LOCUS       NZ_CP055300             5596 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP55 plasmid pKP55_6, complete
            sequence.
ACCESSION   NZ_CP055300
VERSION     NZ_CP055300.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15234688
            Assembly: GCF_018336835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Li,X.
  TITLE     Ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella
            pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Quan,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2020) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-JUN-2020
            Assembly Method                   :: Unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 11:30:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,982
            CDSs (total)                      :: 5,860
            Genes (coding)                    :: 5,681
            CDSs (with protein)               :: 5,681
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 68 of 179
            Pseudo Genes (incomplete)         :: 118 of 179
            Pseudo Genes (internal stop)      :: 25 of 179
            Pseudo Genes (multiple problems)  :: 32 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP55"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP55_6"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="30.3 N 120.2 E"
                     /collection_date="2020-04"
                     /collected_by="Xi Li"
     gene            complement(join(5491..5596,1..245))
                     /locus_tag="HUW33_RS29640"
                     /old_locus_tag="HUW33_29635"
     CDS             complement(join(5491..5596,1..245))
                     /locus_tag="HUW33_RS29640"
                     /old_locus_tag="HUW33_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(311..856)
                     /locus_tag="HUW33_RS29645"
                     /old_locus_tag="HUW33_29640"
     CDS             complement(311..856)
                     /locus_tag="HUW33_RS29645"
                     /old_locus_tag="HUW33_29640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(882..1208)
                     /locus_tag="HUW33_RS29650"
                     /old_locus_tag="HUW33_29645"
     CDS             complement(882..1208)
                     /locus_tag="HUW33_RS29650"
                     /old_locus_tag="HUW33_29645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1566..1724)
                     /locus_tag="HUW33_RS30220"
     CDS             complement(1566..1724)
                     /locus_tag="HUW33_RS30220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2364..2540
                     /locus_tag="HUW33_RS29655"
                     /old_locus_tag="HUW33_29650"
     CDS             2364..2540
                     /locus_tag="HUW33_RS29655"
                     /old_locus_tag="HUW33_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2530..2874
                     /locus_tag="HUW33_RS29660"
                     /old_locus_tag="HUW33_29655"
     CDS             2530..2874
                     /locus_tag="HUW33_RS29660"
                     /old_locus_tag="HUW33_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3083..>3517
                     /locus_tag="HUW33_RS30100"
                     /pseudo=""
     CDS             3083..>3517
                     /locus_tag="HUW33_RS30100"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3552..4061
                     /locus_tag="HUW33_RS29670"
                     /old_locus_tag="HUW33_29665"
     CDS             3552..4061
                     /locus_tag="HUW33_RS29670"
                     /old_locus_tag="HUW33_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4068..4280
                     /locus_tag="HUW33_RS29675"
                     /old_locus_tag="HUW33_29670"
     CDS             4068..4280
                     /locus_tag="HUW33_RS29675"
                     /old_locus_tag="HUW33_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4947..5171
                     /locus_tag="HUW33_RS29680"
                     /old_locus_tag="HUW33_29675"
     CDS             4947..5171
                     /locus_tag="HUW33_RS29680"
                     /old_locus_tag="HUW33_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            5155..5421
                     /locus_tag="HUW33_RS29685"
                     /old_locus_tag="HUW33_29680"
     CDS             5155..5421
                     /locus_tag="HUW33_RS29685"
                     /old_locus_tag="HUW33_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          2199..2278
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1616..2013)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2865..4396
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attatggcct cagagttatc atcagaagca gctctctgca ttactacttc gattcccttt
       61 tctttgaatc tataagtatc cagttgccta ttaaacttag aaagacctac aaccatactt
      121 agtttgttat ctttaacagt aagagatgct tcctgagtac cattacatcg gtagtaagcg
      181 gtgtaaccgt cagcaaaaga tcgaccgggg aatataaatg ctaggaacgc aattaaccaa
      241 tacatagttg agaatcttgg tattacacat ttcactatcc tctctttcat ttttcgttca
      301 ccatactttg ttatctgatt tttaattttt ctattttata aaccaagtct ctaccaaaaa
      361 ggcgaaaatt atcctcaaat aataataatt gtttaagaac atccatgaaa gcagctgctt
      421 caccctcagt tatgggagct actctatata acatataacc tgagttaagg gtttctttta
      481 tactttcttc aatgctcatt ataaatatta gtggattatc atttttcttc ccccatgggt
      541 ttctaattaa tccttcgatg tactcaggtc ctttgcgtcc tattgcctgc gcaatatttt
      601 ttatttcatc tgcgtgaggg ctttgttgta cagctttgtt tatgcctgct tgagtgattt
      661 tcagagactc tacaccgtgt atgttgtatg agataaaatg taataaatta agatgcaatg
      721 caattaatcc atttttgttt acatctgagt atttggataa aaaacgtaat gaagtgttaa
      781 ctctcccttg aagcctaaca attgggagtg ctgccttgta tatttccttc tccgttaaat
      841 accgtttaaa aaacataatt ctccgcctcc gtatagcgtg cctattttat ttcgtcgtaa
      901 aacttagctc tgaaatattc ctttagaacc atcatgtcat taataattga atctatctca
      961 tccatttttt cttggtattc ttttaatggt gaagggagcg gtaactcaat atttgaaaag
     1021 tgattggcga gatagggatc aaggtttctc atccattcag gcttttcgcc aatgcgttct
     1081 tcaactgaaa gtgaacctga taatctctgg ttaataagat cacttagact cattttggtt
     1141 ttattcgaaa gccgttgtaa ggattgcttt aatcctatat ctagcatgat atttgttctt
     1201 tctctcatta cccccccttg ctattgcgtt ttttgttgcg cacaaagcta ccattgcgca
     1261 ttaagaaacg caataaaaaa ggagggaggg agtggtttgt acatgatgtg cagtgcgctg
     1321 agggcatcag tggcgggtgt ttttccgtgg gatggatgga gctccgggga taacagaact
     1381 gacgatcatt taaaggatct tcttgagatc ctttttttct gcgcgtaatc tcttgcactg
     1441 taaacgaaaa aaccgcctgg ggaggcggtt tgatcgaagg ttaagtcagt tggggaactg
     1501 cttaaccggg taactgggta tacgagaccg cagataccaa aactgttctt tcagtgtagc
     1561 cgcagttagg cacccacttc aagacctctc aatatctctc tcgtatatat ccagttacca
     1621 atggctgctg ccagtggcgt ttttgcgtgt ccgtccgggt tggactcaag atgatagtta
     1681 ccggaaaggg cgcagcagtc gggctgaacg gggggttcgt gcatacagcc cagcttggag
     1741 cgaacgacct aaaccgagcc gagataccag cagcgtgagc tatgagaaag cgccacgctt
     1801 cccgaaaggg aaaaaggcgg aacaggtatc cggtaaacgg cagggtcgga acaggagagc
     1861 gcacgaggga gccgccggag agaaatcacc ggcatcttta agtcctgtcg ggtttcgtcc
     1921 tcctctgact tgagcgtcca tttctgtgat gctcgtcagg ggaggcggag cctatggaaa
     1981 aacggcaacg ccgcatcctt ttctccggcc ctttctgatt tgagcgcctt tgctttatcc
     2041 cgttatccgt gagtcggtca cgcccgccgc agccgaacgg caggagcgca gctaccgagt
     2101 gagcgaggag gcgtcatttc atctgtccgt gcatggtatt gcggacgctc ctgagccaca
     2161 tatcacacct gccacatgag gcactttcat gaatcgtgct ctcatgccaa catagtaagc
     2221 cagtatacac agcgtaaacg ctgtgactgg ttcagggctg cgccccgaaa cccgctaaca
     2281 ccactgacgc gctggcgctt gtccaacgcc ggagcgaccc ggaaaacttt tccgtgccgt
     2341 ccggcgtttg cgaaggaggg ataatgcaga agccgaagaa gaagccgctc gtcgtaaaaa
     2401 aaagatcctg gcatccggca gtgatggcaa atctgatgcg tcaggcacag gaggaagatt
     2461 accgccggga gcagatggca gagcttgagc gttcagcccg gtcacatttt gcggatatcg
     2521 gaggagagga tggcggttaa gcgtacccgt tttttaggta tccgggtgac cgatggggag
     2581 taccagcagc tgctggagcg ctgcaatgga aggcaactgg cggtgtggat gcgggaaacc
     2641 tgtctcgata cgcgcccggc gcggtcgttg cggctgccgt ccatcgatcc ggttttgctg
     2701 cgccagcttg ccgggatggg gaacaacctc aaccagatcg cccggaaaat taacggcggc
     2761 cagtggtcag gcgctgaccg ggttcaggtg gtggccgcgc tgatggccat tgatgccgaa
     2821 ctcgagcggc tgcggcatgc cgtgctggaa aagggggcag acgatgatcg ttaagtttca
     2881 tccgcgaggg cgaggcggcg gtgccgggcc ggtggattat ctgctgggga aagaccgcca
     2941 gcgcgacggg gccatcgtcc tgcagggaaa gccggaggag gtccgggagc ttatcgacgc
     3001 ctcgccctac gccaaaaagt acacctccgg cgtcctgtct tttgctgaac aggatttacc
     3061 gcccggccag cgtgaaaagc tgatggcgag cttcgagcgg gttctgatgc ccggactcga
     3121 taaagaccag tacagcgtgc tgtgggttga acaccgggac aaggggcggc tggaactgaa
     3181 ttttctggta ccgaacacgg aactgctgac cggcaggcgt ctccagccgt actacgaccg
     3241 ggcagaccgt ccgcgcatcg atgcgtggca gaccgtggtg aacgggcgac tggggctgca
     3301 cgacccgaac gcaccggaga accggcgcgc gctggtgacg ccatcttcgt tacccaaaac
     3361 gaagcaggaa gccgcagagg ctattacgcg gggcttactg gccctcgcct cgtcagggga
     3421 gcttaaaacg cgtcaggacg tcactgaggc gctggaaagc gcaggttttg aggtcgtgcg
     3481 caccacaaag agcagcatca gcattgccga cccggacgga gggcgaaaca tccgacttaa
     3541 gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag aaatcgaaag
     3601 cgcagcagca gagtaccggc gaaatgctga aagccgcatt cagcgagcac gagaagtctg
     3661 tcagagcgga actgagcgaa agcgggaaga gaatcagcgc cgccatccgc gcccacgagc
     3721 agggtatgag cgcggccatg cagtcgaacc gcctgagcgt gatgcgtatg gtcgggcgga
     3781 catggctgac catcacgatg gtgtcaggac tgctgttcgc cagcctgagc ggggtgttgt
     3841 ggtatcaggg gagcttgata gcgtcaaatc tggccgaaat cgaccggcag aacgcagcgc
     3901 tgtcgaagct gaacgcgaag acctggggcg tgacgtacct ggaagacagc aacggacgtt
     3961 ttctggtgct gccgaagggg acggccgtag acaggacgca gagctggacg gtcgggaacg
     4021 ggcggtcgaa gcagaacgcg ctgagactgg tgaaggagta agtgccgatg accgagctgg
     4081 aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa aggctggacg
     4141 agtgggagag cgccttcggg gaatggcgga aaatgtcagg gcttatgcaa cgggagaacg
     4201 cggcgctgag cgagcgcgtg acgcgcttga gtcagcaggt ggaacgcttg agcgggcaac
     4261 tgcagcgttt gagccggtag tgcagcgtca tgagatggcc gtggccgcag aacgggcgca
     4321 cgagcaacgg cagcacgaaa aggaactgac ggaagcgcgt tcccggcagc gaagctatga
     4381 cggtccgtcg ctgggctgaa atgcaggaaa acagggttct catcgggcat ttacgctgaa
     4441 accggatgtc attcacctgg ctgcatggct atgcagccag gtaaaattta tctgtcgcga
     4501 acggggcggt ttatcgggtg gtttgttgcc ggttttacct gactgccacc ccgttcgcgg
     4561 cgaacccgtc cggggcggtg cgggcaacgg gtgttatgtt taatcagcgt gaacggcaga
     4621 tacaaaaaag ccccggaggg cttttaacta ctgacttatt tttttgaaga tttgctgggt
     4681 ttcaatttgt cagtatttcc cggcgttttt ttattgtcac gctggcgttt atcaggttcc
     4741 cctgttgaat cggcaattcg tttcggaccc ggcttaagac ccataatcgt tatcctttca
     4801 atagttaaga ggaaagccct caagctagat atattgaccg cgttacggat tatcaagagg
     4861 ggctaacaag attatttttt gttcttttag gttgattgtg gtactgttga tatacatgta
     4921 tagcaaatgg tgttcgggag gtttatatgc ttgctatccg gttatctgat gagattgagt
     4981 cccgtctgga ctcgctggcg aagcaaaccg gcagaacaaa gacgttttat gcgcgggaag
     5041 caatactggc gcatctggaa gacctggagg attattatct ttcagcagaa actgctgcac
     5101 gcgttcgccg tggtgatgaa gcagtgcatt cgtctgaaga cgtgaggaag tcacttggtc
     5161 tggacgatta actattccga tcgggcgctc aaatcgttac gcaagatgga caaacagaac
     5221 gcacgacgga ttgtggattt tatgagttta cgcattgcag ttgctgccga tcctcgccag
     5281 tcagggaagc cgctcaaagg tgagctgggc gagttctggc gctatcgggt gggagattat
     5341 cgcgttctgt gtgagatccg agatgacgag cttgttatcc ttgccgccac gattggacat
     5401 cgccgcgaag tttacgactg aagcccgcta tgcgggcttt tttgatgccg acaacaggta
     5461 ttatggtaaa tactcgacta actacgtgtt ctactgtttg tcggttttag ttaaccagca
     5521 ggttgtctta acgataatgt agccgttatt tttaactaat aaggtcatat tgttaacgtc
     5581 aggcataata aaagac
//
