LOCUS       NZ_CP055313             3844 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella grimontii strain RHBSTW-00866 plasmid pRHBSTW-00866_5,
            complete sequence.
ACCESSION   NZ_CP055313
VERSION     NZ_CP055313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15148723
            Assembly: GCF_013606065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T3-00866
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 119.112397777653x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:26:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,611
            CDSs (total)                      :: 6,495
            Genes (coding)                    :: 6,318
            CDSs (with protein)               :: 6,318
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 59 of 177
            Pseudo Genes (incomplete)         :: 136 of 177
            Pseudo Genes (internal stop)      :: 30 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00866"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:2058152"
                     /plasmid="pRHBSTW-00866_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(185..1609)
                     /locus_tag="HV306_RS32790"
                     /old_locus_tag="HV306_32800"
     CDS             complement(185..1609)
                     /locus_tag="HV306_RS32790"
                     /old_locus_tag="HV306_32800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136070838.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTKKPEEQKFVEVLSQTNRSDI
                     ENFNEYIKDKNFKNPFITGVLSFEEKDIDPELKTKIMNDFEDMMFSGIEPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVQEHK
                     ARNTLVNTLNNKIPEEKKAVVEKLQEEILSKIITEEINNREELISFIQSKNIIINRVRE
                     NAISIKFDKADKPISLKGDIYEQNRDYKTYRTESTIDHRADQQYVENQLRHHRENFDTE
                     LKKRHSRNRTRFKPTQSKDQKNVEYRVKKANDSKNVRSEIPDNNNVPVRNEPDIDFFHN
                     NKTIEDVSNEQNFTNDKTKPREEEPAKFDLSREIETANTQQQFIRSRVEEVRRNQQQFR
                     KSLDRTQERSGVCRRSLHSIIHNIRRYVRTFSIHTRQNERRQKIEHRQKIFLEKLKTQQ
                     EKNNQRSVVRSRFKPY"
                     /name="hypothetical protein"
     gene            complement(1599..1946)
                     /gene="mobC"
                     /locus_tag="HV306_RS32795"
                     /old_locus_tag="HV306_32805"
     CDS             complement(1599..1946)
                     /gene="mobC"
                     /locus_tag="HV306_RS32795"
                     /old_locus_tag="HV306_32805"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048982277.1"
                     /translation="MSYRERYQRKNFISLCLSDEELSEIENIADRLNMKRAAAAREILV
                     TNSKRLKSQIKKNDNSEILFLYSKISNNINQIAKKMNTNLDKFLSGNGEEFSLLIEEIF
                     EDLERLKNNDT"
                     /name="mobC"
     gene            complement(2228..3631)
                     /locus_tag="HV306_RS32800"
                     /old_locus_tag="HV306_32810"
     CDS             complement(2228..3631)
                     /locus_tag="HV306_RS32800"
                     /old_locus_tag="HV306_32810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127713719.1"
                     /translation="MSEKSIEEMLAEKQIKEFNESKFGQFFNNKKLKEQELTYLNNKAL
                     DIVNEEDLFEIMSLIPQPFITDENKNVDVLSLIYNNFLEHTEIPLTLPIFSFLSFLSAF
                     CVKNEITYTIPLSDEKSPLDTWITILAPSGSGKSFSNSQMKKVIPTGIDGKKVIEPNFV
                     KPNGPAKFIEELSNLPTTKNGENQFGFWIEDEVSQMFKQVEQIGSPLSEIKEYLLKAYD
                     HEKLTRKSKKESIETKGIILTLLFINTFESYANNISNESMTDGLMRRFNLIYTEKDIER
                     DFTDYYLYNLKKIRDDRLQDEIADLFFSIKPDQNFTFSDNCFKLYGKYFKIFWHRKYKN
                     ILTDKENFYRTYMMQSWKFAVFYHLILKREGTEIQGDCLIYGMKISLLFANSMKKFFDF
                     KIRKNVPDKKIEKIKNDLTKYIVFLENNPEATLRDFCRKYNIRKIDANSILKSLQCANI
                     QHKLIEELE"
                     /name="hypothetical protein"
ORIGIN
        1 gagtttttga aatattttga aacatattta ttttttacct gtcataaaaa ataaaaaaac
       61 aaacgacaaa accgcctcgc cggcaggcca acccctcaca agcaaaaacc ccactatgtg
      121 gggctttcac ttgttcacgg ttaaggtttt gtcatagtgg gttagaagag tgttcagaac
      181 ggttctaata cggtttaaat cgacttctga cgacacttct ttgattgttt ttttcttgct
      241 gggttttgag cttttcaagg aagatttttt gtctgtgttc gattttttgt cttctttcat
      301 tctgtcttgt atggattgaa aacgttctga cgtatcttct aatattgtga ataattgaat
      361 gtagagatct tctacatact ccagatcgtt cctgagttct atcaagcgac tttctgaatt
      421 gctgttgatt tcttctaacc tcttcaactc ttgaacgaat gaattgttgt tgtgtattag
      481 ctgtttcaat ttctcttgat agatcaaatt ttgctggttc ttcttctctt ggttttgttt
      541 tatcatttgt gaaattttgc tcattgctta catcctcgat agttttatta ttatgaaaaa
      601 aatcaatatc aggttcattc ctaacaggaa cattattatt gtctggtatt tcagatctaa
      661 catttttact gtcatttgct tttttaactc tgtattcaac atttttctga tctttgctct
      721 gtgttggctt aaaacgagtt ctatttctgc tgtgtctttt tttaagctct gtgtcaaaat
      781 tttctctatg gtgtctgagt tggttttcga catactgctg atctgctctg tgatcgattg
      841 ttgattctgt tctataagtt ttgtaatctc tgttctgttc atatatatct ccttttaagc
      901 tgataggctt atcagcctta tcaaatttta ttgatatagc attttctcta accctgttta
      961 ttattatgtt tttagattga ataaatgata ttaattcttc tctgttgttt atttcttctg
     1021 tgattatttt cgataaaatc tcttcttgta atttctcaac aactgccttt ttttcttctg
     1081 gtattttatt atttaaagtg ttaacgaggg tatttcttgc tttatgttcc tgaacatcca
     1141 aaacagaact gatattattt tcatagttta ttttttcgca aaatgagttg aataattttt
     1201 tatctgacaa atgataataa cattgaaaat gttttttatt cattagggaa ttaaaagttg
     1261 tatagtttaa ttcaagtctg tttttatctt tgtgttgtat ccaaatgacc ggaggtctgt
     1321 tttctggttc tattcctgaa aacatcatat cttcaaaatc attcattatt tttgttttta
     1381 attctggatc gatgtctttt tcttcaaaag ataaaacacc agttatgaag gggtttttaa
     1441 agtttttgtc ttttatgtat tcgttgaaat tttcaatatc agatctgttt gtttgagata
     1501 aaacttcaac aaatttctgt tcttctggtt ttttagttct taataaataa tcaagtgaat
     1561 ttttaacact tcgctgattt ttctttcctc cgatatgttt atgtatcatt attttttaac
     1621 ctctctaaat cttcgaatat ttcttcaatg agtagtgaaa attcttctcc gtttcctgat
     1681 aagaatttat caagattagt gttcatcttt tttgcgatct gatttatatt gtttgagatt
     1741 ttagaatata aaaacaatat ttcagaatta tcatttttct ttatttgtga ttttagtctc
     1801 tttgagtttg tgactaatat ttcacgagct gcggctgctc gtttcatatt taacctatca
     1861 gcaatatttt caatctctga taattcttca tcactcaaac acaatgagat aaaatttttt
     1921 ctctggtatc gttcacggta actcaaattt aactcctttg ggtttaaggt tttgattttg
     1981 atttggtttt taggggattg aagggggaac ccctcacaag taaaggaata cgaagtatta
     2041 ctctaacttg ctatctatta catagataac aaattaaaac attaagtcaa gggcaaaact
     2101 gaatataatt tgacctttta taaaaccgtg agaaaataaa aaagcccgtc agggctttat
     2161 tatttttgag gggttgaaca agcgaaaata tatttgaata tggcacaatt taaaattcgc
     2221 ttgttaatta ctccagttcc tcaataagtt tgtgttgaat attagcacat tgaagacttt
     2281 ttaatataga attagcatct atttttctga tgttatattt tctgcaaaaa tctctcaatg
     2341 tagcttctgg gttattttct aaaaaaacaa tatattttgt taaatcattt tttatttttt
     2401 ctattttttt atctggaacg ttttttctta ttttaaagtc aaaaaacttt ttcatactgt
     2461 tagcaaaaag taatgatatt ttcatcccat atattaaaca atcaccctga atttcagttc
     2521 cttctctttt taaaattaaa tgataaaata cagcaaactt ccagctttgc atcatataag
     2581 ttctataaaa attttcttta tctgtgagta tatttttata ttttctatgc caaaaaattt
     2641 taaaatactt gccatataat ttaaaacaat tatcagaaaa ggtgaaattt tgatctggtt
     2701 ttatagaaaa aaacaaatca gcaatctcat cttgtaatct atcatctctt attttcttta
     2761 aattatataa ataataatca gtaaaatctc gttcaatatc tttttctgtg tatattaaat
     2821 taaatctacg cattaatccg tctgtcatag attcatttga aatgttattc gcataacttt
     2881 caaatgtatt tataaataat aaagttaaaa taatgccttt ggtttctatt gattcttttt
     2941 tgctttttct ggttaatttt tcatggtcat atgcttttaa taaatattct ttaatttcag
     3001 agagcggaga tccaatttgt tctacttgtt tgaacatttg tgaaacttca tcttcaatcc
     3061 aaaatccgaa ttgattttcg ccattttttg ttgtgggtaa atttgaaagt tcttcgataa
     3121 attttgcggg accgttaggt tttacaaagt tcggttcaat aacttttttt ccatctattc
     3181 cagtaggaat aacctttttc atttgtgaat ttgagaatga ttttccgcta cctgatgggg
     3241 ctaaaattgt gatccaagta tcaagcggcg atttttcgtc gctcaatgga attgtatatg
     3301 tgatttcatt tttaacacaa aaagcactta aaaaacttaa aaacgaaaag atgggaagtg
     3361 ttaatggtat ttctgtatgt tctaaaaaat tattatatat aagacttaat acatctacat
     3421 ttttattttc atcagtaata aaaggttgag gtattaatga cattatttcg aataagtctt
     3481 cttcatttac gatgtctaat gctttattgt taaggtatgt tagttcttgt tcttttagtt
     3541 ttttattatt aaaaaactga ccaaattttg attcgttaaa ttcttttatt tgcttttcag
     3601 ctaacatttc ttcaattgat ttttcactca taattaattc tcctattttg attatataaa
     3661 atatatataa tacattttta aatgtgtcaa atatttttta aaaaaaatat gacttttttt
     3721 tcgcgcttct ttttgacact atgacacttt gacaaatcaa taaagccttt gtttttaaag
     3781 gaaaaaccat tttttttggt tttgacaaat ctttgacaaa gttttgacaa aatcaaatca
     3841 cttg
//
