LOCUS       NZ_CP055283             9444 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter sp. NEB 394 plasmid pBspH1, complete sequence.
ACCESSION   NZ_CP055283
VERSION     NZ_CP055283.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15195663
            Assembly: GCF_013374795.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. NEB 394
  ORGANISM  Acinetobacter sp. NEB 394
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Morgan,R.D.
  TITLE     Acinetobacter species H NEB394 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Morgan,R.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2020) Genome Biology, New England Biolabs, Inc.,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013374795.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 04:29:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,807
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,431
            CDSs (with protein)               :: 3,431
            Genes (RNA)                       :: 113
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 84 of 263
            Pseudo Genes (incomplete)         :: 172 of 263
            Pseudo Genes (internal stop)      :: 55 of 263
            Pseudo Genes (multiple problems)  :: 43 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP055283.1.
            Bacteria and source DNA available from Richard Morgan, New England
            Biolabs, Ipswich MA 01938.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Acinetobacter sp. NEB 394"
                     /mol_type="genomic DNA"
                     /strain="NEB 394"
                     /isolation_source="soil"
                     /db_xref="taxon:2743575"
                     /plasmid="pBspH1"
                     /geo_loc_name="USA:Massachusetts"
                     /collection_date="1986"
                     /collected_by="Daniel Hall"
                     /note="This strain was originally classified as a Bacillus
                     species, and is the source of the commercial restriction
                     endonuclease BspHI; however from the sequence data it is
                     clearly an Acinetobacter, not a Bacillus so it was
                     submitted as Acinetobacter species H (NEB394), formerly
                     known as Bacillus species H (NEB394)."
     gene            1219..1434
                     /locus_tag="HUK62_RS17150"
                     /old_locus_tag="HUK62_17150"
     CDS             1219..1434
                     /locus_tag="HUK62_RS17150"
                     /old_locus_tag="HUK62_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_176538536.1"
                     /translation="MDNYFLESLGSKIKILRKSRGVSQEDLAKQSEITRTYLTQIENGK
                     VNPSIGKIKKISENLNISLCEMFKDI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1456..2601
                     /locus_tag="HUK62_RS17155"
                     /old_locus_tag="HUK62_17155"
     CDS             1456..2601
                     /locus_tag="HUK62_RS17155"
                     /old_locus_tag="HUK62_17155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538537.1"
                     /translation="MSKLSDVFKYISFYRSAGHQIGRKVGDMLEVLTYGALHYDQNLKK
                     RLHIEPNLYGFSDAGHKVEFLITKDVNENLLKGGSVTNLENYIGFIECKKVGVEQTVST
                     SFKNKFKDYENKQTKKYDLKLDSIFNIGFSSHGMNRHKLSVSFANCDNNLFINVKNEIN
                     NEIIFNEQVKDHYRLIVAQCSDNSIDIIGNSRSLREFNLPLNNCRILEISNFNLQENRI
                     SLVLNNCLAGPQTPEKAKQASFVALDVRKKRFGSFDKVDDPSFKSILVLTEFAHWERKS
                     RNMISACIDINLVVPDSILIEAFEVFNQYFERNGATVSNLYDLITKDNFEKNKEIQDLI
                     MSILTEYDGKIFQQLKSDGTHIEELVSLNYLNNSLSIISER"
                     /name="hypothetical protein"
     gene            complement(2585..3820)
                     /locus_tag="HUK62_RS17160"
                     /old_locus_tag="HUK62_17160"
     CDS             complement(2585..3820)
                     /locus_tag="HUK62_RS17160"
                     /old_locus_tag="HUK62_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_176538538.1"
                     /translation="MFNLSSENLTPIYQKFPPLIEEINGEEIPTFQWDIPYTIKDISYL
                     THSHYRYYGKFPSVLAGKIIDLFPPKDKNSYILDNFCGSGTTLVEAKLRGINSVGLDIN
                     WISALASNVKTKHININKIRNLHDSILFLYKQYVSKPFDSNLDVLTSFEEKWFVPENVK
                     DLKILQNILLNLEASDELDFIVVAFLGIIRRVSKAYDGEVRPHINKEKKVREVISAFSK
                     KINDMISDHIKYMDITNSNTFATCYVGNNLDLPDTIKEKNIYLVISHPPYLNSFNYSPI
                     FSLELYWGKVFEKNYSPLVSLYKEEMKAHPASESITEQYFNHLRDCYTQTFNIQKNGDY
                     LAVVIGDCTRKGELIPVLKNTQKIIEEIGYKTIMLNYRTTHYGLGKYAYKHRADYHDDI
                     NGKKDGILIFQK"
                     /name="DNA methyltransferase"
     gene            complement(4141..4692)
                     /locus_tag="HUK62_RS17165"
                     /old_locus_tag="HUK62_17165"
     CDS             complement(4141..4692)
                     /locus_tag="HUK62_RS17165"
                     /old_locus_tag="HUK62_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_176538539.1"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKGSDGLIDFTEALRV
                     FGEPQKKETSVNQSQSINSQNLTEVDLLKRQVDILEKQLNQAVLRENQSLERESFYQEQ
                     IEAMQRLLEAPKPPKVEDPTPAAAPQETVELAKEEAPVEAEIQTQPKDDGLTTLVEKEN
                     KRIPEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            6521..6838
                     /locus_tag="HUK62_RS17170"
                     /old_locus_tag="HUK62_17170"
     CDS             6521..6838
                     /locus_tag="HUK62_RS17170"
                     /old_locus_tag="HUK62_17170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538540.1"
                     /translation="MVAQVPTTIRTKALVALFTQLERIEKRLTRNTIKDDIALTPFSPL
                     RLRYRAIWHGSTLHAEVDRIRSIHTGRQPACQKSNELEVSQTPKTFLQRRKKQRELVAP
                     C"
                     /name="hypothetical protein"
     gene            7173..8471
                     /locus_tag="HUK62_RS17175"
                     /old_locus_tag="HUK62_17175"
     CDS             7173..8471
                     /locus_tag="HUK62_RS17175"
                     /old_locus_tag="HUK62_17175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176538541.1"
                     /translation="MKVIFYTTSNKFFMSAILNCVSKGKATFYRKIFIDIDQAEKGIAS
                     LIKRYNVGLGSATNNKNFACNGHLVIQYNQDLRQKRQVIICILITLPQEIRDTSLLYQK
                     LVADLQVEPAPTPRKRKNLAIAYQKDFIKKDFKERDGSENREEFHSLEHEKQRIYIYNV
                     DSVVKLELRKSDYPKAKVRQMQKYADEQALKQGKPPRKISGSRWTWHLTPVYMDLIFSV
                     AKVEIDKKILIKKDLIKKTVTQSKANMPTKDVIKRQARLEPSEYREAISNVAFMVDTIP
                     MFGGTLADVGQVVQKLEKQFNRGQAHKAKGAWATQKKVHLPITEAFDIQRLKKEASYST
                     RIDWKYHSFSSLYEAVETFADHEDMDEKYKQKREEDVLKFYEKGQARIARRTKQQNNDR
                     RIKLSRERVQKLIQKRFSKWKDKVRGIHKDQKQ"
                     /name="hypothetical protein"
     gene            complement(8533..9429)
                     /locus_tag="HUK62_RS17180"
                     /old_locus_tag="HUK62_17180"
     CDS             complement(8533..9429)
                     /locus_tag="HUK62_RS17180"
                     /old_locus_tag="HUK62_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729701.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_176538542.1"
                     /translation="MQNNIQECFKSMSVNEKRLLILSSPLARTQDATEKDAIIITAEDF
                     SKECGITQHSAYAQLEKASKSLIKRYFSYHNEKNKKVLSNWVIDCTYESGGIAIRFPEV
                     VLFMLKEFDKVNPYTKYKKDIILRLKRDYSFDLYHLAKKFQAMGRFEISLEDFRNELGV
                     PDSYNDLSNLKKRVIQPSLDEITEQTDINVTYENIKSGRAVVGFRFTVKEKSKLKAIEG
                     GDQNTVHMFCKMTDSQINTYSSILSNVHSISDLAGNKDYQAFAVWISNILRDPKSVREN
                     TAKRIFKALRTETDFKG"
                     /name="replication initiation protein"
ORIGIN
        1 agggtaagtt gttattttca taaagctaaa aaccaaaaaa gtacaataga tataatgtac
       61 ttaataatag caatataaga tcaaaaaatc tataaaagta cacaatctac aataaacttt
      121 ttatgggttt tcgctcaaat tttgagtccc ctatttgtac ttttttttaa taaaatgcaa
      181 tccagtatcc aatctacaat gtgcttggtt ttgggtttag atccaaattc gagtcttttt
      241 tgtgcttttt tagcaaaacg caacaaagaa tctagtttgg aatgtacttt gtattgggtt
      301 ttcgcacaaa atatacccat attatttaat ttactcattt aaagtacgat gtatttattg
      361 tacttttcag tccctcaaaa gtacttagtt agtccctcaa aagtactttt taaaacgcta
      421 aaagtccctc aaaagtactt agttagtccc tcaaaagtac ttttttaaag tctgaaagca
      481 ttgatatata tggcttacat ggggggctaa aagtctttta aaagcttatt aaaagaataa
      541 aagaaaacag gggagtgaaa actgccctat gacactcgct aaagctcgtg gcattttgac
      601 ctcgctaaag ctcggtttaa ggggcagttt tgggttacga tcttgagagg attctaaaaa
      661 agtaaaagca actacggctt cgcttcgctc agaagctttt tttactcgct tcgctcgatc
      721 tagaagcaaa tataagcaat tgatttactg cttttttgaa gcaaatgttt taaaaacggt
      781 ttttcagagg gtctaagcgc aaattttggt gtggtcgctc gtagacactc gctcggaaag
      841 tgcagtcaga tgcaaaaaaa gagagatagg gatttagaga cccataaacc atatattgtg
      901 attaaaagta gtcgtattct gctctatttt gcgctgtatg gcatttttta tttactttct
      961 atacccaact agcacaattg atttaaagtg gcttaaaacg caaatggagg gattttatca
     1021 agggtggagt tttcggataa tttttcaatg tggtttcaat acaatttaaa taagcaaaaa
     1081 aacagcaatt cattgaaatt cacgctaagt atagaaattg cgtataagag attttatgtt
     1141 aaatttttag ttgatttatt aggggatata gattttttca cgtgtatcat gttctgagat
     1201 atgtaatagg gcgatatagt ggataattat tttttggaaa gtcttggtag taaaattaaa
     1261 attttacgta agtctagagg ggtttctcaa gaagatttag caaagcaatc tgaaataaca
     1321 agaacctatt tgactcaaat tgaaaatggt aaggttaacc catcaattgg aaaaataaag
     1381 aaaatttctg aaaatttaaa cataagcttg tgtgaaatgt ttaaggacat ataatccttt
     1441 attttaggaa caattgtgtc gaaattatcc gatgttttta aatatatatc tttctataga
     1501 agtgctggtc atcaaatagg gcgaaaggtt ggggatatgt tagaagtgct aacatatgga
     1561 gctttacatt acgatcaaaa tctgaaaaaa agattacata ttgaacctaa cttatacggc
     1621 ttttctgatg cagggcataa agttgagttt ttaattacaa aagatgtaaa tgagaattta
     1681 cttaagggag ggagtgttac taatctagaa aattatatag gttttattga atgtaaaaag
     1741 gtaggagtcg agcaaacagt ctcaacttca tttaaaaata aatttaaaga ttatgaaaat
     1801 aaacagacta agaaatatga tttaaaatta gatagtattt ttaatatcgg tttttcaagt
     1861 catggtatga atagacacaa gttatctgta tcttttgcaa attgtgataa taatttattc
     1921 attaatgtaa aaaatgaaat caataatgaa atcattttta atgaacaagt caaagatcat
     1981 tatagactta tagttgcaca atgtagtgat aatagtatag atataatagg aaatagtcga
     2041 agtttaagag aattcaattt accattaaat aactgtcgta tattagaaat atctaatttt
     2101 aatttacagg agaatagaat atctttagtt cttaataatt gtttagctgg tccgcaaaca
     2161 ccagaaaaag caaaacaggc ttcatttgtt gctttagatg ttcgtaagaa gagatttgga
     2221 tcatttgata aggttgatga tccaagcttt aaaagtattt tagtgttaac tgaatttgca
     2281 cactgggaaa gaaaaagcag aaatatgatt agtgcttgta ttgatatcaa tcttgtagta
     2341 ccagatagta tattaatcga ggctttcgaa gtatttaatc aatattttga aagaaatggc
     2401 gcaacggtat caaatttata tgatctaata acaaaagata actttgaaaa gaataaagag
     2461 atacaagatc ttattatgag tatattaaca gaatatgatg gtaaaatttt ccaacaactt
     2521 aagtcagatg gtactcatat tgaagaactt gtatctctaa attacttaaa taatagttta
     2581 tctattattt ctgaaagata agaattccat cttttttacc gttaatatca tcatggtagt
     2641 ctgcacgatg tttgtaagca tatttaccta aaccataatg tgtggttcga taatttaaca
     2701 taatagtttt atatccaatt tcttctataa ttttttgggt attttttaat acggggatga
     2761 gttcaccttt acgtgtacaa tccccaataa ctacagcaag atagtcgcca tttttttgaa
     2821 tattaaaggt ttgtgtataa caatctctta gatgattgaa atattgttca gtaatagatt
     2881 cactcgcagg gtgagctttc atttcttctt tatatagaga aacaagggga gagtaatttt
     2941 tttcgaaaac tttaccccaa tataattcta agctaaagat aggagaataa ttgaagctgt
     3001 ttagatatgg cgggtgacta attactaaat atatattttt ttctttaata gtatcaggta
     3061 aatctaagtt attaccaaca tagcatgtag caaaggtatt agagttagtg atgtccatat
     3121 atttaatatg atcactaatc atgtcattaa tttttttaga aaaagcagag atcacctccc
     3181 taactttttt ctctttatta atgtgaggtc ttacttcacc atcataggct tttgatactc
     3241 ggcgtataat gcctaaaaat gcaactacaa taaaatctag ctcatcagat gcttctaaat
     3301 ttaaaagaat attttgtaaa atttttaagt ctttgacatt ttctgggaca aaccattttt
     3361 cctcaaaact ggttaataca tctaaattac tatcaaatgg tttcgacaca tattgtttat
     3421 atagaaaaag aatgctgtca tgtaagtttc taattttatt tatatttata tgtttggttt
     3481 tgacatttga tgcaagagca gaaatccaat tgatgtctaa accaactgaa ttaattcctc
     3541 taagctttgc ttcgacaagg gttgttccag agccacagaa attatctaga atataactgt
     3601 ttttatcttt tggtggaaac aagtcaataa tttttccagc tagaactgat ggaaatttac
     3661 catagtatct atagtggcta tgtgttagat atgagatatc tttaatcgta taaggtatat
     3721 cccactgaaa agttggaatt tcttcgccat ttatctcttc tattagaggc ggaaacttct
     3781 gataaattgg ggtaagattt tcactagata aattaaacat agtagctctt tgtgttaaat
     3841 atatttaaca ttatatcatg atttagtctg atagtaagta tattagtcag gcaattaaaa
     3901 aaattattct taaaactgat tatcacaact tttattctac tcattattta agtttctcta
     3961 aacttaacat aatacagctt atgcgaaatg caatttttta tttgttataa ttcataatct
     4021 tatgtcgatg tgaaaagccc aactcagctg atttgggctt ttttgttgat tcttaatgtt
     4081 ccacgcctat tatttaagca atgtttcacg actttgttca tattagttaa acaatcgtgg
     4141 ttaatcatag ggtctaaaaa aacggctcca aaaacccctt ttctcaggct ctggaatccg
     4201 cttattttct ttttcaacca aagtagtcaa tccgtcatct ttaggctgag tttggatctc
     4261 ggcttctact ggagcttctt ctttggctaa ttcaaccgtt tcttgaggtg cagcagcagg
     4321 tgttggatct tctacttttg gcggttttgg ggcttccagt agacgttgca tagcttcgat
     4381 ttgttcctga tagaacgatt cacgttctag cgattgattt tctctcagca ctgcctgatt
     4441 taactgtttt tccagtatgt caacttgacg ttttagtaag tcaacttctg tcaagttttg
     4501 actgttaatt gattgacttt gattgacact agtctctttt ttttgtggtt ccccaaaaac
     4561 tcttaaggct tctgtaaagt caattaaacc atcagatcct ttagataaat ttcctttgtt
     4621 tatatgtgca tatatggcct gtcttgaata tccgtaaagc ttggccaact ctgaaactga
     4681 cagttttttc attatgtaaa ccagttttca acctgtgtta atacttgact gttaacttaa
     4741 caatttcaat taacaatgtc atttaaatct tacttttctg atctgagtaa aagaattaac
     4801 tccttaacac tttaaatcac cctcatttgc tctgtattgg atttttatag acattccccc
     4861 tttataggat ccataggttt taaaaagtag cttaaaacgc aaatggtgga gttttatcag
     4921 ggggtagggt tttcgaataa ttttttcaat gtggtttcaa tacgattcaa ataagcaaaa
     4981 aaaaccgcaa ttcattgctg aattgcggtt ccgaaaaatt tcttctatcc aaacctatgt
     5041 gtgtttttaa agtctggtag gttttttctt gctaacaggt gccatattcg gatctgccac
     5101 acaattcaca gtcccaacat ttttttatct gccaacgtct tggatcagac ctgccctgtt
     5161 gccagtagct acccgaagaa cgctatatca tttttgagag gaagtgaata tctgtgagtg
     5221 aattattttt tatctcatgg aaatagaaaa gaccgtacta caaaggtgca gtacggtctt
     5281 taaaaaatga taaaacaata gatctctcta tgtgtttaga attaatcaat tcacttgaga
     5341 aagtgagtgg attaatttga tgaaatgtta atttaatcaa gattttaatc ttggatgtat
     5401 tgattagagg gatggataga aagttggtga ctaatcaaca aaaaatcgtt ccataattaa
     5461 tactgttaaa ttttacagtt cgttggatag tcctcgtaaa gctgaggatt gctacacaga
     5521 atatgtgtgg atttcactct gccatctctg acagttcatc tatttaacga aaaggtaaag
     5581 ccgatgaata actctctcat gcttacagga ctgagatcct gatgattttg ttacctaaaa
     5641 tcataaaggt ctgaacgaga aagcgtcaga ctttgcttgt aagccaagcg actacgtttg
     5701 cttttgttgc aagtaccaaa gaaataccat ttcatactcg attaaagatc taccaaatct
     5761 taagttgagc atctcaaaac cacctatttt gaaattggtt acttgcagtc cttcttttgc
     5821 tttttcacta cacacacgct cgagttttcg tcctactgac ttgtacgatt tttgtctatc
     5881 ttgttgtaaa actactaaaa ccaagatagt taacgcattc gccaataata gtgggtttcg
     5941 cccccgccta ctctcccgat ctcccgatgg ataaatgatc ctttgcttgc tttgcgtcaa
     6001 atgaagacat tcgcaagaat taaaatcaaa actgtttttc ttagcttttg actaaaaaaa
     6061 tcaactgtag tggctattat gaaaaatcat aactacagtc acagctccat cttccacact
     6121 tacaccaaaa tctactatgt acccttcgga tagcttatag cccttaattc cactccccga
     6181 tcaatcaatt gatcttcgat ttcccctgtc gtcgttccgt aaacaggctt ggcaagaatt
     6241 aaaaacaaag ttatatccca ttgataccca ctcgcacccc tacactacaa acaacgtagt
     6301 gcttaggttc tgctagagtt gagctattcg tgctgattgt gtctttccac acaaccaaaa
     6361 agtttcgacc taatgatgat catctacttt ccacagatcc ccgagcttct gctctttcac
     6421 tgtgacactc cctctcgtgg cattgcatct gcggttattt tccctacaga aacacggctg
     6481 aatcgacaaa aaaatcgtct acagcttcga ttatggtttc atggtcgccc aagtaccaac
     6541 caccattcgc acaaaggcat tggtggctct gttcacacaa ttggaacgga tagaaaaaag
     6601 gctgacacgc aacaccatca aagatgatat tgcgctgaca ccttttagcc cccttcgttt
     6661 gcgataccga gccatttggc atggcagtac gcttcacgct gaggtagatc gtattcgttc
     6721 gatacataca ggtcgccaac ctgcatgcca aaaaagcaat gaattagaag ttagtcagac
     6781 tcccaaaaca tttttacaaa gaagaaaaaa gcaaagggag ttggtagctc cctgttgaac
     6841 aaagagctag acctaacttc taaaaaagaa gaaccaattt ttaaagaata cggtttaaca
     6901 agccgaattt atcaccctat tcagggcggg gcaatcagag tttcatcaaa ttacaggaac
     6961 gacttgatta caccctgatt gccccgccac gaagcttcgc ttcgtaagaa aagccagctt
     7021 cgctgacttt tcttttcaaa atccttgctt tgcaaggatt tgtttttaaa tgtaaattta
     7081 ttaaaaaaag taatacctga aattttttaa attattacat ttccactatt acaccttttc
     7141 aaaattttat tgcttggtga gcaaatgggg aaatgaaagt gattttttac accacttcaa
     7201 acaaattttt tatgtcggca attttgaatt gtgttagcaa aggaaaagcg actttctatc
     7261 gaaaaatatt tatcgatatt gaccaagctg agaaaggaat tgcgagttta attaagcggt
     7321 acaatgttgg tttaggttct gcaacaaaca ataaaaactt tgcctgtaat ggacaccttg
     7381 ttattcagta caaccaagat ttaaggcaaa aaagacaggt catcatttgt attttgatta
     7441 ctctacctca agaaatccga gatacttctc tgctgtatca aaagctagtt gcagacttac
     7501 aagtcgagcc tgcgccaact ccacgaaaaa ggaaaaacct tgcgattgct tatcagaaag
     7561 actttataaa aaaagacttc aaggaaaggg atggatcaga aaatcgtgaa gaatttcata
     7621 gcttagagca tgagaaacag aggatctaca tctacaatgt tgattcagtc gtaaagctag
     7681 aattgagaaa gtcagactat cctaaagcca aagtaagaca gatgcagaag tatgccgatg
     7741 aacaggcttt aaaacagggt aaaccaccta gaaaaatcag tggatctcga tggacttggc
     7801 atctcacgcc tgtttatatg gatctgatat tttctgtagc caaagttgag attgataaaa
     7861 agattttgat taaaaaggat ttaatcaaaa agacggttac tcaatcaaaa gctaatatgc
     7921 cgactaagga tgtgataaag cgtcaagcaa ggttagagcc ttctgagtac cgtgaagcga
     7981 tttctaatgt cgctttcatg gttgacacca ttcctatgtt tggcggtact ttggctgatg
     8041 tggggcaagt ggtacagaaa cttgaaaagc agttcaatcg agggcaagca cataaagcta
     8101 aaggtgcttg ggctactcag aaaaaagttc atttgccgat cacagaagct tttgatattc
     8161 aaaggctaaa aaaagaagca tcatattcga cccgtatcga ttggaagtat cattcctttt
     8221 cttctttgta tgaagctgtt gaaacctttg cagatcatga agatatggac gaaaagtata
     8281 agcaaaaaag agaagaagac gttttaaaat tctatgaaaa ggggcaagca aggattgcaa
     8341 gaagaaccaa acagcagaac aatgaccgca ggattaaact cagtagagaa cgggtacaga
     8401 aattgattca aaagcggttt tcaaaatgga aagataaagt ccgtgggata cataaggatc
     8461 aaaagcagta agtggatctg gccaaccaca ccaggatttt ttcaaaatct tggtgtggtt
     8521 ggcaggtcaa acttagccct taaagtctgt ttcagttcgc aaagccttga aaatgcgctt
     8581 tgctgtattt tcacgaaccg attttggatc tcgtaaaata ttcgaaatcc ataccgcaaa
     8641 tgcttgatag tccttgttgc ctgcaaggtc actgatgctg tggacatttg aaagaatgga
     8701 gctgtaagtg ttgatctggc tgtcagtcat cttgcagaac atatgaacgg tattttgatc
     8761 tccaccctca atcgctttca gcttcgactt ctccttgact gtaaaacgga agccaacgac
     8821 agcacgcccc gattttatgt tctcgtaggt tacgttaata tctgtctgtt ctgtaatttc
     8881 atctaatgat ggttgaatta cacgtttttt taagttgctt aggtcattgt atgaatcggg
     8941 cacgcctagc tcatttctaa aatcttctag ggatatttca aatctcccca ttgcctgaaa
     9001 ttttttagct agatgatata agtcgaaaga gtagtccctt ttcaatctca gtatgatgtc
     9061 ttttttgtac ttggtatagg gatttacctt gtcgaactct ttgagcataa ataagacaac
     9121 ttcgggaaag cgaatagcaa tccctcctga ttcgtaagtg cagtcaatta cccaattaga
     9181 taagaccttt ttgttttttt cattatgata agaaaaatat cttttaatca atgacttact
     9241 tgctttttct agctgtgcat atgctgagtg ctgtgtgatc ccacactctt tcgaaaaatc
     9301 ttctgccgtg atgataatag cgtctttttc cgtagcatct tgtgtacgtg caagagggct
     9361 agaaagaatc aaaaggcgtt tttcatttac actcatgctt ttaaaacact cttgaatgtt
     9421 attttgcatt acgacccatg atgg
//
