LOCUS       NZ_CP055289            12103 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH7, complete sequence.
ACCESSION   NZ_CP055289
VERSION     NZ_CP055289.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 12103)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12103)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055289.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12103
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH7"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            join(11978..12103,1..666)
                     /locus_tag="HUK62_RS18695"
                     /old_locus_tag="HUK62_18695"
     CDS             join(11978..12103,1..666)
                     /locus_tag="HUK62_RS18695"
                     /old_locus_tag="HUK62_18695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_218015318.1"
                     /translation="MSLVDAISSPRLNTYKTSFNCNFDNDALKYYYWNQAISAEIYVLL
                     HNIEICLRNRIHTALSLNISELNNDTLSDNYKWYEFFDFNVPDKDDPTKMVLGETGKVV
                     ASAKRKLSQKRMRKTPQNVISNIDFGAWRHIFQISHCKNGEVIEWDKINPKIFVHYGDI
                     RNREKRKVVMDRLREIGLLRNRVAHLEPVWKFKERKIGNRVIAEPSSPTQIFSNLNQEI
                     AATVRFLGWLCTDTYSFYIKTKSYKNLQKLIQHQTIQDFGL"
                     /name="Abi family protein"
     gene            complement(944..1858)
                     /locus_tag="HUK62_RS18700"
                     /old_locus_tag="HUK62_18700"
     CDS             complement(944..1858)
                     /locus_tag="HUK62_RS18700"
                     /old_locus_tag="HUK62_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4504/CBY_0614 family protein"
                     /protein_id="WP_176538623.1"
                     /translation="MSAIELFSKRQKKLRGEVTDVYQYNEISNKLRVQVIQIIEDSFGD
                     KHEPSVQELYKEIHKIFCKEYGQFSLGNKYDSDSDALFNFFLNEKNYEHCLDIIEICFQ
                     AIHIIIRDSPDDYCSSQEPDSAIDELNIRFKEAGVGYQFEQGELIKVDSQFIHSEVVKP
                     ALQLLGKDKDYQGSSAEFLAAHEHYRHQRYKECLNECLKAFESVMKAIHKKHSWNFNKN
                     DNSKKLINNCLTNHLIPQYLQTQFCSISTLLETGAATIRNKEGGHGQGTEVKEVPEYLA
                     SYALHLTASNLLFLIKCEENYIK"
                     /name="STM4504/CBY_0614 family protein"
     gene            2461..2691
                     /gene="vapB"
                     /locus_tag="HUK62_RS18705"
                     /old_locus_tag="HUK62_18705"
     CDS             2461..2691
                     /gene="vapB"
                     /locus_tag="HUK62_RS18705"
                     /old_locus_tag="HUK62_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="WP_106440603.1"
                     /translation="MEIAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIDNP
                     WDVMLEAINEFEVGFQLERADQGEQFREEFR"
                     /name="vapB"
     gene            2688..3083
                     /locus_tag="HUK62_RS18710"
                     /old_locus_tag="HUK62_18710"
     CDS             2688..3083
                     /locus_tag="HUK62_RS18710"
                     /old_locus_tag="HUK62_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family toxin"
                     /protein_id="WP_012268403.1"
                     /translation="MIYLLDTNICIYVINNKPQHVFERFKQYQLGQLAISSITASELAF
                     GVEKSGSERNKQALNKFLSPLEILPYDEQAIWHYAKLRQDLQSTGKPIGSLDMLIAAHA
                     LALDVVLVTNNTKEFERIDGLKLENWV"
                     /name="type II toxin-antitoxin system VapC family toxin"
     gene            complement(3325..4026)
                     /locus_tag="HUK62_RS18715"
                     /old_locus_tag="HUK62_18715"
     CDS             complement(3325..4026)
                     /locus_tag="HUK62_RS18715"
                     /old_locus_tag="HUK62_18715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538624.1"
                     /translation="MKKISSTLILLLTTIASTSGFADNTNLVDQTKKNVKDLLKDPESA
                     QFRNIKVVINTEGRKSVCGQVNAKNSYGGYTGFQSFYAKSNDKIVYLNDDVNYQLAGCE
                     GKTNELKAKELQKEKLLKEKEEYVNKRVNNICHLQNQFIDDVIYNRKKIDIAYNRAKQW
                     FNLDSSLLKNFENEEYSSSQLKDDYLEALNKLQADPIKVKILRGNDYTARAKIMLDIKN
                     SCIEKYTLFFN"
                     /name="hypothetical protein"
     gene            4339..4959
                     /locus_tag="HUK62_RS18720"
                     /old_locus_tag="HUK62_18720"
     CDS             4339..4959
                     /locus_tag="HUK62_RS18720"
                     /old_locus_tag="HUK62_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538625.1"
                     /translation="MSQIAENEDTHYKVKIELFETNHNYLAAIMLNQAKMAKEEDIGHK
                     FRWIIPAMSFAVFNIESICNLYGSQLVKNWELLESSSFLGKVSLVSERLDLEVDWSKEP
                     WQTIKKMKDFRNALVHLKPKKTTAKYVYVKKTIDIELVPRYALLIDGYPSNNKSLMSYV
                     SIENMEKFIKAASDLERYWSWSCHHKKYDIHRYSTPITEIINS"
                     /name="hypothetical protein"
     gene            5241..5501
                     /locus_tag="HUK62_RS18725"
                     /old_locus_tag="HUK62_18725"
     CDS             5241..5501
                     /locus_tag="HUK62_RS18725"
                     /old_locus_tag="HUK62_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538626.1"
                     /translation="MKIMMTEDDQEREIAEAAARVLHDSFIEAAQTGPVFYVENDVLMS
                     KSPNQLPVVIKRLFRNLELSKRVPKQGTVKIRKKDIKCGLR"
                     /name="hypothetical protein"
     gene            5539..5874
                     /locus_tag="HUK62_RS18730"
                     /old_locus_tag="HUK62_18730"
     CDS             5539..5874
                     /locus_tag="HUK62_RS18730"
                     /old_locus_tag="HUK62_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650136.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_176538627.1"
                     /translation="MKKSSDHFVMFGVEELLQQVGQNIKTARIRRNITIKELANRSHVD
                     ERTISRLEKGDSSINFKNLVTVLMILGLEDSVYDLADPNQDQVGKALELQKQPKRVRKM
                     DQLSDDF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(5878..6435)
                     /locus_tag="HUK62_RS18735"
                     /old_locus_tag="HUK62_18735"
     CDS             complement(5878..6435)
                     /locus_tag="HUK62_RS18735"
                     /old_locus_tag="HUK62_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017398013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_176538628.1"
                     /translation="MKSLSVIELSSLYGMTRQAVYKQINKGNLSKNSDGKIDFSEAIRV
                     FGEPSKHVNSSQTTETRKLSEVHLLEQQVHMLQKQLEQAHEREKFQRDELKAKNDQLHV
                     KDEQIEAMQRLLEAPKTNMTTFTDQKPNQDIATDLRSETKPKHDGLTTPEPTENKRIPV
                     PEVMDVEPEKRGFWSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6512..7408)
                     /gene="repM"
                     /locus_tag="HUK62_RS18740"
                     /old_locus_tag="HUK62_18740"
     CDS             complement(6512..7408)
                     /gene="repM"
                     /locus_tag="HUK62_RS18740"
                     /old_locus_tag="HUK62_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749418.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_176538629.1"
                     /translation="MSLSKKELIVKSNQVIEASYQLSSTEQRIVLAAISKISRDAEITD
                     DEIYRVTVDDLKNLGVHEKTAYRDLKDGVNRLYERSINLAIDDESIKMRWVQSIRFLES
                     KSVVGLRFSKEILPFISNLSREFTKYSLSDIAGMSSAYAIRIYELLSQYRSIGKREISV
                     ENLRTMLELGKRYPLFADLKRWVIDTAVDQINEYSPLNVKYEQKKTGRKVTHIQFSFKE
                     KSKSINHHSEPEKFYKLTDAQINMFGNQLSRLHELSHLATQGESYDALAAKIKEMLRDP
                     IQQKQFTPHLRNLGFKG"
                     /name="repM"
     gene            9615..10601
                     /locus_tag="HUK62_RS18745"
                     /old_locus_tag="HUK62_18745"
     CDS             9615..10601
                     /locus_tag="HUK62_RS18745"
                     /old_locus_tag="HUK62_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538630.1"
                     /translation="MIHYSPNSRYTAQKIVDKVSNGAYFYSHFSVESDEMLLADKIQNI
                     IQKLTQKYALDLSPRQRTYRLNSKNEPIADLIVQKRFDLDTWDFWLLVTTPKSHEYNAL
                     KEDGELKKIAKQRVSEVETTWNREEERAQVALIQAYFQDHEKFKFVLQKPFLKLAFGQG
                     KFVELVRLSHSSKKSKTYAATEKSAKNYTWTWRYDEPTVQLLKKKYVGIMNTLISNKDK
                     SVGMNALKDFYDDLKRYSVFKGNRHQIGKLFVEAVSYHYKKTKHDFRKADYYVALELNY
                     LPRMQNYADHFTQYILMRRVHEFADVVLNKDAVHPDTYNDLLNEYLV"
                     /name="hypothetical protein"
     gene            complement(10613..11131)
                     /locus_tag="HUK62_RS18750"
                     /old_locus_tag="HUK62_18750"
     CDS             complement(10613..11131)
                     /locus_tag="HUK62_RS18750"
                     /old_locus_tag="HUK62_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538631.1"
                     /translation="MMKIKNMDSSKLSYMYFFPVVFFPFLNIYQFRNNPDLKSWLFSNL
                     LIGFTVILIPLFLALSMLITKFLYRDHNKKPEYNAMGLGLLCLIFLMGSNYYQFQKFTA
                     GTDLSIDLYRMALMLSFLIACFVSSLYFTLKYKQYSKKYTVDLNTKTQRFMASAALPLI
                     LTICALFTI"
                     /name="hypothetical protein"
     gene            11244..11537
                     /locus_tag="HUK62_RS18755"
                     /old_locus_tag="HUK62_18755"
     CDS             11244..11537
                     /locus_tag="HUK62_RS18755"
                     /old_locus_tag="HUK62_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538632.1"
                     /translation="MTEATFTFRVEESLKKEFSSFAKDMDRSGAQLLRDFMRDFVKKQQ
                     EAASYDEWFKKQVQIGLDEANAGKLIPHEEVKAEFAARRAATLAKLAAKNED"
                     /name="hypothetical protein"
     gene            11527..11796
                     /locus_tag="HUK62_RS18760"
                     /old_locus_tag="HUK62_18760"
     CDS             11527..11796
                     /locus_tag="HUK62_RS18760"
                     /old_locus_tag="HUK62_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825982.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_176538633.1"
                     /translation="MKIEWTETARQDRRNIYDYLDEHNPIAAIEIDDLIEEKTDLLVDH
                     VLMGRSGREKGTRELVLHPHYVVVYDVKDTVRILRVLHTSQQWG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
ORIGIN
        1 gtattacttc ataatattga aatctgttta cgtaatagaa tacatacagc tttaagctta
       61 aatatttctg aattaaataa tgatactctt tcagataact acaaatggta tgaatttttt
      121 gatttcaacg taccagataa agatgatcca acaaaaatgg ttctaggtga aacaggaaaa
      181 gttgtagcat ctgcgaaaag aaagttaagt caaaaaagaa tgagaaaaac acctcagaat
      241 gtgatatcga atattgattt tggtgcttgg cgacatattt ttcaaatttc tcattgtaaa
      301 aatggggaag taatagaatg ggataaaatt aatccaaaga tatttgtgca ttacggtgat
      361 attagaaata gagaaaagcg taaagttgta atggatcgct tacgtgaaat tggcttactg
      421 aggaacagag tggcgcattt agaacctgta tggaaattca aagaaaggaa aattggtaat
      481 agagtaattg cagaaccatc ttctccaaca caaatatttt ctaatctgaa tcaagaaatt
      541 gccgcaacgg ttagatttct gggttggttg tgtacggata cttatagttt ttatataaaa
      601 acaaaatctt ataaaaattt gcaaaaatta attcagcatc aaacaataca ggattttggt
      661 ctttaaaaag gaagagccac tcataagagt ggctctaaaa tctatgctga cagacaccac
      721 ttaatacgcg cttgggaatt aagcttgcat gaaaaaacat gcaggttctg aaaggctaca
      781 tatctagtat atatgaatag atgctaaagt aaagtttagt ttacagttta taaaaaagcc
      841 cgacatcctg ttgggctttt cgtattgggt gtttaggtat aacacttgtc ggatctcacg
      901 tcctgatgta gcgattaaac ctatagttaa gattctccat ctattattta atatagtttt
      961 cttcacattt aattaaaaac aataaattag atgctgttaa gtgaagtgca taactagcta
     1021 aatattctgg aacttctttt acttcagtac cttgcccatg tccaccttct ttatttcgaa
     1081 tggtagctgc tccagtttcg agtaatgtac ttattgagca aaattgggtt tgaaggtatt
     1141 gaggaattaa atgattagtt aggcaattat taataagctt tttagagtta tcatttttat
     1201 taaagttcca agaatgcttc ttatggatag ctttcatgac actttcaaat gcttttagac
     1261 attcattgag gcattcctta tatcgttgat gtctataatg ttcgtgagca gctagaaatt
     1321 cagcagatga accttgatag tctttatctt ttccaagaag ttgtaaggct ggtttgacta
     1381 cttcagaatg aataaattgt gaatcaactt taattagctc accttgttca aactgatacc
     1441 caacaccagc ttctttaaat cttatattta attcatcaat tgcactatct ggttcttgtg
     1501 aagagcaata gtcatctgga gaatccctaa taataatgtg tatagcctga aaacatattt
     1561 caataatatc taggcaatgt tcataatttt tttcgtttaa gaaaaaattg aaaagtgcat
     1621 cactatcact atcatattta tttccaagag aaaattgtcc atattctttg cagaatattt
     1681 tatggatttc cttataaagt tcttgaactg atggctcatg tttgtcgcca aaagaatctt
     1741 ctattatttg aatgacttgt acacgtagct tatttgaaat ttcgttatat tgataaacgt
     1801 cggttacttc tccccgtaat tttttttgtc tttttgaaaa cagttcaatt gcgctcaatt
     1861 tatatatctc gatttatgta atagctcata ttgtaatagc attttgttag aatttttaaa
     1921 attaacataa tggtacttat acgaaacaca ctttaaaata tcattgtttt tcatatagtt
     1981 gaaaaattaa ccaacctaaa aaaccgattt ataaataaat cggtttttta ttaagcaaag
     2041 ctgtaacttt tagccaataa gattgattaa aaacattctt ttttgcgttt tttcctgatt
     2101 tctcaagggt cacaattcag aaataatccc caatttctgc ggacaactct tgggataaaa
     2161 taggccaact ttgtaagcta aaacttacac agatttaagg attttgcgga ttggtaattc
     2221 aaggcattaa tcctatgatg actatatagg gaataggatg tttccagaaa aacaagaata
     2281 aaaaaagtag cgcaaggaat gggcagcacg aaatcagcaa gacgagcaac ccgatatgaa
     2341 aaagcccgac aggatgtcgg gcttttttat tgccttatga ttttaatttc actttgactc
     2401 tacaagattt aatgctatgg tatatatcaa tgatatacat cttggtttcg ggaggtaatt
     2461 atggaaattg cgaaagtatt tcaaactggt cgcagtcagg ctgttcgctt gcctaaagcc
     2521 tttcgcttta atggcacgga agtggccatc aaaagctttg ggcgtggtgt attactcatg
     2581 cccatcgaca atccttggga tgtcatgtta gaagccataa atgagtttga ggttggattc
     2641 caattggagc gagctgatca aggcgaacag ttccgtgagg agttcagatg atttacctct
     2701 tagacacaaa tatttgtatc tatgtcatca acaataagcc tcaacacgtt tttgagcgct
     2761 ttaagcaata tcaactcggc cagcttgcga tttcgagtat cacggcatct gagctggctt
     2821 tcggtgtaga gaagtctgga tctgaacgca ataaacaggc gttaaacaag tttttgtcac
     2881 ctttagagat tcttccttac gatgaacaag caatttggca ttacgccaaa ttacgccaag
     2941 atcttcaatc cacaggtaaa ccgattggca gcttagatat gctgatcgct gctcatgctt
     3001 tggcattgga tgttgtatta gtaacgaaca atacaaaaga atttgaacgg attgatggtc
     3061 tgaagctaga gaattgggtc taatcgctat atctagccct atgcagggct agaatttgca
     3121 taagagattt tatgttaaat ggccatttta actatgaaac taatttaatt ttatacaagc
     3181 tgagtcgtat caacttggac tataagttga gtattcaaat aaacatgcga aaaattcttt
     3241 tagaaaatta ggcttagctt atttatagat gaaattaaaa cagacataca catagttact
     3301 tttttatggc aacttaataa agcgttaatt aaaaaataat gtatattttt ctatacaaga
     3361 atttttaata tctaacataa tcttagcccg agctgtataa tcatttcctc ttaatatttt
     3421 aacttttatt ggatcagctt gaagtttgtt aagtgcttct aggtaatcat ctttaagttg
     3481 tgatgaggaa tattcttcat tttcaaaatt ttttaataga cttgagtcaa gattaaacca
     3541 ctgcttagcc ctattatagg caatatctat tttttttcta ttataaataa catcgtcgat
     3601 aaactggttt tgaagatgac aaatgttatt tactctttta tttacatatt cttctttttc
     3661 tttcagtaac ttttctttct gtaattcttt agctttaagc tcgttagtct ttccttcaca
     3721 ccctgctagt tggtaattta catcatcatt cagatataca attttatcat tactttttgc
     3781 atagaaactt tgaaacccag tataaccacc ataagagttt ttagcattaa cctgcccaca
     3841 tacacttttc cttccttctg tattgataac aactttgata ttacgaaatt gtgcagattc
     3901 gggatcttta agcagatctt ttacattttt ttttgtttga tccaccaaat tagtgttatc
     3961 agcaaaacca gaagtactag ctatagttgt taataataaa attaatgtag aagaaatttt
     4021 tttcattatg ttagataaca tgtgattaat aaaatcttga tgatatagta aattgctgag
     4081 gtagttcaag aattaaatca caatattcat tttactaaag taactaaatt ccgtgatttt
     4141 agataaagtg aagccttcag gagttttgat ttttttaata aaaaactgat gatgataaat
     4201 tgattttata taaataagtt gagtattaaa aataaacgtg cgaaaaattc ttttagaaaa
     4261 ttaggcttag cttgtttata tatgaaatta aaacagactt acacatggtt acttttttat
     4321 ggcaacttag gagtttttat gagccaaata gcagaaaatg aagatactca ctataaagtg
     4381 aaaatagagt tatttgaaac aaatcataac tatttagcag caattatgct aaatcaggcc
     4441 aaaatggcaa aagaagaaga tataggacat aaatttagat ggattattcc agcaatgtcg
     4501 tttgcagtat ttaatattga atcgatttgc aatttgtatg gaagtcaatt agttaaaaat
     4561 tgggaattac tggaaagttc tagtttttta ggaaaagttt ctttagtcag tgaaaggctt
     4621 gatttagagg tagattggtc taaagagcct tggcagacaa ttaagaagat gaaagatttc
     4681 aggaatgcct tagttcattt aaaacccaaa aagacaactg ctaaatatgt ttatgtaaaa
     4741 aaaactatag atatcgagtt agtccctcgt tatgcgttac ttatagatgg ttatccatct
     4801 aataataaaa gtcttatgtc gtatgtcagt attgaaaata tggaaaaatt tattaaagct
     4861 gcatcagatt tagaacggta ctggagttgg agctgtcatc acaagaaata tgatatacat
     4921 cgttattcaa ctcctattac tgaaattata aatagttaaa tatttgaaag atggatgctt
     4981 tttatttagt tttcctaaaa ttaacataat gcacgttata cgaaattgac ttgtttttgt
     5041 gatataaatc aactcgatag tcccctgacc tcggcccaat atctccacat tgggcttttt
     5101 tatccatttt tcatgttcca cgcctattct ttgatcaaag ttccacgcct ttgcttagct
     5161 agactgaaca atcacctaca ggtgttgagg ctcattttat ggtattaaac tggagttagt
     5221 catacgtccg tgagatagag atgaagatta tgatgaccga agatgatcaa gagcgtgaga
     5281 ttgccgaagc cgcagcgcgt gtgttgcacg atagctttat cgaagctgct caaactggac
     5341 cagtttttta tgttgaaaat gatgtcttga tgtcgaaatc accgaatcaa ctccctgtgg
     5401 taattaaacg tctgtttcgt aatttagagc tttctaaacg tgtacctaag caaggaacag
     5461 tgaaaatcag gaaaaaagac attaaatgtg gtttaaggta atttaaactg catttaatgt
     5521 catgtttttg agcttttcat gaaaaaatcc tccgatcatt tcgtgatgtt tggggttgaa
     5581 gaattactcc aacaagtcgg tcaaaatatt aaaaccgctc gaatccgcag gaatatcacg
     5641 ataaaagaac tggcaaaccg gagtcatgtt gatgagcgca ccatcagccg gttagaaaaa
     5701 ggtgatagca gtattaattt taagaatctg gtgaccgtgt taatgatctt agggcttgag
     5761 gactcggtgt acgatcttgc tgatccaaat caggatcaag taggcaaagc actagaatta
     5821 caaaaacagc ctaagcgtgt acggaaaatg gatcaactga gcgatgattt ctaagtatta
     5881 gccattgggc aggaaaaacc ggctccagaa gcctcttttc tcaggttcta cgtccataac
     5941 ttctggcaca ggaatgcgct tattctctgt tggctctgga gtagtcaatc catcatgttt
     6001 cggtttagtt tcggatcgaa gatccgttgc tatatcttgg ttaggtttct gatcggtaaa
     6061 cgtagtcata ttggtttttg gggcttctag tagacgctgc attgcctcaa tttgttcatc
     6121 cttaacgtgt aactgatcat tcttagcttt taattcatct cgttgaaact tctcacgctc
     6181 atgagcttgt tcaagctgtt tttgaagcat gtgaacctgc tgttcaagta agtgaacttc
     6241 tgacaacttt cgtgtttcgg ttgtctgtga actgttcaca tgtttagatg gttcaccaaa
     6301 gacacgaatg gcttcagaaa aatcaatttt accatcacta ttcttgctta aattgccttt
     6361 atttatttgt ttatagactg cttgacgagt cattccatag agacttgaaa gctcaataac
     6421 agatagtgat ttcatgttgt catttgggtt gtctattatg tgaaccaaga tacatcaaga
     6481 aattgtcaac cgctacatac acactcaagc tttatccttt gaaacctaaa tttctaagat
     6541 gaggagtaaa ttgcttttgc tgtattggat ctctcagcat ttctttgatt tttgcagcca
     6601 gagcatcata actttctcct tgagtggcta aatgagagag ttcatgtaga cgagaaagtt
     6661 gattgccaaa catattgatt tgagcatctg ttaatttata aaacttctct ggctcactgt
     6721 gatggttaat gcttttggat ttttctttaa atgaaaactg aatatgagtg actttacgac
     6781 cagttttctt ctgctcatac tttacgttta aagggctgta ttcattgatt tggtctacag
     6841 ccgtgtcaat gacccatctt tttaaatcag cgaacagagg gtatcttttg ccgagttcta
     6901 gcatggttcg taaattctcg acagagatct cacgttttcc tatggatcga tattggctta
     6961 atagctcgta aatacgtata gcgtacgcac tactcatacc agctatatcg gataaggaat
     7021 atttagtaaa ttcacgactt aaattagaaa tgaacggtaa aatttcttta gagaatctta
     7081 gaccaacaac acttttactt tctagaaatc gtatagattg aacccatcgc atttttattg
     7141 attcatcgtc aatagctagg tttatcgacc tctcgtataa acggttaaca ccatctttta
     7201 gatctcgata agctgttttt tcatgtacac ctaaattttt taaatcatca acggtaactc
     7261 gataaatttc atcatctgta atttcagcat ccctactaat tttactgata gctgctaaaa
     7321 ctatacgttg ttctgttgag cttaactggt atgaagcttc aattacttga tttgatttaa
     7381 cgattaactc ttttttagat aagctcatta gttatacttt ttaaataaat tatgagaact
     7441 ataatattct catagttggt agtcaatccc tcatagttaa gtagtcaatc cctcatagtt
     7501 aagtagtcaa tccctcatag ttggtagtca atccctcata gttaatcttt aaaacccttg
     7561 ctatataagg tattcagcta ccttaaaaga tttaaaagat ttaaaaataa ttaaaaaggc
     7621 atttgaaaaa ttgcccttgg ttttcgctac gctcaaactc tattgaatct cgctttcgct
     7681 cgattcgtcg gggcaatttt ttggtcaata ttcccgtgaa tttgagaaaa agcaaaagca
     7741 agatcaaaag caactcggaa ttcgcttcgc tcataaagtt tttttgctcg ctacgctcga
     7801 tctaggctca attttctaca tgtgatttac gcttatttag gctgtgaagc ctaaaaagct
     7861 gtttttcagg gagtctaggt gcgaattttg gtggttcgct cgtagacact cgctctataa
     7921 atgcctttta gcggcttaaa atggcgtttt ttagcccgat tacaaagcta cgtgatgaaa
     7981 tgatggtttt tgttatgaaa tggcttaaat cgcaaatcag agcgaaatag acactcacct
     8041 cgtttcgcta ctttgccgta accgagggag ctatatctca ctatcgtaga gttcacgttg
     8101 tttatcgaat agagatgagt aggcaaatac tcatgtcatc ctcacaatac gtcgtttttt
     8161 atataaaaaa tggaaaaaac tcacagtatg cctgctcaca tcaatttttc taaattgcaa
     8221 atattccctg agttagcaag aaaactatca aacagattgg tgattaacag gactgttgaa
     8281 atatcacatc atgttttttc attgaaattt gcttagtcct gatttagatc gggagaccaa
     8341 tcccgatcga tcagaacaca atggcgacca aaccattttt agctatgttt tcgctttttt
     8401 agataacaac ataaaaccga ctcaaacttt cggaaacaca tctacatcaa ggatgcagat
     8461 tttaagactc cctttaccta atcgcactgc caatgcaact agagttcgcc ttgtgtttta
     8521 gcagtcaaca acctgccacg atttcaccgt aaacggctta tacgccctaa tcatcgtctc
     8581 tccctctcca tgccttgccg taacaactca atgtctaaca gcatggagat tcccaagtta
     8641 tttctaacga tcttagcgat tagaacgcca atgccctttt gtctatcgat acttccggtt
     8701 tcccctcacc gcggttcctt ggaatcacga ggatatgatc taaagacaaa atctgagcgt
     8761 atggctctta atctacacgt gtctggggtt tatgcttccc catgtatact gtttaagcta
     8821 tccgttgacc cttcttgctt gatgtaatct tactcaaagg tgccgctgct tccagatttc
     8881 tcctcagcta gcctgtaaca ttgctgttac ctcaaacgcc atcagttgtg ccaatgagct
     8941 gcgcacccct tgtatttcta caagtgcatt tgccgcaaca cattgccgtt attaacgaat
     9001 cttaacgtcc tgtcggattc tcacggtaat cgttaaaaca gggtttcgat gcaagcatct
     9061 agctaggcta tatctaacct aacgaccact tccccgactg aaaaacagtc agtttatagg
     9121 ctcccgtgtc cttgtcgccc aaccccgttt gcacggattg gatcgaaaac gagtattaca
     9181 actcgctagc gcccatcaga tcgtaccgaa cgccccttcg ggagtagtac gctcaatcgg
     9241 tcagtcgggg atggctaatc cgcgaccgtg cgctatctaa aaaaacatgg ctttaagcag
     9301 gctgtcgcct gctcaaaatc gtaaaaacga aatggtcagg cagttggtca gactgctttc
     9361 agtcaatgaa tatcccgact ataaaccaaa caaaaagagg cgtaaattcc gtcacctagg
     9421 aaccacttaa ttgcacaatg acaaattgtg tcaattaagt ccgaccaatg ccgaaggcat
     9481 tggtaaacgt ggctttgcca ctacaagcct tataaaataa ggctttcatt gaaaataaat
     9541 attgcttgat gacaatgaag tttttagaat attgtttaat gacaatttta taaatattgc
     9601 ttggtgacaa taaaatgatt cattacagcc cgaatagcag atataccgcc caaaaaattg
     9661 tagataaggt atcgaatggt gcgtattttt atagtcactt ttctgttgaa agtgatgaga
     9721 tgctattggc agataagatt cagaatatta ttcaaaaatt aactcaaaaa tatgctttgg
     9781 atttaagtcc ccgacaaaga acttatcgtt taaacagcaa aaatgaacct attgcagatt
     9841 tgattgtgca aaagcgattt gatctggaca cttgggattt ctggttgctt gtgactactc
     9901 cgaaaagtca tgaatacaat gcgcttaaag aagatggaga gttaaagaaa attgcgaagc
     9961 agcgtgtctc ggaagttgaa acaacgtgga atcgtgaaga agaacgagcg caggttgctt
    10021 taattcaggc gtattttcaa gatcatgaga aattcaaatt tgtactgcaa aagccatttt
    10081 taaaactggc atttgggcaa ggaaaatttg tggaactggt gcgtttatct cactccagta
    10141 aaaagtcaaa aacctatgca gcgactgaaa aaagtgccaa aaattacaca tggacatggc
    10201 gatacgatga gccaaccgtt caattgctta agaagaaata tgttggaatc atgaataccc
    10261 tgatttctaa caaggataag agtgttggga tgaatgcgct gaaagacttc tatgatgatc
    10321 tcaagcgtta ttcggtcttc aagggcaatc gccatcagat cgggaagtta ttcgtggagg
    10381 cggtaagtta tcattataaa aaaaccaaac atgattttag gaaagccgac tattacgttg
    10441 cgcttgaact caattacctt ccccgaatgc aaaactatgc ggatcatttc actcagtata
    10501 ttttgatgcg gagagtacat gagtttgcag atgtggtttt gaataaggat gctgttcatc
    10561 cagataccta caatgatctt cttaacgagt atcttgtttg aggcacttac ccttagatcg
    10621 taaataaagc acagatggtt aagatcaaag gcagtgctgc actcgccata aacctttggg
    10681 tttttgtgtt taaatccact gtgtattttt ttgaatattg cttatatttc agggtgaagt
    10741 acaggcttga aacaaagcac gcgatgagga aactcagcat taacgccatg cgataaaggt
    10801 caatactgag gtccgtgcct gcggtaaatt tctggaattg gtagtagttg ctgcccatga
    10861 ggaaaattag gcataacagt cctagcccca tggcgttgta ttccggtttt ttgttgtgat
    10921 ctcggtataa aaacttagtg atgagcatgc ttaaagcaag gaaaagtggg atcagaatga
    10981 ccgtgaagcc gattaataaa ttactgaata gccatgattt aaggtctgga ttgtttcgaa
    11041 attggtaaat attcagaaat gggaaaaaca ccacgggaaa gaagtacata taggacagct
    11101 tagacgagtc catattttta attttcatca tgagggatca gggtccgagc tgtcgttagg
    11161 ctgattctag cggttgtttt ggcatgtcat gttttctatt gtgttataat cgcattcaaa
    11221 tgtaacacat tggagttaaa gccatgacag aagcgacttt cacattccgc gtagaggaat
    11281 ctttgaagaa agaattttcg agttttgcga aggatatgga ccgtagcgga gcgcaattac
    11341 tgcgtgactt catgcgtgat tttgtgaaga agcaacaaga agctgcatct tatgatgagt
    11401 ggtttaagaa gcaggtccag atcggcttag atgaagccaa tgctggtaag ttaattccac
    11461 atgaggaagt aaaggcagag tttgctgcta gacgtgctgc aacattggca aaactggctg
    11521 caaagaatga agattgaatg gacggaaaca gctcgtcagg atcgtagaaa tatctatgat
    11581 tatcttgatg agcataaccc tatagcagct attgaaattg atgatttaat tgaagaaaaa
    11641 actgatttac ttgttgatca tgtgttgatg gggcgaagcg gtcgagagaa aggcacacga
    11701 gagttagtgc ttcatccgca ctatgttgtg gtgtatgacg ttaaagacac ggttcgtatt
    11761 ttaagagttt tgcatacatc gcagcagtgg gggtaactga catagacatc aaaatatcaa
    11821 actgtctgct aataaaatag caaatatata tattctaaaa gagggtcttt ttatttggga
    11881 cttacaactg aatttcaggt aaacataagt ttagttgatg aagtttatac ctttaaaatt
    11941 attgtaatat aaggaaattt tttattaaaa acttactatg tctttagtag atgctatcag
    12001 ttcccctaga ttaaatacat ataaaacaag ctttaactgt aatttcgata atgatgcgct
    12061 taagtattac tattggaatc aggctatctc agcagaaata tat
//
