LOCUS       NZ_CP055290            10333 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH8, complete sequence.
ACCESSION   NZ_CP055290
VERSION     NZ_CP055290.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10333)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055290.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH8"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            complement(join(9484..10333,1..107))
                     /locus_tag="HUK62_RS18765"
                     /old_locus_tag="HUK62_18765"
     CDS             complement(join(9484..10333,1..107))
                     /locus_tag="HUK62_RS18765"
                     /old_locus_tag="HUK62_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_085064994.1"
                     /translation="MFFCNLEHNSKNPPQKGGKNNKPRTAKERFHYITRTAQFAQHKDH
                     VHEQLEFVCSGNMPSFAEGNPEEFWQASDLYERKNGRVCSSLVVALPKELTSEQRIELA
                     EQFIQEFADRYQYPYSCAIHHHVGSLGGQEQPHLHLIYSERHVDGIERTPEQFFKRYNP
                     EQPEKGGAQKLTADVLGMGKAQLQLYRQKTEELINDSLQRYAPTKVLEIRGLKVEVPNE
                     VSCLSNEDYNKKYDTNLQDVPMMNKALRFAKESDPVRYQQKQDMIVEINRLRAENRYEL
                     YKPYYEVELNKQKLLEQEKQKQTQEKTKGFDGPRFGF"
                     /name="MobA/MobL family protein"
     gene            461..1219
                     /locus_tag="HUK62_RS18770"
                     /old_locus_tag="HUK62_18770"
     CDS             461..1219
                     /locus_tag="HUK62_RS18770"
                     /old_locus_tag="HUK62_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_130136800.1"
                     /translation="MATRGRPAKILTTTDLVELEKFLADLPYLKKNQNLAISLLHSKEF
                     SELDEKDLSLLKIVHREKIQFQQRQAMITQIQIKQRNQQQLLANEIEILQLLEQEQDQD
                     TFFRLDRALESYQKIEKAALENRIRLENEHKRDILNKTNKKQTEAQKKRNAENQLKYAL
                     GGIILSIWKHAEWDIDPNNLKTVENRIKSSFLSKSKIQKSALYKEAFAMTQDHQEAQKL
                     FFLALEKLPTYNIQQEDFHKVLLKKVRESQ"
                     /name="hypothetical protein"
     gene            1936..2871
                     /gene="repM"
                     /locus_tag="HUK62_RS18775"
                     /old_locus_tag="HUK62_18775"
     CDS             1936..2871
                     /gene="repM"
                     /locus_tag="HUK62_RS18775"
                     /old_locus_tag="HUK62_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004759367.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_055414480.1"
                     /translation="MSKELVVKTNRLNQAFQTLSLSEFHIVQLAIVDARHTGTGLSTDT
                     PLRIDALRYAEIFGTTRQNAYQRMKEAEDSLFNRRFSFFDEDGKLVKSRWIQQVKYLDD
                     EGAIELVFTLAVVQGISKIDGIKDFFTQYLLSQTAQLNSTYSARLYELLIQWKAVGKTP
                     VFELETFREQLGIGVNEYKRMDHFKTRVLDLAITEISEKTDIEATYLQHKKGRSISGFS
                     FSFTQKKSKNTSNENLKNSENLDLFSKKMTDAQRHLFANKLSELPDMNKYSQGTESYPQ
                     FAVRIAEMLQDKEKFEELLPYLKKVGFSTK"
                     /name="repM"
     gene            2932..3489
                     /locus_tag="HUK62_RS18780"
                     /old_locus_tag="HUK62_18780"
     CDS             2932..3489
                     /locus_tag="HUK62_RS18780"
                     /old_locus_tag="HUK62_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_228258384.1"
                     /translation="MNTTKFSVMDASKAFKKSRTTIYEAIKTGELSRDHDGLIDLSELI
                     RVYGNPIGVQSSTRTEHAHKDVHVHVQSEIEHVLKDQISLLKNQLDLANQREKSLMQHI
                     EDLTHRIEFKGQLEQSKTELDERKVTTHSEMTGNSRPEPEPKHDGLTTPVEAENKRIPV
                     PEHIEHEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            3730..4332
                     /locus_tag="HUK62_RS18785"
                     /old_locus_tag="HUK62_18785"
     CDS             3730..4332
                     /locus_tag="HUK62_RS18785"
                     /old_locus_tag="HUK62_18785"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="WP_119686775.1"
                     /translation="MVKELELFDHVVLNKSNIRLIPLSFEHESGLMEACKDGQLWKLRV
                     TSTPHYSEVHQYIETALIQKQEGTRYPFVVIEENSGKILGTTSYHDVLLNVKRLEIGYT
                     WYAKSVQRTHVNTTCKLLLLQFAFEQLNIETVGLRTDIFNIKSQRAIERLGAKKDGIIR
                     GHALRKDGTTRDTVMYSIIKSEWTNIKAHLIDLLDNY"
                     /name="GNAT family protein"
     gene            4668..4955
                     /locus_tag="HUK62_RS18790"
                     /old_locus_tag="HUK62_18790"
     CDS             4668..4955
                     /locus_tag="HUK62_RS18790"
                     /old_locus_tag="HUK62_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_176538634.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQNRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            4942..5256
                     /locus_tag="HUK62_RS18795"
                     /old_locus_tag="HUK62_18795"
     CDS             4942..5256
                     /locus_tag="HUK62_RS18795"
                     /old_locus_tag="HUK62_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_176538635.1"
                     /translation="MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLNLSDIPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            5441..5887
                     /locus_tag="HUK62_RS18800"
                     /old_locus_tag="HUK62_18800"
     CDS             5441..5887
                     /locus_tag="HUK62_RS18800"
                     /old_locus_tag="HUK62_18800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538636.1"
                     /translation="MIICAHPLDIVILIFFKSIAIDKINFIELLELKMTKIFSDDYNEF
                     VFPEEFTFTNGETVNTDDISIQPVRDEEENRILAFNVSTDLEGAGTVSNHLIVQPFNEI
                     QVGLGRYDEDPDEYEHPDDSSYDTVEYVSDETTAQIEINDIEEY"
                     /name="hypothetical protein"
     gene            complement(6031..8643)
                     /locus_tag="HUK62_RS18805"
                     /old_locus_tag="HUK62_18805"
     CDS             complement(6031..8643)
                     /locus_tag="HUK62_RS18805"
                     /old_locus_tag="HUK62_18805"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_176538637.1"
                     /translation="MLNESEGSGQKSIYLILLKTLKKLYFQYNKSILQNKLEVKMSFFD
                     EIFLDNDPIKINNINELYNSLDAKSSHSSLRVIQQECIDILDSKLDEQGVILKLSTGSG
                     KTTIGLLYLKYYAQKYNEPVLFLVPTLQLAEQVIAEATKLGIQSTLYSKGHRTVSPEAM
                     CGDEIIVSTYEKLFNGFSTFKTYGIQPSAIVFDDAHTGMDIIKKQFSIISSGEIYTELL
                     SIIKGSCKKHLGIQWLEFETDGKPFEVPYWIWQSKIEEISKLLNKHKDHKSLKFNYPLI
                     KNELNFCRCIFSQANFEISPNILNIKSITPYYESKHKLFMSATFSDSDNFIRFLGLEKD
                     IVKNMVAPTSDKGTGERMILVPSIISPDLDKNKVIGLCADLSKQTTVFVLTSSINQAEV
                     WVNEGAIFLNSENINEALSDLSSQINKSGFYVMAQRFEGLDLADDLCRILVIDEIPFGE
                     SIVDNYDTECLGNITGFGGKNIFRLEQGMGRAVRSHVDYSVILLVGSDLSSFIAHKDCR
                     TALSPETNCQLDLGIRVSKQIAKNQKDPIENIKKTIASCLSRDLAWKNGYQSEMKKVKK
                     TEFSYDEKLLNLAYAERKFYEDAYINQFHLSTEIFNNAINLLSDSKYKGKMLENFARIV
                     NFTDELESMKIQNRAIQLYPRAIRPLTVYPRKLFKEPTNSAINIEKIIKDYNNLEGILI
                     QLQKIKSDFGFEDVKSSKVENNFKLLGYFLGADSINPEKEYKEGPDVCWYLDNSVYVIE
                     IKHNQVESLHKTDSGQLSDSCQWAQKNYPPMANIIPTTLTNVTFFDRDAHYLEDTVVIN
                     ELRTLELLDVLIRFYEHIIRESKNTAQEITSELNQYGLNNNTIFQKYFKKFNSIMRKK"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(8713..8937)
                     /locus_tag="HUK62_RS18810"
                     /old_locus_tag="HUK62_18810"
     CDS             complement(8713..8937)
                     /locus_tag="HUK62_RS18810"
                     /old_locus_tag="HUK62_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005155906.1"
                     /translation="MPYEEFQRLIGKSGLSIKEFAALLDMNANSITNYKKNGKVPTTIA
                     VIAVIISDMKDDGLDFYPIFEKVRAYRDQ"
                     /name="hypothetical protein"
     gene            9116..9376
                     /locus_tag="HUK62_RS18815"
                     /old_locus_tag="HUK62_18815"
     CDS             9116..9376
                     /locus_tag="HUK62_RS18815"
                     /old_locus_tag="HUK62_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126039475.1"
                     /translation="MGNLNLINHLYLSENGRKIGTQLIKDFSINRSYNLGLFLNVNKCF
                     DDREATLVWTQHYLDQHIDDDYEDVKRAFLAFFPDGAFMQF"
                     /name="hypothetical protein"
     Typing          1937..2811
                     /name="rep_cluster_1261"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.949"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          complement(9488..10332)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.489"
                     /coverage="89.0"
                     /evalue="8.770000000000001e-167"
ORIGIN
        1 gttcgagtga tgtagtgaaa gcgctctttg gccgttctag gcttgttatt tttaccacct
       61 ttttggggcg gatttttact gttgtgttca agattacaga agaacatatt tgcgtctagc
      121 cctcacgtta tttttgatgt tgtaacgaca ctttttttgc agcaacacac atttttgatg
      181 ttgtaacggt agatcgctat tgcagaaaca aatgtcaaaa atgcagcaac agtactctga
      241 tagatacaaa tcaataactt acgtctctaa tggcctgcgg agcacgagga gaagtgtttc
      301 taattcgata taattgcgtt agtaattata tcgaattaga aacacggaaa cgagcttaaa
      361 gatttaaata aatgttctca cattaattta aatcttttcc cgtgatggca tatgctagac
      421 tgaagcatca gaattacacc tattttcgaa tggagcatag atggccacac gcggaagacc
      481 agcaaaaata ctcacaacaa ctgacctagt agaattagaa aaatttctcg cagatttacc
      541 atatctaaag aaaaatcaga acctagcaat ctcgcttctt cacagcaaag aattttcaga
      601 gttagatgaa aaagacctca gccttttaaa aatagttcac cgtgaaaaaa ttcagtttca
      661 acaaagacag gcgatgatca ctcaaatcca aattaaacaa agaaaccaac agcaactatt
      721 ggcaaacgaa atagaaattt tgcaactact tgaacaagag caagaccagg acacattctt
      781 tcgcttagat cgagcactag agtcttacca aaaaatagaa aaagcagctc tagaaaaccg
      841 gatccgttta gagaacgaac ataaacgcga tattttgaat aaaaccaaca aaaaacagac
      901 ggaagctcaa aagaagcgga atgctgaaaa tcaacttaaa tatgcgttag ggggaataat
      961 tttatcgatt tggaaacatg ccgaatggga cattgatcca aataatttaa aaacagtgga
     1021 aaatagaatt aagagtagtt ttctttcgaa atccaaaata caaaaaagtg cgctttacaa
     1081 agaagccttc gcaatgacac aagaccacca agaggcccaa aagctcttct ttcttgcact
     1141 agagaaactc ccaacctaca acatccagca ggaagacttc cataaggtat tgctaaaaaa
     1201 ggtgcgagaa tcgcaataaa tgctcatttc agcgcaattt agacttaaac catacaaacc
     1261 ttggtcttaa atccaatcat cgctcaaaac gcaaatgatg gctttataga acccataaaa
     1321 ataacccatc gccttttatg cttttaatac ctaataggta tcaaaagagc gagtgtctac
     1381 gagcgaaccc ctaaaattcg cgcctagact ccttgaaaaa ccgttttttt aggctgtgaa
     1441 gcctaaaaga acctagatca aatgctgaaa atggcgccta gatcgagcgt agcgagtaaa
     1501 aaagctttat gagcgaagcg aattccgagt tgcttttgat cttgcttttt ctcaaagtca
     1561 taaaggtatg agctaaaaaa ttgcccctcg aatcgagcgt aagcgagatt caatagagtt
     1621 tgagcgtagc gaaaaccaag ggcaattttt ctttgccagg gcttttaatt atttttaaga
     1681 ttttaaatac ttttaactat ccttaaagcc tttatttaaa aggctttcag agcttatatc
     1741 cccacgttta gttacctata tccccacgtt tagttaccta tatccccacg tttagttacc
     1801 tatatcccca cgtttagtta ccacttcccc acgtttagtt acctatatat attcattaaa
     1861 atcccccaaa ataaatattt gtggttatac ccacattgat ataatctgtg ggtataagaa
     1921 cttttaatca aacttatgtc aaaagaatta gttgtaaaaa caaatcgctt aaatcaagcc
     1981 tttcagactt tgtccttatc agagtttcat attgttcaac tagctattgt tgatgcaaga
     2041 cataccggta caggccttag cacggatacc ccattgcgaa tagatgcttt gagatatgct
     2101 gaaattttcg gaacaacgag acagaatgct tatcagcgaa tgaaggaagc tgaagactct
     2161 ttatttaatc gcagatttag tttctttgat gaagatggaa aacttgtaaa aagtagatgg
     2221 attcagcaag taaaatattt agatgatgaa ggtgctattg aactagtttt tacactagcg
     2281 gttgtacaag gcattagtaa aattgatgga ataaaggatt tctttactca atatctacta
     2341 agccaaacag cacaactaaa tagtacctac tcagcaagac tatacgagtt acttattcaa
     2401 tggaaagctg ttggtaaaac gccagtattt gagctagaga cctttagaga acagcttggg
     2461 attggtgtta atgaatataa gcgtatggat cacttcaaaa ctagagttct tgatttagct
     2521 attacagaaa ttagtgaaaa aacagatatt gaagctactt atctacagca taaaaaggga
     2581 cgatctatat caggattttc tttttcattt acacagaaaa aatctaaaaa tacatcaaac
     2641 gaaaatctaa aaaattccga aaatttagat ctcttttcaa aaaaaatgac cgatgcacaa
     2701 cgtcaccttt ttgctaataa actttctgaa cttccagaca tgaataaata ttctcaaggt
     2761 acagaaagct acccacaatt tgcagttcga attgctgaaa tgctacaaga taaagagaaa
     2821 tttgaagaac ttctgccata tcttaaaaaa gtaggattta gcacaaaata aacttcatgt
     2881 tcaggtagga tgttcatcta aacatccttg tacatgcact gagggattac attgaacaca
     2941 acaaaattca gcgttatgga cgcgagtaaa gcttttaaaa agtctcgaac aacaatatac
     3001 gaagctataa aaactggtga attatcaagg gatcatgatg gactcataga tttatctgaa
     3061 cttatcagag tttatggaaa tcctatcggt gttcagtcca gtacacgtac tgaacacgcc
     3121 cataaggatg tacacgtaca tgttcaatct gagattgaac atgtattgaa agatcagatt
     3181 tctctactca aaaatcagtt agatctagcc aaccaaagag aaaagtcttt gatgcagcat
     3241 atagaagatt tgacccacag aattgagttt aaaggtcagc ttgaacaatc aaagacagag
     3301 ctagatgaac gtaaagttac tactcattct gaaatgacag gaaattctcg accagaacca
     3361 gagccgaaac atgacggatt gactactccg gtcgaagcag aaaataagcg gattccagta
     3421 ccggagcaca ttgaacacga accggaaaaa aggggctttt ggagccgttt ttttagaccc
     3481 tatgattaac cacgattgtt taactaatat gaacaaagtc gtgaaacatt gatcaaagaa
     3541 taggcgtgga acgtgaaaaa tcataaaaaa aacccatctt ggggaagatg ggctataaac
     3601 tagaaactac agcattgatt tctatagata attataacgc atttcgtata aggtgtatta
     3661 tgttaatttt agaaattatt aacttaatag aaccacttgt attcagctca aatgtcgtaa
     3721 agggaaagta ttgtgaaaga attagaatta tttgatcatg ttgttttgaa taaaagtaat
     3781 atccgtttaa ttcccttatc ttttgaacat gaaagtgggc ttatggaagc atgtaaagat
     3841 ggtcagctat ggaagttaag agtgacatca acaccacatt attctgaggt tcatcaatat
     3901 attgaaacag ctctaataca aaaacaggaa ggtacacgtt atccatttgt tgtcattgaa
     3961 gaaaactctg ggaaaattct aggtacaact agttatcatg atgttctttt gaacgtgaaa
     4021 cgcttagaaa taggttatac ctggtatgca aaatcagtgc agcgtacgca tgtcaatacg
     4081 acatgtaagt tgctattact acaatttgct tttgaacagt taaatattga gacggttgga
     4141 cttcgcactg atatttttaa tattaaaagt cagcgagcaa ttgaaagatt gggcgcaaaa
     4201 aaggatggga taattagagg tcatgcttta cgtaaagatg gtacaactcg agataccgta
     4261 atgtacagta ttatcaaaag tgaatggact aacataaaag cacatctaat tgatttatta
     4321 gataattatt aactataaga ttttattaca ctaatacatt aggattatga tttaacataa
     4381 aaactcttat tcgcaattat aaaaactgga attttccacc aattcaccat ccatttaaca
     4441 taatgggcgt tatacgaaac gcatataaaa atagttttat atttcaaaaa gttatgctat
     4501 aaaaaatcgg ggacgcaaac ccgaaaagcc gacttggaaa caagtcggct tttttatgag
     4561 cggttttgga atgttttgcc aataattttg actaaaaatt acgcttctca acttgagtga
     4621 tacaatttta tgatacatta ttgtatatac aaatttgaat gttttttatg gaacagtatt
     4681 tcgaatggga tgaggcaaag aatcgaaaga atcagaaaaa gcacgatgtc tctttcgaaa
     4741 ctgcaagcct tgtctttgat gatcctctaa ggatctcaat ccaaaacaga cacaccgatg
     4801 gcgaagaacg ttggcagacc attggaaaag taaaaggcgt actaatgctc ttagtagctc
     4861 acaccatctt tgatgaagat gactgtgaaa tcatacgaat cattagtgca aggcaagtca
     4921 ctaaagcgga gcgaaataaa tatgagcatg gttagatact cacgcaaaga gctgaatgag
     4981 aaatttggtg agaagcaaga tgctgaaatt caacgcttgc ttgctaaagg cactgttcct
     5041 gatgatcagc taaatctatc agatattcct gaaattacag actggagcaa tgctgtacgc
     5101 cataatcagt tctaccgtcc agtgaagcaa cagacttcca ttcgtttaga tgctgacgta
     5161 cttgcatggt tcaaagcaca aggtaaaggt tatcagacac gcatgaatga aatcttgcgg
     5221 gatgccatgc tcaaagaatt aaaaaatcat cagtaaaaat gggagcttag gctcccattt
     5281 ttggcttcgt ataacgtgta ttatgttaat tttagcgaaa ctttagtatt agaaagagct
     5341 tagtaattca ctcaaatgtg tagatattaa taaaatacat ggtactaaat taactttata
     5401 ctatgcattt tttgcacctg ttgacagcaa attttagcat atgattatat gtgcacatcc
     5461 attagatata gttatcttga ttttcttcaa aagtattgct attgataaga taaattttat
     5521 tgaactgctt gagctaaaaa tgactaaaat attttcagat gattacaatg aatttgtttt
     5581 tccagaggaa tttacattta ctaatggtga aactgttaac acagatgata ttagcatcca
     5641 gccagttaga gatgaggaag aaaatagaat actagctttc aatgtaagta cagatttaga
     5701 aggggctgga acagtttcta atcatcttat tgttcaacct tttaatgaaa ttcaggttgg
     5761 tttaggtcgt tatgatgaag atccagatga atatgagcat cctgatgatt cttcttatga
     5821 tactgttgaa tatgtttcag atgaaactac tgcacaaatt gaaatcaatg atattgaaga
     5881 atattagatc aaaatccagg ttctaatttt tgaaaaatta ttaataaaaa tgggagctta
     5941 agctcccatt tttattaata attatgttaa tttgaaaatt tctatacaat caacataaga
     6001 gtttaaatat gcagaaaaaa atagagtctc ttattttttc ctcataatag agttaaattt
     6061 tttaaaatac ttttgaaaga tagtattatt attcaagcca tattgattca attcactcgt
     6121 tatttcttga gctgtatttt tagattccct gataatatgt tcatagaatc ttatgaggac
     6181 atccaggagt tctaaagttc ttaactcatt gataacaact gtatcttcta aatagtgagc
     6241 gtctctatca aaaaatgtaa cgttagttaa agtagttggt attatatttg ccataggtgg
     6301 ataatttttc tgtgcccact gacaactatc actcagttgc cctgagtcgg ttttatgtaa
     6361 tgattcaacc tgattatgtt ttatctctat tacataaaca ctattatcta aataccaaca
     6421 tacatcagga ccttctttat attccttttc aggatttatt gaatcagcgc ctaagaagta
     6481 tcctaatagt ttgaagttat tttctacttt actactttta acgtcctcaa atccaaaatc
     6541 agacttaatc ttttgtagtt gtatcaaaat accttccaaa ttattgtaat cttttataat
     6601 tttttcaata tttattgcag agtttgttgg ttctttaaaa agctttcttg ggtaaactgt
     6661 taatggtcga atagctctag gatataattg tatagcccta ttttgaattt tcatactttc
     6721 caattcatca gtaaaattta caatacgagc aaagttttct aacattttcc ccttatactt
     6781 tgaatcagat aacaaattaa tagcattgtt aaatatttct gttgaaagat gaaattgatt
     6841 tatataggca tcctcataaa atttacgttc tgcataagcc aaatttagta atttttcatc
     6901 atatgaaaat tcagtttttt taaccttttt catttcagat tgataaccat tcttccaagc
     6961 tagatctcga ctcaaacagc tcgcgatagt ctttttaatg ttttctatcg gatctttttg
     7021 gtttttagca atttgtttac taactctaat acctaaatct aactgacaat ttgtttctgg
     7081 acttaatgcc gttctacagt ctttatgagc aataaatgaa cttaaatcac taccaactaa
     7141 gagtattact gaataatcaa catgagaacg tacagctctt cccatacctt gttctagtct
     7201 aaatatattt tttccaccga atccagttat attgcctaaa cattcagtat cataattatc
     7261 aactatactt tctccaaaag gtatttcatc tatcactaat attcgacaca agtcatctgc
     7321 aagatctaat ccttcaaacc tttgtgccat tacatagaaa cctgatttat ttatttgtga
     7381 acttaaatct gataaagctt cattaatatt ttcactattt aagaaaattg ccccttcatt
     7441 aacccaaact tctgcttgat ttatagatga tgtaagcaca aaaactgtcg tttgtttact
     7501 taaatcagca caaagaccaa tcactttatt tttatctaaa tctggcgaga taatagatgg
     7561 tacaaggatc atacgttcac ctgtaccttt atccgatgtg ggtgcaacca tattcttaac
     7621 aatatctttt tctagcccta aaaatctaat aaaattatct gaatcagaaa aagtcgcaga
     7681 cataaatagc ttatgcttag attcataata tggagttata cttttaatat ttaaaatatt
     7741 tggtgaaatt tcaaaatttg cctgactaaa tatacatcta caaaaattta gctcattttt
     7801 aataagagga taattaaact tcaaactttt atgatcttta tgtttattta ataacttaga
     7861 aatttcttct atttttgatt gccatatcca ataagggact tcaaaaggtt taccatcagt
     7921 ttcaaattca agccattgta tacctaaatg ttttttacat gatcccttta taatagataa
     7981 taattcagta taaatttctc ccgatgaaat aatagaaaat tgttttttaa tgatatccat
     8041 acctgtatga gcatcatcaa aaacaatggc agaaggttgg atcccatatg tcttaaaagt
     8101 actaaaccca ttaaatagct tttcatatgt tgagacaata atttcatcac cacacatagc
     8161 ttctggactt accgttctat gtccttttga gtaaagagtt gattgtatcc ctaactttgt
     8221 agcttctgct attacttgct cggctaattg taaagttgga accaaaaaca gaactggttc
     8281 attatatttt tgagcataat attttaagta gagtaaaccg attgttgttt ttccagaacc
     8341 agtactaagt ttaaggataa ctccttgttc gtctaattta gaatctaaaa tatcaataca
     8401 ctcttgttgt ataacccgca gtgagctatg agaacttttt gcatctaatg aattatacaa
     8461 ttcattaata ttattaattt taatagggtc attatctaaa aatatttcat cgaaaaaaga
     8521 cattttcact tccaatttgt tttgtaaaat tgatttatta tattgaaaat aaagtttttt
     8581 tagtgttttt aataaaatta aatagatact tttctgacca ctaccttcag attcatttaa
     8641 cataaaatct cttctacgaa atgcacgtgt tagaacccaa ttaaatcttt aattgggttc
     8701 acatcaaata ctctattgat cacgataagc tcgaactttc tcaaaaattg gataaaagtc
     8761 cagaccatca tccttcatat ctgaaattat gaccgcaatt actgcaatcg tagtgggcac
     8821 tttaccgttc tttttatagt tggtaatgga gttcgcgttc atatctaaaa gcgctgcaaa
     8881 ttctttaata gaaaggccag acttacctat aagtctttga aattcttcgt aaggcatagt
     8941 ttaaaaagca gaattgttct gatggaaaag tatagcacag atcatttatt tacatttaaa
     9001 cacataaata atgtgattaa cacactattt atgtgtttaa atgctcccat atccttcttt
     9061 caggacatat gaagaaggta cctttaatta tttatcctta aaatgaggca ttactatggg
     9121 taatttaaac ttgatcaatc atctatatct ttctgaaaac ggtagaaaaa ttggaacaca
     9181 gctaattaaa gatttctcaa ttaatagatc ttacaatctg gggttatttt taaacgtcaa
     9241 taaatgcttt gatgatcgag aagcaacgct ggtatggacg cagcattatc ttgatcaaca
     9301 tatagatgat gattacgaag acgttaaacg cgctttttta gcattctttc ctgatggagc
     9361 atttatgcaa ttttgagact gttaattgaa tagctagact ccctgaatgg tccttttaaa
     9421 ggaccattac acaatttaca gtcctacagt cagtttacct gacacagatt tttgtacgct
     9481 ctatcaaaat ccaaaacgag gaccatcaaa tcctttcgtt ttttcttgtg tctgtttctg
     9541 cttttcttgc tctagtaact tttgcttatt caattctacc tcatagtagg gtttgtacag
     9601 ctcataacga ttttcagctc tcaaccgatt aatttctaca atcatgtcct gtttttgctg
     9661 atagcgaact ggatcacttt ctttggcaaa ccgcagtgcc ttattcatca tcggcacatc
     9721 ttgcagatta gtatcgtatt ttttattgta atcctcattg gatagacaac tcacctcatt
     9781 cggaacctct acctttagcc ctctaatttc aagcactttg gtcggtgcat aacgctgtaa
     9841 gctgtcatta atgagttctt cagttttttg tcggtacaac tgcaattgtg ctttgcccat
     9901 ccctaataca tcggctgtta atttctgagc gccccctttc tctggctgtt cagggttata
     9961 acgcttaaaa aactgctcag gtgttcgctc tattccatcc acatggcgtt cactataaat
    10021 cagatgcaaa tggggttgtt cttgaccacc tagactacca acatgatgat ggatagcaca
    10081 gctataagga tattgataac ggtcagcaaa ctcttgaata aactgctctg ccaattcgat
    10141 acgttgttcc gatgtaagct cttttggtaa tgccaccacc aaacttgaac agactcgacc
    10201 atttttgcgt tcatacagat ccgaggcctg ccaaaattct tccggattcc cctcggcaaa
    10261 gctcggcata ttacctgagc aaacaaactc aagttgttca tgaacatgat ctttatgttg
    10321 tgcaaattgt gca
//
