LOCUS       NZ_CP055287            13799 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH5, complete sequence.
ACCESSION   NZ_CP055287
VERSION     NZ_CP055287.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13799)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055287.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH5"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            complement(join(13790..13799,1..239))
                     /locus_tag="HUK62_RS18525"
                     /old_locus_tag="HUK62_18525"
     CDS             complement(join(13790..13799,1..239))
                     /locus_tag="HUK62_RS18525"
                     /old_locus_tag="HUK62_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016165574.1"
                     /translation="MSKNDKQTSKDVSSLASDVLRDPSSSAIQRQLAGSALSQANSDKQ
                     TGSKMETKASNVLQSDKYSDTTKTLAASVLAQSNKER"
                     /name="hypothetical protein"
     gene            583..720
                     /locus_tag="HUK62_RS18530"
                     /old_locus_tag="HUK62_18530"
     CDS             583..720
                     /locus_tag="HUK62_RS18530"
                     /old_locus_tag="HUK62_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016165573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016165573.1"
                     /translation="MSSTPLKPGPKPIADSTGEVDKRRRDNKKTPGNNDELKPSKSTNK
                     "
                     /name="hypothetical protein"
     gene            complement(837..1847)
                     /locus_tag="HUK62_RS18535"
                     /old_locus_tag="HUK62_18535"
     CDS             complement(837..1847)
                     /locus_tag="HUK62_RS18535"
                     /old_locus_tag="HUK62_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_069119648.1"
                     /translation="MATTVTGAFNTFQKDIVNLDSSTTINARSSRNWLLEQIAKFPDKN
                     SNFPILYPDINIAFGSFARKTKTRPLDDIDLMVGISADGCYYSENSWDDISLCFPDNYT
                     GKLKNYADTRNSSYLSSTRITNAFKTELGNISQYSSAEIKRDGEAATLKLVSYSWNFDI
                     VPCFFTKPDIFGNTYYLIPNRNGGWKKTDPRLDRDRLSAVNQNQAGNVLNVIRILKYWK
                     QEKRVNIGSYLLEAMVLYYYESKSNNTCSKYVDLELENVFNYLANSILNTVADPKKIQN
                     NINDFDYYDRYSLSEKFKKFAQLSNEARQLEIDQKIERSINKWKEVFGDNFPDYG"
                     /name="hypothetical protein"
     gene            complement(1988..2917)
                     /locus_tag="HUK62_RS18540"
                     /old_locus_tag="HUK62_18540"
     CDS             complement(1988..2917)
                     /locus_tag="HUK62_RS18540"
                     /old_locus_tag="HUK62_18540"
                     /inference="COORDINATES: protein motif:HMM:NF037206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-4TM family putative pore-forming effector"
                     /protein_id="WP_069119653.1"
                     /translation="MTISQRQNEPESIKKLQAQRQMYSDIKFWMIFISIVGVVLPIIVS
                     FITFAMNNDFFSKLLNFEKKDIGYISAFIGISTAIYVEIHSNFLKKLKEDAAKIQEKFD
                     TDVFQLPWDNINVGSKPDTGLIFKKSKKFDKKNPNYTGFSNWYTLKAASFRYPESIVFC
                     QQQNLYWDSSLRKDIVFLSKITLVAIIVMICALGFYNDFSLRNLVTNVVLLLLPICLFF
                     CKIITEHNETIKEMERLRDINESLIETIVTNTSTSTDFLSQCRQLQTAIYNHRKSARPI
                     PNWLHKKRKDDQEEESDDRMQQYLDTHS"
                     /name="S-4TM family putative pore-forming effector"
     gene            complement(2975..3919)
                     /locus_tag="HUK62_RS18545"
                     /old_locus_tag="HUK62_18545"
     CDS             complement(2975..3919)
                     /locus_tag="HUK62_RS18545"
                     /old_locus_tag="HUK62_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000287 - magnesium ion binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="WP_176538610.1"
                     /translation="MNNQDTKLQQAYDILVQLGMPRQQLNERTALCLLCLLDMTPKKAW
                     DQASNPLVGITPIMNWAREHYQREYAPNTRETFRRQSMHQFIEAGICLYNPDKPDRPVN
                     SPKAVYQIEFNLLQVLQTYGTEQYETMLQNYLQQRQTLAQMYAREREMNMIPLQLTDGQ
                     TILLSAGDHSQLIKDIVTDFGARYVPGGVLVYAGDTGNKHGFFDVDLLASLGVQLDNHG
                     KLPDVVIYNPEKNWLFLIEAVTSHGPVDHKRYGELNTLFQSCTAGLVFVSAFPDTKTYV
                     KYSGVIAWETEVWMADNPSHMIHFNGSRFMGPY"
                     /name="BsuBI/PstI family type II restriction endonuclease"
     gene            complement(3916..5346)
                     /locus_tag="HUK62_RS18550"
                     /old_locus_tag="HUK62_18550"
     CDS             complement(3916..5346)
                     /locus_tag="HUK62_RS18550"
                     /old_locus_tag="HUK62_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397137.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="WP_176538611.1"
                     /translation="MNLMTVEHIRKTTNAKLNPKKKSELGQFMTPSVIAEYMASLFDDH
                     KKEIKLIDCGAGIGSLTISAAKKLKNINLVDLWEIDPIMQEQLEINMHAMKLNFSIYAQ
                     DFILGAVENISSDKGERYTHAIINPPYKKINSNSEHRRELRKVGIETVNLYSAFVALTI
                     QLMEQDGQIVAIIPRSFCNGPYYKPFREFILKECAIEHIHIFESRDMAFKDDDVLQENI
                     IIKLTKGKQQSDVQISQSNDQTFRDYQSKTVPFVEVIKPNDPELFIRIATNEQPQEENN
                     TFFAVSLNELGISVSTGPIVSHRMKEFLEQSPQDGAVPLFYPHHFVDKQLQYPKEHKKP
                     NAIRVTPDSQKWLLPNNGFYVIVRRFSSKEEKRRIVANVIDPNMIDKKWIGFDNCWNVF
                     HVKKQGFDYETAMGLACFLNSSLLDSYFRIFSGHTQVNATDLRNMKYPSLQNLQLLGKK
                     YDIKMNQKQIDNLLGEIT"
                     /name="N-6 DNA methylase"
     gene            complement(5624..5938)
                     /locus_tag="HUK62_RS18555"
                     /old_locus_tag="HUK62_18555"
     CDS             complement(5624..5938)
                     /locus_tag="HUK62_RS18555"
                     /old_locus_tag="HUK62_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005098515.1"
                     /translation="MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(5925..6212)
                     /locus_tag="HUK62_RS18560"
                     /old_locus_tag="HUK62_18560"
     CDS             complement(5925..6212)
                     /locus_tag="HUK62_RS18560"
                     /old_locus_tag="HUK62_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_005244631.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFDDPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDRYEHG"
                     /name="BrnT family toxin"
     gene            complement(6481..6738)
                     /locus_tag="HUK62_RS18565"
                     /old_locus_tag="HUK62_18565"
     CDS             complement(6481..6738)
                     /locus_tag="HUK62_RS18565"
                     /old_locus_tag="HUK62_18565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_120385287.1"
                     /translation="MKKSITTVSIILLTLVLSACDSKEKKAFIRGCAEGLADKSICSCA
                     YDKLNDDATKMYGKEWGTNPAILQDERFFYALQKAAMQCE"
                     /name="hypothetical protein"
     gene            complement(6744..6962)
                     /locus_tag="HUK62_RS18570"
                     /old_locus_tag="HUK62_18570"
     CDS             complement(6744..6962)
                     /locus_tag="HUK62_RS18570"
                     /old_locus_tag="HUK62_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004906505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_120385288.1"
                     /translation="MKISHIVIVGYLVLAFFTAIYGNFWGDYDYKGFAYNLGRGLIWPA
                     VWFPAFGKFLGGLFIIAFVAYLTLSKR"
                     /name="hypothetical protein"
     gene            complement(7251..7541)
                     /locus_tag="HUK62_RS18575"
                     /old_locus_tag="HUK62_18575"
     CDS             complement(7251..7541)
                     /locus_tag="HUK62_RS18575"
                     /old_locus_tag="HUK62_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_127219988.1"
                     /translation="MRTIKYTSAFKKDYKREKKGQHRNTVDTELQTVLELLQADEPLPE
                     KYRDHALVSNWVGHRDCHIKPDLVLIYKKTEEGELLILKLVRLGSHSELVI"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(7528..7788)
                     /locus_tag="HUK62_RS18580"
                     /old_locus_tag="HUK62_18580"
     CDS             complement(7528..7788)
                     /locus_tag="HUK62_RS18580"
                     /old_locus_tag="HUK62_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_005254175.1"
                     /translation="MASNALVTTRINENIKHEATEVLATMGLTVSDAVRLLLTKVAKEK
                     ALPFEIWQPNAETIAAMEEDLSKAKSFTSVKDLMADLNADD"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            7907..8332
                     /locus_tag="HUK62_RS18585"
                     /old_locus_tag="HUK62_18585"
     CDS             7907..8332
                     /locus_tag="HUK62_RS18585"
                     /old_locus_tag="HUK62_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180106.1"
                     /GO_process="GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="WP_228723782.1"
                     /translation="MYIQNKLDNIIQELKELSGNSRLNIELPDDISISSYEQKIGFIFP
                     KDYKKVLKEVSNIFYGTIELASLTDEKEYYRGLSQILNDAREQGLPEDWLPICEDNGSY
                     YCLSPNHKIRYWTADGYSDEQWEDLADWIKQVWIDGN"
                     /name="SMI1/KNR4 family protein"
     gene            complement(8521..9087)
                     /locus_tag="HUK62_RS18590"
                     /old_locus_tag="HUK62_18590"
     CDS             complement(8521..9087)
                     /locus_tag="HUK62_RS18590"
                     /old_locus_tag="HUK62_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_032024465.1"
                     /translation="MNTATKYSVSDAATLYKKSRATIYKDIKNGVLSRDYDGFIDFSEL
                     LRVYGEPFGAKQAKRIDTSSIQNQNTPEYTDKTVVELKNQIDFLKKQLEKAEDRESKAN
                     ARIDTLLTLIEMKPPTANMTTFTDQEPLQPSMAKTAPEPDTAEAKQDGLTTPEQTENKR
                     IPVPEQVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(9151..10074)
                     /gene="repM"
                     /locus_tag="HUK62_RS18595"
                     /old_locus_tag="HUK62_18595"
     CDS             complement(9151..10074)
                     /gene="repM"
                     /locus_tag="HUK62_RS18595"
                     /old_locus_tag="HUK62_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004775003.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_032066855.1"
                     /translation="MTNNNKLVVKDNALIDASFNLSLIEQRIMLLAIVGARESSNLTSD
                     TPIEVFVSDYVHQFKVDSNNAYGLLREAAKTLKRREFSYLDRYKGQEALTTANWVNKVT
                     YVDSSGLIVLYLSNEVISLISRLSEQFTKYHIEQVSDFKSKYSIRLYELLIKWLNVAKT
                     EKYSINDLRSKLGLDATEYSTMSNFKSNVLDRAISEINKHTNITVDYDQFKKGRVITDI
                     QFRIKSKDKPSQQELTKVSQTAFHQMTDAQINMFGNQLSRLPEFSNLANGNESYESLAA
                     KIKEMLRDPIQQKQFLPHLKNLGFKA"
                     /name="repM"
     gene            11250..11636
                     /locus_tag="HUK62_RS18600"
                     /old_locus_tag="HUK62_18600"
     CDS             11250..11636
                     /locus_tag="HUK62_RS18600"
                     /old_locus_tag="HUK62_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032001883.1"
                     /translation="MNSTRTVAVRLDNQVAQEYERLAQLKGDKLGTYLRDLLTSNLQMA
                     SIERQANRIETLVNDFEKQINYSLDKFTEANYLNEKFFEDFGHIYMFQIGILMHLKVDR
                     GDIQGMQTKGNAYAQKNYFAGKKP"
                     /name="hypothetical protein"
     gene            11633..11830
                     /locus_tag="HUK62_RS19205"
     CDS             11633..11830
                     /locus_tag="HUK62_RS19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228723783.1"
                     /translation="MSNIIQLSKPCAFCDSRENVQIFSGLMLCGNCQDNIRITNPEPYR
                     VCRRPLFLRECPDEKSKIHP"
                     /name="hypothetical protein"
     gene            11805..13024
                     /locus_tag="HUK62_RS18605"
                     /old_locus_tag="HUK62_18605"
     CDS             join(11805..12081,12081..13024)
                     /locus_tag="HUK62_RS18605"
                     /old_locus_tag="HUK62_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611467.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_100833221.1"
                     /translation="MKKVKYTPEIRDRAVQLLIESEKDYPSTWAAITAIAPKIGCTPET
                     LRVWYQKYLDKQNPIKVQQLSDQERIKQLERENKELQRANEILRKAAGFFRPGGARPPT
                     QIMVDFIQNNKDRYGVEAICRILPIAASTYYRALDLVDNPEHRAKRALHDLHHAEQIKR
                     IWKESLGRYGVRKVWQQLKREGYVIARCTVARLMQKLGIQGVWRGKNKQTTRSRDDQKR
                     ADDLVKRNFNADHPDQLWVADFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMV
                     LDALEQALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAET
                     VNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFEFEAMYYDKI
                     NPLGQVA"
                     /name="IS3 family transposase"
     misc_feature    12080..12196
                     /locus_tag="HUK62_RS18605"
                     /old_locus_tag="HUK62_18605"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(13113..13679)
                     /locus_tag="HUK62_RS18610"
                     /old_locus_tag="HUK62_18610"
     CDS             complement(13113..13679)
                     /locus_tag="HUK62_RS18610"
                     /old_locus_tag="HUK62_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016165575.1"
                     /translation="MNEEKDPEIKYLSNDEDLFHVEKKIYLPTFIFQEFFVFLCITFGS
                     YFSIFISSQKFATEESFSKSFNLVFAFFTIENLIDTSIGLFIVMGILCFVNHLTTHSKL
                     NIQPILNRLISAVTDFYYLMFSTILGCMLGLFHFLHKFPMIKEYEQLHQLAISGMITIG
                     LVGSTTSIAIQFFVHKNKVLHKKPD"
                     /name="hypothetical protein"
     Typing          complement(9152..10074)
                     /name="rep_cluster_1283"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.333"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          12123..13021
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.667"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgttggact gggcgagtac agatgctgct agagttttcg tggtatcgct atatttatca
       61 ctctgtagga cattggatgc ttttgtttcc attttactac ctgtttgttt atcagagttc
      121 gcttgggata aggctgaacc tgctagctgt ctttgaattg ccgaagatga tggatcacgt
      181 aatacatcag atgctaatga actaacatct tttgaagttt gtttgtcatt tttagacata
      241 attatttcca tgaaattaaa agtaatctca tgatcataat taatattttg ttgtgatcaa
      301 tatttgttcc gcaggaaagc gtcttaagtt gtcgtatatc gtatcgtgtt tagagtcgaa
      361 ctgaataaag agacttatgt ttagtagatg atcaaaaggt catgaacgtg atagaacttt
      421 tcatgtaacg tgtattattt aaattttaga agacttgaat aaaaaaatat cggataaggt
      481 attgaaaaaa ataaaaaagt catcaagtta tattttgacg tagggtaaat tctgcgttta
      541 cgaacaaatt actaagtaaa caaaatttta acggagacta atatgtcgag caccccactt
      601 aaaccaggac caaaaccaat tgctgattca acaggtgagg ttgataaaag acgacgcgat
      661 aataaaaaaa cgccaggcaa taatgatgaa ttaaaaccta gtaaatcaac gaataaatag
      721 tatgaaaagt ccgacattct gtcgggcttt ttttctgttc gtataagaga ttttatgtta
      781 aatgaacgct aaaaatgccc taattacagg catttttagc tcaaatttga ttaatcctag
      841 ccataatctg ggaaattatc gccaaaaact tctttccatt tatttataga cctttctatt
      901 ttctggtcaa tctcaagctg tctagcctca ttcgaaagtt gagcaaattt tttaaatttt
      961 tcactcaatg agtatctatc atagtagtca aaatcattga tattattttg aatttttttt
     1021 ggatcagcca cagtattgag aatactattt gccaagtagt taaaaacatt ttctaattct
     1081 aaatcaacat actttgaaca tgtattattt gatttacttt catagtagta caggaccata
     1141 gcttctaata aatacgatcc gatgtttact cttttttctt gtttccagta cttcaatatg
     1201 cgaattacat tcaaaacatt accagcctga ttttgattga cggctgataa cctatctcga
     1261 tcaagtctag gatcagtttt tttccagcct ccatttctat taggaattaa ataataagta
     1321 ttcccaaata tatctggctt cgtgaaaaaa catggaacaa tatcgaaatt ccatgaatag
     1381 cttactaatt ttagagtagc agcttcccca tctcttttta tctctgctga ggaatactgg
     1441 ctaatgtttc ctaattcagt tttgaaagca ttagtaattc ttgtagagct taaataagat
     1501 gaatttctag tatctgcata gttctttaac ttacctgtat aattgtcagg aaaacagagg
     1561 cttatatcat cccatgaatt ttcagagtag tagcatccat cagcgctaat tccaaccatt
     1621 aaatcaatat catcaagagg tcttgttttt gtctttcttg caaatgaacc aaaagcaatg
     1681 tttatatcgg gatataaaat aggaaaattt gaatttttat caggaaattt agcaatttgt
     1741 tctaatagcc aatttcgact agatctagca ttaattgtcg tacttgaatc taaattaaca
     1801 atatcttttt gaaaggtgtt gaaagcacct gtaactgttg ttgccatatt tatcctaatt
     1861 ctcaagttca tcctataaat taggtttaat tcttagattt aaagtactaa ttttaattaa
     1921 ataaaaaaat ctttatcaaa ttttgataaa gattttttat agtggtcaag ctgttttaaa
     1981 tatcgtatta ggagtgagta tcaaggtatt gctgcattct atcgtcagac tcttcttctt
     2041 gatcatcctt tcttttttta tgtaaccaat tcggtatagg acgtgcagat tttctatgat
     2101 tataaattgc tgtttgtagc tgacgacatt gagacaagaa atctgtggaa gttgaggtgt
     2161 ttgtgacaat cgtttcgatt agactctcat tgatatctcg caatcgttcc atttctttta
     2221 tagtttcatt atgctctgta ataattttgc aaaaaaacaa acaaataggt aataaaagga
     2281 gcacaacatt ggtaacaaga ttccttaatg aaaaatcatt gtaaaatcct agagcacata
     2341 tcattactat aattgcgact aatgttattt tagataaaaa aacaatatcc tttcttaaag
     2401 aactatccca ataaagattt tgttgttggc aaaatacgat tgattctggg tatctaaatg
     2461 aagcagcttt caaggtatac cagttagaga aacccgtata attcggattt tttttatcaa
     2521 attttttact tttcttaaaa attaatccag tgtcaggttt gcttccaaca ttaatattat
     2581 cccatggcaa ttggaaaaca tctgtatcga atttctcttg tatttttgca gcatcttctt
     2641 tcagtttttt taaaaaatta ctatgtattt caacataaat tgctgtgctt atcccgatga
     2701 aagcggatat atatccaata tcctttttct cgaaattaag taactttgag aaaaagtcat
     2761 tattcatagc aaaagtgatg aatgaaacaa taatgggtag aactacgcct acgatagata
     2821 taaaaatcat ccaaaatttt atatctgagt acatttgtcg ttgagcttgt agttttttaa
     2881 tgctttctgg ctcattttgt cgttgagata tggtcatctt tcttactcat gaatgtctgt
     2941 ttaactttga aaatgttatt caatcgagaa tagattagta aggacccata aatcttgagc
     3001 cgttgaagtg gatcatgtgg gacggattat ccgccatcca gacttcagtt tcccatgcaa
     3061 taacaccgct atatttcaca taggtcttag tatcaggaaa agcagagaca aaaactaaac
     3121 ctgctgtaca gctttggaac aatgtattta attcaccata tcttttatgg tctacaggtc
     3181 catgactggt aacagcttca attaaaaata accaattttt ctcaggattg tagataacaa
     3241 catctggtag tttaccgtgg ttgtctaatt gcacacccaa agatgctagt agatcaacat
     3301 caaagaaccc atgtttgtta cctgtatcgc ctgcataaac taatactcct ccaggaacat
     3361 atcgagcacc aaaatcggtg acaatatcct tgatgagctg agaatggtca cctgctgaaa
     3421 gtagaatagt ctgaccatct gttagttgta atgggatcat attcatttcg cgctcgcgcg
     3481 catacatttg agcaagagtt tgtctctgct gaaggtagtt ctgtagcatg gtttcatatt
     3541 gctctgttcc ataagtttga agaacttgaa gcaaattgaa ttcaatttgg tatacagctt
     3601 tagggctatt aacgggtcta tcaggcttat cagggttata aagacaaatt cctgcttcaa
     3661 taaactggtg catcgattgt cgacggaatg tctctcgtgt atttggagcg tattctcttt
     3721 gataatgctc gcgtgcccag ttcatgatag gcgtaatacc tactaatggg ttacttgcct
     3781 gatcccatgc ttttttaggt gtcatatcaa gtaaacagag tagacagaga gcagtacgtt
     3841 cattaagttg ttggcgcggc atgcctaatt ggacaaggat atcgtatgct tgctgcagct
     3901 ttgtatcttg attattcacg taatctcccc gagaaggtta tcaatctgtt tctgattcat
     3961 tttaatgtcg tatttcttgc ctaaaagctg caaattctga agggatggat atttcatgtt
     4021 tctgagatcc gttgcattta cttgggtatg acctgagaaa atacggaaat agctgtctaa
     4081 taagctacta ttcagaaaac aagccaatcc cattgctgtt tcatagtcaa agccctgttt
     4141 ttttacatga aatacattcc aacagttatc aaacccgatc cattttttat caatcatatt
     4201 gggatctatt acattagcga caattcgtcg tttttcttct ttagaactga atcgtctgac
     4261 aatcacgtaa aagccattat taggtagcag ccatttctgt gaatctggtg taaccctaat
     4321 agcatttggt ttcttatgct ctttcggata ttgaagctgc ttgtctacga aatgatgtgg
     4381 gtaaaataat ggaacagctc catcttgagg cgattgctcc aagaactctt tcattctgtg
     4441 agacacaatt ggtcctgtgc ttacagatat tcccaattca tttaatgaaa cggcaaaaaa
     4501 cgtattattt tcttcttgag gttgctcgtt agtcgcaatg cggataaata gttctggatc
     4561 attaggttta atgacttcaa caaaagggac tgttttgctt tggtagtcac ggaaggtctg
     4621 gtcattagat tgagaaatct gaacgtctga ctgctgttta cccttagtca gtttaatgat
     4681 gatattttct tgtaagacat catcatcctt aaaagccatg tctctacttt caaagatatg
     4741 aatgtgctct atagcacact ccttcaaaat aaattctctg aatggcttgt aataaggacc
     4801 attacaaaat gatcttggaa taatcgctac aatttgccca tcttgttcca tgagttgaat
     4861 tgttagtgca acaaatgctg aataaagatt aactgtttca attccaacct ttctcaactc
     4921 tcttcgatgc tcagaattgc tattaatttt tttatatgga ggattaataa tggcatgagt
     4981 gtatcgctct cccttatccg aagagatatt ctcaacagct cctaaaatga agtcttgagc
     5041 atagatagag aaattaagct tcatagcatg catgttaatt tctagttgct cttgcatgat
     5101 gggatcaatt tcccatagat ctactagatt aatattctta agtttttttg cagcggaaat
     5161 agttaatgaa cctatacctg ctccacagtc tataagtttt atttcttttt tatgatcgtc
     5221 gaaaaggctt gccatatatt cagcaataac actaggcgtc atgaattgcc caagttcaga
     5281 ttttttcttg ggatttagct tagcattcgt agtctttctt atgtgctcga ccgtcataag
     5341 attcatggtt cgtattgctg acctaatgaa acaaaagtat cgcaagtata aacgtaaaag
     5401 gacgcttatt tttgatagca gttttattcg ggtttaaatg ctataaaatc catcattcag
     5461 cttcctgctg gaaacccatg aaacatccat tgtatgcccc ataaaatagc tagaattaaa
     5521 actacgagaa agatgatttc taatttcatc atttttaaat tcttctaaaa ttaacataat
     5581 acaccttata cgaattcaaa aatgggagcc taagctccca tttttattga tgatttttta
     5641 attcttttag catggcatcc cgcaaaattt cattcattcg tgtttgatag cctttgcctt
     5701 gagctttaaa ccatgcaagt acatcagcat ctaaacgaat ggaagtctgt tgcttcactg
     5761 ggcgatagaa ttgattatgg cgtacagcat tgctccagtc ggtaatttca ggcatatctg
     5821 agagatctag ctgatcgtca ggaaccgttc ccttagcaag caagcgttga atttctgcat
     5881 cttgcttgtc gctgaatttt tcattcagtt ctttgcgtga gtatctaacc atgctcatat
     5941 ctatctcgct ccgccttagt gacttgtctt gcactaatga ttcgtatgat ttcacagtca
     6001 tcttcatcaa agatggtgtg agctactaag agcattagta cgccttttac ttttccaatg
     6061 gtttgccaac gttcttcacc atcggtatgt ctgtcctgga ttgagatcct taatggatca
     6121 tcaaaaacaa ggcttgccgt ttcgaaagag atatcatgtt ttttctgatt ctttcgattc
     6181 tttgcctcat cccattcgaa atactgttcc ataaaaaaca ttcaaatttg tatatacaat
     6241 aatgtatcat aaagttgtat cactcaagtt gagaagcgta atttttagtc aaaattattg
     6301 gcaaaacatt ccaaaactgc tcataaaaaa gccgacttgt ttccaagtcg gcttttcggg
     6361 tttgcgtccc cgatttttta taacataact ttttgaaata taaaactatt tttatatgcg
     6421 tttcgtataa cgcccattat gttaaatgag taaaggacaa acatagatgc aacttgagct
     6481 ttattcgcac tgcattgctg ctttttgaag agcataaaaa aagcgttcat cttgtaatat
     6541 agcaggattt gttccccatt ccttaccata catttttgta gcatcatcat ttaatttatc
     6601 atatgcacag ctacaaatag acttatctgc caaaccttct gcacaccctc tgataaatgc
     6661 ttttttttct ttagaatcgc aagcagataa aactagtgtt aataatataa tagaaacggt
     6721 tgtaatagat tttttcattt ttactatctc tttgataatg ttaaataagc aacaaaagca
     6781 ataataaata acccccctaa aaatttacca aaagctggga accaaacagc aggccatatc
     6841 aagccacgac ctagattata agcaaaccct ttataatcat aatctcccca aaaattacca
     6901 taaatggctg tgaaaaatgc taaaaccaaa taccctacta ttacaatatg acttattttc
     6961 atgattttct ttacacttaa aaaacatgat tttacttgat ttttttttat ttttgaatgg
     7021 ctatcttttt ctaaagctta gtatatagag aatctataaa acaaattggc taaaaaatga
     7081 ttaaagctat taattcacaa tagttctcat cgagttaaaa aataatgatt taaataatat
     7141 taagctttcc taaaattaac ataatacacc ttatacgaag tcaaaaatgg gagccttaag
     7201 ctcccatttt ttaagattat tttaaaattt tgttgttgat atttggtcac tcaaattacc
     7261 aattcactat gactgcctaa tcgaactaat ttcaaaatta gcagttcacc ttcttctgtt
     7321 ttcttataga ttaaaacaag atctggtttg atgtggcaat cacgatgacc tacccaatta
     7381 ctgaccaaag catggtcacg atacttttca ggcaaaggtt catcagcttg taataactct
     7441 aaaacagttt gtagttcagt atctactgta tttcgatgct ggcctttttt ctcacgttta
     7501 tagtctttct tgaatgctga tgtgtactta atcgtccgca tttagatcag ccatcaagtc
     7561 ttttacagaa gtgaagctct ttgctttgct tagatcttct tccattgctg caatggtttc
     7621 agcattaggt tgccaaattt caaaaggcaa agccttttct ttagctactt tggtcaataa
     7681 caaacggact gcatctgaga cggttaagcc cattgttgct agaacttccg ttgcttcatg
     7741 cttaatgttt tcattgatgc gagtagttac tagtgcattt gaagccatgt taagctccta
     7801 tgattaacat tgttaatcac actatattcc tagatgacga aaagttcaat tcgtataaca
     7861 gccattatgt taaatttccg taagaatctt agtgcgatag agcagaatgt atatacaaaa
     7921 taagcttgat aacattattc aagaattaaa agaattatca gggaatagtc gtctaaacat
     7981 agagttacct gatgatattt ccatatctag ttatgaacaa aaaataggtt ttatttttcc
     8041 aaaagactat aagaaagttt tgaaagaggt tagcaatatc ttttatggaa caattgaatt
     8101 agcgtctctt acagatgaga aagaatacta tcgagggcta tctcagatat taaatgatgc
     8161 tagggagcaa ggtctgccag aagattggct acctatttgt gaagataatg gcagttatta
     8221 ttgtctatct ccaaatcata aaattagata ttggacagca gatggatata gcgatgagca
     8281 gtgggaagat ttagcagatt ggatcaagca agtttggata gatggaaatt aagtatttct
     8341 aaaattaaca taatacacct tatacgaaat gcttgaaatt tacttataag catcaatatc
     8401 ttagtcaaag ccgttctagg gttcagcgca ctggaacggc tttttcatga ttattcacgt
     8461 tccacgctta ttctttgatc aatgtgtcac aactttgatt agtacaccta aataagcctc
     8521 ttagccattc ggcaagaaaa accggcttaa ccatcctctt ttcggctgct ccggttcaac
     8581 ctgctccggc actggaatac gcttattttc cgtctgctcc ggagtagtca atccatcctg
     8641 tttagcttct gctgtgtccg gttctggagc tgttttggcc atgcttggtt gcagcggttc
     8701 ttgatcggta aacgtagtca tattggccgt gggaggcttc atttcaatga gcgttagaag
     8761 cgtatcaata cgagcattcg ccttggattc acgatcttcg gctttttcta actgtttttt
     8821 tagaaagtcg atttggtttt tcagctctac cactgtttta tcagtgtatt caggtgtatt
     8881 ttgattttgt attgaagacg tatcaatacg ttttgcctgt tttgctccga aaggctcacc
     8941 atagacgcga agtaattctg aaaaatctat aaaaccgtca tagtcacggc ttaatacacc
     9001 gttttttata tctttataga ttgtcgctcg ggatttttta tacagagttg cagcatcact
     9061 aacactgtat tttgtagctg tattcatagt gtattgctgt gtattttgaa aacgtattgg
     9121 agaatacaat atgtgtattc tctaatacac tcaagcttta aagcctaggt tttttaggtg
     9181 aggtaaaaac tgcttttgtt gtattggatc tcttagcatt tctttgattt ttgctgctag
     9241 gctctcatag ctttcattac catttgctaa gttcgaaaat tcaggcaatc gtgaaagctg
     9301 attgccaaac atattgattt gagcatctgt catttggtgg aatgctgttt gggatacctt
     9361 tgttaattct tgttgagatg gcttatcttt cgatttaatt ctaaattgga tatctgtgat
     9421 aactcgccct ttcttgaatt ggtcatagtc aacggtaata ttagtatgtt tattaatttc
     9481 acttattgct ctgtctaaga cattagattt aaagttcgac atagttgaat attcagtcgc
     9541 atccagccct aattttgatc taagatcgtt aatactatat ttctcagttt ttgctacatt
     9601 caaccattta attaatagct catatagtct gatgctatat ttactcttaa agtcagacac
     9661 ttgttctata tggtattttg tgaattgttc gcttaatcgg cttattaagc tgataacttc
     9721 gttactaagg tataaaacga taaggccact agaatctaca taggtcactt tgtttaccca
     9781 atttgctgta gtaagagctt cttggccctt atatctatct aaataactaa actcccttct
     9841 ttttagagtt ttcgctgctt ctctaagcaa gccataggca ttattactat ctactttaaa
     9901 ttgatgaacg tagtcactta cgaaaacttc tattggtgta tcagatgtga gattagaaga
     9961 ctctctagct ccaactatgg ccaagagcat aatacgttgc tctatgagtg ataagttgaa
    10021 agaggcatcg ataagagcat tatccttaac aactaattta ttgttatttg tcatgatatg
    10081 aagcttatta attcatttaa ctaggttata tgttatatat tttaaatagt caataatcta
    10141 gctatttaaa taccccttaa acctatcttt atcacccctt aaacctatct ttatcacccc
    10201 ttaaacctat ctttatcacc ccttaaacct atctttatca ccccttaaac tattgcaaat
    10261 aaaggcttac agcatctcta aaagcattta aaaactttaa aattattaaa agccttagga
    10321 gggaaaaatt gcccttggtt ttcgctacgc tcaaactcta ttgaatctcg ctttcgctcg
    10381 attcgtgggg caattttttg gatcattctg ttgtgacttt gagaaaaagc aaaggcaaga
    10441 gcaaaagtaa ctcggaattc gcttcgctca taaagctttt ttactcgcta cgctcgatct
    10501 agactcaatt ttctgcatgc gatttacgct tatttaggct tcacagccta aaaagctgtt
    10561 tttcagagag tctaggtgcg aattttggtg cttcgctcgt agacactcgc tctattcatc
    10621 ccgattagaa taaaaatagc ttctatttgg cgctcatttg cgttttttga cctgattact
    10681 catttcaata gtgagattat gtcttaagta aaaatgtggc tgaaatcgca tttgagagcg
    10741 tttcaacttg ggttttattt ttgatctgta ggctagagtt tccgcttata gattttttcc
    10801 cttagacaca caatagtcag gatctctttt ggccaagcac tatctttact gtcagttaag
    10861 tcaattaaat ggattgggag ccggtacata ccggcaattt ttggttcaca caccacgcat
    10921 ctaaagataa aggctccaaa gccttatcct ataagggata cagcgatttt tcatctatct
    10981 ggaaagcgtt tacgctttta gcatgatttt tattattttt cataggttta gctgtaaaca
    11041 ctttttattg cgaataaagt gcaatattta cttacaaaac aataggatag cttgtaatca
    11101 cctttgttta cgcttttata tgaatttctc tttttatatt agatatttaa cctgtaatca
    11161 ttaaaactta acacagtgtt tacttatatt tgatagttac tttttaaaaa ttaggtgctt
    11221 agattgaaaa caaattgtag gagtttttca tgaacagtac tcgaaccgtt gccgtcagat
    11281 tagacaatca ggtcgcacaa gaatatgagc gtttggctca gttgaagggt gataaattag
    11341 ggacttatct gcgggatctt ctgaccagta atttacagat ggccagtatt gaacggcagg
    11401 ccaatcggat tgaaacattg gtgaatgatt ttgagaagca aattaattac tccctcgata
    11461 agtttactga ggccaattat ttaaatgaaa aattctttga agactttggc catatatata
    11521 tgtttcaaat tgggatactg atgcatctaa aagtcgatcg aggagacatc caaggaatgc
    11581 aaacaaaagg aaatgcatat gcccaaaaga actattttgc ggggaaaaag ccatgagcaa
    11641 tattattcaa ctctctaaac catgtgcttt ctgtgattca agggaaaacg tacagatttt
    11701 ttcagggctg atgctttgtg ggaattgtca ggacaatatc cggattacca atcctgaacc
    11761 gtaccgggtt tgtcggagac cacttttctt gagagaatgt cccgatgaaa aaagtaaaat
    11821 acacccctga aatcagagat agagcggttc aattattgat tgaatctgaa aaagattatc
    11881 cttctacttg ggctgcaatc accgctattg ctcccaagat aggttgtact cctgaaactc
    11941 tacgtgtttg gtatcaaaaa tatttagata aacaaaaccc aattaaagta cagcaacttt
    12001 cagatcaaga acgtattaaa caactcgaac gtgaaaataa agaactgcaa cgtgctaacg
    12061 aaattctacg taaagcagcc gcttttttcg cccaggcgga gctcgaccgc ccacacaaat
    12121 aatggtggat tttatccaga acaataaaga tcgatatggt gttgaagcga tttgtagaat
    12181 tttaccgatt gcagcttcga cctattatcg ggctttagat ctcgttgata acccagaaca
    12241 tcgagcgaaa cgtgctctgc atgatttaca tcatgcagag caaatcaaac gtatttggaa
    12301 agaaagttta ggtcgatatg gtgtgcgtaa ggtctggcaa caattgaaac gtgaaggtta
    12361 tgttatcgca cgttgtacag ttgctcgatt gatgcagaag ctaggtatac aaggtgtttg
    12421 gcgtggtaag aataaacaaa ccacccgtag ccgagatgat caaaaacgag cagatgactt
    12481 agtaaaacgc aattttaatg cagatcatcc tgaccagctg tgggtcgctg acttcacgta
    12541 tattcaaact cattcaggct gggtctatac cgcctttatt attgatgtgt tctcgcgagc
    12601 aattgttgga tggaaagtat caacacggat gaatacagat atggtgctcg atgcactgga
    12661 gcaagcattg catgatcgag gcatgccaaa gaacgtgatt catcatagtg acagaggcgt
    12721 gcaatatctt tccattcgtt ataccaatcg tttagaagca gcaaatttac gagcatcagt
    12781 cggtacgacc ggtgattcat acgataatgc tttggctgaa acggtgaatg gcttatacaa
    12841 aacagaggtg attgaatatt taaaagcgga ttggcaaggt ttagcggatg tacaacttgc
    12901 gacactaaac tgggtagatt ggttcaataa agagcgtgta cacagtgcac tgggttatgt
    12961 atcacctttt gagtttgaag caatgtacta tgataagatt aacccgttag gtcaggtggc
    13021 ctaacttaaa taaaaaagtc tccgacaaac ccggtacggt tcaatcctgg tatgtttgag
    13081 gccaatgatc agattgaaca aaaaaaccag agttaatctg gttttttatg cagtacttta
    13141 tttttatgta cgaaaaactg tattgctatt gaagttgtag aaccgactaa gcctatagta
    13201 atcatgccac ttatagcaag ttgatgaagt tgttcatatt ctttgatcat ggggaattta
    13261 tgtaaaaagt ggaatagccc taacatacaa cctaaaatag tcgaaaacat tagatagtaa
    13321 aaatctgtga ctgcactgat cagcctgttc aaaataggct gtatgttaag tttcgagtgt
    13381 gtagtcaagt gatttacaaa gcataaaatt cccataacga taaataatcc aatactagta
    13441 tctataagat tttcgatcgt aaaaaatgca aaaactaagt taaatgactt actgaaagat
    13501 tcttcagttg caaacttttg actcgaaata aatatggaaa aataagaacc aaaggttatg
    13561 cataggaaaa cgaaaaactc ttggaatatg aatgtcggga ggtaaatttt tttctcaaca
    13621 tggaataggt cttcatcatt tgataaatat ttaatttcag ggtctttttc ttcattcata
    13681 agctgataat agaaatgcga atatatgtta ataggggcgt gttaaacgaa aatttgaaat
    13741 tcagaaagta aaaaagctag cccattagac tagcttctta accttgaaat tatctttcc
//
