LOCUS       NZ_CP055286            14653 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH4, complete sequence.
ACCESSION   NZ_CP055286
VERSION     NZ_CP055286.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 14653)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14653)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055286.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14653
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH4"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            complement(join(14645..14653,1..351))
                     /locus_tag="HUK62_RS18455"
                     /old_locus_tag="HUK62_18455"
     CDS             complement(join(14645..14653,1..351))
                     /locus_tag="HUK62_RS18455"
                     /old_locus_tag="HUK62_18455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4829 domain-containing protein"
                     /protein_id="WP_224223039.1"
                     /translation="MATQTTILFFNSLAKHDLESAIKYVWPDARLHEDLKSSERFLSFK
                     DSKILEVVRINYDSAESRPEYYQEFYKIISVMVKVKVVHIDDAGSPVGDYILFITLVKQ
                     SPQSDWLITEFGSGP"
                     /name="DUF4829 domain-containing protein"
     gene            complement(450..743)
                     /locus_tag="HUK62_RS19195"
     CDS             complement(450..743)
                     /locus_tag="HUK62_RS19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005406274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228723781.1"
                     /translation="MLSFVLTYLSLFHQKYTLIDRIVVDKLGKVEVIGGGFPLQFLVDG
                     EISPGGSIALDPLNIIIGIDQFIFLYFILDYLFWLMCLLVGYAFFRKIKVKL"
                     /name="hypothetical protein"
     gene            complement(<795..1184)
                     /locus_tag="HUK62_RS19200"
                     /pseudo=""
     CDS             complement(<795..1184)
                     /locus_tag="HUK62_RS19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1480..1791
                     /locus_tag="HUK62_RS18465"
                     /old_locus_tag="HUK62_18465"
     CDS             1480..1791
                     /locus_tag="HUK62_RS18465"
                     /old_locus_tag="HUK62_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_057069502.1"
                     /translation="MKIEFSWDEHKAEINLKKHGVAFEDATLVFYDAHALYKQDRFENG
                     EYRWQAIGMVHGQAVLLVAHTVQDHNGMDHIRIISARQAEKKERKQYEQNRYLQNGFE"
                     /name="BrnT family toxin"
     gene            1754..2068
                     /locus_tag="HUK62_RS18470"
                     /old_locus_tag="HUK62_18470"
     CDS             1754..2068
                     /locus_tag="HUK62_RS18470"
                     /old_locus_tag="HUK62_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_057069499.1"
                     /translation="MSKTVTYKMDLNNPPVLSEEQKARLEALAKRPDSEIDFSDIPELD
                     ESFWKNAVQNPFYKPTKQVTTVRIDADVMQWLKAQGKGYQTRMNKILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            2271..3542
                     /locus_tag="HUK62_RS18475"
                     /old_locus_tag="HUK62_18475"
     CDS             2271..3542
                     /locus_tag="HUK62_RS18475"
                     /old_locus_tag="HUK62_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="WP_004763336.1"
                     /translation="MDHNDFKEELSETGNPVGIEFEDEDESTLMEPFDPEKISIEQRVV
                     AMETVIRRLKQGLINLSPAYQRNEVWDDTRRSRLIESLMLKIPLPMFYVAASDNGSWEV
                     VDGLQRLSTIKKFIIEKSQNRLVLKNLEFLGDKFDKHDYTALEGQADYPKYLNNLLETE
                     LRFTVINPSTPEAVKRNIFKRINTGGMPLTLQEIRNALYQGKSTELLFELANSPEFLNT
                     IKTKVNDTRMAARELTLRFIAFNLFSYNVYPSFTLDTWLTSSMQAINLMPLSNDSDINL
                     KKIEEKIGSTLTQSIKVYNVEQLRQNFTLAMQRAEIIFGKYAFRKIGTNSSKIAPINRY
                     LFESWSNILVDLSEDDFNKLLDEKELFEENLNKLNTNFSYVNSIGKYASQGKYVLYRYK
                     ILSYLIRETIDSNIISLDSNEWTV"
                     /name="DUF262 domain-containing protein"
     gene            3539..4597
                     /locus_tag="HUK62_RS18480"
                     /old_locus_tag="HUK62_18480"
     CDS             3539..4597
                     /locus_tag="HUK62_RS18480"
                     /old_locus_tag="HUK62_18480"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="WP_004763337.1"
                     /translation="MISKLKIQNFKSYVNSEIELNRLTILSGLNNSGKSSIIQAIRMVY
                     RSSLGKSPYLNDYGAYEELWCKYSPKGNSIVLEFEGIEDAKLEISPSKHISAKNAPLIS
                     YIGANRLGPQKTLPIQYQPIMYNYLGENSEYVLDIIKALEFSKIPENLHHSNSAATSLI
                     YEIQGWLSEISPGVNFTFEIDNKSDLGKSEFDSYRPNNVGFGLSYALPIIVAGLALSSE
                     PAQGNENEEEKIANWYQQKAQRGALLIVENPEAHLHPRGQTKLGIFLSKVASCGVQVII
                     ETHSDHLVDGVRIAVKDQLIDHENTSFYYLDKKDNLSHLTKINIDRNGRMSEWPNGFFD
                     EALKNRSYLIRN"
                     /name="DUF3696 domain-containing protein"
     gene            4599..5498
                     /locus_tag="HUK62_RS18485"
                     /old_locus_tag="HUK62_18485"
     CDS             4599..5498
                     /locus_tag="HUK62_RS18485"
                     /old_locus_tag="HUK62_18485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004763338.1"
                     /translation="MNNEFIFNENSSVSSYSDNLISIIENMFEGLVSIGSFGDTYTLYA
                     DNVDRLFDICFAEDYKLKDYYDLCFTHNREMAFFLLELIDKSPLINHIDNRKLDELTSK
                     TFYVDGLTLKNEIPIVFSALYNTYLLSLGTDPIWTKNEIIVSQLDEDFIYNGALINALN
                     ISSKTNSIFFNSLNNDDQTLEKLDPNAIFHEKMTEWYLALIPENKTRLYEKLKFIFSNN
                     FNVSSALIKPITRNFKNKPVTFSEIRLDTISGGTMRIFFKKINSNPYFFIGWTKKDDNE
                     GYTENHKSACEILEKIQI"
                     /name="hypothetical protein"
     gene            complement(5568..5831)
                     /locus_tag="HUK62_RS18490"
                     /old_locus_tag="HUK62_18490"
     CDS             complement(5568..5831)
                     /locus_tag="HUK62_RS18490"
                     /old_locus_tag="HUK62_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004763339.1"
                     /translation="MSTVDLIDEDDNESGIAAKAANVLRDRFIAAAQSGPVMYVENDTV
                     LLKELNGHPIVIKHLSGRNPELAQRVASRGTFKIKKRKVSQD"
                     /name="hypothetical protein"
     gene            complement(6653..6946)
                     /locus_tag="HUK62_RS18495"
                     /old_locus_tag="HUK62_18495"
     CDS             complement(6653..6946)
                     /locus_tag="HUK62_RS18495"
                     /old_locus_tag="HUK62_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004763342.1"
                     /translation="MPKYPDDDYLSRQEIDDLRSEAKHAAKYMKAFFDFKIEARYSFSV
                     NWSAEDREFVGLCAEFPSLSWLDPDSDKARSGIERLVFDVLQDMSSTGEAIP"
                     /name="hypothetical protein"
     gene            complement(6980..8122)
                     /locus_tag="HUK62_RS18500"
                     /old_locus_tag="HUK62_18500"
     CDS             complement(6980..8122)
                     /locus_tag="HUK62_RS18500"
                     /old_locus_tag="HUK62_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228127544.1"
                     /translation="MIYYTTTKNDCLLSLMQCVSNGKAKFWFSDVVPFSKFQNLIPKLI
                     LEYGLDLGEQARKKKSDYGEPVWSLVINYDPAKSEVFQFWLFSTGYREARRSKLTLQEI
                     LAQNSSMLRKQKLNSILTTKKEKLLRFGAYVLGQYIDFMDLKPQFSRVYYHPERFGVIF
                     NVHAKQMKTIDSNRKHVYRLFQPFNAFEEKRFESIQRIFGFAFLENEHTHWNQASVSEF
                     LLRRFGIQFDPEVSYNERLKALTGALRGVRKKHLDFFQRHSQKKIRFTWYLSEEFMQSA
                     TWELNKKLDLISTEKADRLKEATFRLSSKGNFHGTRHQIGTLQARMRSILNARDPAQKK
                     LNQMYFPKNLHYVRFTAKKNCNMKEFELACRNADTMYLNK"
                     /name="hypothetical protein"
     gene            10945..11853
                     /gene="repM"
                     /locus_tag="HUK62_RS18505"
                     /old_locus_tag="HUK62_18505"
     CDS             10945..11853
                     /gene="repM"
                     /locus_tag="HUK62_RS18505"
                     /old_locus_tag="HUK62_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131200.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004763412.1"
                     /translation="MTDLVVKSNKLILALQSLTLSEARLIQLAIIDARETGKGLTTEHP
                     LELNAARYAKAFNTSLDAAYLSLSEAEETLFKRQFTIENDDGSLTKSRWIQDANYKKGE
                     GRILLTLTRVLIEQVTNIDGFETYFTSYYLKQTAKFKSIYAVRLYELLKQWKSVGYTPT
                     FNIDKFREQLGIGINEYSRVEAFKRRILDASIQQINKFSDIEVEYQQHKKGRSIVGFSF
                     TFKHKETQKNSKINNVINELTDKQILLFSNKLAHNIPFSSKYSKPGESYEEFAERLANE
                     LIDPQKVKLYKPYLEEVGFKS"
                     /name="repM"
     gene            11938..12579
                     /locus_tag="HUK62_RS18510"
                     /old_locus_tag="HUK62_18510"
     CDS             11938..12579
                     /locus_tag="HUK62_RS18510"
                     /old_locus_tag="HUK62_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_004763413.1"
                     /translation="MIILVGGEKGGSGKSCLAQNLAVYLQQKKRDILLLDADPQGTTTD
                     WIKERDENPDLINIPSVQASGNIRQVLDDLSRRYQDIVIDAGGQDSEALRSAMTIATHM
                     LLPFRPKRRDLKTLVHVEQLIKLAKAVNPELVTKAVITQCPTLPSQAQRILDAKDACES
                     FGLVALNQFTTNRNVYDDADENGLSVFEVDSDPKAKAEIERIVIEFLGEL"
                     /name="AAA family ATPase"
     gene            12576..12869
                     /locus_tag="HUK62_RS18515"
                     /old_locus_tag="HUK62_18515"
     CDS             12576..12869
                     /locus_tag="HUK62_RS18515"
                     /old_locus_tag="HUK62_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034428027.1"
                     /translation="MMALKGLKKADQDTKQQDIDNFISGASKRVKTLEKSQQKYLRLTF
                     SLTKDVDKEIDDLLVDSKVARANRSIIVKAAIHHLQQLPKDELKNLILKELN"
                     /name="hypothetical protein"
     gene            13711..14268
                     /locus_tag="HUK62_RS18520"
                     /old_locus_tag="HUK62_18520"
     CDS             13711..14268
                     /locus_tag="HUK62_RS18520"
                     /old_locus_tag="HUK62_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404813.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_004763415.1"
                     /translation="MPNQVGYIRVSSSEQNTERQLDGIHLDKTFIEKASGGSRERPQLL
                     AMLEYLRDGDTVHVHSIDRLARNTNDLNDIVNSLNDRGITIIFHKENLTFSHDVAQSAI
                     NKLMFQMLAAFAEFERSMIRERQKEGIAKAKAKGLYKGRKRKVDYAEIQKAMQEDGSTF
                     RSVAKQFGVGIATVQRAVGQKP"
                     /name="recombinase family protein"
ORIGIN
        1 actaccaaat tctgtaatta gccaatcaga ttgaggagat tgtttcacaa gagtaataaa
       61 taaaatatag tcacctactg gactaccagc atcatctata tggacgactt taacttttac
      121 catcactgaa ataattttat aaaattcttg ataatactca ggtctacttt ccgctgaatc
      181 ataattaatc cttactactt ctaaaatttt tgagtctttg aaagagagaa acctttcaga
      241 actttttaaa tcttcatgta atcgcgcatc tggccaaaca tatttgattg ctgattctag
      301 atcatgttta gcaagtgaat taaagaaaag tattgttgtc tgtgttgcca tctctttatc
      361 atgatttaat ttctgtttgt atgaattaat gattagaagt agacatagac ctacaagtaa
      421 aattcctaat tttataaata ttttagacat tataatttca cttttatctt tcgaaaaaac
      481 gcataaccga ctaaaagaca cataagccaa aataaataat caagaataaa atataaaaaa
      541 atgaattgat ctattcctat aataatattt aaaggatcta gcgctatact accgcctgga
      601 gatatttcac catctactaa aaactgtaat ggaaatcctc ctcctataac ttcaactttt
      661 ccaagcttgt ctacaactat ccggtctatc aatgtatatt tctgatgaaa caaagacaaa
      721 taagtcaaaa caaaagataa tatattgaaa tatattattt ttttctttaa taattcttta
      781 actttaaaat cagttaaaaa taaccaataa gacaagcctg gaattagagc cataaagata
      841 gtccaagtgt cggtatacat cttccaccat tctttcgaaa cttctcctgt atggctccat
      901 ccaaaaagaa caaaaaatgg catagcaata attaaagagc taatacagat agttagcaaa
      961 ttaagaatac tataacgatt taaaagaatt gagaccataa agctaaaagg tactgtcaaa
     1021 atactataag ccaagtaaac aagaccatgc cctgcaaaaa ccacaaaaat tgaatataga
     1081 ctatattcac gattcattat gataaatagc aatgctgtga aaaaaagtag tggtaaagga
     1141 gcaagtaata gggcttttac tattttttct tttgagtatt tcattaactt aattctctta
     1201 tctactcaat aactattcat actataccga ttttcttgga ggtcgtttgc ttccatttaa
     1261 cataatgggc attatacgaa acgtgttttt aatattactt taagatcagt atcttatcgt
     1321 tattcatacc ctctggaacg ttctcgttca accgacttgg aaacaagtcg gtttttttat
     1381 gagcggtttt gatatattcc gccaataaaa attggctaaa ttttgcaaaa tttacaattt
     1441 tgtattaaca tttttatatt aacaaaaagc gatgcaacta tgaaaattga gttttcatgg
     1501 gatgagcata aagcagaaat aaaccttaaa aagcatggtg tagcttttga agatgcgaca
     1561 ttggtttttt acgatgctca tgccttatat aaacaggatc gctttgaaaa tggtgaatat
     1621 cgttggcaag cgataggtat ggtacatggg caagctgttt tattggtggc ccacacagta
     1681 caagaccata atgggatgga tcatatccgc attatttcgg cacgacaggc agagaaaaaa
     1741 gagaggaaac agtatgagca aaaccgttac ttacaaaatg gatttgaata atcctcctgt
     1801 tttgtctgaa gaacaaaagg caagacttga agccctggct aaacgcccag acagtgaaat
     1861 agacttttcg gacattcctg aacttgatga aagtttctgg aaaaatgcag tacagaaccc
     1921 tttttacaag ccgactaaac aagtgactac ggttcgtatt gatgcagatg taatgcaatg
     1981 gctaaaggcc caaggtaaag gctatcaaac acgaatgaat aaaattttac gggatgccat
     2041 gctaaaagaa ttaaaaaatc atcaataaaa atgggagctt aggctcccat ttttgactgc
     2101 gtataacgtg tattatgtta attttaggaa attttaacta taagataaat aattcactat
     2161 gagtaatttc aaatagtaga attacccttt tcttaaaaac ttttatatct cttaaagcta
     2221 taaatataat gatatactta tatcatttta aaatttatag agtttttaac gtggatcata
     2281 atgactttaa agaagaattg tcagaaacag gtaatcctgt aggcattgaa tttgaagatg
     2341 aagatgaatc caccttaatg gaaccatttg atccagagaa gatttctatt gagcaacgtg
     2401 tagttgccat ggaaacagta attagaagat taaaacaagg cctaataaat ttaagcccag
     2461 catatcaacg taatgaagta tgggatgata ctcgtcgaag tcgattaatt gaatctttaa
     2521 tgctaaaaat ccctttacca atgttctatg ttgcagctag tgataatgga tcatgggaag
     2581 ttgtcgatgg attacagcgt ttatccacta taaaaaaatt tattattgaa aaatcacaaa
     2641 atagacttgt attaaaaaac ttagagtttt taggtgataa atttgataaa catgattata
     2701 cagccctcga aggacaagca gattatccaa aatatttaaa caatttatta gaaacagaac
     2761 ttagatttac ggtcattaat cctagtactc ctgaggcggt taaaagaaat attttcaaaa
     2821 gaattaatac aggtggtatg ccattaactc tacaagaaat aagaaatgct ctttatcagg
     2881 gaaaatccac agaattactg tttgaattag caaactcgcc agagttttta aatactataa
     2941 aaacaaaagt taatgatact cgtatggctg cgcgtgaact aactctaagg tttatagctt
     3001 ttaatctatt ttcgtataat gtgtatccta gttttacttt agatacatgg ttaacatcaa
     3061 gtatgcaagc tattaaccta atgcctctat ctaatgattc agatattaac ttgaaaaaga
     3121 ttgaagaaaa aattggttca actcttactc aatcaatcaa ggtctacaat gtagagcagt
     3181 tgaggcaaaa tttcacttta gctatgcagc gagctgaaat aatatttggt aagtatgcat
     3241 tcagaaagat tggaactaac tcttctaaaa tagctcctat taatcggtat ctttttgaaa
     3301 gctggagtaa tattctagta gatttaagtg aagatgactt caataaacta ttagatgaaa
     3361 aagaactatt tgaagaaaat ttgaataaat taaatacaaa tttttcctat gttaattcaa
     3421 ttggtaaata cgcatcccaa ggtaaatatg ttctttatcg gtataaaata ctttcttatt
     3481 taattagaga aacaatagac tctaatatta tctctcttga ttcaaatgag tggactgtat
     3541 gattagtaaa ctaaaaattc aaaattttaa gtcttacgtt aactctgaga ttgaattaaa
     3601 tagactaaca atattgagtg gattaaacaa ttcaggtaaa agctcaatca ttcaagcaat
     3661 tagaatggtt tatcgtagtt ctttaggtaa atctccttat ctaaatgatt atggtgcata
     3721 tgaagaatta tggtgtaaat atagccctaa aggtaattca atagttctgg aatttgaggg
     3781 tatagaagat gctaagcttg aaatttcccc ctctaaacat atttcagcta aaaatgctcc
     3841 tcttatttct tatattggtg caaatagatt aggtccacaa aagactttac ccattcaata
     3901 tcaaccaatt atgtacaatt atttgggtga aaactctgaa tatgtgttag atattattaa
     3961 ggcattagaa ttctctaaaa ttccagaaaa tctacatcat tcaaatagtg ctgcaacctc
     4021 tttaatatat gaaattcagg gatggttatc tgaaatttca ccaggtgtta acttcacatt
     4081 tgagattgat aataaatctg atttagggaa atctgaattt gattcatata gaccaaacaa
     4141 tgttggattc ggtcttagtt atgcactacc gataattgtt gctgggttag ctttatcttc
     4201 agaaccagct cagggtaatg aaaatgaaga agaaaaaata gctaattggt atcaacaaaa
     4261 agctcaacga ggagctttat taatagtaga aaatccagaa gctcatctgc atccacgagg
     4321 tcagacaaaa ttaggaattt ttctttcgaa agttgccagt tgtggtgtac aggttattat
     4381 tgaaactcat agtgatcatt tagtcgatgg tgtacgtata gctgtgaaag atcaactcat
     4441 tgatcatgaa aatactagtt tttattactt agataaaaaa gacaacttaa gccatcttac
     4501 taaaattaac atcgatagaa atggacgtat gagtgaatgg ccaaatggtt tctttgatga
     4561 agctttaaaa aatagatctt acttaataag aaattaacat gaataatgag tttatattta
     4621 atgaaaatag tagtgtttca agttattcag ataacttaat ttctattata gaaaatatgt
     4681 ttgaaggact agtatctatt ggttcatttg gagatacata tactctatat gctgataatg
     4741 tagataggtt atttgatatt tgttttgcag aagattataa attaaaagat tactatgatc
     4801 tatgctttac tcataataga gaaatggctt tttttttatt agagctaatt gataaatctc
     4861 ctcttataaa tcatatagat aatagaaaat tagatgaatt gacaagtaaa acattttatg
     4921 tagatggctt aactttaaag aatgaaattc ctatagtttt ctctgctcta tataatacat
     4981 atttattaag tcttggaaca gatcctattt ggacaaaaaa tgaaattata gtctctcaat
     5041 tagatgaaga tttcatatat aatggtgctt taataaatgc tttaaatatt agctctaaaa
     5101 caaattctat tttttttaac tctttaaata acgatgatca aactctagaa aaattagatc
     5161 ctaacgcaat ttttcatgaa aaaatgacgg aatggtatct agcattaata cctgaaaata
     5221 aaactagact atatgaaaaa ctaaagttca tattttctaa taactttaat gtatctagtg
     5281 cattaatcaa gcctattact aggaatttta aaaataagcc tgtgactttt tctgaaatta
     5341 gacttgatac tatttcaggt ggaactatga gaattttttt caaaaaaata aatagtaatc
     5401 cttatttttt tataggttgg actaaaaaag atgataatga aggttataca gaaaaccata
     5461 aatcagcatg tgaaatttta gaaaaaattc aaatttagtc gatagcataa tcgctattta
     5521 acataaaatc tcttatacga aatttgaatt gtaagtccca gttagcctca atcctggctt
     5581 actttacgtt ttttaatttt aaaagttcca cgactcgcta ctcgttgagc aagttcagga
     5641 ttacgtccag ataggtgttt aatcacaatt gggtgaccat tgagttcctt aagcagtaca
     5701 gtatcgtttt caacatacat aactggtcca ctttgagctg cggcaataaa gcggtctcga
     5761 agtacatttg cggctttggc agcaatacct gattcattgt catcttcatc aattagatct
     5821 actgttgaca tagtgccgca ctcccagtta gctgatatat gaatcatatt acaagtcggg
     5881 tagacaggat aaagaactag agacctttct acatataatg ctctgatcga cacacacaat
     5941 agcttctttc cacattaagg taggtattat gtgtgatctt aattttatgt ttaatttcaa
     6001 taacttaaaa aagttaatgt ttgagtttta tttttaccat ctaaatacaa taatatttaa
     6061 aatcaaatac ttatatttta taaaaaattt aaagcttagc aacacacatc atagctacct
     6121 aatttgactt aatcaggtag ctatgatgtg tgttggtatt aaccatctta ttgaatttca
     6181 gtactttact aacaatttaa ctctctataa ttttaataat tttttatgaa gccagctatc
     6241 atccgacaca aaatttctac ccatttttaa tcctggatat ttaaaatttg tagggagttc
     6301 gtcattcaaa aatttagctg ccagagaacc aaatgaatca tataattttt cagaaattag
     6361 aaaaaatgaa taggcactgc aatacttgtg ctaaatctta tgcacttttg actttgttga
     6421 ctctaatcac tttttttatc tattttttta atcatttcac attagaaatt ctctttacag
     6481 accccaatgc cctattaccg gtgatcaaaa tggaagcgtt ggcacttggg ataatgcaca
     6541 ttgtttactg cttctttttc aaatatgtcg ataaaaaact tcaaatattc ggcttagatt
     6601 caaataatct gaatgaatac atccagcgga atcaggcatt tttccaaaaa tattaaggta
     6661 tggcttctcc agtggaactc atgtcttgta atacatcaaa gacaagtctt tcaatgcctg
     6721 agcgtgcttt atcgctgtcc ggatctagcc aggacagcga aggaaattcg gcacaaaggc
     6781 caacaaattc tcgatcttcg gcggaccagt tcacgctaaa tgagtaacgg gcttcaatct
     6841 taaaatcgaa aaaagctttc atatattttg ctgcgtgttt ggcctcactc ctcaaatcgt
     6901 caatttcttg gcggctgaga taatcatcat caggatattt tggcatagtt cacctttaat
     6961 ctttaagttt ttgcttattc tatttgttca aatacatcgt atctgcatta cgacaagcca
     7021 gttcaaactc tttcatattg caattttttt tggctgtgaa tcttacatag tgaagattct
     7081 tgggaaaata catctggttc agtttcttct gtgctggatc tcgagcattg aggattgatc
     7141 tcatcctggc ctgcaaagtg ccgatttgat gacgtgtccc atgaaagttt cccttagaac
     7201 ttaacctgaa ggtggcttct ttcagacggt cagccttttc agtagaaatt aaatcaagtt
     7261 ttttattcaa ctcccaggta gctgattgca taaactcctc tgacaaatac caggtgaatc
     7321 tgatcttttt ttgagaatgc cgctgaaaaa aatccagatg ctttttacga acgccacgta
     7381 atgcaccggt cagtgctttc aaccgctcgt tataactgac ttcaggatca aactgaatcc
     7441 caaatcttcg caacaggaat tcactcactg atgcctgatt ccaatgtgta tgttcattct
     7501 ctaaaaaagc aaatccgaaa atccgctgaa tactttcaaa acgtttttct tcaaaggcat
     7561 taaaaggctg aaacaggcgg taaacgtgct tccggttgct atcaatggtt ttcatctgct
     7621 tcgcgtgcac attaaaaatc acgccaaagc gttccggatg ataatagacc cgagaaaact
     7681 gcggtttaag atccataaaa tcgatgtact gtccaagcac ataagcaccg aagcgtaata
     7741 gtttttcttt tttagtcgtc aaaatactat tcagtttttg cttgcgtagc atactcgaat
     7801 tctgagcgag tatttcttgt agcgtcagct tgcttcttcg tgcttcacga taaccggtgc
     7861 taaataacca gaactggaac acctcacttt ttgccggatc ataattgatc acaagactcc
     7921 acactggctc cccgtagtct gattttttct tacgcgcttg ctcaccgaga tccagaccat
     7981 actctaaaat tagtttcgga atcaggtttt gaaacttcga aaaaggcaca acatcagaaa
     8041 accagaactt tgcttttcca ttagaaacgc actgcatgag actaagcagg cagtcatttt
     8101 ttgttgttgt gtagtagatc ataaatcaac caaaacctgc gttttttgcg gaaagcataa
     8161 aagtctatct tttattttaa agaatcacaa aaccattttt cactaaaaat ggtttttttt
     8221 tgagcgaaga tggtttttct gtaaataaga gttcctgtgg attcgtgctg atagtggttt
     8281 tcggggttgt ttttacggta tcagcggctt attctgtgtc tgccaaatat gatttttttg
     8341 ggatcgatgt gattcgtatc tgtcccgaat ttttctaaac ccttgtcatt gcggttttat
     8401 gtgtatacgt cagccaacgt atactaaaac tcgcaaagct gcgtgcgtga gaaatctagc
     8461 tggcgcggct atgacgggca ctaggtggtg aaattccgct tattgaccca atcctgattg
     8521 catatcatat aaacgtaaat ctgcttttaa gctgaccagt cgttgatatg taatctttgt
     8581 aatagttggg cgacatagaa acgggaatct aggcgactag atcaaaccct gtctaatgga
     8641 gagtgtgagt aacttcaagg attgagagtt gcgttaacaa cacctatttc atgcttcaat
     8701 ttttacgaag ggcatgatgt atggcgcaca ggtgggctgc tgagaatatc gcgaggtatt
     8761 agggagttga ggagaaatct ggaaacttcg gtactcaagg attaacttga atgacttgtc
     8821 gagatgacat ttcaggttag ttggtgagat cgctggatag ctcaagcctg taacggtgac
     8881 acgtagaacg ttatgtcact cagaaattaa gactgcattg attaagttca ttgcggtctt
     8941 ttttattttt tagtgattta aacatagcga agatgttgct ttgcgtaggt caagtgcttt
     9001 acccacaatg aagtcgaata acggtgaata atacgggttt ttggaactct taccgtagaa
     9061 caacggaatc gctgttattt tgtggagtgg ataagcgtac tgatcgttta ttaatactgg
     9121 tttactttta ttaatgaaca aagaccatta tgactaatat gggaatctct caagtcgatc
     9181 attatacaag gtggctcctt gatatagact tttgaagcga aaacctgcct tgagtgggga
     9241 ataagttttg attcatgaga gagggagagt tgttgagtag attctgtagc agacaatgat
     9301 acagatccag tcacttcgag cgaataaaaa cgtaagcggt gatgaagcga aagacaatgc
     9361 gggaaatatt taaaaccact gacaagggtt tttaaagtga ggtgtggcag cctcggtatc
     9421 tatgaaatgg tacgaacttt tttggcgtgg aagaaaatca aaatgattat ttcaattatt
     9481 aacgtggctg ttagtagttg tgatatttga tgattattca gtaagaagca tgaacggtgg
     9541 ctccgttgtt ggttcacgcc aaccaaatat cttagttatt aatccaaact ttaaagtgga
     9601 attaatcgct actagctatt ttttttagcg gtgacgttat gaagcagtca agttgtttta
     9661 ctagcggaat agtgttttag cggtgatgta ttctttcctg aattttcatc tagcggtcca
     9721 aatgtgactt agcggtgacg attgaatttg atggttgaat attagcggac ctgtacttag
     9781 cggtgatgga aaacatcttg cttggtaact agcggaaaag tggtattaga aagtcgtagt
     9841 tgtggaacta aaacacaacc gaagtttgtc ttaaacttag tggtgatgac agtcgggcat
     9901 aagctgtctg aacggtttga gattcgcttc ctttgttatg caaagagaat gtcacaagtt
     9961 gataactgtg aaaacgtacc aatacgttct attggcgtta ggcggtaacg tgaaactcca
    10021 atatgagccc tgttctactt cagtagacag ggctttccta tttggcgctg attttcgttt
    10081 taagcaactt tttataaaaa agctacctga catcataaaa atctgtaatc aggctaaaaa
    10141 tcgcgaatac aagcgtctaa tcgggatata tagagcgagt gtctacgagc gaactatgaa
    10201 aagttgcgcc tagactccct gaaaaactgt tttttacagt tttagatcct tgaaaaactt
    10261 agatcacatg taaaattttg aatctaggtc gagcgaagcg agcaaaaaag caccatgagc
    10321 gaagcgaatt ccgagttgct tttgcttttt tttaaaagtc acggaaacat tgaccaaaaa
    10381 attgcccctc gaatcgagcg aaagcgagat tcaatagagt ttgagcgtag cgaaaaccaa
    10441 gggcaatttt tcatgattta agtttttaat tatttttaat gtttttaata ttcttttaac
    10501 ctttctctaa gccttataca tagggctttt ggaacgcaat atagcaacgt ttgctcggtg
    10561 atatagcaac gtttgctcgg tgatatagca acgtttgctc ggtgatatag caacgtttgc
    10621 tcggtattta acaacataaa atatccttgt tgcttctttt ttttaaaaaa atactattaa
    10681 tcaataagtt aaataaaaat aaataaaata attaacattt gcagtatgtt aaatctgcgg
    10741 tatatgacaa cattttctcg gttgtatata tttaataaat tttatattaa agtattgaaa
    10801 tttatcttta ttttttgatt tttaaaggtg agaaattttc atatcttttc taggatcgta
    10861 cattttacga tctaatattt gacaactata aaatataatg ttgttataac aacatatatt
    10921 ttatagttgc tattttttgt tcttatgaca gatctcgttg ttaaatctaa taaattaata
    10981 ctagcactac agtctttaac tttgtctgaa gctaggctaa ttcaattagc aattattgat
    11041 gcaagagaaa caggaaaagg acttactact gaacatccac ttgagttgaa tgctgcccga
    11101 tatgctaaag catttaatac atcgttagat gcagcatact taagtttatc tgaggcagaa
    11161 gaaactcttt ttaagcgtca atttactatt gagaatgatg atggttcatt aactaaaagt
    11221 cgatggatac aagatgcaaa ttacaaaaaa ggtgaagggc gtattttact taccttaact
    11281 cgtgtactca tagagcaagt gactaacatc gatggttttg aaacatattt caccagttat
    11341 tatttaaaac aaactgcaaa atttaagagt atttatgcag ttcgactata tgagttatta
    11401 aaacaatgga aaagtgttgg ttatacacca acttttaata tagataaatt cagggaacag
    11461 cttggtatcg gtattaatga atactctaga gttgaagctt ttaaaagacg tattttagat
    11521 gctagcattc aacaaattaa taaattttca gatatagagg tagagtatca acaacacaaa
    11581 aagggacgat caattgtagg tttttcattt acctttaaac ataaggaaac tcagaaaaac
    11641 agcaaaataa acaatgtaat aaatgagctt acagataagc aaattttatt attttctaat
    11701 aagttagctc ataacatacc tttctcatcc aaatacagta aacctgggga aagttatgag
    11761 gaatttgctg aaagactagc taacgaatta attgatccac aaaaagttaa attgtataag
    11821 ccttatttag aagaagttgg cttcaagtca taaaaatcct tcaatataaa tttgataaaa
    11881 aaaggcatgg ctattaagat tgtttgttaa gtaagttacg taagttacat aagaattatg
    11941 attattttag tcggtggcga aaaaggtggt tcaggtaaaa gctgtttggc acaaaaccta
    12001 gctgtttacc ttcaacaaaa aaaaagagac atattgcttc tagatgcaga tccacaaggt
    12061 acaactaccg attggattaa agaaagagat gaaaacccag atttaataaa cattccatca
    12121 gttcaagctt caggaaacat tcgccaggtc cttgacgatc tctctcgacg ttatcaagac
    12181 attgttattg atgctggtgg ccaagactca gaggctctcc gttcggcaat gacaattgcc
    12241 actcatatgc ttttaccatt ccgtcctaaa cgtagggatt taaaaacttt agtacacgta
    12301 gagcaactaa ttaagttagc aaaagctgtt aacccggaat tagtcactaa agcagttatt
    12361 actcaatgcc cgacacttcc tagtcaagct caacgtattt tagatgctaa agatgcttgt
    12421 gaatcttttg ggttggtagc tctcaatcaa ttcacgacaa atcgtaatgt atatgatgat
    12481 gcagatgaaa atggattatc ggtttttgaa gtggattctg atcctaaagc aaaagctgaa
    12541 attgagagaa tagttataga gtttttagga gaactatgat ggctctaaag ggtctcaaaa
    12601 aagctgatca agatactaaa cagcaagaca tagataactt cataagcggt gcatctaagc
    12661 gggttaaaac attagaaaaa tctcaacaaa aatatcttcg attgacattt tctttaacaa
    12721 aagatgtcga taaagaaatt gatgatcttc tcgttgattc taaagtagcc cgtgccaata
    12781 gatctattat tgttaaagct gcaattcatc atttacaaca gcttccaaag gatgagctaa
    12841 aaaatcttat cttaaaagaa ttaaattaga taaaagttat taaacttacg taagtttaat
    12901 aaattacgta agtttaataa cttaataaat ttttcttgta ttttgacatt tcctaccaca
    12961 cattatttaa aataatattt taattacaaa tacttacaac cacaacatgt gatttacctt
    13021 aaatattaat aaatctgtaa attaagcagt aaaaactcat atatacagaa aaaaataggc
    13081 aaatttccct attttaagta aaaaataggt cattggatca ataaatccat caaaaatatt
    13141 acttaactta cgtaagttaa gtaatatttc tgatggattt tgacatttct tcccacgtga
    13201 gattttaaaa taatatatta caaatactta aaataggctt tgttgcaaaa aagaaataag
    13261 caattgattt tattgacttt aattttatat aaaaaataaa tcaaaacctt taaaatcata
    13321 tagttatgaa atctttatgc aacaaatctg tacatagatc aaagctttgg cgttagggat
    13381 gttggctctg ctcatataag aacataatta aaagccatat tccaaactgc caagaaatca
    13441 aacaacaaga gtcaaaagta aattggaaaa cagtcgttga gtttcctgct tcccaatagt
    13501 ctaaatcaag gctgtagttt tttccgcccg attatcctag ttcggttttt tttatgccca
    13561 aaaattttaa ttcaaccctt gtacttatag gctatagcct aacggaacaa tttaagacct
    13621 aataaaatca gacttaaaac cgtatctata gacttaattt tacatttcag tacaatatgt
    13681 atctataact ccaatatcta acggtacaaa atgcctaatc aggttggcta catccgagtc
    13741 agttcatcgg aacaaaatac ggaacgtcaa ttggatggaa tccacctcga taagaccttc
    13801 atagagaagg caagtggggg tagccgtgaa cgtcctcaat tgctcgctat gctcgaatat
    13861 ctgcgtgatg gtgatacggt gcatgttcac agtattgacc gcctggcacg taatacgaat
    13921 gatctgaacg acattgtgaa cagcctgaat gatcgtggca taaccatcat ttttcataaa
    13981 gaaaatctga cctttagcca tgatgttgca cagtcggcaa taaataagct catgttccaa
    14041 atgcttgctg catttgcaga atttgaacgc tccatgatcc gtgaacggca aaaggaaggt
    14101 attgctaaag ccaaagcgaa aggcttatat aagggcagaa agcgtaaagt tgattatgca
    14161 gaaatacaaa aagccatgca ggaagatggc tcgactttta gaagtgtggc aaaacagttt
    14221 ggagtaggga ttgctacagt tcaaagggca gtagggcaaa agccttaatg atgaccagtc
    14281 atcaaggatc ggagaatatt ttatgcagct gtgagttccg taattttctt tttcaacact
    14341 gatacttgta cggctgaaga tcgaaagcta tttctgcatc tgcattgaag gctgttaata
    14401 ccccggcttt tggagccgtt tttttagccc ctatgattaa ccacgattgt ttaactaata
    14461 tgaacaaagt cgtgaaacat tggtcaaaga ataggcgtgg aacgtgaaaa atcataaaaa
    14521 aagcccacca gagggtatgt gggctaaaat catgtaacta cagcattgat ttctaaagat
    14581 aattataacg catttcgtat aaggtgtatt atgttaattt tagcttttct ctataattac
    14641 tatcttaagg ccc
//
