777653x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:26:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,611
            CDSs (total)                      :: 6,495
            Genes (coding)                    :: 6,318
            CDSs (with protein)               :: 6,318
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 59 of 177
            Pseudo Genes (incomplete)         :: 136 of 177
            Pseudo Genes (internal stop)      :: 30 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055314.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00866"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2058152"
                     /plasmid="unnamed"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(48..>650)
                     /locus_tag="HV306_RS32805"
                     /old_locus_tag="HV306_32815"
                     /pseudo=""
     CDS             complement(48..>650)
                     /locus_tag="HV306_RS32805"
                     /old_locus_tag="HV306_32815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            <649..990
                     /locus_tag="HV306_RS32810"
                     /old_locus_tag="HV306_32820"
                     /pseudo=""
     CDS             <649..990
                     /locus_tag="HV306_RS32810"
                     /old_locus_tag="HV306_32820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1738..2160)
                     /locus_tag="HV306_RS32815"
                     /old_locus_tag="HV306_32825"
     CDS             complement(1738..2160)
                     /locus_tag="HV306_RS32815"
                     /old_locus_tag="HV306_32825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2160..2351)
                     /locus_tag="HV306_RS32820"
                     /old_locus_tag="HV306_32830"
     CDS             complement(2160..2351)
                     /locus_tag="HV306_RS32820"
                     /old_locus_tag="HV306_32830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2756..2917
                     /locus_tag="HV306_RS33385"
     CDS             2756..2917
                     /locus_tag="HV306_RS33385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            complement(3075..3230)
                     /locus_tag="HV306_RS32825"
                     /old_locus_tag="HV306_32835"
     CDS             complement(3075..3230)
                     /locus_tag="HV306_RS32825"
                     /old_locus_tag="HV306_32835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     Typing          2400..2866
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.979"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 cagtaaattc ttcttccgta aaatctgaaa ttttattacc agccatttta ttttccttta
       61 tgtgtctcaa tatgattttt aggtgttagc gccttaatgt tatcgacatc cataacagcc
      121 cctccttgag atatttcttt ttcgtggtgt aattcaattt taacacgttt ccctactgat
      181 tctaaaaagt ccgcagtagg tgcccttcca gctttcatct gattaatatc agcttcacta
      241 aattgcttgc tcagtgcaga atcgtcagca accgctttcc agaacgcccg acggaaagaa
      301 tcaaaactac cgaaagtctt cccgcgtaat ttatccgcaa tctgggaagg aacaggagca
      361 ccatccccgg tactggcccc ccccatccag ttattgccaa cgttctgccc tttgccgctc
      421 actgtaccgg gcatgttacg agggctacgg tacatcacat acagcggttt taggccggat
      481 tccggaggaa acaccagaat aatatcgtca aaatcgaggt catccgctac cggcgtcacg
      541 gttttatctg tagcctgtgg cgccgaaatc tgatcaccgg tatgcagaat acgctccggt
      601 agtggtaccg gcccgcttag cgtcagtggt ccgtttgcac caggtgcgtc aagtgcacta
      661 gatttcacca ggatggcgct taatcaggaa aaatacctcg atgactttac atggaaagaa
      721 ctgaacgacg ttatcaactt catacgcaaa gcagaaggta gtgcatctga ccacagctat
      781 gcactccagt tactggagat taatttcaag gcaaacccac tggatctcat ctatcaccct
      841 gactgctggt tcaatgatga agcattgttt catgcccggc tgactaccga agaaattggt
      901 ggctacttaa tgaaaaaatc aggtcgatgg ctcaacgatg caccggatat ccaactggtg
      961 tatgccatcc cccaggatgt ttatgattaa ccaagtcacc atttaccata atcccttaat
     1021 tgtacgcacc gctaaaacgg cctcaggacg tttctgacgg gaaaccggta aaacaggcaa
     1081 caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta
     1141 tgcgcgcaag tgcatagcgc ggaaattcac gccattcagc cagggtaagt cctgtttccg
     1201 gcgttcttct gatccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaggcg
     1261 ttgccggcgc agccggaaaa ccggttttca gcacgttcag gaatcgggtt cgtcgacggc
     1321 cggcaatcac gttatggcaa gtcgccggac gactcaccac acgattcacc ggcgattcga
     1381 atcgtgaatt gaatcaagag tcaccatgaa caaaaaacgc ccaaattgat gaattgaatc
     1441 aagagtcgat tcatcataac aacatgataa taaagcgtga ttcacatatg aatcgtgggc
     1501 aattcgtaaa cgattcgcca tagagtcgtc cacgacttgg atctgcactt tgtaacacat
     1561 cactgcacat taatttttca ttaaccaatt gttttaaata aggaatctgc aaaattttaa
     1621 ttttgcgtag tgtgtgggta tttttagtcg tgaggagcgt tcagagatct ggcgattttt
     1681 gggaaggttc aggaactccc tgggatggcc ggagaggaaa actcaagcat ggcaggatca
     1741 ggattttttc cagaatttcc accacgggga atggggtggt gaagaaggct cgcgcttgtg
     1801 ctccagaagc tgcatagcct gacgaaggga atcaatatgc tcatctcgag ccttaacagc
     1861 ccctttaaga ccatcgtttt cagcttcaag tagagcgatt ctatgttgta aatcatgtaa
     1921 accaacgctg ttaatacttg tagattctgc gtcacttttt acatcatatt ttacagtatc
     1981 gatgacacca tcaagagaac caaacactct aataagctct gaagtatcaa taactttaat
     2041 agccttcccg tgctttacaa cagaggtgta acttaacttc ccactgttaa tcatcttgta
     2101 aattgtggga cggctaacct taacaagccg tgccgcttca ctaatagaca ctctggccat
     2161 caattaccat cctgtaattc atccagtctc gaggacagtg tacggaaaag atgctccgca
     2221 tcatcgtgga gtttctcaca aaggtcggcc tcaacatcga gatcaagctc gttaagcttc
     2281 tccagcagga gcagagactg attctggatg aatttagcca tgttcagcgc tgttttctgt
     2341 tgtttcgtca taaagggcct cagagcgtca gtgctcaatg tcagacagat ctcttttccg
     2401 cttcctcgct cactcagtcg ctacgctcct gccgttcggc tgcggcgagc ggtcgcaacg
     2461 cgcacataac gacatagagc agacagctcg gagagggaga aggcagcaca gcggccgttt
     2521 ttccataggc tccgcccccc tgacaagcat cacagaattt gacgctcaaa tcagtggtgg
     2581 cgaaacccga caggactata aagataccag gcgtttcccc ctgatggctc cctcgtgcgc
     2641 tctcctgttc ctgccattcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     2701 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     2761 cgaacccccc gttcagtccg actgctgcgt cttatccggt aactatcatc ttgagtccaa
     2821 cccggaaaga cacgacaaag cgccactggc agcagccact ggtaacggat ttagaggagt
     2881 tggtcttgaa gtcatgcgcc tgttaaggct aaactgaaag aacaagtttt ggtgactgcg
     2941 ctcctctaag ccagttacct tggttcaatc acctgcaagt gtttgaacct tcggaaaaac
     3001 cgccctgcaa ggcggttttt tcgtttacag agcaagagat tacgacgatc caaatgggat
     3061 ctcaagaaga tcctttacgg attgccgttc gtaccgctat caagcgtgac tccggtcacc
     3121 gtcgatgaag gtgaaacagc cccacctccg gtgtctcgaa cattattgac ctgacatgct
     3181 gccagaacca cagaaacggt aacaacccct aggaaaagta attttttcat atatcacctt
     3241 ttatttttat tattctaggc aaatggatat cta
//
