LOCUS       NZ_CP055288            13036 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH6, complete sequence.
ACCESSION   NZ_CP055288
VERSION     NZ_CP055288.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13036)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13036)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055288.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13036
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH6"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            join(12418..13036,1..98)
                     /locus_tag="HUK62_RS18615"
                     /old_locus_tag="HUK62_18615"
     CDS             join(12418..13036,1..98)
                     /locus_tag="HUK62_RS18615"
                     /old_locus_tag="HUK62_18615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538622.1"
                     /translation="MKKLAITLLVSTALTSVFAAPKSSTSALYPLDSFNPQKFTASNIK
                     EFLTKYSSEADVIVKKGEFETTAEYQARLAKGIQTKSLDSDKIYAFKLDSISVTYDPDE
                     AQYNVRSTEGNLFGRRLILGTNYETTILISNLSPKGYVNKNFYIESSKPFYNVNSGNLT
                     FSTDIEVAKKNASCKKQVYVFAKLNGQVYQNSPYNAPINRFDPDYTIPMDVVGMVLKCS
                     TGTVLSVYESKFQQSS"
                     /name="hypothetical protein"
     gene            70..330
                     /locus_tag="HUK62_RS18620"
                     /old_locus_tag="HUK62_18620"
     CDS             70..330
                     /locus_tag="HUK62_RS18620"
                     /old_locus_tag="HUK62_18620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538613.1"
                     /translation="MNQNFNKALEEICQRLIDVIDNSLDSEEDKNEVKNVIYELKEANT
                     KSSIRSAYNNLTTLMSNHITIGTAILTSNILPFINQLFMLN"
                     /name="hypothetical protein"
     gene            complement(375..965)
                     /locus_tag="HUK62_RS18625"
                     /old_locus_tag="HUK62_18625"
     CDS             complement(375..965)
                     /locus_tag="HUK62_RS18625"
                     /old_locus_tag="HUK62_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538614.1"
                     /translation="MYSRRSNLVLGFHGLDESIGKRIIAGEDELLPSENDYDWLGHGIY
                     FWENSLARAQMWAEQQSQRKDTSVKKPFAIGATIDLGNCLDLLDQYWLDYVGDAYTFMV
                     EELEAEGKEIPKNTAWTTTDIDFKKRELDCAVILYAIQMAEKEGEPFDSVRAAFWEGED
                     LYPNAGFKQHSHIQISIINPQCIRGIFLPKQDI"
                     /name="hypothetical protein"
     gene            complement(970..1152)
                     /locus_tag="HUK62_RS18630"
                     /old_locus_tag="HUK62_18630"
     CDS             complement(970..1152)
                     /locus_tag="HUK62_RS18630"
                     /old_locus_tag="HUK62_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538615.1"
                     /translation="MQTQALSLREKARRSFEARKSDHQARLAFLKDAKILDASGNYNDQ
                     FFSKSSTNTDQARAK"
                     /name="hypothetical protein"
     gene            complement(1305..1916)
                     /locus_tag="HUK62_RS18635"
                     /old_locus_tag="HUK62_18635"
     CDS             complement(1305..1916)
                     /locus_tag="HUK62_RS18635"
                     /old_locus_tag="HUK62_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_104794708.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLTRSGYKPTGKKA
                     NEAKHKRIKELLAVGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            2372..3001
                     /locus_tag="HUK62_RS18640"
                     /old_locus_tag="HUK62_18640"
     CDS             2372..3001
                     /locus_tag="HUK62_RS18640"
                     /old_locus_tag="HUK62_18640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538616.1"
                     /translation="MNKLILTTCVFSILLSGCKNPFEAKDKGIDQLNTIENRWEDTQIL
                     ASSTARIALATPISELQEIRRDLKKSEVSECLTPAKEALISYMDSRISNFLNFMSETES
                     TYFEINPKIIEYFSIKNKCTGEQSDPNSILVKEAKEAEEYEAKINAEMKEQGFDIKEKG
                     TPAYKAARVAAEAAIAVKEARIAVAEASIAAEEVAAEAEARAELLY"
                     /name="hypothetical protein"
     gene            complement(3245..3469)
                     /locus_tag="HUK62_RS18645"
                     /old_locus_tag="HUK62_18645"
     CDS             complement(3245..3469)
                     /locus_tag="HUK62_RS18645"
                     /old_locus_tag="HUK62_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_176538617.1"
                     /translation="MPYEEFQRLMGKAGLSIKEFATLLDMNPNSITNYKKIGKVPTHIA
                     VLVYLLSNMKDEGVDFYPIFEKIKSYSKD"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3620..3865
                     /locus_tag="HUK62_RS18650"
                     /old_locus_tag="HUK62_18650"
     CDS             3620..3865
                     /locus_tag="HUK62_RS18650"
                     /old_locus_tag="HUK62_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            4408..5169
                     /locus_tag="HUK62_RS18655"
                     /old_locus_tag="HUK62_18655"
     CDS             4408..5169
                     /locus_tag="HUK62_RS18655"
                     /old_locus_tag="HUK62_18655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538618.1"
                     /translation="MQNSESKKRGRPALLLNQLELKAYVDYVRKYKIWDQVTQNAVREI
                     QASQFEMQNLSPTALVIVKQAIKPFRELQKVELLYQQLCKTTSHDFFQKKFVELYQQYQ
                     STREDQTLNVLKTMATQYLRFKDNKVDENSLKLYLSQLEKKENKAKRNADNKKKFELGG
                     ALLALLKTKNIDVTKLTAAQMIRALFSQDMHFNLANCNTIIFQEMLNVGLSDTKAKNLF
                     PLVLDQLTDYRDEDGLPIYLKQIIRTMAENG"
                     /name="hypothetical protein"
     gene            5162..6214
                     /locus_tag="HUK62_RS18660"
                     /old_locus_tag="HUK62_18660"
     CDS             5162..6214
                     /locus_tag="HUK62_RS18660"
                     /old_locus_tag="HUK62_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_176538619.1"
                     /translation="MASYHLQLRDKPKGYALEHYFYIARLEKYQHVRVKSEEVLEHIEP
                     GRCMPIWAKDPADFWKAADTHERANAKVYMEYELALPNELTPEQRKTLVEAFLNEHVAS
                     HQYPHSYAIHNVKSRIGGIDQPHCHLMLSLKANDGLDRTPETYFKRFNPKNPEKGGAKK
                     RQLDEGFDNYRSFLLYIRKEWENYMNDALRQYAPTLTYQIDGQEINIPNRVSSSSYKQY
                     NLDHGTFYIAEPKLGVGNQNNTPEYLEQIKQIREHNQQEFEREQLQKHQELNLSAKPFY
                     SVNDQPYSSLEVTALLKSLNPDTHSVEITAIQQQQALVDPSFKNQELNLTPENKPTPTI
                     KKDTGFNFSI"
                     /name="MobA/MobL family protein"
     gene            6750..7724
                     /locus_tag="HUK62_RS18665"
                     /old_locus_tag="HUK62_18665"
     CDS             6750..7724
                     /locus_tag="HUK62_RS18665"
                     /old_locus_tag="HUK62_18665"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_164543322.1"
                     /translation="MLKNNLIVKSNQVIEASYTLTTIEQRLILSAIAQVPKGEEISDDV
                     LYPLTTKNLVKLGGDGKASHKEFKDAVNRLYERSIVLRDGDESDSFRWIQEKVFKESTV
                     AFIRFSKPILPFLSNLKAEFTKYLESDIVGMSSPYSIRFYELIMQYRSVGHREISLENL
                     RWMFQLQNKYPVWADLKKRVIEQAIKEINEHSPYNLTIEPKRTGRKITSIVLKFQDKKS
                     LKTPKESTNNPLFETQRNRDFVAPNIQIFSHALKNPEKAKESDLGHRASRITGLIMSNG
                     LADRFKQGNESIIGMMKRIKEEITTHTTANQWENKLKEFGVVF"
                     /name="replication initiation protein"
     gene            7807..8358
                     /locus_tag="HUK62_RS18670"
                     /old_locus_tag="HUK62_18670"
     CDS             7807..8358
                     /locus_tag="HUK62_RS18670"
                     /old_locus_tag="HUK62_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_164543323.1"
                     /translation="MPKFTVAELAKKYGIARTSIYDRMKNGSISYEIDAKNRKVIDFSE
                     MQRVYGTPDSKAYSQPDSTQTDNTTIELYKQLIEELRHDKSVAEERERRMYKEIDQLKD
                     KIDSLTLSLGYTPKEIQPDSQPDSRNEQTRTTTDLQEIVHIEQVQPIENKPIQQTSKIQ
                     PEPKKRGLFGRVLQAVLSDD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(8743..9875)
                     /locus_tag="HUK62_RS18675"
                     /old_locus_tag="HUK62_18675"
     CDS             complement(join(8743..9623,9623..9875))
                     /locus_tag="HUK62_RS18675"
                     /old_locus_tag="HUK62_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032072965.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_223235881.1"
                     /translation="MARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQNQIIDW
                     KNQLISASSQAFDQSKAPTEPPIDLKKLHAKIGEQALEIGFFRRCVEETGPLQPQKLID
                     DSLQISVSKQAQLLKVSRGCYYYRPKPVSASDLKLMRCMDELHMQYPFAGSRMMRDLLN
                     RQGHHIGRRHTRTLMKKMGINALYCKPNLSQANQAHRKYPYLLKGLAIQRSNQVWSTDI
                     TYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVTFCIETLNEAIEKYGRPEIFNTDQG
                     SQFTSDAFIDVLKSNGIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSNVLDAKKQ
                     LNAYFEFYNLKRPHSSLDKMTPDEFYYDQLPQQNKVA"
                     /name="IS3 family transposase"
     gene            10276..11064
                     /locus_tag="HUK62_RS18680"
                     /old_locus_tag="HUK62_18680"
     CDS             10276..11064
                     /locus_tag="HUK62_RS18680"
                     /old_locus_tag="HUK62_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="WP_267314107.1"
                     /translation="MAQKYHPDRYKGPDANDKMVKIREAYEVLSNPEKRKAYDNYLKEI
                     KEKNKHENTNQKTTQSYAHTHTYTSPKKPNFNFIKKETVKKYTKYFFYTLLSITLIGII
                     LALVIPAYDNYIVKNQTIEKMAGLRLGLSQEEVKLLKGKPTIIDKIEDHDGFYTLYWYY
                     GYPSGKYLAITFSGTDKDNLVTSMICGHPDDMPIEGIWHANSVYENVFYETFGKPEYID
                     VSKDRLSKWISYPKLNAAFEMKDNSAISVCLTKDYVEYVK"
                     /name="DnaJ domain-containing protein"
     gene            11581..11901
                     /locus_tag="HUK62_RS18685"
                     /old_locus_tag="HUK62_18685"
     CDS             11581..11901
                     /locus_tag="HUK62_RS18685"
                     /old_locus_tag="HUK62_18685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538620.1"
                     /translation="MKKYLIILLSSLLFGCSNSDSEPIVEVISEKDKLAFKRCAGVVKS
                     YGSHPEKFEYYPNESTNFTNQEGREVVTLSFAFGNASHEKIYHKAECVIYPNGQATMTQ
                     MM"
                     /name="hypothetical protein"
     gene            11933..12157
                     /locus_tag="HUK62_RS18690"
                     /old_locus_tag="HUK62_18690"
     CDS             11933..12157
                     /locus_tag="HUK62_RS18690"
                     /old_locus_tag="HUK62_18690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538621.1"
                     /translation="MQKNLDAYDKDMNLIGEFDGEFLYNINGGSLSDYRVDGDEIYTRN
                     PNEIQKHIGTFENGKAILFTGELLFQCTD"
                     /name="hypothetical protein"
ORIGIN
        1 attacacaat tcccatggat gtagtaggta tggtattgaa atgctcgaca gggactgtct
       61 tatctgtcta tgaatcaaaa tttcaacaaa gctcttgagg aaatttgtca aagactgatt
      121 gatgtaatag ataactcttt agatagcgaa gaagacaaaa atgaagtaaa gaatgtaatc
      181 tatgagttaa aagaagctaa tactaaatca agtattagaa gtgcttataa taacctaact
      241 acattaatgt caaatcacat aactattggg actgcaattt taactagcaa tattttacca
      301 tttattaatc aattatttat gttaaattaa atattataaa catataaatt ttccacaaga
      361 tatttgaaaa attttcaaat atcttgttta ggaagaaaga tgccacggat acattgagga
      421 tttataatcg aaatttgtat atgactgtgc tgtttaaacc ctgcattagg atatagatct
      481 tctccctccc aaaatgcagc tcttacagaa tcaaatggtt ctccctcttt ttcagccatc
      541 tgaatggcat ataaaatcac agcacaatcc aattctcttt ttttaaagtc gatatcggta
      601 gttgtccaag cagtattttt aggtatttct ttaccttcgg cttctaactc ttcgaccata
      661 aatgtatatg catcacccac ataatctaac cagtattgat cgagaagatc gaggcaattt
      721 cctagatcta tagttgcacc gatagcaaaa ggcttcttta cgcttgtatc ttttctttgg
      781 ctttgttgct cagcccacat ttgagcacga gcaagactat tctcccaaaa atagatgccg
      841 tgacctaacc aatcatagtc attttcacta ggaagtaatt catcttcacc agcaattatt
      901 cgcttaccta ttgattcatc taaaccatga aaaccaagaa ctaaattaga acgacgacta
      961 tacatttaat tatttagctc tagcctgatc agtattagta gatgatttag aaaagaattg
     1021 gtcattataa ttaccacttg catcaagaat tttggcatct ttcaaaaatg ctaatctagc
     1081 ctgatgatca ctcttacggg cttcaaaaga acgacgagcc ttttctctta agcttagggc
     1141 ttgagtttgc ataatgttct cctatttcat gtttagtata gcaaaaatag aaatgcccct
     1201 agattaaaaa ttgtatagct ataatctatt taacataaaa tctcttatgt gaagcaacct
     1261 gaatgcagcg gagccccatt ggggcgcgtt gagataagcc tcttttagat aactttagca
     1321 actcgataaa cagttgcaac tccacaattc actgctttag caatttcttc cttagtcata
     1381 ttgccaactg ctagcaattc ttttatccgt ttgtgcttag cctcatttgc cttcttgcct
     1441 gttggtttgt aacccgaacg tgtcaggccc tgcttaatac gttctatacg tttctcattg
     1501 tccagacgag ccatcgttgc cagaagatca atcaacatat tattgatgag ttccaggatt
     1561 gaatgagtaa tgctgtcact ggtttgaatc atttggtagg tagtaggaag atctgctacg
     1621 actaaacgaa gtcccttctc ctgaatcctg cgcttcagct cttgaaaatc tcgttgggtt
     1681 aggcgtgata agcggtcaat actttcaacc aacaaggtgt caccttgatt tgcttctgac
     1741 agtagcttat tcaattcagg tcggtctaac ttcgtaccac tatagttttc cacgtacaca
     1801 atggtttcgc ccaaattcaa attttggttt agatcctgaa gaatctgcaa agccctttct
     1861 gcatcttgat ctttagttga agctcgtaga taaatacgtg tattcatttc tatcatttag
     1921 ccttaattcg attagatcaa tgataatact atcatttaat tattgcatta agtttaatga
     1981 tagatgcttt atattatttg gctcgctatc attcagctat agtgcaataa taggttactt
     2041 aaatcggcac taacctatag aattaatata aatattaaaa tgttgctgag ggtagtatga
     2101 gttctaaaag tggttttgcc ttagaggatc tgttagcgat cactggttgg ttagttctta
     2161 tcataggtgt attaggtggt ttttttcagt ttggagtaac ctttttcgct ctaatcacag
     2221 ctatatcatc attcttctta gcgttaacaa tgttcacgtt ctctgaaatt ctaacgtgcc
     2281 taaaaaaaca atagtctaat gataatattg atggtttatt aatattagag gattgcatag
     2341 ggcgattttc ttaaaaaggt ttttgcattt aatgaataaa ttaatattaa caacatgtgt
     2401 tttctcaata ttgcttagcg gttgcaaaaa tccatttgaa gctaaggata aaggtataga
     2461 ccaattaaat actattgaaa atcgttggga agatactcaa atattagcct catctacagc
     2521 acgtatagca ttagctacac caattagtga attacaagaa ataagaagag atttaaaaaa
     2581 gtcagaagtc agtgagtgtt taactcctgc aaaagaggcc ttaatctctt acatggattc
     2641 acgtatttct aattttctta actttatgag cgagactgaa tcgacttatt ttgagatcaa
     2701 tccaaagata attgaatact tttcgattaa aaataaatgt acaggtgaac agagcgatcc
     2761 aaattcaata ctagttaaag aggctaaaga agcagaagaa tacgaagcta aaattaatgc
     2821 agagatgaaa gaacagggat tcgatataaa agaaaaagga acccctgcat ataaagctgc
     2881 aagagtagca gcagaagcag caatagcagt aaaagaagca agaatagcag tagcagaagc
     2941 atcaatagca gcagaagaag tagcagcaga agcagaagca agagcagaac tactctacta
     3001 atttagtttt attcacatac atcggcctgt ttatcttctg tagtacatga tttaccgaat
     3061 gctcgaccta agatctcaga aaataagaat ttgatgatca ccctggttct caaaactcga
     3121 tactttattg aaaagttcta tcgaggctta taagttcctg aaacaaaaaa atgcctgaag
     3181 ttcaggcatt ttttacatca tcaatatcta ctaaaaagct tacaacaatc aaaaaacgag
     3241 agttttagtc ttttgaatac gacttaattt tttcaaaaat agggtaaaag tctacaccct
     3301 catctttcat gttagaaagt aagtaaacca gaacagcgat atgagtaggt acttttccga
     3361 tctttttata gttagttatg gagtttgggt tcatgtccaa taaggttgca aattccttaa
     3421 ttgaaagacc tgctttcccc ataagtcttt gaaattcttc gtatggcatc gtgataaata
     3481 caatctgttt gaattagaag tataacacag accataaaac ttaaaaaaaa cacataaaaa
     3541 atgtgacaaa cacatttttt atgtgttata aatagcttat caactaacct atttatcctt
     3601 actatcggag aaatgtaata tgtactcata cccggattca aacactgaaa agaaaattgc
     3661 tctcatgatc atcaatgact ttttcattca aaaagcccat gaactgtgga tttttttaca
     3721 actcgatcag tgctttaatg attatgaagc cacactgatt tggactcgac gttatttaga
     3781 gaagcatcct gagtgtgagt acagcgatat tcaaaaagcc tttgtttctt gtttcccaga
     3841 gcacttcttc agctttgact gttaatttat gagaggcacg gtaaaaaatc gcaattggta
     3901 agtaaatccc aaatagaaag agccagttca tgggcttttt attgtcacgt ataaatctaa
     3961 gacttgtttt aatcgttgct aacacgatca agcaaagtgt aataacgaaa ctaaaagtgc
     4021 agcaacagac tttttttttg cagaaataaa actgaaaatg cggtaacaga aacgtttttg
     4081 tagaaacgaa actgaaaatg cagcaacaga catctttttt gccggaaaca agcgcaattt
     4141 aagcgaaaag cacagcattg ttttgatttt aaaatttaag catattgctt aaatatcagc
     4201 agcttaggtt caatacctaa gctgctgatg cccaggggtt gaggggaagg attccctcac
     4261 ggactgtagt ttctcgaaga gaaactgtta agtgcgaatt aataaaaaat aaaattaatt
     4321 ttatttttta ttaattaaaa tccattcgaa aaatgaatta tgctacaacg cataatgaaa
     4381 cttttcgtga atggatttct tgttaagatg caaaactctg agtcaaaaaa acgtggacga
     4441 ccagctcttt tactcaatca actagagtta aaggcctatg tcgactatgt gagaaagtat
     4501 aaaatttggg atcaagtcac tcaaaatgca gttcgtgaaa ttcaagcatc tcaatttgaa
     4561 atgcagaact tgtcaccaac tgcattagtc atcgtaaaac aagccattaa accatttaga
     4621 gagcttcaaa aagttgaact gctttatcaa caactttgta aaactacatc gcatgatttc
     4681 tttcaaaaaa agttcgtcga gttatatcaa caataccaaa gcactcgtga agatcagaca
     4741 ctgaatgtgc ttaaaacaat ggcaactcaa tatcttcgtt ttaaagacaa caaagtcgat
     4801 gaaaatagct taaaactgta tcttagtcag ttagagaaaa aagagaataa agcaaaacgg
     4861 aatgctgata acaagaaaaa atttgaatta ggaggagcac tattagcctt gttaaaaaca
     4921 aaaaatattg atgtcaccaa actgaccgca gcacaaatga ttcgtgctct ttttagtcaa
     4981 gatatgcact ttaatttagc caattgcaat acgatcattt ttcaagaaat gctcaatgtt
     5041 ggcctcagtg acactaaagc caagaactta tttccactgg ttttagatca actcacagac
     5101 tatcgcgacg aagatggttt gccgatttac ttaaagcaga ttattaggac aatggcagaa
     5161 aatggctagc tatcacctgc aactaagaga caaacccaaa ggctatgcac tcgaacatta
     5221 tttctatatt gctcgattag aaaaatatca gcatgtgcga gtcaaaagcg aagaagtctt
     5281 agaacatatc gaacctggcc gttgcatgcc aatctgggcg aaagatcctg ctgacttctg
     5341 gaaagcagca gatacccatg agcgagccaa tgccaaagtc tacatggaat atgaattggc
     5401 tctccccaat gaacttacac cagaacagcg taaaacactg gtagaggcat ttttaaatga
     5461 acacgtagcc agtcaccaat atccgcattc ttacgccata cacaacgtta aatcgagaat
     5521 tggaggcatt gatcaaccgc attgtcatct catgttgtct ttaaaagcca atgatggcct
     5581 tgaccgcacg ccagaaacct acttcaaacg ctttaatccg aaaaacccgg aaaaaggcgg
     5641 ggcgaaaaaa agacaacttg atgaaggctt tgacaactac cgttcattct tactgtatat
     5701 ccgcaaggag tgggaaaact atatgaatga tgccttacgc caatatgccc cgaccttgac
     5761 ttaccaaatc gatggacaag agatcaacat ccccaatcga gtatcgtctt caagttataa
     5821 acaatataat ttagatcacg gcacctttta cattgccgaa cccaagcttg gagttggcaa
     5881 tcaaaacaat acacctgaat atttagagca gatcaaacaa atccgcgagc ataaccaaca
     5941 agaatttgaa cgcgaacaac tccagaaaca ccaggaactc aacctcagtg ctaaaccgtt
     6001 ttacagtgtg aatgaccagc cgtatagcag tttagaagta acagccttat tgaagagttt
     6061 aaatccagat acccactcag tagaaataac cgcgattcaa cagcaacaag ctttagttga
     6121 tccatccttt aagaatcaag aactcaatct cactcctgaa aataaaccaa caccgactat
     6181 taaaaaagac actggcttta acttttcgat atgacacgga atcaaccgag cgagtgtcta
     6241 cgagcgctat caccaaaatt cgcgcctaga cttcttgaaa aacagctttt taggctgtga
     6301 agcctaaata agcgtaaatc acatgtaaaa aattgagcct agaccgagcg tagcgagtaa
     6361 aaaggcttta tgagcgaagc gaattccgag ttgcttttga tcttgctttt gcttttcctc
     6421 aaattcacgg aaacattaac ctaaaaattg ccccgacgaa tcgagcgata gcgagattca
     6481 atagagtttg agcgtagcga aaaccaaggg caatttttaa atttactctt ttttattatt
     6541 ttaatacttt taaaagattt taagccttct gaaaccctta tatagcaagc ttcttcagct
     6601 tttagtatgg acagattccc gctatacatg gacagattcc cgctatacat ggacagattc
     6661 ccgcatcaat atggacagat tcccgcactg ttgacatcaa agatggacaa atatatattt
     6721 gtccataata atttaaagtt actatcaata tgcttaaaaa taatttgatc gtaaaatcaa
     6781 atcaggtgat tgaggcgtct tatactctta caacaataga acaacgatta attctaagtg
     6841 ccattgctca ggtgccaaaa ggtgaggaaa tatcagacga tgttttatac cctttgacta
     6901 ccaaaaattt ggtcaaactc ggtggagatg gaaaagcttc acacaaagag tttaaggatg
     6961 ccgtaaaccg tttatatgag cgatctatcg tacttcggga tggtgatgag tctgactcat
     7021 ttagatggat tcaagagaaa gtatttaagg agtctacagt agcttttatt cgcttctcaa
     7081 agcccatatt gccattttta tctaatctga aagctgagtt cactaaatac ttagaatccg
     7141 acattgttgg tatgtctagt ccttactcta ttcgtttcta tgaactaata atgcaatacc
     7201 gtagcgtcgg ccatcgtgaa ataagccttg aaaatcttcg ttggatgttc cagctacaaa
     7261 ataaatatcc tgtttgggct gacttaaaaa aacgagttat tgagcaggca atcaaagaaa
     7321 ttaatgagca tagcccttac aaccttacta ttgagccaaa acggacagga cgcaaaatca
     7381 caagtattgt tcttaaattt caagataaaa aatcattaaa aacaccaaaa gagtcaacaa
     7441 ataatccgct ttttgaaact cagagaaata gggattttgt cgcccctaac atccaaatct
     7501 ttagccatgc tctgaaaaat cctgaaaaag ctaaagaaag cgatttaggg catagagcaa
     7561 gtaggattac agggttaatc atgtcgaatg gtttagcaga tcgatttaag caaggtaatg
     7621 aatcaattat aggcatgatg aaacgtatca aagaagaaat tacaactcat acaacagcaa
     7681 atcaatggga aaacaaattg aaagaatttg gcgtggtttt ttagtcatga ccaaataatc
     7741 tcaagacaca attatttgca ttggtagaaa tactttgaga tctacaacaa tttgaagagt
     7801 ttaataatgc ctaaatttac tgttgctgaa ttagctaaaa aatatgggat cgctcgtaca
     7861 tctatttatg atcgtatgaa gaatggttct atcagttatg aaattgatgc taagaatcga
     7921 aaagtcattg attttagtga aatgcaacgt gtttacggta cacctgacag taaggcttac
     7981 agtcagcctg acagtactca aactgacaac actacaatag aactgtacaa gcagttaatt
     8041 gaagaattac gacacgataa gtcagttgct gaagaacgtg aaagacgtat gtacaaagag
     8101 attgaccaac ttaaagataa gattgacagc ctcacacttt cactcggata taccccaaaa
     8161 gagattcagc ctgacagtca gcctgacagt cggaatgaac aaacccgtac aaccacagac
     8221 ttacaagaaa tcgtacatat tgaacaagtt caacctatcg aaaataaacc cattcaacaa
     8281 acgtctaaga tacaaccaga acctaaaaag cgaggcttat ttggtcgagt tttacaggct
     8341 gttttgagtg atgattaaag ggctattgtt gaaagtgact aaacaaactt gtggaacatt
     8401 gttcagatat taggcgtgaa acatgagaaa tcataaaaaa agcccaagta cgcatacttg
     8461 ggctttttac tatgcgataa gactatgatt tatataaaat aaaaagaagc atttcgtata
     8521 aggtgtatta tgttaattgt aggaaatcta caatataaat tattataaaa actcccttat
     8581 tatttgacag aatcacaatc tattttatta aaatttatag agtttttatg aaaaacagtt
     8641 ttaggtgtgt ctttattaac aactattttt tgtacaaatg cagaggtggt cccacatgtt
     8701 tgaacaacta aaagcttatt tataagtgat actctgctct agttaagcta ccttattttg
     8761 ttgtggtagc tggtcatagt aaaactcatc tggagtcatt ttgtccagac tcgaatgagg
     8821 tcgtttcaaa ttataaaatt caaaatatgc gtttaattgc ttcttcgcat ccaaaacatt
     8881 gctgtaggct ttgagataca cctcttcata tttaacgctc cgccataatc gttcaaccat
     8941 cacattatca acccatcgac ctttaccatc catactgatt tgaatgccat ttgatttcaa
     9001 tacatcaata aatgcatcac tggtaaactg actgccttgg tctgtattaa atatttcagg
     9061 tcgaccatat ttttcaatag cttcatttaa tgtttctatg caaaatgtaa cctccatact
     9121 aatcgatact ctatgcgcaa gtaccttgcg gctatgccaa tcaatcacag cacataaata
     9181 aacaaagcct tttgccatag ggatatacgt tatatccgta gaccacactt gattactgcg
     9241 ctgaatagcc aatcctttga gcagatatgg atatttgcgg tgagcttgat tagcctggct
     9301 taaatttggt ttgcaatata acgcattaat gcccattttc ttcattaaag tacgtgtatg
     9361 acgtcgtcct atatgatgtc cttgacgatt caacaaatca cgcatcatcc ggctacctgc
     9421 aaaagggtat tgcatatgta actcatccat acaccgcatt agcttcagat ctgatgcact
     9481 cacaggtttt gggcgatagt aataacaacc acgggagact ttcagcagct gagcttgctt
     9541 agatactgaa atctgaagtg agtcatcgat taacttttgt ggttgaagcg gcccagtttc
     9601 ttcaacacac cttctaaaaa atcaatttct aatgcctgct caccgatttt tgcatgtagt
     9661 ttttttagat cgatgggtgg ttctgttgga gcttttgatt gatcgaaagc ttgcgaggaa
     9721 gctgaaatca gttgattttt ccagtcgata atttggtttt gatgaacatc aaattcagca
     9781 ctcaattcag caagtgtttt ttctgcttta atcgcagcaa gtgctacctt agctttaaaa
     9841 tcatttgaat gatttcttct tggtctacgt gccataaaat actccatata ttgatgttta
     9901 taacatcatt tggggagcaa aatatcactt ataagtgttg ttcaaatttc ctgatccacc
     9961 tcttgctttc tcaggcattg aggtaataaa ccatagtgtt cctcaaaaca gaagaagcta
    10021 aatctttaaa tttttttctt ttcccgataa gaaaattgta ttttttactg ccaaattaaa
    10081 ataagcgtta atagaagcac gtactaatca atttatttat aaaaatcagt aattttctta
    10141 tgttaataat ataattcaat acaatagtta aagttagatg catgaacata tgagttttta
    10201 taaaatatta catcctagaa ccacatcata tcagaagatg ctcctgatga aatcattaag
    10261 ttagcatata aaggattggc tcaaaaatat catcctgatc gctacaaagg tcctgatgct
    10321 aatgacaaaa tggtaaaaat tagagaggca tatgaagtct taagcaatcc tgaaaaaaga
    10381 aaagcttacg ataattattt gaaggaaata aaagaaaaaa ataaacatga aaatacaaat
    10441 caaaaaacta ctcaatcgta tgctcataca cacacttata cttcacccaa aaaacctaat
    10501 tttaatttta taaaaaaaga aactgttaaa aagtacacaa aatatttttt ttatacatta
    10561 ctaagtatta cccttatagg gataatactt gctttagtaa ttcctgccta tgacaattat
    10621 attgtaaaaa atcaaaccat tgaaaaaatg gcagggttac gtttaggatt aagccaagaa
    10681 gaagtaaaat tattaaaagg gaaaccaacg ataattgata agattgaaga tcatgatggt
    10741 ttttatacct tatattggta ctacgggtat ccaagtggga aatatttagc tattacattt
    10801 tcaggaactg ataaagataa tttagtcaca tctatgatct gtggacaccc tgatgacatg
    10861 cctatagaag gtatttggca tgcaaatagt gtatatgaaa acgtttttta tgaaactttt
    10921 ggaaaacccg aatacatcga tgtctctaaa gatagattat ctaaatggat ttcatatcct
    10981 aaactaaatg ctgcttttga aatgaaagat aattcagcaa tatcagtatg tctaactaaa
    11041 gactatgtgg aatatgttaa ataaaaacca aatttattgg ctgtaattta aaattaaaaa
    11101 gcctagcttt agcatctagg ctttttatga tttgtatcaa agcttataac tatgtaagcg
    11161 tccgctgaac cccacttttt ctaactgaac cgtaccgggt ttgtcggaga cttttttatt
    11221 taagaaagtg ctagcaattt taagaaagaa aaagcctgat aggtcaaggg ccctatgata
    11281 tttgtcgtgt acagaggtaa ttgtcaaaag atattgaatg taaacttttc tttgatccta
    11341 aatatcccat gacaatacct atagaaacta tagctgaata cttaatttat tatcacacct
    11401 aaaaccatag gaaatgaggt tttacagagg tttccatgtg ctagtttttt tagaatttag
    11461 ctatagttct gatccacccc tataattaaa tttaataaaa ttatttttgg tattcttttt
    11521 atagtgtaat gatagttttt ttcatctaag ataatatttt attaaaatgc atgactttaa
    11581 atgaaaaaat acttaattat attgctctca tctcttctgt tcggctgtag caatagtgat
    11641 tctgaaccta ttgtagaagt tattagtgaa aaagataaat tagcgtttaa acgatgtgct
    11701 ggggttgtaa aaagctatgg atctcatcct gaaaagtttg aatattatcc aaatgagtct
    11761 actaacttta caaatcaaga agggcgtgaa gtcgtcactc ttagctttgc tttcggtaat
    11821 gctagtcatg aaaaaatata tcacaaagca gaatgtgtta tatatccaaa cgggcaagca
    11881 actatgaccc aaatgatgta attaatttaa aaaaaactag gaacttcaaa aaatgcaaaa
    11941 aaatctagat gcttatgata aagatatgaa tctaattgga gagtttgacg gagagttttt
    12001 atataacatt aacggaggtt cactatcgga ctatagggtc gatggcgatg aaatatatac
    12061 aagaaatcct aatgaaatac agaaacacat cggtactttc gaaaatggca aagcaattct
    12121 tttcacagga gaattactgt ttcaatgtac tgactgaacc gtaatgggtt tgtcggaggc
    12181 tttttattta agttaggcca cctaacctaa cagattaatt tcatctatag ccatattcca
    12241 atcatctagt tgactatcat gcaataacca aatcgattga gattcagcta attgacgtgc
    12301 taatctcgta taatcactat acgagaaagc gagctaaatc attaacataa agttaaatat
    12361 gtaattggtt taggataatg tattattgac acaactaatt gataaaaatt taattttatg
    12421 aaaaagctag ctataacgct attagtctca actgctttaa catctgtatt tgctgctcct
    12481 aaaagtagta cttcagcact ctacccattg gatagtttca accctcagaa attcaccgca
    12541 tctaacatca aagaatttct gacgaagtat tcttcagaag cagacgtaat tgtaaaaaaa
    12601 ggggagttcg agactacagc agaatatcaa gctagattag caaaaggaat acaaactaaa
    12661 tcattagact cagacaaaat ttatgcattc aaactggatt caatctctgt cacatatgat
    12721 cctgatgagg cgcaatataa tgtaagaagt actgaaggaa acttatttgg tcgacgactt
    12781 attttgggta caaattatga aactacgatc ctgataagca acttgtcacc aaagggctac
    12841 gttaataaaa atttttacat tgaatcatca aaacctttct acaatgtaaa ttctggtaat
    12901 ttaacattct caactgatat tgaagtcgcc aagaagaacg ctagctgcaa aaaacaagtg
    12961 tatgtatttg caaaactcaa tggacaggtt tatcaaaaca gcccatataa tgctccaatc
    13021 aacaggtttg atccag
//
