LOCUS       NZ_CP055291             8955 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH9, complete sequence.
ACCESSION   NZ_CP055291
VERSION     NZ_CP055291.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055291.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH9"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            join(8717..8955,1..142)
                     /locus_tag="HUK62_RS18820"
                     /old_locus_tag="HUK62_18820"
     CDS             join(8717..8955,1..142)
                     /locus_tag="HUK62_RS18820"
                     /old_locus_tag="HUK62_18820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086179912.1"
                     /translation="MSNDNRVTIRLSKEQYFLYEMRAAAKGMRVSTYVKSLLDECESSS
                     SNQLAELLRLFKSLDEKTSLITEVLMAQKDEKPAHTESGAVDNLSLEMLLMLRAMAKPE
                     QLKMAHARMREKDFPILNLQDY"
                     /name="hypothetical protein"
     gene            191..772
                     /locus_tag="HUK62_RS18825"
                     /old_locus_tag="HUK62_18825"
     CDS             191..772
                     /locus_tag="HUK62_RS18825"
                     /old_locus_tag="HUK62_18825"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_086179913.1"
                     /translation="MAIFLVRHAESEANINGKTLSHASIALSEHGHRQAQDLCSKLPVI
                     DHVIVSKYLRTQQTAAPILEKYGLSLEVDEHLHEFSYLSERKCANTNLNDRKAWVNAYW
                     EKMDYQHRDADDAESFENLYLRVQAFQEKLKILTPHYVKKNLAVFSHGQFLQLLIMQIQ
                     QPRSLTKELMQQFRSDLVRQPIRNTEVFTY"
                     /name="histidine phosphatase family protein"
     gene            complement(849..1721)
                     /locus_tag="HUK62_RS18830"
                     /old_locus_tag="HUK62_18830"
     CDS             complement(849..1721)
                     /locus_tag="HUK62_RS18830"
                     /old_locus_tag="HUK62_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="WP_176538638.1"
                     /translation="MSKIVEIFGLKTTHEPSDLIKAIADQTCPYSKKKCYKTRKSDPSI
                     AIGTCTLQYSSPIDKKAEKVSTIICPNRLLERKQIFIDCLHLLTGHTPGNELHLVPEVK
                     IPGGNVDFFLISAKDGKVKDFVGIELQTLDTTGTIWPERQNLLKELKIIDANTEVEKKP
                     FGMNWKMTAKTILVQMHHKVETFEHINKKMVLVIQDVLLDYMKKEFSFDHLNENVDIAD
                     SFHLHAYQLKNDNSKQDFMISLSKRLSTDADGIGVCLGLKTEAKVELEALIALLQSKIS
                     EHTSLTLEL"
                     /name="NotI family restriction endonuclease"
     gene            complement(1714..2814)
                     /locus_tag="HUK62_RS18835"
                     /old_locus_tag="HUK62_18835"
     CDS             complement(1714..2814)
                     /locus_tag="HUK62_RS18835"
                     /old_locus_tag="HUK62_18835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849183.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_176538639.1"
                     /translation="MNFPQNPDLFFQEIPIIGDNFGPKVEEYKKNDDTNPLFLLGDCLD
                     VLKQIPDESVDTIITSPPYWSQREYENGGIGLEKTLDDFFNNLLQITSELKRVLKPTGS
                     FWLNLGDVYKNKHLLGMPWRFAFKLIDEQNWVLRNEVIWNKVKGGMDNSKDKLGNVHET
                     IFHFVKDPANYFYSTDAIRSKPRQSKVVNGSIVSATGVSGVKYKRKIELSTELSEEEKN
                     NAFKALEEMLKSIQDGKYSDFRMVIRGANRTTHSNSDKVSGRAKELLTKGFYFLKYHPD
                     GSKPNDVWEILPESFSKNKEHFAPYPIDLCRIPILATCPNDGIVLDPFVGTGTTCIAAK
                     ILNRKSIGIDISKSYLDTAIRRVINE"
                     /name="site-specific DNA-methyltransferase"
     gene            2920..3276
                     /locus_tag="HUK62_RS18840"
                     /old_locus_tag="HUK62_18840"
     CDS             2920..3276
                     /locus_tag="HUK62_RS18840"
                     /old_locus_tag="HUK62_18840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538640.1"
                     /translation="MITEEFNTVRAFLEKMLEQNPDHKGFLDAYVKLIEAKSKFDLETN
                     KAIIEKEIRHSELNYDLLKTQDTNNANVHMNQNTNWADVNKTFNSNYHQTQQGYHNQAF
                     GLMNNALTNRDLLR"
                     /name="hypothetical protein"
     gene            complement(3371..3682)
                     /locus_tag="HUK62_RS18845"
                     /old_locus_tag="HUK62_18845"
     CDS             complement(3371..3682)
                     /locus_tag="HUK62_RS18845"
                     /old_locus_tag="HUK62_18845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538641.1"
                     /translation="MKFALLDHELNFLSLLDKLIDETDVFENKVLLIDKKLDFINKLIE
                     LEKAHLENNLKMEQADNDYRLERRKQGDNSELESKKQDDEYRLKRRQQDDDYSLNIRK"
                     /name="hypothetical protein"
     gene            complement(3770..4084)
                     /locus_tag="HUK62_RS18850"
                     /old_locus_tag="HUK62_18850"
     CDS             complement(3770..4084)
                     /locus_tag="HUK62_RS18850"
                     /old_locus_tag="HUK62_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_176538642.1"
                     /translation="MSMVRYSRKELNEKFSDKQDAEIERLLAKGTIPDDQLDLSDIPEV
                     ADWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(4071..4358)
                     /locus_tag="HUK62_RS18855"
                     /old_locus_tag="HUK62_18855"
     CDS             complement(4071..4358)
                     /locus_tag="HUK62_RS18855"
                     /old_locus_tag="HUK62_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_130114512.1"
                     /translation="MKQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDKYEHG"
                     /name="BrnT family toxin"
     gene            complement(4694..5296)
                     /locus_tag="HUK62_RS18860"
                     /old_locus_tag="HUK62_18860"
     CDS             complement(4694..5296)
                     /locus_tag="HUK62_RS18860"
                     /old_locus_tag="HUK62_18860"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="WP_119686775.1"
                     /translation="MVKELELFDHVVLNKSNIRLIPLSFEHESGLMEACKDGQLWKLRV
                     TSTPHYSEVHQYIETALIQKQEGTRYPFVVIEENSGKILGTTSYHDVLLNVKRLEIGYT
                     WYAKSVQRTHVNTTCKLLLLQFAFEQLNIETVGLRTDIFNIKSQRAIERLGAKKDGIIR
                     GHALRKDGTTRDTVMYSIIKSEWTNIKAHLIDLLDNY"
                     /name="GNAT family protein"
     gene            complement(5537..6052)
                     /locus_tag="HUK62_RS18865"
                     /old_locus_tag="HUK62_18865"
     CDS             complement(5537..6052)
                     /locus_tag="HUK62_RS18865"
                     /old_locus_tag="HUK62_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_086179909.1"
                     /translation="MKTLTVLELSKLYNINRQTIYNNIKKGILSKNSENKIDLAEAIRV
                     FGEPVKKQDVKEFVKVDSPNSAEVLLLRQQIDMLKNQLNDAKDRESFYQNQIETMQRLL
                     EAPKAPKMEDPTPVVPPQETIEVPVPEQATEVKTEIQTQNKRIPVPEHVEQEPEKRGFW
                     SRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6131..7060)
                     /gene="repM"
                     /locus_tag="HUK62_RS18870"
                     /old_locus_tag="HUK62_18870"
     CDS             complement(6131..7060)
                     /gene="repM"
                     /locus_tag="HUK62_RS18870"
                     /old_locus_tag="HUK62_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_176538643.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARQTGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDVNPKTGKDRSFHCRWVDKIG
                     YEAQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PVFDLSIFRQQLGVEPHQYKTMSNFKTYVLDFALNQINELTDIIAKYEQHKKGRTISGF
                     SFTFRQKKDTSKKVITSENPSDYFSKITDPQRHLFANKLSRLPEMNTYSQGTESFEQFA
                     VRIAEMLKDAQKFEELFPYLQKVGFTPA"
                     /name="repM"
     gene            complement(7925..8302)
                     /locus_tag="HUK62_RS18875"
                     /old_locus_tag="HUK62_18875"
     CDS             complement(7925..8302)
                     /locus_tag="HUK62_RS18875"
                     /old_locus_tag="HUK62_18875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086179911.1"
                     /translation="MLPKIKSKAFIRLLPQIFELREQGYSYEEVTAILEKEYGLILSVN
                     QFSVYLARYKNAHTSKVKKQVEISPARKQHPESKQDPIKPDSNTKDYVFGSPEHKAAIQ
                     KETEENFRKSDMRIGLGLKYP"
                     /name="hypothetical protein"
ORIGIN
        1 tacagaaagt ggcgcagtgg ataatttaag tctggaaatg ctgctgatgc ttcgggcaat
       61 ggctaagcct gaacagttaa aaatggcaca tgctcggatg cgcgagaagg attttcctat
      121 tctcaatttg caggattact aatctccaga tcagatccat ctcataggat tcatttcaac
      181 ggaattaaat atggctattt ttctggttcg acatgctgaa agtgaagcga atatcaatgg
      241 aaagacgctt tctcatgctt caattgcttt aagtgagcat ggtcatagac aggctcagga
      301 tttatgctcc aaactgcctg taatcgatca tgtgatcgtg tcgaagtatt tgcggactca
      361 gcagaccgca gcgccaatat tggaaaaata cggcctttca ttagaggttg atgagcattt
      421 acatgagttc agctacttgt cagagcgtaa gtgtgccaat accaatctga atgaccgtaa
      481 ggcatgggta aatgcgtact gggagaaaat ggattatcag cacagggatg ctgacgatgc
      541 agagtctttt gagaatctgt atcttcgggt acaggctttt caggaaaagc taaagatctt
      601 gacccctcac tatgtgaaga agaatctggc tgtattcagt catggacagt ttctacagct
      661 tctcattatg cagatccagc agcccagatc tcttaccaag gaattgatgc agcagtttag
      721 atcggacttg gttcgccagc cgatcagaaa tactgaggtt ttcacctact gaacagaatt
      781 tcgtatatga gattttatgt taaataaaaa gatagcttga tgactatctt tttatttaac
      841 aatcatagct atagctctaa agttaaagaa gtgtgctctg aaattttact ttgtaataga
      901 gcgattaaag cctctaactc tacttttgct tcagttttta aaccaaggca aacccctata
      961 ccatcagcat ctgtacttag tcttttactc agagagatca tgaaatcttg tttcgagtta
     1021 tcatttttaa gctgatacgc atgtaaatgg aatgagtcag caatatcaac attctcgttt
     1081 agatgatcaa atgagaattc ttttttcata tagtctaata gtacatcttg aatgacgagt
     1141 accatctttt tattaatatg ttcgaaagtt tctaccttat ggtgcatttg aacgagaatg
     1201 gttttagctg tcattttcca attcatgcca aaaggttttt tctcgacttc agtgttagca
     1261 tcaataattt ttagttcttt caacaagttt tgacgttctg gccaaatcgt tcctgtagta
     1321 tctaatgttt gtaattcaat acctacaaag tctttaactt ttccatcttt tgcagaaata
     1381 agaaaaaaat ctacattccc cccaggaatc ttaacttctg gtactaaatg caattcattg
     1441 ccaggtgtgt gacccgtgag caaatgaaga cagtctataa atatctgctt tctttctaga
     1501 aggcgattag ggcaaataat agttgatact ttttctgcct ttttatctat aggactagaa
     1561 tattgtaagg tgcatgttcc tatagcaata gagggatctg actttcttgt tttataacac
     1621 ttttttttag aataaggaca tgtctgatct gctatggctt taattaaatc actcggctca
     1681 tgagtagttt tcaaaccaaa aatttctaca attttactca ttaattactc tacgtattgc
     1741 tgtatctaga tagctttttg aaatatctat accaatagat tttctattca aaattttagc
     1801 tgctatacag gtggttcctg ttcctacaaa tggatctaaa acgattccat catttggaca
     1861 ggtagctaaa ataggaattc tacatagatc aattggataa ggagcaaaat gctctttatt
     1921 ttttgaaaaa ctttcaggca aaatttccca tacatcgtta ggtttactgc catcagggtg
     1981 gtatttaaga aaataaaagc cttttgttaa taactcttta gcacgaccag atactttatc
     2041 actgttagaa tgagtcgtac ggttagcacc tcgtattacc atgcgaaaat ctgaatactt
     2101 tccatcttgt atcgacttca gcatttcttc taatgcttta aaagcattat ttttttcttc
     2161 ctcgctgagt tccgttgata actcaatttt tcttttatac ttaactcctg acactccagt
     2221 agccgagacg atactgccat ttactacctt actttgtctt ggttttgatc tgattgcatc
     2281 agtactataa aaatagttag caggatcttt aacaaagtga aaaatggttt catgaacatt
     2341 ccctaactta tctttagagt tatccattcc acctttaacc ttgttccaaa ttacttcatt
     2401 acgaagaacc caattttgtt catcaattaa tttaaatgcg aatctccaag gcatacctaa
     2461 aagatgttta tttttatata catctcctag atttagccaa aaagaacctg ttggttttag
     2521 aacccgtttc agttcacttg taatctgtaa taggttgtta aagaaatcat ctaaagtttt
     2581 ttcaagacct ataccaccat tttcatattc tctttggctc caatatggag gtgaggtaat
     2641 gattgtatct actgattcat cgggaatttg ttttaggaca tctaggcaat cacctaataa
     2701 aaataatgga ttagtgtcat cattcttttt atattcctca actttaggcc caaagttatc
     2761 tccaatgatg ggaatctctt gaaaaaatag atcgggattt tgagggaaat tcataacaaa
     2821 ttacatatta ggagaacata agtattgtag gtgattttgg gatagtttag agactattat
     2881 tgatgggaat aagaccattc agatattgag agttttggca tgattacaga agaattcaat
     2941 acagttagag catttctaga aaaaatgtta gaacaaaacc cagaccataa ggggttttta
     3001 gacgcttatg tcaaacttat agaagctaaa agtaagtttg acctagagac gaataaggca
     3061 ataattgaaa aagaaatacg tcactcagag ttaaactatg acttattgaa aacccaagac
     3121 actaataatg ctaatgttca tatgaatcaa aatacgaact gggctgatgt aaataaaacg
     3181 tttaattcca attatcatca gacacaacaa ggctatcaca atcaagcatt tggtctaatg
     3241 aataatgcac ttacgaatag agaccttttg agatagtgag atttatagac tcttattgga
     3301 ggaggagata aaccttatct ccccttttac tttgcataat agtcattctt tagggattta
     3361 tactatattt ttattttcta atattcagtg aataatcatc gtcttgttgt ctgcgcttta
     3421 gtctatattc atcatcttgt tttttcgatt cgagttcaga attatctcct tgttttctac
     3481 gttcgaggcg gtaatcattg tctgcttgct ccatcttcaa attattttct aaatgagctt
     3541 tttctaattc tattaattta ttgatgaaat ctaacttctt gtctattaat aatactttat
     3601 tctcaaatac atccgtttca tcgatcaatt tatctaaaag agacaagaag ttaagttcgt
     3661 gatcaagtaa tgcaaatttc attttgagtt ctccatagtt taaagatttt ctaaaattaa
     3721 cataatacgc gttatacgca gtcaaaaatg ggagcctaag ctcccatttt tactgatgat
     3781 tttttaactc ttttagcatg gcatcccgca agatttcatt cattcgtgtc tgataacctt
     3841 taccttgtgc tttgaaccat gcaagtacgt cagcatctaa acgaatagat gtttgttgct
     3901 ttactggacg atagaattga ttttggcgta cagcattact ccagtcggca acttcaggaa
     3961 tatctgagag atctagctga tcgtcaggaa tcgttccctt agcaagcaag cgttcaattt
     4021 cagcatcttg cttgtcgctg aatttttcat tcagttcttt gcgtgagtat ctaaccatgc
     4081 tcatatttat ctcgctccgc tttagttacc tgtcttgcac taatgattcg tatgatttca
     4141 cagtcatctt catcaaagat ggtatgagct actaagagca ttagtacgcc ttttactttt
     4201 ccaatggttt gccaacgttc ttcaccatcg gtatgtctgt cttggattga gatcctcagt
     4261 ggatcttcaa aaacaaggct tgccgtttcg aaagagacat cgtgtttttt ctgattcttt
     4321 cgattctttg cctcatccca ttcgaaatac tgtttcataa aaaacattca aatttgtata
     4381 tacaatagtg tatcacaaaa ttgtatcact caagttgaga agcgtaattt ttagtcaaaa
     4441 ttattggccg aaagtgacaa atttgttcaa taaaaagccg acttgtttca aaatcggctt
     4501 tttttgcatt tttagatttt tctggtttta ttaagtattt gatatttatg ttaatataca
     4561 agtgtacttc gtataatcgc cattatgtta aatggatggt gaattggtgg aaaattccag
     4621 tttttataat tgcgaataag agtttttatg ttaaatcata atcctaatgt attagtgtaa
     4681 taaaatctta tagttaataa ttatctaata aatcaattag atgtgctttt atgttagtcc
     4741 attcactttt gataatactg tacattacgg tatctcgagt tgtaccatct ttacgtaaag
     4801 catgacctct aattatccca tccttttttg cgcccaatct ttcaattgct cgctgacttt
     4861 taatattaaa aatatcagtg cgaagtccaa ccgtctcaat atttaactgt tcaaaagcaa
     4921 attgtagtaa tagcaactta catgtcgtat tgacatgcgt acgctgcact gattttgcat
     4981 accaggtata acctatttct aagcgtttca cgttcaaaag aacatcatga taactagttg
     5041 tacctagaat tttcccagag ttttcttcaa tgacaacaaa tggataacgt gtaccttcct
     5101 gtttttgtat tagagctgtt tcaatatatt gatgaacctc agaataatgt ggtgttgatg
     5161 tcactcttaa cttccatagc tgaccatctt tacatgcttc cataagccca ctttcatgtt
     5221 caaaagataa gggaattaaa cggatattac ttttattcaa aacaacatga tcaaataatt
     5281 ctaattcttt cacaatactt tccctttacg acatttgagc tgaatacaag tggttctatt
     5341 aagttaataa tttctaaaat taacataata caccttatac gaaatgtgtt ataattttcc
     5401 ttagaaatca atgctgtagt ttctagttta tagcccaatc acacccgatt gggctttttt
     5461 tatgattttt cacgttccac gcctattatt tgatcaatgt tccacaactt tgttcatatc
     5521 agttaaacaa tcgtggttaa tcatagggtc taaaaaaacg gctccaaaag ccccttttct
     5581 ccggttcctg ttcgacatgc tccggcacgg ggatccgctt attctgcgtt tggatctcag
     5641 ttttaacctc agttgcttgt tcaggtactg ggacttcaat cgtctcttga ggtggtacaa
     5701 ctggtgttgg atcttccatt tttggtgctt taggagcttc tagtaggcgt tgcatcgttt
     5761 cgatctgatt ttggtagaaa gactcacgat ctttagcatc attaagctga tttttcagca
     5821 tgtctatttg ttgtctaagc agtaaaactt ctgccgaatt tggactgtct acttttacaa
     5881 attcttttac atcctgtttt ttgactggtt ctccaaaaac acggatagct tctgctagat
     5941 caatcttatt ctcagaattt ttacttaaaa ttcctttctt tatgttgttg taaatcgttt
     6001 gtctattaat gttgtaaagc ttggaaagtt caagaactgt aagggttttc attcagtaaa
     6061 cctatgccta aacttgtaaa aagttactgt ctattagact gtctaataga caaatgatca
     6121 agtttaaatt ttaggcagga gtaaagccaa ctttttgaag ataagggaat aattcttcga
     6181 atttttgggc atctttaagc atttctgcaa tgcgcacagc aaattgttca aagctttctg
     6241 taccttgaga atatgtattc atttcaggaa gtctagaaag cttattagca aataggtgac
     6301 gctgtggatc ggttattttc gagaaataat ctgagggatt ttcacttgtg atcacttttt
     6361 tagaagtatc ttttttctgt ctaaaagtga acgaaaatcc tgaaatagta cgtcctttct
     6421 tatgttgttc atacttagca atgatatctg ttagttcatt tatttgattc aatgcaaagt
     6481 ctaaaacata ggttttaaag ttactcatag ttttgtattg atgaggttca acgcctaatt
     6541 gttgtctaaa gatagacaga tcaaaaacgg gagtttttcc tgctgacctc cattggatca
     6601 gtaattcata gagtcgaata gcataagaac tagttaggcg tgagacctgt tctaactcat
     6661 atttagtaaa attctcttct agtctggtta taagaggaac gatatcttga gtgaatctta
     6721 aaaaaacaat tcccgactga gcctcataac caattttatc aacccaacgg caatggaaac
     6781 tacgatcctt acctgtttta ggattaacat catggtatgt gacgtatcta tcgaagaggc
     6841 tttttgatgc atctctaagg actgtatagg cagtatgttt ttcaacattg aacgtattta
     6901 tatagctact ggcatgtacc tctaataagc tattttctgt tatcccatga cctgtttgtc
     6961 ttgcttctgc tatagctaga agaattaaac gctgctcaac agtatctaac gtatagctag
     7021 cttgaattaa ggcattatcc ttaacaatta attcactcat ttctaataat aaatttataa
     7081 ttaaactagg ttgtacaata gactatttaa ataaacgtgt ctattaaaat tattaaggtc
     7141 gataaaccta gctttaaggt cgataaacct agctttaaag gtcgataaac ctagtttaat
     7201 aggtcgataa acctagttta ataggtcgat aaacctagtt taataggagc tataacgctt
     7261 tctacacaag gctttcagct tacctaaaag catttaaaaa ctttaaaata attaaaagcc
     7321 caaggaagga aaaattgccc ttggttttcg cttcgtttca ctcgctcaaa ctctattgaa
     7381 tctcgctttc gctcgattcg tcggggcaat tttttgcaaa tactttctaa aattatgtaa
     7441 aaagcaaaag cgactatgac ttcgcttcgc tcagaagttt ttttgctcgc ttcgctcgat
     7501 ctaggtgcaa atatttacat gtgatttacg cttatttagg cttcacagcc taaaaagctg
     7561 tttttcaggg agtctagacg cgacttttcg ttgtgtcgct cgtagacact cgctaaacaa
     7621 actattgatt attagtagtt atatttttat tcactgacat aaaaaatgat atccattaaa
     7681 tatcggaaaa tgtcattgta taagacttta agccttatac tgtaagggtt acagaactct
     7741 gaataagaaa taaattttat tctgctcaaa agatcgtagt gaggactttc gatttcatat
     7801 ttcaataata gaaaaaagaa tgctcctatt tggctttatt tggcgattta agccaatttt
     7861 ttaattttcc cacagttcta tgatcttttt taaaataatg tcttctatgc gatctggtgg
     7921 ctttttaggg gtactttagt cctagaccaa tacgcatatc ggacttacgg aaattttctt
     7981 cggtctcttt ttgaattgct gctttgtgct caggtgaacc aaaaacataa tccttcgtat
     8041 tgctatcagg tttgatcgga tcttgtttac tttctggatg ctgttttcga gcaggggata
     8101 tttcgacctg tttttttact ttggaagtgt gggcattttt gtaccgtgcc agatagactg
     8161 aaaactggtt gacgctcaga atcagtccat attctttttc taaaatggcg gtcacttctt
     8221 cataagaata gccctgttct ctgagttcaa aaatctgggg taaaagacgg ataaaggctt
     8281 tagatttgat ttttggcagc atgactttag atttcagtgg aataacttat tttgatagta
     8341 aattgttagt gctttggttg caaattgata tgttttcagt cgtaaattca taattttgat
     8401 acctaattga tatccttttg ataacttttt gatagtaaat cagtagtaaa ttgatatata
     8461 cgcccttttt cctgtttttc gctctaaatt ctgctctaaa ttgaagatgg atttttgacg
     8521 atttttaggt atacaccata cgcacatagt catgatgcta aaaaatcttc gattttttat
     8581 ttaaaagcaa agacttatat ctattttact tgtctttcat catgttttta gacgacgcca
     8641 aatgtcacaa atataaacat aaatatttgt tttatatatt tataattgtc acgtatttgt
     8701 cacagatcaa aatgaaatga gtaatgataa tcgggttacg atcaggctgt ccaaagagca
     8761 gtatttcttg tacgaaatga gagcggctgc caagggaatg cgtgtatcga cttatgtgaa
     8821 aagtttgctg gatgagtgtg agtcaagctc gtcaaatcag ctcgcagaac tgctgagatt
     8881 gtttaaaagt ctggatgaaa aaacgtcatt gattacggaa gttttaatgg cccagaaaga
     8941 tgaaaaacca gcgca
//
