LOCUS       NZ_CP055299            11970 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP55 plasmid pKP55_5, complete
            sequence.
ACCESSION   NZ_CP055299
VERSION     NZ_CP055299.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15234688
            Assembly: GCF_018336835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Li,X.
  TITLE     Ceftazidime-avibactam resistance in carbapenem-resistant Klebsiella
            pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Quan,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2020) Infectious Diseases, Sir Run Run Shaw
            Hospital, 3# East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-JUN-2020
            Assembly Method                   :: Unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 11:30:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,982
            CDSs (total)                      :: 5,860
            Genes (coding)                    :: 5,681
            CDSs (with protein)               :: 5,681
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 68 of 179
            Pseudo Genes (incomplete)         :: 118 of 179
            Pseudo Genes (internal stop)      :: 25 of 179
            Pseudo Genes (multiple problems)  :: 32 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055299.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP55"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP55_5"
                     /geo_loc_name="China: Hangzhou"
                     /lat_lon="30.3 N 120.2 E"
                     /collection_date="2020-04"
                     /collected_by="Xi Li"
     gene            join(10564..11970,1..279)
                     /locus_tag="HUW33_RS29570"
                     /old_locus_tag="HUW33_29565"
     CDS             join(10564..11970,1..279)
                     /locus_tag="HUW33_RS29570"
                     /old_locus_tag="HUW33_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            289..546
                     /locus_tag="HUW33_RS29575"
                     /old_locus_tag="HUW33_29570"
     CDS             289..546
                     /locus_tag="HUW33_RS29575"
                     /old_locus_tag="HUW33_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            631..780
                     /locus_tag="HUW33_RS29580"
                     /old_locus_tag="HUW33_29575"
     CDS             631..780
                     /locus_tag="HUW33_RS29580"
                     /old_locus_tag="HUW33_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2264..4228
                     /locus_tag="HUW33_RS29585"
                     /old_locus_tag="HUW33_29580"
     CDS             2264..4228
                     /locus_tag="HUW33_RS29585"
                     /old_locus_tag="HUW33_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4228..4959
                     /gene="mobC"
                     /locus_tag="HUW33_RS29590"
                     /old_locus_tag="HUW33_29585"
     CDS             4228..4959
                     /gene="mobC"
                     /locus_tag="HUW33_RS29590"
                     /old_locus_tag="HUW33_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4966..5496
                     /locus_tag="HUW33_RS29595"
                     /old_locus_tag="HUW33_29590"
     CDS             4966..5496
                     /locus_tag="HUW33_RS29595"
                     /old_locus_tag="HUW33_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5523..5702)
                     /locus_tag="HUW33_RS29600"
                     /old_locus_tag="HUW33_29595"
     CDS             complement(5523..5702)
                     /locus_tag="HUW33_RS29600"
                     /old_locus_tag="HUW33_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5728..6156)
                     /locus_tag="HUW33_RS29605"
                     /old_locus_tag="HUW33_29600"
     CDS             complement(5728..6156)
                     /locus_tag="HUW33_RS29605"
                     /old_locus_tag="HUW33_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6320..6634)
                     /locus_tag="HUW33_RS29610"
                     /old_locus_tag="HUW33_29605"
     CDS             complement(6320..6634)
                     /locus_tag="HUW33_RS29610"
                     /old_locus_tag="HUW33_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6622..6900)
                     /locus_tag="HUW33_RS29615"
                     /old_locus_tag="HUW33_29610"
     CDS             complement(6622..6900)
                     /locus_tag="HUW33_RS29615"
                     /old_locus_tag="HUW33_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7075..7440)
                     /locus_tag="HUW33_RS30015"
     CDS             complement(7075..7440)
                     /locus_tag="HUW33_RS30015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7437..7808)
                     /gene="tolA"
                     /locus_tag="HUW33_RS29625"
                     /old_locus_tag="HUW33_29620"
     CDS             complement(7437..7808)
                     /gene="tolA"
                     /locus_tag="HUW33_RS29625"
                     /old_locus_tag="HUW33_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8082..8327)
                     /locus_tag="HUW33_RS29630"
                     /old_locus_tag="HUW33_29625"
     CDS             complement(8082..8327)
                     /locus_tag="HUW33_RS29630"
                     /old_locus_tag="HUW33_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8972..10459
                     /gene="ltrA"
                     /locus_tag="HUW33_RS29635"
                     /old_locus_tag="HUW33_29630"
     CDS             8972..10459
                     /gene="ltrA"
                     /locus_tag="HUW33_RS29635"
                     /old_locus_tag="HUW33_29630"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             5564..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(884..1462)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4229..4956
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tacggccatg attatcatcc agtacctaaa acagaagaca taaagggact tggagagtta
       61 aagcgaggtg atcctaaaac acctaaacaa ggtggtggtg gtaagcgggc ccgatggtat
      121 ggcgataaaa aacgtaaaat ttatgaatgg gattcccagc acggtgagct tgaagggtat
      181 cgtgccagtg atggcgaaca cctaggcgca tttgatccca aaacgggtaa gcaggttaaa
      241 gggccagatc caaaacggaa cattaaaaaa tatctttgag aggtaaatat ggggcttaaa
      301 ttacgtttga catggtttga taagaaaact gaagagttta aaggggaaga gtattctaaa
      361 gactttggtg acgatggttc tgtcattgaa agcttgggaa tgcctttaaa ggataatatt
      421 aataatggtg agtttgattt gaagaaagaa tgggttcctt tactacagcc attttttaaa
      481 aatcaaataa aacctgatgt ggataattgt tatatagctt ttgattatcg ggacggtaac
      541 tggtaacaaa aaattatcaa ggatgatttg aaatactgac ttccggtgtt cagggatgaa
      601 tgccggagtc tttaaataca gggaaagtat atgaaaaagg taaagactat ttttcttttt
      661 atattgattg ttgctggctt tcttctggcc gcatgtcagg caaattatat ccgggatgtt
      721 cagggcggaa cggtggcacc atcgtcctcc tctgaactga cggggatcgc ggttcagtag
      781 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa
      841 acgaaaaaac cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt
      901 aaccgtggta actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg
      961 gactttggcg cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact
     1021 cccagttacc aatggctgct gccagtggcg ttttgcgtgc tttcccgggt tggactcaag
     1081 tgaacagtta ccggataagg cgcagcagtc gggctgaacg gggagttctt gcttacagcc
     1141 cagcttggag cgaacgacct acaccgagcc gagataccag tgtgtgagct atgagaaagc
     1201 gccacacttc ccgcaaggga gaacggcgga caggtatccg gtaaacggca gggtcggaac
     1261 aggagagcgc aagagggagc gacctaccgg aaacggtggg gatctttaag tcctgtcggg
     1321 tttcgcccgt actgtcagat tcatggttga gcctcacggc tcccacagat gcaccgaaaa
     1381 agcgtctgtt tatgtgaact ctggcaggag ggcggagcct atggaaaaac gccaccggcg
     1441 cggccctgcc gttttgcctc acatgttagt cccctgctta tccacggaat ctgtgggtaa
     1501 ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc gaccgagtga
     1561 gcgaggaggc gcaatttccc cggcacctgc aagacggtca ccggacgcgc tgatgtggga
     1621 tattgtcccc tgattaagtc tttctattcc ctgacgatca attatcagga taggtgaacc
     1681 atgtcggttt ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttgc
     1741 ggagaaatgc aagtcatccc cgctggggcg tggctgactt caggggaaaa gaccttgctt
     1801 acgctggctg gacgccaccg acgcacggtc ttttcccctc tttctggctc tgtccggctg
     1861 gacgccggcc acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca
     1921 aaaatccacg gtgctggacg cacctgacga agcagcaaag ctgctcctga ttttgcgggt
     1981 ggtcagccgc tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact
     2041 ggggtgccgc cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc
     2101 cttttccgca tggggcggaa agaggctggc ctccgcggtg aaagctgaac gcaatcacca
     2161 cggacgtgac cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg
     2221 cgcatcacgc cggtaaaccc gtcgtctgaa ggagagagat tgcatgttta atacggattc
     2281 gcttgcctgg cagtggcata tcccggagct ggcccggcag atttacccgt gggtcagctg
     2341 gctgtaccgg gagtattaca gtctggccgg tttttttctg gttatcggcc tgtttttcgg
     2401 tctggctgaa ctctgggccg gccgaatgcg ttttgtgggg ctgagccata aggcgcggcg
     2461 ggagtatgcc gatcgctata agcgcgataa taaccggact gtgccatttt cggtgctggc
     2521 ctcgctggat tcacccgggc ggatggtcag ggcacggcga catgtctgga actacgcgta
     2581 ctggctgacc ggcgctgccg gtctggccct tctggggggg ctggttgtcg ccgtgtttat
     2641 cgagagctgg ggagaaaccg tcctgcgccc ggacctctat gcgaacgcag attattacct
     2701 gctgcagccg gctaacctgt tcgggatagc ctggtgtgcc ggagggcttg tcgccggcgt
     2761 ccttctggcg cggctgctgg cgttcctgct gcttgatggt cattttgcgg ccgctgacga
     2821 ggcggtgaac gcgaaactga atcaggagag ccggcgcagc agccagcgga ccggagaaat
     2881 gacagacgtc cgccacctgc atttcgggga gccggtaccg gttaacgcgc tggccgattt
     2941 ttccacggag caggcccgga aacagcaggc ggtgtttctg ggtaaggatg agcagggcca
     3001 gccggtgctg gtccctcgtg atacctggcg caagaccaat attcagatcc tcggcctgcc
     3061 cgggagcggc aagagcgcga tggccaccaa cgcgctcatt cgctgcgtac gcgatttcgg
     3121 ggacgctgtg gtgtacttcg accccaaagg ggacgcgtgg gcgccgcacg tcttccgggc
     3181 gcactgcccg gacttcaccc tgctggatct gcggccgggg aaaccggcgc agctgaatct
     3241 gttccgcgat ctcgaccagt acgccctgaa gaatctgctg gttgccgggt ttaacctgag
     3301 tgagaccggc gacgtggcgg accactaccg catcagcgag cagaaagcgg cgaagctgat
     3361 tgcggagcag ttcccgcagg gggcaaacat tcagcaggtg ctggcggcgg cgtacgcgtt
     3421 gccggaggcc ctgaaaaagg acgtgaaggg gctgatcacc aagctggaga acgtggccga
     3481 cctgagcgtc ctgcagacgg acagcggtat cgatgtggcg gggataatca acggcggcgg
     3541 ctgcctgtac gtcatcggct cgatggacga tgaggcggtg atccgggtgc agaagatgct
     3601 gtttgcccgc tgcgcgcaaa tcatcattgc gcgggatgag ttccggaggt ggccgcacgc
     3661 gagcatcatg ctcgatgaga ttaaatatct gctgtcgaag tacgtgctga acgcgctggg
     3721 gacgctgcgc agccgggact gcaacctgct gctcgcgcac cagtcgctgg gcgacttcgg
     3781 tcagtgcggg caggatctgc ccgctgattt tgtgaaaacc acggtactgg ataacacgcc
     3841 gatccgctgg ttctatcgcg cggccagcca ggagtccgcg cagtgggcgg cgggccagac
     3901 cggggagatc cgggtggacg tggagcgccg ccgggccagc cgggaggcgg ggaacgtgga
     3961 gcacatcagc ggcgacagct tcatccagaa ggaggcgcgg ccgctgtttg acgtgaacac
     4021 cctgcaacac ctgcctgacg ggttcgcggt gatgaccggg ctgggcgtgg cgcggctggc
     4081 gttcagcagc ccgctgcggg ttgagcgccg ggaaatttcg ctgaaaacct tcccggtgct
     4141 ggcgaaaacg gacccgctgg cggagtacct gcctccggcc gacggcggcg gaccaaaagc
     4201 ggatgatgat tttgcggggc tttactgatg acactggagc agtacaacgt cagccacgcg
     4261 aagcggcagg cgcggaacgc ggagaaaaca cggctgaccc tgcgctggct gcgggaagag
     4321 ctgtgttcca cggcggagct ggttgcgcga cggctgggga ttgcggcggt acagccggtt
     4381 taccgcttcc tggacagtct ggtggcgaaa gggctgctgg tccgggcgaa atacccggtt
     4441 gacggccgtc aggtcagcgt gtgggggctg acgccgcacg gggtggcgtt ctcgttcgac
     4501 gaggacgagc cgctgacgga cgttattccg tttcagccgt caagggtgtc ggcggcgcag
     4561 ctgccgcacc ggctggccgt gcagtccctg cgcctctcca tggagggccg gggggccacc
     4621 ggctggcggt acctgcaccg tatggccctg aaagggatga aagtgccgga cgcgctggcg
     4681 gagctggacg gcagaacggt ggcatttgag gtagagcgca ccgtcaagag ccgcaggcgg
     4741 tatcaggaag tggtgtccgg gtatctgttc aaccgccggg cgaacggcat cgatgaaatc
     4801 tggtacatct gccctgaccg gccgacgctt gtccgggtac agcgggcgat tctggcggtg
     4861 gatgaaatcg tgaatccaca gaccggcgag gcccgcaaaa cggtggagct ggaccgggag
     4921 aggctgttcg cgtgctttaa atttatgaca acggagtgag ggattatggc gctgtacatg
     4981 gtatttattg aggacgtgga agacaaagac ggcgagcgct gcattatgcc aaccagcgag
     5041 catctggaag acatggtggt gctcgcctgg cagaccgggg cgtatgtttt ctgttttgtc
     5101 gaccgggccc agcatctggc gatgatgaaa tccatcggga tgtatgacga gcggctcaac
     5161 agcgacgaaa aagccacgta ctttttcacc ggtgaaaatc cgcaatggga acgggacaaa
     5221 cccacaggaa tgaagctgtg tgattacctc tatcgaaagg tggagcggtt cgccatcgag
     5281 aacggtgtgc aggtggttga tgccgggaag tgctcaccgg agcagattaa cgcgatgatt
     5341 gaggtggtca gcgagcggca cggggaaagc gcggtgaacg agaagctgaa agcggagctg
     5401 atgcgggaat ataacagccg ctattttaac gtgccggtgc ctgaactgcc ggaggacaaa
     5461 cccgcccgcc ggtggtggcc gttcggcaaa aaataacgcc agcaccggca tggccggtgc
     5521 tgttattctt ccccgagctt atcccgcatc acctgccaga gtgtttccgc atcctcgtga
     5581 agccgttcgc acatgtcggc cggggtatcc atgtccagct cgttcagctt ttccagcaac
     5641 agcagcgact gatccttaat gaatttagcc atattgaggg cagttttttc ctgcttcgtc
     5701 atgttctcca ttcccttcgg taagttctca gctaaaccag ccggaccaga agcgtttttt
     5761 cttctgtgtc agttctgttg tcaatctgtc cacttcggcc tggagtttcc ggcgctcatc
     5821 atcgtgctgg cggcgttctt cttcccggga tgttttatct tccagcatca gggtgacagc
     5881 ctgccggaga tcggccagtt ccttttgcat tgccagaagc tgttcctgtg ggaaattttt
     5941 ttgtgtattt tcacggtgtg aaatgccggg aatttgtttc tcagggatat tcacatttcc
     6001 gtatacacgg atcagttctg acacatccac gacagggttg tttttcccgt cacgtgacac
     6061 ggtaaccttt ccctgtttta catgattgta tagcgttctt ctggtaatgc ccgcggcttg
     6121 tgctgcctgt gaaaggttga gcaatgtttt cgccactgta tacccctgat tttatcgggt
     6181 gtgaaaggtg tgtaatttct cacggtaaat gtatcgctga attttacccg tttttccgtt
     6241 caggatgtgg ttttctgacg cgctgcgctt gtcaaaccgg cgacggccac gcaatgaatt
     6301 gcgcccccgt catcggttat tagaggctgt ctgcccccgg atacccggat tctttctgat
     6361 gcctgacggt gacgatcaca atctggtcca tttcccggtc gtggcggtag agcatcacat
     6421 agccactgtc gccaaacccg atcaccagtt ccttatattc cagcggcaga aacggcaccg
     6481 ggcggccaat gtctggcaat gttttcagtt gctggatagc ccggacgatc acctcacctc
     6541 cttttctggc tgccagccga tttttggttt tgagaaagtc ctgaaggcgt tgtaaatcgc
     6601 gctgtgccag cgcggaaatc gttacctgtg gcatggcgga gcatcctgct cgttctcagt
     6661 tccccaggta cttatccagg cttccgcttc ttccgcagta aggtgcagac ccgtttcctg
     6721 atagtgctgc catgcggcct gtccgtcccg caggtactgg ctgcgcttct cttcccggtc
     6781 gatgtattcg gtgattgcct ccagcattag cgcgtgcgct gagcggtgcc ggtcatcggc
     6841 cagcgttttc agccggtcct taagttcttc atcgagcctg atggatgttg ctgatgacat
     6901 aaaagcccct ttgtagtcat atgtattaca catgactact ttaatccgtg gcggcgctat
     6961 ttgccagaaa tacggctttt gctgcccatt tttcagcttc gcttccacta atcagggctg
     7021 ctacaggctc actcatcatc caggactatt gcagcctgcg ggttaaagga cctgttactg
     7081 gcggcaacca tcatcattga gcttttgggt ggtgactggt cgcggatcct gtaagccgtc
     7141 atttttcaga ctgaattcaa aatttataac ctgtcgcatt gccgggtgcc ctgcgggata
     7201 tagccagtgc ctcattgccg tcagcgtttc ccgaaaaaaa aggtgacagc cggtaagcga
     7261 cacgttgtcc actcgtccat cgtccagaat atcaaacgtg gcggtcacct gaccttcgat
     7321 gcgttttgct gcggcctgta ccgggtaggt tggctgcagt gtggttacgg gttttagtgg
     7381 tatcgcatgt accggagtga ggaacatggc cgccagcact gtcagagtaa aagctttcat
     7441 ggtctgaaat ccagaggggc gtttttgaat atttgccagg tattttcatc cggtgccggc
     7501 ggaattttgg cgtgggtaac ggctgatatt gctgctttac agagtttcgg atctcctcct
     7561 tcagcaatgg cgctgttcag agtgccatca cgttgtatgg atatccgcac ggtgcacatt
     7621 tttcccgcat aggcctctgg ttcaaacatc tgcatttcaa cagcggtctt gatggccgca
     7681 gcgtagctac tgattttaga tgacttatcc agagtataaa ttttcggcgg ctgggtggtg
     7741 gaatgcttac aggccgtcag gagtaagggg ctgcacaatg ccagcaggat aaagggtatt
     7801 aaacgcatcg ggttgatgac ctgtcagtaa aaaatgttac atgtatattt gtcactcttt
     7861 acggccacgt tcatttacat aaacacggct tatcgtatcg tttttacacc aaacggcccg
     7921 ccatgcagtt gcgtgcatag gcatgcagcg ggttacaatt taccgggcgc gtttccggcg
     7981 gttttccggg tggtttgttg cctgttttat cccgtagccg ccggaaacgc cctcagaccg
     8041 tctgagcggt gcgggcaatg cgacgttatg gtaaataccc cttaccggcg gcgctgtggc
     8101 tgccaggtga aggtggtttc cgtagcctcg tatttctccc gacgcatttc ctgcgcccgc
     8161 ctggcttcac gttcgtcctg aatggcacga agcacccttt cccgcacctg ttgttttacc
     8221 gcctttggca gcgttttgtt ccctttcagg cccatggcca gtgcctgagc gacaaaggcg
     8281 tccgccaggc gcttttcttc agcgggagtc agtttcaggt gcgtcatagt ggcctccggg
     8341 cgtctcaggt ttcccgaaca gtttaaccgc aggtcctctc ctcatgccag ttagctttca
     8401 gatgtgtcta taccatctga aatgtaagtg tgatgaatgt cttaaataat aaaatttgtt
     8461 gccatgagaa attttcggga aaagacgcga tctgcttaca ggtggctccg ggcgcttttt
     8521 tgtgcctgaa gatgagaact aatctcatat attgacacct gaaaacggga ggagtaaggt
     8581 aataattata ctgtgtatat gtacagtgtg cgccgggaga ccggttagag atcaaggggt
     8641 gaaagtcccc gaccattgaa ggaccagcaa tccacaaggt ccccgagtca tgcgttgcat
     8701 accgcgaggt atggggcgaa gcgttgacag gggtgttgac aggccagcca ttgagccacg
     8761 aaatgtatat taaattaccg ggtgccgacg ttgtactgtt aacggaaggc aacatcatag
     8821 ggtgcgatac tgcgagtgcc acatggaccc ggcggggtct gagaccctgg catgtcaata
     8881 cgatctctac gcgggaaccg ggagatctcc cctctgacca tctgccagtg tcggagatgg
     8941 cccgcaccgg gaagacgagg agtcatagcc ggtgatgtac ggagaggaga agtcggactc
     9001 gctcatagta gcggcgaagc aggcgaacaa cccgaaagga gcggagtcag tggagcgaag
     9061 gagcggggcc aaggggaacg cggaacagcc acacatgcgc cggacacaga gccgggaaag
     9121 catgtcacag aggctgtcac gcgtgcggga agctgcgaag cagcggaaga aagaacggtt
     9181 tacagcattg ttccacctgc tgacagtcga agcactggaa gccgcattcc tctccctgag
     9241 caggaaagcg gccgccggag tggatggcat caggtggatg gactacgccg gaaacatgaa
     9301 gaacaacata acagatctgc accggaggct acatcagggc agctacaggg cgcagcccgg
     9361 caggcgtcac tacatcccaa aagcggatgg aaaacaacgc ccgctcggca tcgcctcgct
     9421 ggaggacaag atcgtccagt atgcgctggt gaaaatcctg aacgcagtct atgaaaacga
     9481 ctttatgggg ttctcatacg ggttcagacc cgggcgaagc cagcacgatg cactggacgc
     9541 actggccaca gggctggtac gcactaacgt aaactgggta ctggatgccg acatcagtca
     9601 gttcttcgac agggtgagcc acgaatggct gatcaggttc acagagcatc ggatcggcga
     9661 ccggagggta atcaggctca tacgtaagtg gctcacagcc gggacgtcgg aggagggtca
     9721 atggcgagca acggaggaag gcaccccaca gggtgcggtc atctcaccgc tgctggcaaa
     9781 catatacctc cactacgtct tcgatctgtg ggcgcatcag tggcgacgtc gctatgccac
     9841 aggcaatgtg gtaatggtca gatacgccga tgacatcgtc atcgggttcg acaaacgata
     9901 cgatgcccgg cgcttccgta tagccatgca gcgcagactg agggagttcg gactcacggt
     9961 tcacccggag aaaacccgtc tgatggagtt cggccgcttc gctgccgaaa accgtgccat
    10021 caggggaaaa ggcaaaccag aaacgttcaa cttcctcggg ttcacgcaca tcagcgggaa
    10081 agatcgcaac ggcaggttca tgctgatacg aaagacccgc cgggatcgga tgacggcaac
    10141 tctgaaagcc atcaaagacg gtctgcgaag gcgctggcat tactcaatcc ccgaacaggg
    10201 aaaatggctc aggagagtgg ttcagggata cctgaactat cactcggtac cgggcaactt
    10261 ccccaccatg cagaagttca ggacacacgt aacaaacctc tggcgccggg cgctcaggcg
    10321 caggagccag aaggatgata cgacctggac gaaagcaaac aaactggcag ccgcatggct
    10381 accaagggtt cgggttcttc atccatggcc tgtggagcgg ttcaccgcca gacacccgag
    10441 gcaggagccc ggtgcgtaaa tcgcgcacgc cgggatctgt gcggggggta tccggtaacg
    10501 ggtatcccta ccgcgacatg ttggctttaa tttattgttt taaatgtcaa agaggaaaac
    10561 ggtatgagtg gtggagacgg tcgaggtccg ggtaattcag gtctgggaca taatggtggt
    10621 caggccagtg ggaatgtgaa cggtacgtct ggtaaaggtg gcccttcatc aggtggtggt
    10681 acggatccaa acagcgggcc gggctggggt acgacgcata cgccgaatgg agatattcat
    10741 aactataatc cgggggagtt tggtaacggc gggagtaaac ccggtggtaa tggtggtaac
    10801 agcggcaatc atagcggtag ctctggtggc ggacagtctt cggccactgc gatggccttc
    10861 ggtctgcctg ccctggctac tccgggggct gaaggactgg ctttatcagt ttccggcgat
    10921 gcgttgtcgg ccgccgttgc tgatgtgctg gctgccctga aggggccgtt taagtttggt
    10981 ctgtggggga ttgcgatcta cggtgtgctg ccttctgaga ttgcaaaaga tgatccgaaa
    11041 atgatgtcaa aaattatgac gtcattaccg gccgatgcgg tgacggagac tccggcaagt
    11101 actttaccac tggaccaggc gacggttcgt gtcagacaac gggttgtgga tgtggtgaag
    11161 gatgagcggc agcatattgc ggttgtcgca ggtcggccaa tgagtgtccc tgtggtggat
    11221 gcgaaaccga caaaacgtcc gggggtattc agtgtgtcga ttccgggtct cccgtctctg
    11281 caggtgagcg taccgaaagg tgttccgaca gcgaaagccc cgccaaaagg cattgttgct
    11341 gaaaaaggtg attcacgtcc ggctggtttt acagccggtg gtaactcccg tgaggccgtt
    11401 attcgtttcc cgaaagagac cggacagaag ccggtttatg tgtcggtgac agatgttctt
    11461 accccggcac aggtaaaaca gcgtcaggag gaagaaaagc gtcgccagca ggcatgggac
    11521 gccgctcatc cggaagaggg gctgaaaaga gactatgata aagcgaaagc tgagctggat
    11581 gccgaagata aaaatattgc gaccttaaac agccgcattg catcgacaga gaaggcgctc
    11641 cccggtgcaa gggctgctgt tcaggaagcc gataaaaagg taaaagaggc agaggcaaat
    11701 aaagatgatt ttgtgactta taacccgcct catgaatatg gctccgggtg gcaggatcag
    11761 gttcgctatc ttgataagga tattcagaat cagaatgaga aattaaaagc ggctcaggca
    11821 tctttaaacg caatgaatga atccttatcc agagataagg ctgcacttac cggggcgatg
    11881 gagagccgga aacaaaagga gaaaaaagcg aaggatgcag aaaataagtt aaatgaggaa
    11941 aagaacaagc ctcgcaaggg gactaaagac
//
